BLASTX nr result
ID: Coptis25_contig00007334
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00007334
(987 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002275826.1| PREDICTED: cytochrome P450 71D10 [Vitis vini... 280 1e-92
emb|CBI15435.3| unnamed protein product [Vitis vinifera] 280 1e-92
ref|XP_003634278.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P... 276 3e-91
ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinif... 261 5e-87
ref|XP_002275714.2| PREDICTED: cytochrome P450 71A1-like [Vitis ... 265 6e-87
>ref|XP_002275826.1| PREDICTED: cytochrome P450 71D10 [Vitis vinifera]
Length = 506
Score = 280 bits (717), Expect(2) = 1e-92
Identities = 127/179 (70%), Positives = 156/179 (87%)
Frame = +1
Query: 268 PGVMKQVQEELQSVIALDRMVEEADLIKLDYLKMVIKESMRLHPVTPLLLPHEFIEDITI 447
P VM+Q+QEEL++VI ++RMVEE DL L YL MV+KE +RLHP PLLLPHE IEDIT+
Sbjct: 324 PRVMRQLQEELENVIGMERMVEEVDLANLVYLDMVLKEGLRLHPAGPLLLPHESIEDITL 383
Query: 448 KGYFIPKSSRVLVNTWAIGRDPNVWSSNAEEFFPERFIGTNIDFQGHDFRFLPFGSGRRK 627
GY+IPK SR+++N WAIGRDPN+WS+N E+FFPERFIG+NIDFQG DF+F+PFGSGRRK
Sbjct: 384 NGYYIPKKSRIIINAWAIGRDPNIWSNNVEDFFPERFIGSNIDFQGKDFQFIPFGSGRRK 443
Query: 628 CPGMQLGLMMVELVLAQLVHCFNLELPDGMSPNDLEMTEKFGLTLPRANHLFAVPTYRL 804
CPGMQLGL+ V LVLAQLVHCF+ +LP+GM P++L+M+E+FGL LPRA HL A+PTYRL
Sbjct: 444 CPGMQLGLINVRLVLAQLVHCFDWKLPNGMLPSELDMSEEFGLALPRATHLHALPTYRL 502
Score = 87.0 bits (214), Expect(2) = 1e-92
Identities = 42/91 (46%), Positives = 64/91 (70%), Gaps = 4/91 (4%)
Frame = +2
Query: 8 RRMKAINMVLDGVLEKIIDDYVQDAKELHGRHRDFIDVMLSLMESNNRWELH----LGRD 175
RRMKAI+ +D +LEK+ID++ QDA E G H+DF+DVMLSLM + + ++
Sbjct: 233 RRMKAISGAVDHILEKVIDEHKQDASENQGNHKDFVDVMLSLMNEMKNFHQEPSYLIEQE 292
Query: 176 YIKAIMLDMLGASMDTSSTTIEWVITELLKH 268
IK I+ D++ ++DTS+TTIEW+++EL +H
Sbjct: 293 NIKGIVWDIIIGAIDTSATTIEWLLSELFRH 323
>emb|CBI15435.3| unnamed protein product [Vitis vinifera]
Length = 439
Score = 280 bits (717), Expect(2) = 1e-92
Identities = 127/179 (70%), Positives = 156/179 (87%)
Frame = +1
Query: 268 PGVMKQVQEELQSVIALDRMVEEADLIKLDYLKMVIKESMRLHPVTPLLLPHEFIEDITI 447
P VM+Q+QEEL++VI ++RMVEE DL L YL MV+KE +RLHP PLLLPHE IEDIT+
Sbjct: 177 PRVMRQLQEELENVIGMERMVEEVDLANLVYLDMVLKEGLRLHPAGPLLLPHESIEDITL 236
Query: 448 KGYFIPKSSRVLVNTWAIGRDPNVWSSNAEEFFPERFIGTNIDFQGHDFRFLPFGSGRRK 627
GY+IPK SR+++N WAIGRDPN+WS+N E+FFPERFIG+NIDFQG DF+F+PFGSGRRK
