BLASTX nr result
ID: Coptis25_contig00010039
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00010039
(2023 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002314825.1| predicted protein [Populus trichocarpa] gi|2... 427 e-150
ref|XP_002280212.1| PREDICTED: MATE efflux family protein 5 [Vit... 426 e-149
emb|CAN78227.1| hypothetical protein VITISV_022104 [Vitis vinifera] 424 e-149
ref|XP_002280189.2| PREDICTED: MATE efflux family protein 5 [Vit... 404 e-147
emb|CBI26941.3| unnamed protein product [Vitis vinifera] 426 e-143
>ref|XP_002314825.1| predicted protein [Populus trichocarpa] gi|222863865|gb|EEF00996.1|
predicted protein [Populus trichocarpa]
Length = 482
Score = 427 bits (1097), Expect(3) = e-150
Identities = 205/308 (66%), Positives = 244/308 (79%)
Frame = -3
Query: 1616 LETLCGQAYGAQQYQKLGNYMYXXXXXXXXXXXXXXXIWVNMGKLLTFIGQDPLISLEAG 1437
LETLCGQAYGA+QY+KLG Y IW+ MGK+L F GQDP IS EAG
Sbjct: 94 LETLCGQAYGAKQYKKLGTQTYTAIFCLNLVCIPLSVIWIYMGKILHFTGQDPAISHEAG 153
Query: 1436 KYAAWLIPALFAYATLQSQMRYLQSQSLILPMLLSSFAALCIHIPVCWFLVYKSALRTVG 1257
K+ WL+PALFAYATLQ +RY Q+QS I+PML+SS A LC HIP+CW LVYKS L+ VG
Sbjct: 154 KFIVWLVPALFAYATLQPLVRYFQTQSFIMPMLISSCATLCFHIPLCWALVYKSGLKNVG 213
Query: 1256 AALSIGIAYWLNVIILALYIKYSSSCEKTRVSLSKDALKGIGEFLRLAVPSTIMVCLEWW 1077
+A+++GI+YWLNVI L LYIKYSS+C KTRV + K+ G+GEF R A+PS +M+CLEWW
Sbjct: 214 SAVAMGISYWLNVIFLGLYIKYSSACAKTRVPIYKELFYGVGEFFRFAIPSAVMICLEWW 273
Query: 1076 SFELLILMSGLLPNPQVETSVLSVCLTTISFLYAIPFGVGAAASTRVANELGAGKPKAAR 897
SFELLIL+SG LPNPQ+ETSVLSVCL TIS LY IPFG+GAAASTRVANELGAG P+AAR
Sbjct: 274 SFELLILLSGFLPNPQLETSVLSVCLMTISTLYGIPFGLGAAASTRVANELGAGNPRAAR 333
Query: 896 VAVFVVMLLAATETTIVSSSLFLSRHILGYAYSNEKEVADYVTAMVPLICISVILDSLQG 717
+AV+ M LA +ET IV+S+LF SR + GY +SNEKEV DYVT M PL+C+SVI+DSLQG
Sbjct: 334 LAVYAAMFLAVSETIIVTSALFASRRVFGYLFSNEKEVIDYVTTMAPLVCLSVIMDSLQG 393
Query: 716 TLSGIARG 693
LSG+ARG
Sbjct: 394 VLSGVARG 401
Score = 89.7 bits (221), Expect(3) = e-150
Identities = 42/70 (60%), Positives = 52/70 (74%)
Frame = -2
Query: 588 LGAYVNLGAFYLVGIPIAVLLSFRQHLGGRGLWIGILMGSVVQVTLLSLITGLTNWQQQA 409
+GAY+NLGAFYL GIP A LL+F +LGG GLWIGI G+ Q LLS++T TNW++QA
