BLASTX nr result
ID: Coptis25_contig00004151
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00004151
(1840 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica] 532 e-148
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum] 523 e-146
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 500 e-139
ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]... 498 e-138
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta] 489 e-136
>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
Length = 524
Score = 532 bits (1371), Expect = e-148
Identities = 268/530 (50%), Positives = 377/530 (71%), Gaps = 3/530 (0%)
Frame = +3
Query: 12 MDFTMLIQWLLTSLATLVAFVLIWYSATSSSTRKINK--TAPVAAGALPIVGHLHMLMGR 185
MD ML S+A+++AFV + Y+ SS T K K TAP+A GA P++GHLH+
Sbjct: 1 MDSLMLAYLFPISVASIIAFVFL-YNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSG 59
Query: 186 ELPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAVFKRPRSKALKNLT 365
ELPH++L+ MADKYG AF MK+G LVVS ++ KECF ND RP +KA + +T
Sbjct: 60 ELPHKMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMT 119
Query: 366 YDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNN 545
YD S F PYG W E+RK++ LLSN RLQ + VRASEV+ K LYD + S +
Sbjct: 120 YDNESVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYDQCKNPSGS 179
Query: 546 NKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVVVDATLLTAK 725
AP+L++M KWFEE++ NVV R++ G+++ GSK + E+ E++HYKKV+ + L +
Sbjct: 180 ---APILIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEE-EAIHYKKVMDELLRLASL 235
Query: 726 LVVSDFFPSLGLVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVSGSEDDEQDFIDLT 905
+ SDF P LG VD QG+ S++K +K++DAIL W+EEHR KK S +E +QDF+D+
Sbjct: 236 SMFSDFAPLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKKNSVAES-QQDFMDVM 294
Query: 906 LSMIDQTQHQGADVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNNRPVLRKAREEIDR 1085
LS++++++ G D D IK+ C+ MI GG+D+T+V+LTW +S+LMNNR L+KAREE+D
Sbjct: 295 LSIVEESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKAREELDA 354
Query: 1086 QVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPLLERVTVEDCEIGGYHLKTGSRVIVN 1265
VG DR+V D D+ L ++ AI+KE+MR+ +G LLER T EDCEI G+H+K G+R++VN
Sbjct: 355 LVGKDRQVEDSDLKNLVYMNAIVKETMRMYPLGTLLERETKEDCEIDGFHVKGGTRLLVN 414
Query: 1266 IWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGITFALELIQL 1445
+WK+Q DPN+W DP EFRPERFLT NA++++ GQH+ELLPFGAG R+CPG+ FAL+ + L
Sbjct: 415 VWKLQRDPNVWVDPTEFRPERFLTENADIDVGGQHFELLPFGAGRRVCPGVXFALQFMHL 474
Query: 1446 TLARLIHGFELGTPMDADVDMTET-SSVTNYRATPLEILLTPRLDPKLYD 1592
LARLIHG++L T + +VD+TE+ N++A+PL+++LTPRL KLY+
Sbjct: 475 VLARLIHGYDLNTLNEENVDLTESPEGHVNHKASPLDLILTPRLHYKLYE 524
>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
Length = 541
Score = 523 bits (1347), Expect = e-146
