BLASTX nr result
ID: Coptis25_contig00010289
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Coptis25_contig00010289 (2526 letters) Database: ./nr 23,641,837 sequences; 8,123,359,852 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CBI22707.3| unnamed protein product [Vitis vinifera] 623 e-176 ref|XP_002271744.1| PREDICTED: uncharacterized protein LOC100264... 623 e-176 ref|XP_003533181.1| PREDICTED: uncharacterized protein LOC100780... 615 e-173 ref|XP_002318812.1| predicted protein [Populus trichocarpa] gi|2... 607 e-171 ref|XP_003549040.1| PREDICTED: uncharacterized protein LOC100810... 606 e-170 >emb|CBI22707.3| unnamed protein product [Vitis vinifera] Length = 1040 Score = 623 bits (1607), Expect = e-176 Identities = 339/556 (60%), Positives = 407/556 (73%), Gaps = 1/556 (0%) Frame = -2 Query: 1667 LSKMVQVPKQVEGNYLNENGSTETNPQLIFSDSYTNEPYMNTELGGSNPYFDSLVPENSI 1488 L K + V+ + LN +GST + L + Y + + ++ + + +S + ENS Sbjct: 435 LEKDIPNKSYVKLHDLNASGSTSSTSALPYPFDYDQDVNLQNKIQRNRSFLESPIAENSF 494 Query: 1487 TSSGIPAPSLVSAALQXXXXXXXXXXXXXXXXXXXXXXXXXXXVIEADAHPGDLCTRREY 1308 +S+GIPAPS VS +L+ VIE D P DLCTRRE+ Sbjct: 495 SSAGIPAPSAVSESLKVLPGQVVVPAVVDQVQGQALAALQVLKVIEPDVQPSDLCTRREF 554 Query: 1307 ARWLVXXXXXXXXXXXSKVYPAMYIENVTELAFDDITPEDPDFAFIQGLAEAGLISSKLS 1128 ARWLV SKVYPAMYI N+TELAFDDITPEDPDF+ IQGLAEAGLISSKLS Sbjct: 555 ARWLVSASSVLSRNTVSKVYPAMYIGNITELAFDDITPEDPDFSSIQGLAEAGLISSKLS 614 Query: 1127 RTDMLGSSDEE-QDPSLFFPESPLSRQDLVSWKMALDKRWLPDVDKKIVYQSCGFIDIDK 951 R D+L SDEE Q P F P+SPLSRQDLVSWKMAL+KR LP+ DKK++YQ GFIDID Sbjct: 615 RRDLLSFSDEEDQSPFYFSPDSPLSRQDLVSWKMALEKRQLPETDKKVLYQVSGFIDIDS 674 Query: 950 INPDAWPALVADQSAGEEGIMALAFGYTRLFQPYKPVTKGQAAIALATGEAADIVSEELA 771 INPDAWPALVAD SAGE+GI+ALAFGYTRLFQP KPVTK QAAIALATGE++DIVSEELA Sbjct: 675 INPDAWPALVADASAGEQGIIALAFGYTRLFQPNKPVTKAQAAIALATGESSDIVSEELA 734 Query: 770 RIEAESMAETAVAAHSALVAKVEKDINASFEKELIMEREKIDAVEKMAQXXXXXXXXXXX 591 RIEAE+MAE AVA HSALV +VEK++NASFEKEL +ER+KIDA+EK+A+ Sbjct: 735 RIEAEAMAEKAVAEHSALVDQVEKELNASFEKELSLERKKIDAMEKLAEEARQELEKLRA 794 Query: 590 XXXXETNSLMRGQVAVESEMQVLSSLRHEVEEQLQSLMSDKMEVSFERERINKLCKEAES 411 + SL++ + A+ESEM+VLS LR EVEEQLQS MS+K+E+S+E+ERI+KL