BLASTX nr result
ID: Coptis25_contig00001273
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00001273
(1091 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 164 3e-63
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 145 4e-54
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 142 3e-53
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 141 2e-52
gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimb... 127 1e-46
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT gi|226897702|gb|ACO90222.1|
tetrahydroprotoberberine N-methyltransferase
[Eschscholzia californica]
Length = 350
Score = 164 bits (414), Expect(2) = 3e-63
Identities = 75/110 (68%), Positives = 91/110 (82%)
Frame = +2
Query: 332 EDWYSNYIFPKGSVTIPGASTLLYFQDDVAVADQWVENGKHMARTVEEWLRKLDKNIDAV 511
+DWYS +IFPKG VTI +STLLYFQDDV+ D WV NG HMAR+VE W +KLD+ I+A
Sbjct: 241 DDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAA 300
Query: 512 RKILEPCLGSKDAVDKVIIHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 661
R+ILEP LGSK+AV++VI H RTFCIG YEQFS N+G+EWMI+ ILF+KK
Sbjct: 301 REILEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFKKK 350
Score = 105 bits (262), Expect(2) = 3e-63
Identities = 44/72 (61%), Positives = 62/72 (86%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCNNLELSNVDVILADVTKYDTNETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+AQ + IE++ LEL+NVDVI ADVTK+DT++T+DRI+V+E +EHMK
Sbjct: 143 YKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKTYDRILVVETIEHMK 202
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 203 NIQLFMKKLSTW 214
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
tetrahydroprotoberberine N-methyltransferase [Papaver
bracteatum]
Length = 358
Score = 145 bits (366), Expect(2) = 4e-54
Identities = 66/111 (59%), Positives = 86/111 (77%), Gaps = 1/111 (0%)
Frame = +2
Query: 332 EDWYSNYIFPKGSVTIPGASTLLYFQDDVAVADQWVENGKHMARTVEEWLRKLDKNIDAV 511
+DWYS +IFP G TI A++LLYFQDDV+V D WV NG HMAR+V+ W + LDKN++A
Sbjct: 248 DDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAA 307
Query: 512 RKILEPCL-GSKDAVDKVIIHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 661
++IL P L GS +AV+ V+ H RTFC+G YEQFS NDG EWM++ +LF+KK
Sbjct: 308 KEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFKKK 358
Score = 93.6 bits (231), Expect(2) = 4e-54
Identities = 39/72 (54%), Positives = 61/72 (84%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCNNLELSNVDVILADVTKYDTNETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+AQ + + ++ L L+NVD ILADVT+Y++++T+DR+++IEA+EHMK
Sbjct: 150 YKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMK 209
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 210 NLQLFMKKLSTW 221
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 142 bits (357), Expect(2) = 3e-53
Identities = 64/111 (57%), Positives = 85/111 (76%), Gaps = 1/111 (0%)
Frame = +2
Query: 332 EDWYSNYIFPKGSVTIPGASTLLYFQDDVAVADQWVENGKHMARTVEEWLRKLDKNIDAV 511
+DWYS +IFP G TI A++LLYFQDDV+V D WV NG HMAR+V+ W + LDKN++A
Sbjct: 248 DDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAA 307
Query: 512 RKILEPCL-GSKDAVDKVIIHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 661
++IL P L GS + V+ V+ H RTFC+G YEQFS N+G EWM++ +LF+KK
Sbjct: 308 KEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLFKKK 358
Score = 94.4 bits (233), Expect(2) = 3e-53
Identities = 39/72 (54%), Positives = 61/72 (84%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCNNLELSNVDVILADVTKYDTNETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+AQ + + ++ L L+NVD ILADVT+Y++++T+DR+++IEA+EHMK
Sbjct: 150 YKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMK 209
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 210 NLQLFMKKLSTW 221
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2
gi|226897730|gb|ACO90236.1| putative N-methyltransferase
[Papaver bracteatum]
Length = 358
Score = 141 bits (355), Expect(2) = 2e-52
Identities = 66/111 (59%), Positives = 82/111 (73%), Gaps = 1/111 (0%)
Frame = +2
Query: 332 EDWYSNYIFPKGSVTIPGASTLLYFQDDVAVADQWVENGKHMARTVEEWLRKLDKNIDAV 511
+DWYS YIFP G T A +LLYFQDDV+V D WV NG H ARTV+ W +KLDKN++AV
Sbjct: 248 DDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAV 307
Query: 512 RKILEPCL-GSKDAVDKVIIHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 661
++IL P L G+ +AV+ VI H RT C+G Y QFS NDG EWM + +LF+KK
Sbjct: 308 KEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLFKKK 358
Score = 92.0 bits (227), Expect(2) = 2e-52
Identities = 41/72 (56%), Positives = 58/72 (80%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCNNLELSNVDVILADVTKYDTNETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+ Q + I ++ L L NVDVILADVT+Y++++T+DRI+VI VEHMK
Sbjct: 150 YKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQYESDKTYDRILVIGVVEHMK 209
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 210 NMQLFIKKLSTW 221
>gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimbriata]
Length = 357
Score = 127 bits (318), Expect(2) = 1e-46
Identities = 56/110 (50%), Positives = 76/110 (69%)
Frame = +2
Query: 332 EDWYSNYIFPKGSVTIPGASTLLYFQDDVAVADQWVENGKHMARTVEEWLRKLDKNIDAV 511
+DW++ Y FP GS +P AS LLYFQDDV++ D W+ +GKH RT EEW+++LD N++
Sbjct: 248 DDWFAEYFFPPGSFAMPSASFLLYFQDDVSIVDHWILSGKHFHRTAEEWVKQLDTNLEKG 307
Query: 512 RKILEPCLGSKDAVDKVIIHSRTFCIGAYEQFSCNDGQEWMISHILFRKK 661
++ILE GSK+A K H R CI + E F N G+EWM SH+LF+KK
Sbjct: 308 KEILESKYGSKEAALKAFNHWRGLCIFSSEIFGYNGGEEWMTSHLLFKKK 357
Score = 87.0 bits (214), Expect(2) = 1e-46
Identities = 41/72 (56%), Positives = 52/72 (72%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCNNLELSNVDVILADVTKYDTNETFDRIIVIEAVEHMK 182
+K HVT +TNS QK IE++C LELSNV+V+L D+T+ TFDRIIVI +EHMK
Sbjct: 149 YKKSHVTAVTNSATQKKYIEDQCQILELSNVEVLLEDITQLTMEATFDRIIVIGLLEHMK 208
Query: 183 NVGLFLRKISKW 218
N GL L+ IS+W
Sbjct: 209 NYGLLLQNISQW 220