BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00004963
         (1805 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002877272.1| pectinesterase family protein [Arabidopsis l...   654   0.0  
gb|AER38245.1| PME4 [Gossypium barbadense]                            653   0.0  
ref|XP_004146188.1| PREDICTED: pectinesterase/pectinesterase inh...   649   0.0  
dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis]              649   0.0  
gb|AER38246.1| PME3 [Gossypium barbadense]                            647   0.0  

>ref|XP_002877272.1| pectinesterase family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297323110|gb|EFH53531.1| pectinesterase family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 527

 Score =  654 bits (1688), Expect = 0.0
 Identities = 320/523 (61%), Positives = 391/523 (74%), Gaps = 21/523 (4%)
 Frame = +1

Query: 64   LMSNFSVMIFFFFTLSPFFVKSDYYQ-----RAELLKVPSATFLTSVKSTIDEVKVATSI 228
            L    S+  FF  T+S      + +      + E LKVP   F  + K+ +D ++ A +I
Sbjct: 5    LQMGSSIFFFFLITVSLCSAHKEAFSSTGLVQMECLKVPPLEFAQAAKTVVDAIQKAVAI 64

Query: 229  VSKFVNKFVNSRLLNAINDCVDLLDYTADELSLTLSAIENPNGNDNSTGDRGVDLKAWLS 408
            VSKF  K   SR+ NAI DCVDLLD  A+ELS  +SA +NPNG DNSTGD G DL+ W+S
Sbjct: 65   VSKFDKKVGKSRVSNAILDCVDLLDSAAEELSWIISASQNPNGKDNSTGDVGSDLRTWIS 124

Query: 409  AALGNQETCLDGFEGTNSFVKKVIGGSLQKVTSRVANVLGMVGQVPSNKNSPRD------ 570
            AAL NQ+TCLDGFEGTN  +KK++ G L +V + V N+L MV   PS K  P+       
Sbjct: 125  AALSNQDTCLDGFEGTNGIIKKIVAGGLSRVGTTVRNLLTMVHSPPS-KAKPKPIKAHTM 183

Query: 571  ---------FPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSVMDAILAAPDYSTRR 723
                     FP+W+KPGDR+LLQ              DG+G+F+++ DA+LAAPDYST+R
Sbjct: 184  TKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVAT-DGTGNFTTISDAVLAAPDYSTKR 242

Query: 724  YVIYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWTTFRSATFAVSAKGF 903
            YVI+VKRGVY+ENV+IKKKKWN+M++GDG+DAT+I+GNR+F+DGWTTFRSATFAVS +GF
Sbjct: 243  YVIHVKRGVYEENVEIKKKKWNIMIVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGF 302

Query: 904  IARDISIENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHSLRQFYRECRITGTI 1083
            I RDI+ +NTAGPEKHQAVA+RSD+DL  FYRC  RGYQDTLYAHS+RQF+REC ITGT+
Sbjct: 303  IGRDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTV 362

Query: 1084 DYIFGNAAAVFQNCQILAKKGLPNQKNTITAQGRKDPNQSTGFSIQFCNISADSDLLPFV 1263
            D+IFG+A AVFQNCQI AK+GLPNQKN+ITAQGRKDPN+ TGF+IQF NI+AD+DLLP  
Sbjct: 363  DFIFGDATAVFQNCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLPNS 422

Query: 1264 NTTQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTLYYGEYMNYGPGTGLG 1443
            NTT TYLGRPWK YSRTVFMQ+YMS  + P GWLEW G+FAL TLYYGEYMN GPG  L 
Sbjct: 423  NTTATYLGRPWKLYSRTVFMQNYMSDAINPEGWLEWNGNFALDTLYYGEYMNSGPGASLD 482

Query: 1444 SRVKWPGYHQIDS-TQAATFTVTQFIDGNLWLPPTGVKFTAGL 1569
             RVKWPGYH +++  +A  FTV+QFI GNLWLP TG+ F AGL
Sbjct: 483  RRVKWPGYHVLNTPAEANNFTVSQFIQGNLWLPSTGITFIAGL 525