KEAES Sbjct: 795 ERDEDNISLIKERAAIESEMEVLSRLRSEVEEQLQSFMSNKVEISYEKERISKLRKEAES 854 Query: 410 ENQAITRLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARDRWEKHGIKVIVDDDL 231 ENQ I RLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARDRWEKHGIKV+VD++L Sbjct: 855 ENQEIARLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARDRWEKHGIKVVVDNEL 914 Query: 230 REDANAGVTWLTAGTHSTVDATVSRAESLVLKLKTLAGEIRGKSRFVIETIIFKITSLIS 51 RE+A+A VTWL +VD TVSRAE+LV KL + ++RGKS+ VI+ I+ KI LIS Sbjct: 915 REEASAEVTWLDTAKQFSVDGTVSRAENLVDKLNAMGSDLRGKSKDVIDNIVQKIIHLIS 974 Query: 50 SLKEWAANSARRAGEL 3 L+E A+ + EL Sbjct: 975 ILRELASKVGTQVREL 990 >ref|XP_002271744.1| PREDICTED: uncharacterized protein LOC100264485 [Vitis vinifera] Length = 985 Score = 623 bits (1607), Expect = e-176 Identities = 339/556 (60%), Positives = 407/556 (73%), Gaps = 1/556 (0%) Frame = -2 Query: 1667 LSKMVQVPKQVEGNYLNENGSTETNPQLIFSDSYTNEPYMNTELGGSNPYFDSLVPENSI 1488 L K + V+ + LN +GST + L + Y + + ++ + + +S + ENS Sbjct: 380 LEKDIPNKSYVKLHDLNASGSTSSTSALPYPFDYDQDVNLQNKIQRNRSFLESPIAENSF 439 Query: 1487 TSSGIPAPSLVSAALQXXXXXXXXXXXXXXXXXXXXXXXXXXXVIEADAHPGDLCTRREY 1308 +S+GIPAPS VS +L+ VIE D P DLCTRRE+ Sbjct: 440 SSAGIPAPSAVSESLKVLPGQVVVPAVVDQVQGQALAALQVLKVIEPDVQPSDLCTRREF 499 Query: 1307 ARWLVXXXXXXXXXXXSKVYPAMYIENVTELAFDDITPEDPDFAFIQGLAEAGLISSKLS 1128 ARWLV SKVYPAMYI N+TELAFDDITPEDPDF+ IQGLAEAGLISSKLS Sbjct: 500 ARWLVSASSVLSRNTVSKVYPAMYIGNITELAFDDITPEDPDFSSIQGLAEAGLISSKLS 559 Query: 1127 RTDMLGSSDEE-QDPSLFFPESPLSRQDLVSWKMALDKRWLPDVDKKIVYQSCGFIDIDK 951 R D+L SDEE Q P F P+SPLSRQDLVSWKMAL+KR LP+ DKK++YQ GFIDID Sbjct: 560 RRDLLSFSDEEDQSPFYFSPDSPLSRQDLVSWKMALEKRQLPETDKKVLYQVSGFIDIDS 619 Query: 950 INPDAWPALVADQSAGEEGIMALAFGYTRLFQPYKPVTKGQAAIALATGEAADIVSEELA 771 INPDAWPALVAD SAGE+GI+ALAFGYTRLFQP KPVTK QAAIALATGE++DIVSEELA Sbjct: 620 INPDAWPALVADASAGEQGIIALAFGYTRLFQPNKPVTKAQAAIALATGESSDIVSEELA 679 Query: 770 RIEAESMAETAVAAHSALVAKVEKDINASFEKELIMEREKIDAVEKMAQXXXXXXXXXXX 591 RIEAE+MAE AVA HSALV +VEK++NASFEKEL +ER+KIDA+EK+A+ Sbjct: 680 RIEAEAMAEKAVAEHSALVDQVEKELNASFEKELSLERKKIDAMEKLAEEARQELEKLRA 739 Query: 590 XXXXETNSLMRGQVAVESEMQVLSSLRHEVEEQLQSLMSDKMEVSFERERINKLCKEAES 