BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00004745
         (1678 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 cataly...   222   e-148
ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 cataly...   213   e-148
ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [R...   214   e-143
ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit...   214   e-142
ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|2...   213   e-142

>ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit-like [Cucumis
            sativus] gi|449515377|ref|XP_004164726.1| PREDICTED:
            NEDD8-activating enzyme E1 catalytic subunit-like
            [Cucumis sativus]
          Length = 449

 Score =  222 bits (565), Expect(4) = e-148
 Identities = 103/134 (76%), Positives = 124/134 (92%)
 Frame = +1

Query: 925  YNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQ 1104
            YNG EGLHTK+TEFV+DKDCLVCGPG+L+EL++SITLQKF++L+ +HPKLLLSK SVT++
Sbjct: 316  YNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDHPKLLLSKASVTHR 375

Query: 1105 GKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDKKTSCLRKLRIVFK 1284
            GK LYMQAPPVLEEMTRSNL +PLFDLM K+ KDV+HVTG+ +K+DKKTSCLRKLR+ FK
Sbjct: 376  GKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDKKTSCLRKLRVAFK 435

Query: 1285 GVDGITDVDMASGA 1326
            GVDG+TD+D ASGA
Sbjct: 436  GVDGVTDMDTASGA 449



 Score =  171 bits (434), Expect(4) = e-148
 Identities = 76/81 (93%), Positives = 79/81 (97%)
 Frame = +1

Query: 415 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDADDPEHMQWIY 594
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+FD DD EHM+W+Y
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFDPDDSEHMKWVY 253

Query: 595 SEALKRAELFGIPGVTYSLTQ 657
           SEALKRAELFGIPGVTYSLTQ
Sbjct: 254 SEALKRAELFGIPGVTYSLTQ 274



 Score =  154 bits (389), Expect(4) = e-148
 Identities = 79/113 (69%), Positives = 85/113 (75%)
 Frame = +3

Query: 3   MERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSIEARXXXXXXXXXXXXXXXXXXXX 182
           MERVSGVNI+PHFCRIEDK+++FY +F+IIALGLDSIEAR                    
Sbjct: 106 MERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDSIEAR-------------------- 145

Query: 183 XXXXXSYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTP 341
                SYINSVACSFLEYDSDD P EETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 146 -----SYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP 193



 Score = 50.1 bits (118), Expect(4) = e-148
 Identities = 25/48 (52%), Positives = 28/48 (58%)
 Frame = +3

Query: 720 GVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNGLEGL 863
           GVVK                CALE LKIV+ CS+ +SNYLTYNG EGL
Sbjct: 275 GVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGL 322


>ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Vitis
            vinifera] gi|297736997|emb|CBI26198.3| unnamed protein
            product [Vitis vinifera]
          Length = 449

 Score =  213 bits (543), Expect(4) = e-148
 Identities = 98/134 (73%), Positives = 118/134 (88%)
 Frame = +1

Query: 925  YNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQ 1104
            YNG EGLHTK+TEFV+D DCL+CGPG+L+EL+TS+TLQKFM+ + EHPKLLLSK SV ++
Sbjct: 316  YNGAEGLHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQEHPKLLLSKASVKHR 375

Query: 1105 GKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDKKTSCLRKLRIVFK 1284
             K LY+QAPPVLEE+TRSNL LPLFDLM K+ KD++HVTG T K+DKKTSCLRKLR+VFK
Sbjct: 376  EKNLYIQAPPVLEELTRSNLNLPLFDLMGKIRKDIVHVTGSTTKNDKKTSCLRKLRVVFK 435

Query: 1285 GVDGITDVDMASGA 1326
            G+DG TD+D A GA
Sbjct: 436  GIDGATDMDTAGGA 449



 Score =  174 bits (442), Expect(4) = e-148
 Identities = 77/81 (95%), Positives = 80/81 (98%)
 Frame = +1

Query: 415 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDADDPEHMQWIY 594
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD DDPEHM+W+Y
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDPDDPEHMKWVY 253

Query: 595 SEALKRAELFGIPGVTYSLTQ 657
           SEA+KRAELFGIPGVTYSLTQ
Sbjct: 254 SEAVKRAELFGIPGVTYSLTQ 274



 Score =  158 bits (399), Expect(4) = e-148
 Identities = 81/113 (71%), Positives = 85/113 (75%)
 Frame = +3

Query: 3   MERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSIEARXXXXXXXXXXXXXXXXXXXX 182
           MERVSGVNIVPHFCRIEDKD++FY  FNIIALGLDSIEAR                    
Sbjct: 106 MERVSGVNIVPHFCRIEDKDIEFYNHFNIIALGLDSIEAR-------------------- 145