>gb|AER38245.1| PME4 [Gossypium barbadense]
          Length = 525

 Score =  653 bits (1685), Expect = 0.0
 Identities = 323/523 (61%), Positives = 393/523 (75%), Gaps = 23/523 (4%)
 Frame = +1

Query: 76   FSVMIFFFFTL-----SPFFVKSDYYQRAELLKVPSATFLTSVKSTIDEVKVATSIVSKF 240
            F +++FF   L     S     ++ +   E LKVP+  F+ S+K+TID ++ ATS+VS+F
Sbjct: 5    FHLLVFFLVALCLCTNSNASSSTNEFLETECLKVPATEFIGSLKTTIDAIRKATSVVSQF 64

Query: 241  VNKFVNSRLLNAINDCVDLLDYTADELSLTLSAIENPNGNDNSTGDRGVDLKAWLSAALG 420
               F + RL NAI+DC+DLLD +ADELS T+SA +NPN  DNSTGD   DL+ WLSAA+ 
Sbjct: 65   GGFFHDFRLSNAISDCLDLLDSSADELSWTMSASQNPNAKDNSTGDLSSDLRTWLSAAMV 124

Query: 421  NQETCLDGFEGTNSFVKKVIGGSLQKVTSRVANVLGMVGQVPSNK-NSPRD--------- 570
            NQ+TC+DGFEGTNS VK V+ GSL ++TS V N+L MV   P++K N  R+         
Sbjct: 125  NQQTCIDGFEGTNSMVKTVVSGSLNQITSLVRNLLIMVHPGPNSKSNGTRNGSQKGGGGG 184

Query: 571  -------FPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSVMDAILAAPDYSTRRYV 729
                   FP W K  DRRLLQ              DGSG+F+ +MDA+  APD S  RYV
Sbjct: 185  GHPGQSRFPVWFKREDRRLLQI--NGVTANVVVAADGSGNFTRIMDAVETAPDKSMNRYV 242

Query: 730  IYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWTTFRSATFAVSAKGFIA 909
            IY+K+G+YKENV+IKKKKWNL+MIGDGMD T+ISGNR+F+DGWTT RSATFAVS +GFIA
Sbjct: 243  IYIKKGLYKENVEIKKKKWNLVMIGDGMDVTVISGNRSFIDGWTTLRSATFAVSGRGFIA 302

Query: 910  RDISIENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHSLRQFYRECRITGTIDY 1089
            RDI+ ENTAGP+KHQAVA+RSDSDLS F+RC  +GYQD+LY H++RQFYREC+ITGT+D+
Sbjct: 303  RDITFENTAGPQKHQAVALRSDSDLSVFFRCAIKGYQDSLYTHTMRQFYRECKITGTVDF 362

Query: 1090 IFGNAAAVFQNCQILAKKGLPNQKNTITAQGRKDPNQSTGFSIQFCNISADSDLLPFVNT 1269
            IFG+ A +FQNCQILAK+GLP+QKNTITAQGRKDPNQ TGFSIQFCNISAD+DLLP VN+
Sbjct: 363  IFGDGAVLFQNCQILAKQGLPSQKNTITAQGRKDPNQPTGFSIQFCNISADTDLLPSVNS 422

Query: 1270 TQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTLYYGEYMNYGPGTGLGSR 1449
            T TYLGRPWK YSRT+ MQSY+S  + P GWLEW  DFAL TLYY EYMN GPG  L  R
Sbjct: 423  TPTYLGRPWKLYSRTIIMQSYISDAIRPQGWLEWNQDFALDTLYYAEYMNNGPGASLSER 482

Query: 1450 VKWPGYHQI-DSTQAATFTVTQFIDGNLWLPPTGVKFTAGLTV 1575
            VKWPGYH + +S QA  FTV QFI+G+LWLP TGVK+T+G  V
Sbjct: 483  VKWPGYHVLNNSAQAVNFTVAQFIEGDLWLPSTGVKYTSGFGV 525