411 + SL++ + A+ESEM+VLS LR EVEEQLQS MS+K+E+S+E+ERI+KL KEAES Sbjct: 740 ERDEDNISLIKERAAIESEMEVLSRLRSEVEEQLQSFMSNKVEISYEKERISKLRKEAES 799 Query: 410 ENQAITRLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARDRWEKHGIKVIVDDDL 231 ENQ I RLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARDRWEKHGIKV+VD++L Sbjct: 800 ENQEIARLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARDRWEKHGIKVVVDNEL 859 Query: 230 REDANAGVTWLTAGTHSTVDATVSRAESLVLKLKTLAGEIRGKSRFVIETIIFKITSLIS 51 RE+A+A VTWL +VD TVSRAE+LV KL + ++RGKS+ VI+ I+ KI LIS Sbjct: 860 REEASAEVTWLDTAKQFSVDGTVSRAENLVDKLNAMGSDLRGKSKDVIDNIVQKIIHLIS 919 Query: 50 SLKEWAANSARRAGEL 3 L+E A+ + EL Sbjct: 920 ILRELASKVGTQVREL 935 >ref|XP_003533181.1| PREDICTED: uncharacterized protein LOC100780360 [Glycine max] Length = 975 Score = 615 bits (1586), Expect = e-173 Identities = 369/756 (48%), Positives = 477/756 (63%), Gaps = 22/756 (2%) Frame = -2 Query: 2207 VDDINAGTSITQNGESASSHTDD-TNNTSNQEDKQIRXXXXXXXXXXXXXXXXXXXXXXD 2031 VDD + G+ + + ++ S DD T + S QED Q Sbjct: 182 VDDSDIGSQLIYDSKNPSDGVDDATKHISVQEDLQDELAFGNKLVFASESPVPLESENTI 241 Query: 2030 LVSGHANAFSLKD----PEVDPADAVPGLITEPKDNLVPNNLDYPPVSDDASA-YVSPDF 1866 NA+ +D P VD A++ L K+NL + P DDA +++ + Sbjct: 242 ---DSFNAYGFRDFDSNPNVDTAESTANL----KENLFNVDPGDAPNYDDAKPLHLNTEQ 294 Query: 1865 QNEISMSSGTEIFDISTDLSSGSGGLIPNESDDSVKSDLVAMPSWPVDSEEVVETET--- 1695 +EI+ SSG+ F S SS ++++ + S LV S + S+ E Sbjct: 295 HDEITSSSGSVSFGFSETYSSSG-----SDNETGIVSVLVNPESNNMISDPKFFNEAGQE 349 Query: 1694 ---ALSPQEDHNLSKMVQVP----------KQVEGNYLNENGSTETNPQLIFSDSYTNEP 1554 + S E+ +L+K+ QV + V GN L E S ++ + + TN+ Sbjct: 350 NILSASKNENLDLNKIPQVSAEGNEPSFEERSVPGNDLFEESSISSSVNTLVDEQVTNDN 409 Query: 1553 YMNTELGGSNPYFDSLVPENSITSSGIPAPSLVSAALQXXXXXXXXXXXXXXXXXXXXXX 1374 Y E+ +P S + GIPAPS+VSA++Q Sbjct: 410 YEVDEVKSKSPNSGSF-----FSVPGIPAPSVVSASVQVLPGKVLVPAAVDQVQGQALAA 464 Query: 1373 XXXXXVIEADAHPGDLCTRREYARWLVXXXXXXXXXXXSKVYPAMYIENVTELAFDDITP 1194 VIE D P DLCTRREYARWLV SKVYPAMYI+NVTELAFDD+ P Sbjct: 465 LQVLKVIEPDVQPSDLCTRREYARWLVSASSALSRSTVSKVYPAMYIDNVTELAFDDVIP 524 Query: 1193 EDPDFAFIQGLAEAGLISSKLSRTDMLGSSDEEQDPSLFFPESPLSRQDLVSWKMALDKR 1014 EDPDF+ IQGLAEAGLI S+LSR