Query: 183 XXXXXSYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTP 341
                SYIN+VACSFLEYDSDDKP EETIKPMVDGGTEGFKGHARVI+PGVTP
Sbjct: 146 -----SYINAVACSFLEYDSDDKPLEETIKPMVDGGTEGFKGHARVIIPGVTP 193



 Score = 50.1 bits (118), Expect(4) = e-148
 Identities = 25/48 (52%), Positives = 28/48 (58%)
 Frame = +3

Query: 720 GVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNGLEGL 863
           GVVK                CALE LKIV+ CS+ +SNYLTYNG EGL
Sbjct: 275 GVVKNIIPAIASTNAIISAACALETLKIVSGCSKTLSNYLTYNGAEGL 322


>ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [Ricinus communis]
            gi|223543819|gb|EEF45347.1| ubiquitin-activating enzyme
            E1c, putative [Ricinus communis]
          Length = 449

 Score =  214 bits (546), Expect(4) = e-143
 Identities = 97/134 (72%), Positives = 121/134 (90%)
 Frame = +1

Query: 925  YNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQ 1104
            YNG+EGLH K+TEFV+DKDCL CGPG+L+EL+TS+ LQKF++L+ EHPKL LS+ SVT++
Sbjct: 316  YNGVEGLHIKVTEFVKDKDCLACGPGVLIELDTSVALQKFIDLLEEHPKLFLSRASVTHR 375

Query: 1105 GKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDKKTSCLRKLRIVFK 1284
            GK LYMQAPPVLEEMTRSNL LPLF+LM K+ KD++HVTGMT ++ KKTSC+RKLR+VFK
Sbjct: 376  GKNLYMQAPPVLEEMTRSNLSLPLFELMGKVPKDIVHVTGMTGQNGKKTSCVRKLRVVFK 435

Query: 1285 GVDGITDVDMASGA 1326
            G++GITD+DMA GA
Sbjct: 436  GMEGITDMDMAGGA 449



 Score =  166 bits (421), Expect(4) = e-143
 Identities = 73/81 (90%), Positives = 78/81 (96%)
 Frame = +1

Query: 415 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDADDPEHMQWIY 594
           CFECTIWLFPPQVKFPLCTLAETPRT AHCIEYAHLIKW+EVHSGKAFD DDPEHM+W+Y
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTPAHCIEYAHLIKWNEVHSGKAFDPDDPEHMKWVY 253

Query: 595 SEALKRAELFGIPGVTYSLTQ 657
           +EA+KRAELFGI GVTYSLTQ
Sbjct: 254 TEAVKRAELFGIQGVTYSLTQ 274



 Score =  149 bits (376), Expect(4) = e-143
 Identities = 74/113 (65%), Positives = 84/113 (74%)
 Frame = +3

Query: 3   MERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSIEARXXXXXXXXXXXXXXXXXXXX 182
           MERVSGVNIVPHFCRIEDK+LDFY +F+II LGLDSIEAR                    
Sbjct: 106 MERVSGVNIVPHFCRIEDKELDFYNDFSIIVLGLDSIEAR-------------------- 145

Query: 183 XXXXXSYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTP 341
                SYIN+VACSFLEYDS+D P ++T+KPMVDGGTEGFKGHARVI+PG+TP
Sbjct: 146 -----SYINNVACSFLEYDSEDNPRDDTMKPMVDGGTEGFKGHARVIMPGITP 193



 Score = 49.3 bits (116), Expect(4) = e-143
 Identities = 24/48 (50%), Positives = 28/48 (58%)
 Frame = +3

Query: 720 GVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNGLEGL 863
           GVVK                CALE LKI + CS+ +SNYLTYNG+EGL
Sbjct: 275 GVVKNIIPAIASTNAIISAACALETLKIASGCSKTLSNYLTYNGVEGL 322


>ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit [Medicago truncatula]
            gi|355511444|gb|AES92586.1| NEDD8-activating enzyme E1
            catalytic subunit [Medicago truncatula]
          Length = 454

 Score =  214 bits (546), Expect(4) = e-142
 Identities = 101/134 (75%), Positives = 119/134 (88%)
 Frame = +1

Query: 925  YNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQ 1104
            YNG EGLHTK+TEF RDKDCLVCGPGI +EL+ SITLQKFM+L+ EHPKL LSK SVT++
Sbjct: 321  YNGSEGLHTKVTEFERDKDCLVCGPGIRIELDPSITLQKFMDLLEEHPKLRLSKASVTHR 380

Query: 1105 GKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDKKTSCLRKLRIVFK 1284
            GK LYMQAPPVLEEMTRSNL L LF+LM K+ KD++HV GMT+K+D+KTSCLRKLR+VFK
Sbjct: 381  GKNLYMQAPPVLEEMTRSNLTLSLFNLMGKLPKDIVHVNGMTSKNDQKTSCLRKLRVVFK 440