>ref|XP_004146188.1| PREDICTED: pectinesterase/pectinesterase inhibitor PPE8B-like
            [Cucumis sativus]
          Length = 507

 Score =  649 bits (1674), Expect = 0.0
 Identities = 317/497 (63%), Positives = 384/497 (77%), Gaps = 1/497 (0%)
 Frame = +1

Query: 82   VMIFFFFTLSPFFVKSDYYQRAELLKVPSATFLTSVKSTIDEVKVATSIVSKFVNKFVNS 261
            ++I  F  L P     D  Q  E L VPS+ F  S+ STID V+   +I S F     + 
Sbjct: 14   LLILLFPMLLPASSPPDLVQ-TECLSVPSSQFSNSLLSTIDVVRQVMAIFSPFSKLLGDF 72

Query: 262  RLLNAINDCVDLLDYTADELSLTLSAIENPNGNDNSTGDRGVDLKAWLSAALGNQETCLD 441
            RL  AI+DC+DLLD +AD+LS +LSA +NP   ++STGD   DLK WLSAA+ N ETC+D
Sbjct: 73   RLSTAISDCLDLLDSSADQLSWSLSATQNPKAKNHSTGDLSSDLKTWLSAAVVNPETCMD 132

Query: 442  GFEGTNSFVKKVIGGSLQKVTSRVANVLGMVGQVPSNKNSPRDFPAWMKPGDRRLLQAPT 621
            GFEGTNS +K ++ G + ++TS++ ++L MV  +P   N P +FP+W+K  D+ LLQ   
Sbjct: 133  GFEGTNSIIKGLVSGGVNQLTSQLYDLLSMVKSIP---NQPSEFPSWLKSEDQNLLQI-- 187

Query: 622  XXXXXXXXXXMDGSGSFSSVMDAILAAPDYSTRRYVIYVKRGVYKENVDIKKKKWNLMMI 801
                       DG+G F++VMDA+LAAPD S RRYVIY+K+GVY ENV+IKKKKWNLMMI
Sbjct: 188  NDLAADATVAADGTGDFTNVMDAVLAAPDNSIRRYVIYIKKGVYLENVEIKKKKWNLMMI 247

Query: 802  GDGMDATIISGNRNFVDGWTTFRSATFAVSAKGFIARDISIENTAGPEKHQAVAVRSDSD 981
            GDG+DATIISGNR+F+DGWTTFRSATFAVS +GFIARDI+ ENTAG EKHQAVA+RSDSD
Sbjct: 248  GDGIDATIISGNRSFIDGWTTFRSATFAVSGRGFIARDITFENTAGAEKHQAVALRSDSD 307

Query: 982  LSAFYRCGFRGYQDTLYAHSLRQFYRECRITGTIDYIFGNAAAVFQNCQILAKKGLPNQK 1161
            LS F+RC  RGYQDTLY H++RQFYREC+I+GT+D++FG+A  VFQNC ILAKKGLPNQK
Sbjct: 308  LSVFFRCRIRGYQDTLYTHTMRQFYRECQISGTVDFLFGDATVVFQNCSILAKKGLPNQK 367

Query: 1162 NTITAQGRKDPNQSTGFSIQFCNISADSDLLPFVNTTQTYLGRPWKQYSRTVFMQSYMSS 1341
            NTITAQGRKDPNQ TGFSIQFCNISADSDL P VNTT TYLGRPWK+YSRT+ MQSY+S 
Sbjct: 368  NTITAQGRKDPNQPTGFSIQFCNISADSDLKPSVNTTATYLGRPWKEYSRTIIMQSYISD 427

Query: 1342 VLMPAGWLEWQGDFALSTLYYGEYMNYGPGTGLGSRVKWPGYHQIDSTQAAT-FTVTQFI 1518
             + P GWLEW  +FAL+TL+Y E+MNYGPG GL  RV WPGYH+++ T  AT FTV QFI
Sbjct: 428  AIRPEGWLEWNANFALNTLFYAEFMNYGPGAGLAKRVNWPGYHRLNQTSEATNFTVAQFI 487