D+ S++E+ P F PESPLSRQDLVSWKMAL+KR Sbjct: 525 EDPDFSSIQGLAEAGLIESRLSRRDIQLSAEEDDSPFYFSPESPLSRQDLVSWKMALEKR 584 Query: 1013 WLPDVDKKIVYQSCGFIDIDKINPDAWPALVADQSAGEEGIMALAFGYTRLFQPYKPVTK 834 LP+ ++K++YQ GFID DKI+P+A PALVAD S+GE+GI+ALAFGYTRLFQP KPVTK Sbjct: 585 QLPEANRKVLYQVSGFIDTDKIHPNACPALVADLSSGEQGIIALAFGYTRLFQPDKPVTK 644 Query: 833 GQAAIALATGEAADIVSEELARIEAESMAETAVAAHSALVAKVEKDINASFEKELIMERE 654 QAA+ALATG+A++IVSEELARIEAES+AE AVAAHSALVA+VEKDINASFE+EL +ERE Sbjct: 645 AQAAMALATGDASEIVSEELARIEAESVAENAVAAHSALVAQVEKDINASFEQELFIERE 704 Query: 653 KIDAVEKMAQXXXXXXXXXXXXXXXETNSLMRGQVAVESEMQVLSSLRHEVEEQLQSLMS 474 KI AVE+MA+ + +L + + A++SEM+V S LRHEVE+QLQSLM+ Sbjct: 705 KISAVERMAEEARLELERLRAEREEDNLALTKERAAIDSEMEVFSKLRHEVEDQLQSLMN 764 Query: 473 DKMEVSFERERINKLCKEAESENQAITRLQYELEVERKALSMARAWAEDEAKRAREQAKA 294 D++E++ E+ERI+KL ++AE EN+ I RLQYELEVERKALSMARAWAEDEAKR REQA A Sbjct: 765 DRVEIAHEKERISKLREQAEVENKEICRLQYELEVERKALSMARAWAEDEAKRVREQAIA 824 Query: 293 LEEARDRWEKHGIKVIVDDDLREDANAGVTWLTAGTHSTVDATVSRAESLVLKLKTLAGE 114 LEEARDRWE+HGIKV+VDDDLR++A+AGVTWL A +V TV RAESL+ KLK +A + Sbjct: 825 LEEARDRWERHGIKVVVDDDLRKEASAGVTWLNASEQVSVQGTVDRAESLLDKLKQMAAD 884 Query: 113 IRGKSRFVIETIIFKITSLISSLKEWAANSARRAGE 6 IRGKSR ++ II ++ LIS L+EWA + ++A E Sbjct: 885 IRGKSRDTLDKIIHMVSQLISKLREWACKTGKQAEE 920 >ref|XP_002318812.1| predicted protein [Populus trichocarpa] gi|222859485|gb|EEE97032.1| predicted protein [Populus trichocarpa] Length = 793 Score = 607 bits (1564), Expect = e-171 Identities = 351/632 (55%), Positives = 431/632 (68%), Gaps = 14/632 (2%) Frame = -2 Query: 1856 ISMSSGTEIFDISTDLSSGSGGLIPNESDDSVKSDLVAMPSWPVDSEEVVETETALSPQE 1677 + + +G +S S S +P S S K D S +S++ +ET+ + SP+ Sbjct: 100 VILFAGLTFAALSLSKRSTSSKWLPEVSLVSDKEDDKVEESESKESKKGIETDLSSSPE- 158 Query: 1676 DHNLSKMVQVPKQVEGNYLNENGSTETNP---QLIFSDSYTNEPYMNTELGGSNPYFDSL 1506 + + P + N L +N T + SD+ NEP SN S+ Sbjct: 159 ------LNEAPSE---NKLGDNKETSVDSVDYATRVSDTVDNEPVQENLQYESNFDDKSV 209 Query: 1505 VPENS----------ITSSGIPAPSLVSAALQXXXXXXXXXXXXXXXXXXXXXXXXXXXV 1356 PE + I+++GIPAPS VSAALQ V Sbjct: 210 TPETTTSSENLPSSDISATGIPAPSAVSAALQVLPGKVLVPAVVDQVQGQVLAALQVLKV 269 Query: 1355 