Sbjct: 407 IGAYINLGAFYLCGIPAAALLAFWLNLGGMGLWIGIQTGAFTQTILLSIVTSCTNWEKQA 466
Query: 408 MKAKERISGG 379
A+ERI G
Sbjct: 467 RMARERIFEG 476
Score = 67.0 bits (162), Expect(3) = e-150
Identities = 34/63 (53%), Positives = 42/63 (66%)
Frame = -1
Query: 1882 TWDVVKEEVKKIGYLAGPMVAVTLSQFLLQLISMMMVGHLGEXXXXXXXXXXXXXXXTGF 1703
TW V +EVK++GY+AGPMVAV L+Q+ LQ+ISMMMVGHLGE TGF
Sbjct: 25 TWGVFIQEVKRLGYIAGPMVAVILTQYSLQVISMMMVGHLGELALSSAAMALSLSGVTGF 84
Query: 1702 SFL 1694
S +
Sbjct: 85 SLM 87
>ref|XP_002280212.1| PREDICTED: MATE efflux family protein 5 [Vitis vinifera]
Length = 481
Score = 426 bits (1096), Expect(3) = e-149
Identities = 204/308 (66%), Positives = 246/308 (79%)
Frame = -3
Query: 1616 LETLCGQAYGAQQYQKLGNYMYXXXXXXXXXXXXXXXIWVNMGKLLTFIGQDPLISLEAG 1437
LETLCGQAYGA+QY KLG Y IW+ MGKLLTF+GQDPLIS EAG
Sbjct: 90 LETLCGQAYGAKQYSKLGTQTYTAIFSLFLVCLPLSVIWIYMGKLLTFMGQDPLISYEAG 149
Query: 1436 KYAAWLIPALFAYATLQSQMRYLQSQSLILPMLLSSFAALCIHIPVCWFLVYKSALRTVG 1257
K+ WL+PALFAYATLQ +RY Q+QSL +PMLLSS AALC H+P+CW LV+KS L +G
Sbjct: 150 KFTMWLVPALFAYATLQPLVRYFQTQSLTMPMLLSSCAALCFHMPLCWVLVFKSGLGNLG 209
Query: 1256 AALSIGIAYWLNVIILALYIKYSSSCEKTRVSLSKDALKGIGEFLRLAVPSTIMVCLEWW 1077
AAL+I I+YWLN I L LY+KYSS+CEKTRV +S + +G+GEF R A+PS +M+CLEWW
Sbjct: 210 AALAISISYWLNAIFLVLYMKYSSACEKTRVPVSMEVFQGMGEFFRFAIPSAVMICLEWW 269
Query: 1076 SFELLILMSGLLPNPQVETSVLSVCLTTISFLYAIPFGVGAAASTRVANELGAGKPKAAR 897
SFELLIL+SGLLPNP++ETSVLSVCL TIS LY IP+G+GAA STRV+NELGAG P+ AR
Sbjct: 270 SFELLILLSGLLPNPELETSVLSVCLNTISTLYTIPYGLGAAGSTRVSNELGAGNPQKAR 329
Query: 896 VAVFVVMLLAATETTIVSSSLFLSRHILGYAYSNEKEVADYVTAMVPLICISVILDSLQG 717
+AV+ +M LA TET +VS++LF SR + GY +SNEKEV DYVT M PL+C+SVILDS+QG
Sbjct: 330 LAVYALMFLAVTETALVSTTLFASRRVFGYLFSNEKEVVDYVTNMSPLVCLSVILDSMQG 389
Query: 716 TLSGIARG 693
LSG+ARG
Sbjct: 390 VLSGVARG 397
Score = 89.7 bits (221), Expect(3) = e-149
Identities = 42/76 (55%), Positives = 57/76 (75%)
Frame = -2
Query: 588 LGAYVNLGAFYLVGIPIAVLLSFRQHLGGRGLWIGILMGSVVQVTLLSLITGLTNWQQQA 409
+GA++NLGAFYL GIP+A L+F L GRGLWIGI G+++Q TLL ++TG TNW++ A
Sbjct: 403 IGAFINLGAFYLCGIPVAATLAFWLQLRGRGLWIGIQTGALLQTTLLCVMTGFTNWEKLA 462
Query: 408 MKAKERISGGILSKEN 361