Query: 1519 DGNLWLPPTGVKFTAGL 1569
            +GNLWLP TGVK+TAGL
Sbjct: 488  EGNLWLPSTGVKYTAGL 504


>dbj|BAF42041.1| pectin methylesterase 4 [Pyrus communis]
          Length = 509

 Score =  649 bits (1673), Expect = 0.0
 Identities = 315/477 (66%), Positives = 377/477 (79%), Gaps = 1/477 (0%)
 Frame = +1

Query: 142  RAELLKVPSATFLTSVKSTIDEVKVATSIVSKFVNKFVNSRLLNAINDCVDLLDYTADEL 321
            R+E LKVP++ F  S++ TI  V+   SI+S+F   F +  L NAI+DC+DLLD+TADEL
Sbjct: 33   RSECLKVPASEFAGSLRDTIGAVQQVASILSQFATAFGDFHLSNAISDCLDLLDFTADEL 92

Query: 322  SLTLSAIENPNGNDNSTGDRGVDLKAWLSAALGNQETCLDGFEGTNSFVKKVIGGSLQKV 501
            + +LSA +NP G DNSTG    DL+ WLSAAL NQ+TC DGFEGTNS VK ++   L +V
Sbjct: 93   NWSLSASQNPEGKDNSTGKLSSDLRTWLSAALVNQDTCSDGFEGTNSIVKGLVTTGLNQV 152

Query: 502  TSRVANVLGMVGQVPSNKNSPRDFPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGSFSSV 681
            TS V  +L  V     +     + P+W+K  DR+LLQA             DG+G+F++V
Sbjct: 153  TSLVQGLLTQVQPNTDHHGPNGEIPSWVKAEDRKLLQA--GGVNVDAVVAQDGTGNFTNV 210

Query: 682  MDAILAAPDYSTRRYVIYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFVDGWT 861
             DA+LAAPD S  RYVIY+K G YKENV+IKKKKWNLMMIGDGMDATIISG+RNFVDGWT
Sbjct: 211  TDAVLAAPDESMTRYVIYIKGGTYKENVEIKKKKWNLMMIGDGMDATIISGSRNFVDGWT 270

Query: 862  TFRSATFAVSAKGFIARDISIENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTLYAHS 1041
            TFRSATFAVS +GFIARD++ ENTAGPEKHQAVA+RSDSDLS FYRC  RGYQDTLYAH+
Sbjct: 271  TFRSATFAVSGRGFIARDLTFENTAGPEKHQAVALRSDSDLSVFYRCQIRGYQDTLYAHA 330

Query: 1042 LRQFYRECRITGTIDYIFGNAAAVFQNCQILAKKGLPNQKNTITAQGRKDPNQSTGFSIQ 1221
            +RQFYR+C+I+GT+D+IFG+A  VFQNCQILAKKGLPNQKN++TAQGRKDPN+ TG SIQ
Sbjct: 331  MRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSVTAQGRKDPNEPTGISIQ 390

Query: 1222 FCNISADSDLLPFVNTTQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFALSTLY 1401
            FCNI+AD+DLLPFVN+T TYLGRPWK YSRTV MQS++S+ + P GWLEW  DFALSTL 
Sbjct: 391  FCNITADADLLPFVNSTSTYLGRPWKLYSRTVIMQSFLSNAIRPEGWLEWNADFALSTLS 450

Query: 1402 YGEYMNYGPGTGLGSRVKWPGYHQI-DSTQAATFTVTQFIDGNLWLPPTGVKFTAGL 1569
            YGEY+NYGPG GL SRVKWPGY    +STQ   +TV QFI+G+LWLP TGVK+TAGL
Sbjct: 451  YGEYINYGPGAGLESRVKWPGYRVFNESTQVQNYTVAQFIEGDLWLPTTGVKYTAGL 507