Query: 1285 GVDGITDVDMASGA 1326
            G+DG+TD+D A GA
Sbjct: 441  GIDGVTDMDTAGGA 454



 Score =  160 bits (405), Expect(4) = e-142
 Identities = 70/81 (86%), Positives = 75/81 (92%)
 Frame = +1

Query: 415 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDADDPEHMQWIY 594
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH G  FD D+PE+M+W+Y
Sbjct: 199 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHRGVPFDPDNPENMKWVY 258

Query: 595 SEALKRAELFGIPGVTYSLTQ 657
            EA+KRAELFGIPGVTYS TQ
Sbjct: 259 DEAVKRAELFGIPGVTYSFTQ 279



 Score =  153 bits (386), Expect(4) = e-142
 Identities = 76/113 (67%), Positives = 85/113 (75%)
 Frame = +3

Query: 3   MERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSIEARXXXXXXXXXXXXXXXXXXXX 182
           MER+SGVNIVPHFCRIEDK+++FY +F+IIALGLDSIEAR                    
Sbjct: 111 MERISGVNIVPHFCRIEDKEIEFYNDFSIIALGLDSIEAR-------------------- 150

Query: 183 XXXXXSYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTP 341
                SYIN+VACSFLEYDSDD P EETIKPMVDGGTEGFKGHARVI+PG+TP
Sbjct: 151 -----SYINTVACSFLEYDSDDNPREETIKPMVDGGTEGFKGHARVIMPGITP 198



 Score = 49.3 bits (116), Expect(4) = e-142
 Identities = 25/48 (52%), Positives = 27/48 (56%)
 Frame = +3

Query: 720 GVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNGLEGL 863
           GVVK                CALE LKI T CS+ +SNYLTYNG EGL
Sbjct: 280 GVVKNIIPAIASTNAIISAACALETLKIATECSKTLSNYLTYNGSEGL 327


>ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|222851659|gb|EEE89206.1|
            predicted protein [Populus trichocarpa]
          Length = 449

 Score =  213 bits (542), Expect(4) = e-142
 Identities = 99/134 (73%), Positives = 118/134 (88%)
 Frame = +1

Query: 925  YNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEHPKLLLSKPSVTYQ 1104
            YNG+EGLH K+TEFV+DKDCLVCGPG+LLEL+TS+TLQKF++++ EHPKLLLSK SV ++
Sbjct: 316  YNGVEGLHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLEEHPKLLLSKASVRHR 375

Query: 1105 GKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDKKTSCLRKLRIVFK 1284
               LYMQAPPVLEEMTRSNL LPLF+LM K  KD++HVTG T+KDDKKTSCLRKL +VFK
Sbjct: 376  ATNLYMQAPPVLEEMTRSNLNLPLFELMGKFPKDIVHVTGTTSKDDKKTSCLRKLCLVFK 435

Query: 1285 GVDGITDVDMASGA 1326
            G D +TD+DMA GA
Sbjct: 436  GADAVTDLDMAVGA 449



 Score =  169 bits (427), Expect(4) = e-142
 Identities = 74/81 (91%), Positives = 78/81 (96%)
 Frame = +1

Query: 415 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFDADDPEHMQWIY 594
           CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK FD DDPEHM+W+Y
Sbjct: 194 CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKTFDPDDPEHMKWVY 253

Query: 595 SEALKRAELFGIPGVTYSLTQ 657
           +EA+KRAELFGI GVTYSLTQ
Sbjct: 254 TEAVKRAELFGIQGVTYSLTQ 274



 Score =  148 bits (373), Expect(4) = e-142
 Identities = 74/113 (65%), Positives = 82/113 (72%)
 Frame = +3

Query: 3   MERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSIEARXXXXXXXXXXXXXXXXXXXX 182
           MERVSGVNIVPHFCRIEDK++DFY++F II LGLDS+EAR                    
Sbjct: 106 MERVSGVNIVPHFCRIEDKEIDFYKDFIIIVLGLDSVEAR-------------------- 145

Query: 183 XXXXXSYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTP 341
                SYIN+VAC FLEYDSDD P EET+KPMVDGGTEGFKGHARVI+PG TP
Sbjct: 146 -----SYINAVACGFLEYDSDDNPIEETVKPMVDGGTEGFKGHARVIIPGSTP 193



 Score = 47.0 bits (110), Expect(4) = e-142
 Identities = 22/48 (45%), Positives = 27/48 (56%)
 Frame = +3

Query: 720 GVVKXXXXXXXXXXXXXXXXCALEALKIVTACSRIMSNYLTYNGLEGL 863
           GVVK                C LE LK+ + CS+ +SNYLTYNG+EGL
Sbjct: 275 GVVKNIIPAIASTNAIISAACTLETLKLASGCSKTLSNYLTYNGVEGL 322