KA++RI G S E+
Sbjct: 463 TKARQRIFEGRYSVED 478
Score = 63.9 bits (154), Expect(3) = e-149
Identities = 33/64 (51%), Positives = 42/64 (65%)
Frame = -1
Query: 1885 LTWDVVKEEVKKIGYLAGPMVAVTLSQFLLQLISMMMVGHLGEXXXXXXXXXXXXXXXTG 1706
L W +V EEVK++G LA PMVAV LSQ+L+Q++S+MMVGHLGE +G
Sbjct: 20 LRWVLVCEEVKRLGCLAAPMVAVVLSQYLVQVVSVMMVGHLGELALSSTAIAISLSGVSG 79
Query: 1705 FSFL 1694
FS L
Sbjct: 80 FSLL 83
>emb|CAN78227.1| hypothetical protein VITISV_022104 [Vitis vinifera]
Length = 481
Score = 424 bits (1091), Expect(3) = e-149
Identities = 203/308 (65%), Positives = 245/308 (79%)
Frame = -3
Query: 1616 LETLCGQAYGAQQYQKLGNYMYXXXXXXXXXXXXXXXIWVNMGKLLTFIGQDPLISLEAG 1437
LETLCGQAYGA+QY KLG Y IW+ MGKLLTF+GQDPLIS EAG
Sbjct: 90 LETLCGQAYGAKQYSKLGTQTYTAIFSLFLVCLPLSVIWIYMGKLLTFMGQDPLISYEAG 149
Query: 1436 KYAAWLIPALFAYATLQSQMRYLQSQSLILPMLLSSFAALCIHIPVCWFLVYKSALRTVG 1257
K+ WL+PALFAYATLQ +RY Q+QSL +PMLLSS AALC H+P+CW LV+KS L +G
Sbjct: 150 KFTMWLVPALFAYATLQPLVRYFQTQSLTMPMLLSSCAALCFHMPLCWVLVFKSGLGNLG 209
Query: 1256 AALSIGIAYWLNVIILALYIKYSSSCEKTRVSLSKDALKGIGEFLRLAVPSTIMVCLEWW 1077
AAL+I I+YWLN I L LY+KYSS+CEKTRV +S + +G+GEF R A+PS +M+CLEWW
Sbjct: 210 AALAISISYWLNAIFLVLYMKYSSACEKTRVPVSMEVFQGMGEFFRFAIPSAVMICLEWW 269
Query: 1076 SFELLILMSGLLPNPQVETSVLSVCLTTISFLYAIPFGVGAAASTRVANELGAGKPKAAR 897
SFELLIL+SGLLPNP++ETSVLSVCL TIS LY IP+G+GAA STRV+NELGAG P+ AR
Sbjct: 270 SFELLILLSGLLPNPELETSVLSVCLNTISTLYTIPYGLGAAGSTRVSNELGAGNPQKAR 329
Query: 896 VAVFVVMLLAATETTIVSSSLFLSRHILGYAYSNEKEVADYVTAMVPLICISVILDSLQG 717
+AV+ +M LA TET +VS++ F SR + GY +SNEKEV DYVT M PL+C+SVILDS+QG
Sbjct: 330 LAVYALMFLAVTETALVSTTXFASRRVFGYLFSNEKEVVDYVTNMSPLVCLSVILDSMQG 389
Query: 716 TLSGIARG 693
LSG+ARG
Sbjct: 390 VLSGVARG 397
Score = 89.7 bits (221), Expect(3) = e-149
Identities = 42/76 (55%), Positives = 57/76 (75%)
Frame = -2
Query: 588 LGAYVNLGAFYLVGIPIAVLLSFRQHLGGRGLWIGILMGSVVQVTLLSLITGLTNWQQQA 409
+GA++NLGAFYL GIP+A L+F L GRGLWIGI G+++Q TLL ++TG TNW++ A
Sbjct: 403 IGAFINLGAFYLCGIPVAATLAFWLQLRGRGLWIGIQTGALLQTTLLCVMTGFTNWEKLA 462
Query: 408 MKAKERISGGILSKEN 361
KA++RI G S E+
Sbjct: 463 TKARQRIFEGRYSVED 478
Score = 63.9 bits (154), Expect(3) = e-149