>gb|AER38246.1| PME3 [Gossypium barbadense]
          Length = 514

 Score =  647 bits (1668), Expect = 0.0
 Identities = 308/481 (64%), Positives = 384/481 (79%), Gaps = 9/481 (1%)
 Frame = +1

Query: 154  LKVPSATFLTSVKSTIDEVKVATSIVSKFVNKFVNSRLLNAINDCVDLLDYTADELSLTL 333
            + VP A F  S+++TID ++    IVS+F + F + RL NAI+DC+DLLD++AD+LS +L
Sbjct: 34   VNVPPAEFAGSLRTTIDAIRKVIPIVSQFGSFFGDFRLSNAISDCLDLLDFSADQLSWSL 93

Query: 334  SAIENPNGNDNSTGDRGVDLKAWLSAALGNQETCLDGFEGTNSFVKKVIGGSLQKVTSRV 513
            SA +NPNG  NSTGD   DL+ WLSAA+ NQETC++GFEGTN   K V+ G L +VTS V
Sbjct: 94   SASQNPNGKHNSTGDVASDLRTWLSAAMANQETCIEGFEGTNGIAKTVVAGGLNQVTSLV 153

Query: 514  ANVLGMVGQVPSNKNSPRD--------FPAWMKPGDRRLLQAPTXXXXXXXXXXMDGSGS 669
            +++L MV    S+  S  D        FP+W +  D++LLQA            +DG+G+
Sbjct: 154  SDLLTMVQPPGSDSRSNGDRKVAEKNRFPSWFEREDQKLLQA--NGVTADAVVALDGTGT 211

Query: 670  FSSVMDAILAAPDYSTRRYVIYVKRGVYKENVDIKKKKWNLMMIGDGMDATIISGNRNFV 849
            F+++MDA+ AAPDYS  R+VIY+K+G+YKENV+IKKKKWNLMM+GDG++ TIISGNR+FV
Sbjct: 212  FTNIMDAVAAAPDYSMNRHVIYIKKGLYKENVEIKKKKWNLMMVGDGINGTIISGNRSFV 271

Query: 850  DGWTTFRSATFAVSAKGFIARDISIENTAGPEKHQAVAVRSDSDLSAFYRCGFRGYQDTL 1029
            DGWTTFRSATFAVS +GFIARDI+ ENTAGP+KHQAVA+RSDSDLS F+RC  +GYQD+L
Sbjct: 272  DGWTTFRSATFAVSGRGFIARDITFENTAGPQKHQAVALRSDSDLSVFFRCAIKGYQDSL 331

Query: 1030 YAHSLRQFYRECRITGTIDYIFGNAAAVFQNCQILAKKGLPNQKNTITAQGRKDPNQSTG 1209
            Y H++RQF+RECRITGT+D+IFG+A+ VFQNCQILAK+GLPNQKNTITAQGRKDPNQ TG
Sbjct: 332  YTHTMRQFFRECRITGTVDFIFGDASVVFQNCQILAKQGLPNQKNTITAQGRKDPNQPTG 391

Query: 1210 FSIQFCNISADSDLLPFVNTTQTYLGRPWKQYSRTVFMQSYMSSVLMPAGWLEWQGDFAL 1389
            FSIQFCNISAD+DLLPFVN+T TYLGRPWK YSRT+ MQSY+ + + P GWLEW  DFAL
Sbjct: 392  FSIQFCNISADADLLPFVNSTPTYLGRPWKLYSRTIIMQSYIGNAVRPQGWLEWNQDFAL 451

Query: 1390 STLYYGEYMNYGPGTGLGSRVKWPGYHQI-DSTQAATFTVTQFIDGNLWLPPTGVKFTAG 1566
             TLYY E+MN+GPG GLG RV+WPGYH + +S QA  FTV + I+G+LWLP TGVK+TAG
Sbjct: 452  DTLYYAEFMNFGPGAGLGGRVQWPGYHALNNSAQAGNFTVARLIEGDLWLPSTGVKYTAG 511

Query: 1567 L 1569
            L
Sbjct: 512  L 512