Sbjct: 237 NGYYIPKKSRIIINAWAIGRDPNIWSNNVEDFFPERFIGSNIDFQGKDFQFIPFGSGRRK 296
Query: 628 CPGMQLGLMMVELVLAQLVHCFNLELPDGMSPNDLEMTEKFGLTLPRANHLFAVPTYRL 804
CPGMQLGL+ V LVLAQLVHCF+ +LP+GM P++L+M+E+FGL LPRA HL A+PTYRL
Sbjct: 297 CPGMQLGLINVRLVLAQLVHCFDWKLPNGMLPSELDMSEEFGLALPRATHLHALPTYRL 355
Score = 87.0 bits (214), Expect(2) = 1e-92
Identities = 42/91 (46%), Positives = 64/91 (70%), Gaps = 4/91 (4%)
Frame = +2
Query: 8 RRMKAINMVLDGVLEKIIDDYVQDAKELHGRHRDFIDVMLSLMESNNRWELH----LGRD 175
RRMKAI+ +D +LEK+ID++ QDA E G H+DF+DVMLSLM + + ++
Sbjct: 86 RRMKAISGAVDHILEKVIDEHKQDASENQGNHKDFVDVMLSLMNEMKNFHQEPSYLIEQE 145
Query: 176 YIKAIMLDMLGASMDTSSTTIEWVITELLKH 268
IK I+ D++ ++DTS+TTIEW+++EL +H
Sbjct: 146 NIKGIVWDIIIGAIDTSATTIEWLLSELFRH 176
>ref|XP_003634278.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 71D10-like [Vitis
vinifera]
Length = 506
Score = 276 bits (706), Expect(2) = 3e-91
Identities = 124/179 (69%), Positives = 155/179 (86%)
Frame = +1
Query: 268 PGVMKQVQEELQSVIALDRMVEEADLIKLDYLKMVIKESMRLHPVTPLLLPHEFIEDITI 447
P VM+Q+QEEL++VI ++RMVEE DL L YL MV+KE +RLHP PLLLPHE IEDIT+
Sbjct: 324 PRVMRQLQEELENVIGMERMVEEVDLANLVYLYMVLKEGLRLHPAGPLLLPHESIEDITL 383
Query: 448 KGYFIPKSSRVLVNTWAIGRDPNVWSSNAEEFFPERFIGTNIDFQGHDFRFLPFGSGRRK 627
GY+IPK S++++N WAIGRDPN+WS+N E+FFPERFIG+NIDFQG DF+F+PFGSGRRK
Sbjct: 384 NGYYIPKKSQIIINAWAIGRDPNIWSNNVEDFFPERFIGSNIDFQGKDFQFIPFGSGRRK 443
Query: 628 CPGMQLGLMMVELVLAQLVHCFNLELPDGMSPNDLEMTEKFGLTLPRANHLFAVPTYRL 804
CPGM LGL+ + LVLAQLVHCF+ +LP+GM P++L+M+E+FGL LPRA HL A+PTYRL
Sbjct: 444 CPGMHLGLINIRLVLAQLVHCFDWKLPNGMLPSELDMSEEFGLALPRATHLHALPTYRL 502
Score = 86.3 bits (212), Expect(2) = 3e-91
Identities = 42/91 (46%), Positives = 62/91 (68%), Gaps = 4/91 (4%)
Frame = +2
Query: 8 RRMKAINMVLDGVLEKIIDDYVQDAKELHGRHRDFIDVMLSLMESNNRWELH----LGRD 175
RRMKAI+ +D +LEKIID++ QDA E G H DF+DVMLSLM + + +
Sbjct: 233 RRMKAISGAVDHILEKIIDEHKQDASENQGNHNDFVDVMLSLMNETKNFHQEPSYLIKXE 292
Query: 176 YIKAIMLDMLGASMDTSSTTIEWVITELLKH 268
+K I+ D++ ++DTS+TTIEW+++EL +H
Sbjct: 293 NLKGIVWDIIVGAIDTSATTIEWLLSELFRH 323
>ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinifera]
Length = 498
Score = 261 bits (667), Expect(2) = 5e-87
Identities = 120/181 (66%), Positives = 155/181 (85%)
Frame = +1
Query: 262 KAPGVMKQVQEELQSVIALDRMVEEADLIKLDYLKMVIKESMRLHPVTPLLLPHEFIEDI 441