Identities = 262/543 (48%), Positives = 367/543 (67%), Gaps = 16/543 (2%)
Frame = +3
Query: 12 MDFTMLIQWLLT------SLATLVAFVLIWY---------SATSSSTRKINKTAPVAAGA 146
MDF+ L+ LL S+A L+A VL++ TS +KI P GA
Sbjct: 1 MDFSSLLLLLLNTWISAYSMAALLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAVTGA 60
Query: 147 LPIVGHLHMLMGRELPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAV 326
P+ GHLH+ E PH++LS +A+KYGP+F MK+G LVVS ++ KECF ND
Sbjct: 61 WPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTNDTLF 120
Query: 327 FKRPRSKALKNLTYDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFI 506
RP + A +TY S F PY P W E+RK++ LLSN RL+ + +R SEV
Sbjct: 121 SNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVSVCF 180
Query: 507 KELYDLWSSNSNNNKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHY 686
KELYDL +N N+ GAP+ +++ +WF+E++ NV+ R++ GK++ GSK ED E++HY
Sbjct: 181 KELYDL--TNKKNDNGAPVPIDLKRWFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHY 238
Query: 687 KKVVVDATLLTAKLVVSDFFPSLGLVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVS 866
KK++ + + L++ ++SD P LG +DY +GD ++K KEL++IL W+EEH+ KK
Sbjct: 239 KKIMDELSRLSSLTMLSDMVPLLGWLDYFKGDLRAMKRNGKELNSILQKWLEEHKSKK-- 296
Query: 867 GSEDDEQDFIDLTLSMIDQTQHQGADVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNN 1046
S D QDF+D+ LS+ TQ G D DTFIK+ C+ MI GG++ST VALTW LS+LMNN
Sbjct: 297 -SSDARQDFMDVMLSISKDTQLYGHDQDTFIKATCLAMIMGGTNSTEVALTWILSLLMNN 355
Query: 1047 RPVLRKAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPLLERVTVEDCEIG 1226
R L KAREEID VG DR+V D DV L ++ AIIKE+MR+ +G LLER T EDCE+
Sbjct: 356 RCALHKAREEIDLLVGKDRQVEDSDVKNLTYMNAIIKETMRLYPLGFLLERDTKEDCEVS 415
Query: 1227 GYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRI 1406
G+++K G+R+++N+WK+Q DPN+W DP EF+PERFLT NA++++ GQH+ELLPFGAG R+
Sbjct: 416 GFNIKGGTRLLINVWKLQRDPNVWTDPMEFKPERFLTENADIDVGGQHFELLPFGAGRRV 475
Query: 1407 CPGITFALELIQLTLARLIHGFELGTPMDADVDMTETS-SVTNYRATPLEILLTPRLDPK 1583
CPG++FAL+ + L LARLIHG+++ T DVD++ +S N ++TPLE++LTPRL P+
Sbjct: 476 CPGVSFALQFMHLVLARLIHGYDMETLNGEDVDLSVSSGGHVNIKSTPLELILTPRLHPE 535
Query: 1584 LYD 1592
LYD
Sbjct: 536 LYD 538
>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|302142392|emb|CBI19595.3| unnamed protein product
[Vitis vinifera]
Length = 527
Score = 500 bits (1287), Expect = e-139
Identities = 257/499 (51%), Positives = 360/499 (72%), Gaps = 6/499 (1%)
Frame = +3
Query: 117 NKTAPVAAGALPIVGHLHMLMGRE-LPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLA 293
++ AP GA PI+GHLH+L G + L ++ L MADKYGPAF ++ GS+ A VVSS ++A
Sbjct: 35 SRDAPEPGGAWPIIGHLHLLGGGDQLLYRTLGAMADKYGPAFNIRLGSRRAFVVSSWEVA 94
Query: 294 KECFVHNDRAVFKRPRSKALKNLTYDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQR 473
KECF ND+A+ RP + A K++ Y+ A FGFAPY P W EMRK+A LLSN+RL++ +
Sbjct: 95 KECFTINDKALATRPTTVAAKHMGYNYAVFGFAPYSPFWREMRKIATLELLSNRRLEILK 154