IEADAHPGDLCTRREYARWLVXXXXXXXXXXXSKVYPAMYIENVTELAFDDITPEDPDFA 1176 IEAD DLCTRRE+ARWLV SKVYPAMYIEN TELAFDDITP+DPDF+ Sbjct: 270 IEADIQSSDLCTRREFARWLVTASSALSRSTVSKVYPAMYIENFTELAFDDITPDDPDFS 329 Query: 1175 FIQGLAEAGLISSKLSRTDMLGSSDEEQDPSLFFPESPLSRQDLVSWKMALDKRWLPDVD 996 IQGLAEAGLISSKLS +L SS E Q P F ESPLSRQDLVSWKMAL+KR P+ D Sbjct: 330 SIQGLAEAGLISSKLSSGGLLSSSVENQGPFYFAAESPLSRQDLVSWKMALEKRQFPEAD 389 Query: 995 KKIVYQSCGFIDIDKINPDAWPALVADQSAGEEGIMALAFGYTRLFQPYKPVTKGQAAIA 816 KK++Y+ GF DIDK+NPDAWPALVAD SAG++GI++LAFG TRLFQP KPVTK QAA+A Sbjct: 390 KKMLYKVSGFRDIDKLNPDAWPALVADLSAGDQGIISLAFGCTRLFQPDKPVTKAQAAVA 449 Query: 815 LATGEAADIVSEELARIEAESMAETAVAAHSALVAKVEKDINASFEKELIMEREKIDAVE 636 LATGEA+DIVSEELARIEAE++AE V+AH+ALVA+VE+D+NASFEKEL +EREKI+A+E Sbjct: 450 LATGEASDIVSEELARIEAEAVAENVVSAHNALVAQVEQDVNASFEKELSIEREKINAIE 509 Query: 635 KMAQXXXXXXXXXXXXXXXETNSLMRGQVAVESEMQVLSSLRHEVEEQLQSLMSDKMEVS 456 KMA+ + +LM+ + A+ESEM+VLS LR E+EEQLQSL+S+K+E+S Sbjct: 510 KMAEEARCELETLRAEREKDDIALMKERAAIESEMEVLSKLRRELEEQLQSLLSNKVEIS 569 Query: 455 FERERINKLCKEAESENQAITRLQYELEVERKALSMARAWAEDEAKRAREQAKALEEARD 276 +E+ERI+KL KEAESE Q I+RLQY+LEVERKALSMARAWAEDEAKRAREQAKALEEAR Sbjct: 570 YEKERISKLQKEAESEKQEISRLQYDLEVERKALSMARAWAEDEAKRAREQAKALEEARY 629 Query: 275 RWEKHGIKVIVDDDLREDANAGVTWLTAGTH-STVDATVSRAESLVLKLKTLAGEIRGKS 99 RWEKHGIKV+VD DL E+++ GVTWLTAG S+V+ TV+RAE+LV +LK +A +IRGKS Sbjct: 630 RWEKHGIKVVVDSDLNEESSTGVTWLTAGKQVSSVEGTVNRAENLVDRLKLMADDIRGKS 689 Query: 98 RFVIETIIFKITSLISSLKEWAANSARRAGEL 3 R V++ II KI LIS LKEW A + R EL Sbjct: 690 RVVLDKIIQKILVLISVLKEWIAEACARTKEL 721 >ref|XP_003549040.1| PREDICTED: uncharacterized protein LOC100810148 [Glycine max] Length = 1002 Score = 606 bits (1562), Expect = e-170 Identities = 373/756 (49%), Positives = 465/756 (61%), Gaps = 22/756 (2%) Frame = -2 Query: 2207 VDDINAGTSITQNGESASSHTDD-TNNTSNQEDKQIRXXXXXXXXXXXXXXXXXXXXXXD 2031 VDD + G+ + + ++ S DD T + S QED Q + Sbjct: 209 VDDSDIGSQLIYDSKNPSDGVDDATKHISVQEDLQ---DVSAFDNKLVFASESPVPLESE 265 Query: 2030 LVSGHANAFSLKD----PEVDPADAVPGLITEPKDNLVPNNLDYPPVSDDASA-YVSPDF 1866 NA+ +D P VD ++ P L K+NL + P DDA +++ + Sbjct: 266 NTVDSFNAYGFRDFDSNPNVDTVESTPNL----KENLFNVDPGDVPNYDDAKPLHLNTEQ 321 