K P +MK+VQEEL+ V+ ++R VEE+DL L+YL MV+KE++RLHPV PLL+PHE +ED
Sbjct: 317 KNPRIMKKVQEELEKVVGMERKVEESDLESLEYLDMVVKETLRLHPVAPLLIPHESLEDC 376
Query: 442 TIKGYFIPKSSRVLVNTWAIGRDPNVWSSNAEEFFPERFIGTNIDFQGHDFRFLPFGSGR 621
T+ G+ IP+ SRV+VNT+AIGRDPNVW+ +AE+F PERFIG++ID +G DF+ +PFGSGR
Sbjct: 377 TVNGFHIPQKSRVMVNTYAIGRDPNVWT-DAEKFLPERFIGSSIDLRGRDFQLIPFGSGR 435
Query: 622 RKCPGMQLGLMMVELVLAQLVHCFNLELPDGMSPNDLEMTEKFGLTLPRANHLFAVPTYR 801
R CPGMQLGL +V LVLAQLVHCF+ ELP+GM P++L+MTE+FGLT+PRA H+ AVPTYR
Sbjct: 436 RGCPGMQLGLTVVRLVLAQLVHCFDWELPNGMMPSELDMTEEFGLTVPRAKHILAVPTYR 495
Query: 802 L 804
L
Sbjct: 496 L 496
Score = 87.4 bits (215), Expect(2) = 5e-87
Identities = 47/87 (54%), Positives = 59/87 (67%)
Frame = +2
Query: 8 RRMKAINMVLDGVLEKIIDDYVQDAKELHGRHRDFIDVMLSLMESNNRWELHLGRDYIKA 187
RRMKAI+ V D EKIIDD++ + KE G+ +D IDVML M S E + R IKA
Sbjct: 234 RRMKAISKVFDAFFEKIIDDHIHEPKE-EGQPKDLIDVMLGYMGSKEN-EFQIERSNIKA 291
Query: 188 IMLDMLGASMDTSSTTIEWVITELLKH 268
++LDML SMDTS+T IEW + ELLK+
Sbjct: 292 LVLDMLAGSMDTSATAIEWALAELLKN 318
>ref|XP_002275714.2| PREDICTED: cytochrome P450 71A1-like [Vitis vinifera]
Length = 502
Score = 265 bits (676), Expect(2) = 6e-87
Identities = 120/181 (66%), Positives = 148/181 (81%)
Frame = +1
Query: 268 PGVMKQVQEELQSVIALDRMVEEADLIKLDYLKMVIKESMRLHPVTPLLLPHEFIEDITI 447
P VM+Q+Q ELQ+V+ +DRMV+E+DL L YL MV+KE +RLHP+ P L+PH EDITI
Sbjct: 322 PRVMRQLQHELQNVVKMDRMVDESDLENLVYLNMVVKEVLRLHPIGPFLVPHASTEDITI 381
Query: 448 KGYFIPKSSRVLVNTWAIGRDPNVWSSNAEEFFPERFIGTNIDFQGHDFRFLPFGSGRRK 627
+G+FIPK S +L+NTWAIGRDPN WS N +EF PERFI +NID QG DF +PFGSGRR
Sbjct: 382 EGHFIPKRSTILINTWAIGRDPNFWSDNVDEFLPERFINSNIDLQGRDFELIPFGSGRRG 441
Query: 628 CPGMQLGLMMVELVLAQLVHCFNLELPDGMSPNDLEMTEKFGLTLPRANHLFAVPTYRLY 807
CPG+QLGL V LVLAQL+HCFN ELP+ MS +DL+M+EKFGLT+PR NHL+A+PTYRL
Sbjct: 442 CPGIQLGLRTVRLVLAQLLHCFNWELPNDMSSDDLDMSEKFGLTMPRVNHLYAIPTYRLL 501
Query: 808 V 810
+
Sbjct: 502 I 502
Score = 83.6 bits (205), Expect(2) = 6e-87
Identities = 50/91 (54%), Positives = 62/91 (68%), Gaps = 4/91 (4%)
Frame = +2
Query: 8 RRMKAINMVLDGVLEKIIDDYVQDAKELHGRHRDFIDVMLSLME--SNNRWELH--LGRD 175
+R KAIN D VLEKIID +QD + H H +FID+MLSLM SN R E + R
Sbjct: 232 QRFKAINKAADEVLEKIIDRRIQDGGKDHN-HSNFIDIMLSLMSNFSNLRSESSYIIDRT 290
Query: 176 YIKAIMLDMLGASMDTSSTTIEWVITELLKH 268
+KAI+LDML +D+SSTTIEWV +ELL+H
Sbjct: 291 NVKAILLDMLVGGIDSSSTTIEWVFSELLRH 321