Query: 474 HVRASEVDAFIKELYDLWSSNSNNNKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKA 653
HVR SEVD I+ELY LW NS+ PLLVE+N+W E++ LNV+ RMV+GKR+ G+ A
Sbjct: 155 HVRTSEVDMGIRELYGLWVKNSSR----PLLVELNRWLEDMTLNVIVRMVAGKRYFGAAA 210
Query: 654 RRSEDSESMHYKKVVVDATLLTAKLVVSDFFPSLGLVDYLQGDESSIKGTSKELDAILAT 833
S+ SE+ +K + L VVSD P L +D LQG E ++K T+KELD+IL
Sbjct: 211 A-SDSSEARRCQKAISQFFRLIGIFVVSDALPFLWWLD-LQGHERAMKTTAKELDSILEG 268
Query: 834 WVEEHRHKKVSG--SEDDEQDFIDLTLSMIDQTQHQGA--DVDTFIKSMCVGMIFGGSDS 1001
W+EEHR ++VS + EQDFID+ LS+ ++ + G D +T IKS C+ +I GGSD+
Sbjct: 269 WLEEHRQRRVSSLIKAEGEQDFIDVMLSLQEEGRLSGFQYDSETSIKSTCLALILGGSDT 328
Query: 1002 TSVALTWALSILMNNRPVLRKAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLV 1181
T+ LTWA+S+L+NNR L+KA+EE+D VG +R+V + DV L +L+AIIKE++R+
Sbjct: 329 TAGTLTWAISLLLNNRHALKKAQEELDLCVGMERQVEESDVKNLVYLQAIIKETLRLYPA 388
Query: 1182 GPLL-ERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVEL 1358
GPLL R ++DC + GY++ G+R+IVNIWK+Q DP++W +P F+PERFL +A+V++
Sbjct: 389 GPLLGPREALDDCTVAGYNVPAGTRLIVNIWKLQRDPSVWTNPCAFQPERFLNAHADVDV 448
Query: 1359 RGQHYELLPFGAGSRICPGITFALELIQLTLARLIHGFELGTPMDADVDMTETSSVTNYR 1538
+GQ +EL+PFG+G R CPG++FAL+++ LTLARL+H FEL TP+D VDMTE+S +T +
Sbjct: 449 KGQQFELMPFGSGRRSCPGVSFALQVLHLTLARLLHAFELSTPVDQPVDMTESSGLTIPK 508
Query: 1539 ATPLEILLTPRLDPKLYDY 1595
ATPLE+LLTPRL+ KLY +
Sbjct: 509 ATPLEVLLTPRLNSKLYAF 527
>ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]
gi|223550999|gb|EEF52485.1| cytochrome P450, putative
[Ricinus communis]
Length = 523
Score = 498 bits (1283), Expect = e-138
Identities = 249/524 (47%), Positives = 366/524 (69%), Gaps = 8/524 (1%)
Frame = +3
Query: 42 LTSLATLVAFVLI---WYSATSSSTRKINKTAPVAAGALPIVGHLHMLMGRE-LPHQLLS 209
LT++A +++F+ + W S ++ AP AG+ PI+GHLH+L G + L H+ L
Sbjct: 7 LTAIAVIISFIFVCSTWLRGKKKSVGSKSREAPEPAGSWPIIGHLHLLGGADKLLHRTLG 66
Query: 210 NMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAVFKRPRSKALKNLTYDQASFGF 389
++ADKYGPAF ++ GS ALVV+S +LAKECF ND+ + R + A K++ YD A FGF
Sbjct: 67 SLADKYGPAFNIRIGSHRALVVASKELAKECFTINDKTLASRSTTAATKHMCYDHAVFGF 126
Query: 390 APYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNNNKGAPLLV 569
APY W EMRK+ LLSN+RL+M +HV+ASEVD I++LY+LW+ N + P++V
Sbjct: 127 APYSSHWREMRKIVMLELLSNRRLEMVKHVQASEVDLGIRKLYNLWAQN----RCLPVIV 182
Query: 570 EMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVVVDATLLTAKLVVSDFFP 749
E+ ++FE+L L+V+ R+V+GKR+ GS S+D E+ Y+K + L VVSD P
Sbjct: 183 ELKQFFEDLTLDVIVRVVAGKRYTGS----SDDDEARQYQKAISQFFHLMGIFVVSDALP 238
Query: 750 SLGLVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVSGS--EDDEQDFIDLTLSMIDQ 923
L +D L+G E ++K T+K+LDA+LA W++EHR ++VSG + +QDFID+ LS+ ++
Sbjct: 239 FLRWLD-LEGHEKAMKKTAKDLDAVLAGWLDEHRRRRVSGEVKSEGDQDFIDVMLSLEEK 297
Query: 924 TQHQGA--DVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNNRPVLRKAREEIDRQVGT 1097