Query: 1865 QNEISMSSGTEIFDISTDLSSGSGGLIPNESDDSVKSDLVAMPSWPVDSEEVVETETALS 1686 +EI+ SSG+ F SS V S+L M S P E + E LS Sbjct: 322 HDEITSSSGSVSFGFPETYSSSGADNETGIVSVVVISELNNMISDPKFFNEAGQ-ENILS 380 Query: 1685 PQEDHNL--SKMVQVP----------KQVEGNYLNENGSTETNPQLIFSDSYTNEPY--- 1551 ++ NL +K+ QV + + GN L E S T+ + + N+ Y Sbjct: 381 ALKNENLDLNKIPQVSAEGNEPSFEERSIPGNDLFEKSSISTSANTLVDEQVRNDNYEVD 440 Query: 1550 -MNTELGGSNPYFDSLVPENSITSSGIPAPSLVSAALQXXXXXXXXXXXXXXXXXXXXXX 1374 + +E S +F VP GIPAP +VS A++ Sbjct: 441 EVKSESSNSGSFFS--VP-------GIPAPLVVSTAVKVLPGKILVPAAVDQAQGQALAA 491 Query: 1373 XXXXXVIEADAHPGDLCTRREYARWLVXXXXXXXXXXXSKVYPAMYIENVTELAFDDITP 1194 VIE D P DLCTRREYARWLV SKVYPAMYI+N TELAFDD+TP Sbjct: 492 LQVLKVIEPDVQPSDLCTRREYARWLVSASSALSRSTVSKVYPAMYIDNATELAFDDVTP 551 Query: 1193 EDPDFAFIQGLAEAGLISSKLSRTDMLGSSDEEQDPSLFFPESPLSRQDLVSWKMALDKR 1014 EDPDF+ IQGLAEAGLI S+LSR D+ D + P F PESPLSRQDLVSWKMAL KR Sbjct: 552 EDPDFSSIQGLAEAGLIESRLSRRDIQLFGDGDDSPFYFSPESPLSRQDLVSWKMALQKR 611 Query: 1013 WLPDVDKKIVYQSCGFIDIDKINPDAWPALVADQSAGEEGIMALAFGYTRLFQPYKPVTK 834 LP+ D K++YQ GFID DKI+P+A PALVAD SAGE+GI+ALAFGYTRLFQP KPVTK Sbjct: 612 QLPEADSKVLYQLSGFIDTDKIHPNACPALVADLSAGEQGIIALAFGYTRLFQPDKPVTK 671 Query: 833 GQAAIALATGEAADIVSEELARIEAESMAETAVAAHSALVAKVEKDINASFEKELIMERE 654 QAA+ALATG+A++IVSEELARIEAES+AE AVAAHSALVA+VEKDINASFE+EL +ERE Sbjct: 672 AQAAMALATGDASEIVSEELARIEAESIAENAVAAHSALVAQVEKDINASFEQELFIERE 731 Query: 653 KIDAVEKMAQXXXXXXXXXXXXXXXETNSLMRGQVAVESEMQVLSSLRHEVEEQLQSLMS 474 KI AVE+MA+ + +L + + A+ESEM+V S LRHEVE+QLQSLMS Sbjct: 732 KISAVERMAEEARLELERLRAEREEDNLALTKERAAIESEMEVFSKLRHEVEDQLQSLMS 791 Query: 473 DKMEVSFERERINKLCKEAESENQAITRLQYELEVERKALSMARAWAEDEAKRAREQAKA 294 DK+E++ E+ERI+KL ++AE EN I RLQYELEVERKALSMARAWAEDEAKR REQA A Sbjct: 792 DKVEIAHEKERISKLREKAEVENNEIGRLQYELEVERKALSMARAWAEDEAKRVREQAIA 851 Query: 293 LEEARDRWEKHGIKVIVDDDLREDANAGVTWLTAGTHSTVDATVSRAESLVLKLKTLAGE 114 LEEARDRWE+HGIKV+VDDDLR++A+AGVTWL A +V TV RAESL+ KLK +A + Sbjct: 852 LEEARDRWERHGIKVVVDDDLRKEASAGVTWLNASEQVSVQGTVDRAESLLDKLKQMAAD 911 Query: 113 IRGKSRFVIETIIFKITSLISSLKEWAANSARRAGE 6 IRGKSR + II ++ IS L+EWA + ++A E Sbjct: 912 IRGKSRDTLHKIIHVVSQFISKLREWACKTGKQAEE 947