Identities = 33/64 (51%), Positives = 42/64 (65%)
Frame = -1
Query: 1885 LTWDVVKEEVKKIGYLAGPMVAVTLSQFLLQLISMMMVGHLGEXXXXXXXXXXXXXXXTG 1706
L W +V EEVK++G LA PMVAV LSQ+L+Q++S+MMVGHLGE +G
Sbjct: 20 LRWVLVCEEVKRLGCLAAPMVAVVLSQYLVQVVSVMMVGHLGELALSSTAIAISLSGVSG 79
Query: 1705 FSFL 1694
FS L
Sbjct: 80 FSLL 83
>ref|XP_002280189.2| PREDICTED: MATE efflux family protein 5 [Vitis vinifera]
Length = 481
Score = 404 bits (1039), Expect(3) = e-147
Identities = 194/308 (62%), Positives = 242/308 (78%)
Frame = -3
Query: 1616 LETLCGQAYGAQQYQKLGNYMYXXXXXXXXXXXXXXXIWVNMGKLLTFIGQDPLISLEAG 1437
LETLCGQAYGA+QY KLG Y IW+ M KLLTFIGQDP+IS EAG
Sbjct: 89 LETLCGQAYGAEQYHKLGTQTYTAIVSLLLVCLPLAVIWLYMSKLLTFIGQDPVISHEAG 148
Query: 1436 KYAAWLIPALFAYATLQSQMRYLQSQSLILPMLLSSFAALCIHIPVCWFLVYKSALRTVG 1257
+++ WL+PALF YATLQ+ +RYLQ+QSLI+P+LL+S A L HIP+CW LV+KS L ++G
Sbjct: 149 QFSIWLVPALFGYATLQALVRYLQTQSLIMPLLLTSCAILGFHIPLCWALVFKSGLGSLG 208
Query: 1256 AALSIGIAYWLNVIILALYIKYSSSCEKTRVSLSKDALKGIGEFLRLAVPSTIMVCLEWW 1077
AL+IGI+YWLNVI L LYIKYS +CEKTRV +S + L GIGEF R A+PS +M+CLEWW
Sbjct: 209 GALAIGISYWLNVIFLGLYIKYSPACEKTRVPVSMEILGGIGEFFRFAIPSAVMICLEWW 268
Query: 1076 SFELLILMSGLLPNPQVETSVLSVCLTTISFLYAIPFGVGAAASTRVANELGAGKPKAAR 897
SFELLIL+SGLLPNP++E SVLSVCL T+S LYAIP G+G+A STRV+NELGAG P+ AR
Sbjct: 269 SFELLILLSGLLPNPELEASVLSVCLNTVSTLYAIPHGLGSAGSTRVSNELGAGNPQKAR 328
Query: 896 VAVFVVMLLAATETTIVSSSLFLSRHILGYAYSNEKEVADYVTAMVPLICISVILDSLQG 717
+AV + LA TE ++S++LF+SR + GY +S E+EV DYVT + P +C+SVILDS+QG
Sbjct: 329 LAVHAAVCLAVTEAIVISTTLFVSRRVFGYVFSEEEEVVDYVTNIAPFLCLSVILDSVQG 388
Query: 716 TLSGIARG 693
TLSG+ RG
Sbjct: 389 TLSGVVRG 396
Score = 99.0 bits (245), Expect(3) = e-147
Identities = 44/76 (57%), Positives = 61/76 (80%)
Frame = -2
Query: 588 LGAYVNLGAFYLVGIPIAVLLSFRQHLGGRGLWIGILMGSVVQVTLLSLITGLTNWQQQA 409
+GA++NLGAFYL GIP+AV+L+F HL G+GLW+G+ GS++Q +LL +ITG TNW++QA
Sbjct: 402 IGAFINLGAFYLCGIPVAVILAFVVHLRGKGLWVGLQTGSLLQTSLLLIITGCTNWEEQA 461
Query: 408 MKAKERISGGILSKEN 361
KA++RI G S EN
Sbjct: 462 TKARQRIFEGTSSVEN 477
Score = 68.2 bits (165), Expect(3) = e-147
Identities = 37/64 (57%), Positives = 43/64 (67%)
Frame = -1
Query: 1885 LTWDVVKEEVKKIGYLAGPMVAVTLSQFLLQLISMMMVGHLGEXXXXXXXXXXXXXXXTG 1706