G D DT IKS C+ +I G SD+T+ L WA+S+L+NN+ L+ A+EE+++ +GT
Sbjct: 298 GHLSGFQYDADTSIKSTCLALIAGASDTTTTTLVWAISLLLNNQLALKNAKEELEKHIGT 357
Query: 1098 DRKVNDLDVLKLDHLKAIIKESMRICLVGPLLERVTVEDCEIGGYHLKTGSRVIVNIWKM 1277
+R+V++ D+ L +L+A+IKE++R+ V PL+ R VEDC +GGYH+ G+R++VN+WK+
Sbjct: 358 ERQVDESDLKNLVYLQAVIKETLRLYPVAPLIPREFVEDCRVGGYHVPAGTRLLVNVWKI 417
Query: 1278 QHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGITFALELIQLTLAR 1457
Q DP LW F+PERFLT++A++++RG H+ELLPFG+G R CPG +FAL + LTLAR
Sbjct: 418 QRDPMLWTKASAFQPERFLTSHADIDVRGHHFELLPFGSGRRSCPGASFALHALHLTLAR 477
Query: 1458 LIHGFELGTPMDADVDMTETSSVTNYRATPLEILLTPRLDPKLY 1589
+H F++ TPMD VDMTE S T +ATPLE+LL+PRL KLY
Sbjct: 478 FLHAFDVATPMDQPVDMTERSGTTLPKATPLEVLLSPRLPAKLY 521
>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 511
Score = 489 bits (1260), Expect = e-136
Identities = 243/508 (47%), Positives = 350/508 (68%), Gaps = 1/508 (0%)
Frame = +3
Query: 69 FVLIWYSATSSSTRKINKTAPVAAGALPIVGHLHMLMGRELPHQLLSNMADKYGPAFMMK 248
F+++W + +SS T K AP A GA PI+GHLH+L G EL H+ L MADKYGP F ++
Sbjct: 7 FLILWRTRSSSKTNTC-KEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPIFKIR 65
Query: 249 YGSQPALVVSSLKLAKECFVHNDRAVFKRPRSKALKNLTYDQASFGFAPYGPLWVEMRKV 428
G ALVVS+ +AKECF ND+A RP S A K L YD FG APYG WVE+RK+
Sbjct: 66 IGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVELRKI 125
Query: 429 AKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNNNKGAPLLVEMNKWFEELALNV 608
+ LLSN+RL++ +HVR SE+DA I++LY +W N + KG P+LV+M +WF +L LNV
Sbjct: 126 TMSELLSNRRLELLKHVRDSEIDASIQDLYKVWK-NHDKAKG-PVLVDMKQWFGDLTLNV 183
Query: 609 VTRMVSGKRHIGSKARRSEDSESMHYKKVVVDATLLTAKLVVSDFFPSLGLVDYLQGDES 788
+ RM++GKR+ GS + +++E+ +K + D L ++ D P L D LQG +
Sbjct: 184 ILRMIAGKRYSGSMSS-CDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWFD-LQGYKK 241
Query: 789 SIKGTSKELDAILATWVEEHRHKKVSGSEDDEQDFIDLTLSMIDQTQHQGADVDTFIKSM 968
+K T+KELD++ W+EEH + +G + EQDF+D+ +S+++ T+ D DT IKS
Sbjct: 242 EMKNTAKELDSVFQRWLEEHNRMRQTGELNREQDFMDVLMSILEDTRISEYDNDTIIKST 301
Query: 969 CVGMIFGGSDSTSVALTWALSILMNNRPVLRKAREEIDRQVGTDRKVNDLDVLKLDHLKA 1148
C+ ++ GG D+T V LTW LS+L+NN+ L+KA++E+D VG DR+V + D+ L +L+A
Sbjct: 302 CLSIVTGGGDTTMVTLTWILSLLLNNKHALKKAQDELDSHVGKDRQVEESDIKNLVYLQA 361
Query: 1149 IIKESMRICLVGPLL-ERVTVEDCEIGGYHLKTGSRVIVNIWKMQHDPNLWPDPFEFRPE 1325
I KE++R+ GPL RV DC + GYH+ G+R+IVN +K+Q DP +W +P EFRPE
Sbjct: 362 ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE 421
Query: 1326 RFLTTNANVELRGQHYELLPFGAGSRICPGITFALELIQLTLARLIHGFELGTPMDADVD 1505
RFLT++ N++++G HYEL+PFGAG R CPG++F L+++ L LAR +H F+ T MD VD
Sbjct: 422 RFLTSHVNMDVKGLHYELIPFGAGRRSCPGMSFTLQVVPLVLARFLHEFDSKTEMDMPVD 481
Query: 1506 MTETSSVTNYRATPLEILLTPRLDPKLY 1589
MTET+ +TN +ATPLE+++TPRL P++Y
Sbjct: 482 MTETAGLTNAKATPLEVVITPRLHPEIY 509