L W +V EEVK++G LA PMVAV LSQ+LLQ+IS+MMVGHLGE TG
Sbjct: 19 LRWVLVGEEVKRLGCLAAPMVAVILSQYLLQVISLMMVGHLGELALSSTAIAISLSGVTG 78
Query: 1705 FSFL 1694
FSFL
Sbjct: 79 FSFL 82
>emb|CBI26941.3| unnamed protein product [Vitis vinifera]
Length = 443
Score = 426 bits (1096), Expect(3) = e-143
Identities = 204/308 (66%), Positives = 246/308 (79%)
Frame = -3
Query: 1616 LETLCGQAYGAQQYQKLGNYMYXXXXXXXXXXXXXXXIWVNMGKLLTFIGQDPLISLEAG 1437
LETLCGQAYGA+QY KLG Y IW+ MGKLLTF+GQDPLIS EAG
Sbjct: 52 LETLCGQAYGAKQYSKLGTQTYTAIFSLFLVCLPLSVIWIYMGKLLTFMGQDPLISYEAG 111
Query: 1436 KYAAWLIPALFAYATLQSQMRYLQSQSLILPMLLSSFAALCIHIPVCWFLVYKSALRTVG 1257
K+ WL+PALFAYATLQ +RY Q+QSL +PMLLSS AALC H+P+CW LV+KS L +G
Sbjct: 112 KFTMWLVPALFAYATLQPLVRYFQTQSLTMPMLLSSCAALCFHMPLCWVLVFKSGLGNLG 171
Query: 1256 AALSIGIAYWLNVIILALYIKYSSSCEKTRVSLSKDALKGIGEFLRLAVPSTIMVCLEWW 1077
AAL+I I+YWLN I L LY+KYSS+CEKTRV +S + +G+GEF R A+PS +M+CLEWW
Sbjct: 172 AALAISISYWLNAIFLVLYMKYSSACEKTRVPVSMEVFQGMGEFFRFAIPSAVMICLEWW 231
Query: 1076 SFELLILMSGLLPNPQVETSVLSVCLTTISFLYAIPFGVGAAASTRVANELGAGKPKAAR 897
SFELLIL+SGLLPNP++ETSVLSVCL TIS LY IP+G+GAA STRV+NELGAG P+ AR
Sbjct: 232 SFELLILLSGLLPNPELETSVLSVCLNTISTLYTIPYGLGAAGSTRVSNELGAGNPQKAR 291
Query: 896 VAVFVVMLLAATETTIVSSSLFLSRHILGYAYSNEKEVADYVTAMVPLICISVILDSLQG 717
+AV+ +M LA TET +VS++LF SR + GY +SNEKEV DYVT M PL+C+SVILDS+QG
Sbjct: 292 LAVYALMFLAVTETALVSTTLFASRRVFGYLFSNEKEVVDYVTNMSPLVCLSVILDSMQG 351
Query: 716 TLSGIARG 693
LSG+ARG
Sbjct: 352 VLSGVARG 359
Score = 89.7 bits (221), Expect(3) = e-143
Identities = 42/76 (55%), Positives = 57/76 (75%)
Frame = -2
Query: 588 LGAYVNLGAFYLVGIPIAVLLSFRQHLGGRGLWIGILMGSVVQVTLLSLITGLTNWQQQA 409
+GA++NLGAFYL GIP+A L+F L GRGLWIGI G+++Q TLL ++TG TNW++ A
Sbjct: 365 IGAFINLGAFYLCGIPVAATLAFWLQLRGRGLWIGIQTGALLQTTLLCVMTGFTNWEKLA 424
Query: 408 MKAKERISGGILSKEN 361
KA++RI G S E+
Sbjct: 425 TKARQRIFEGRYSVED 440
Score = 42.7 bits (99), Expect(3) = e-143
Identities = 22/45 (48%), Positives = 28/45 (62%)
Frame = -1
Query: 1828 MVAVTLSQFLLQLISMMMVGHLGEXXXXXXXXXXXXXXXTGFSFL 1694
MVAV LSQ+L+Q++S+MMVGHLGE +GFS L
Sbjct: 1 MVAVVLSQYLVQVVSVMMVGHLGELALSSTAIAISLSGVSGFSLL 45