BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00001236
         (1499 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bon...   520   e-145
gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]              513   e-143
pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Red...   503   e-140
dbj|BAA89423.1| allyl alcohol dehydrogenase [Nicotiana tabacum]       503   e-140
gb|ACN65116.1| 2-alkenal reductase [Artemisia annua]                  499   e-139

>ref|XP_002279359.1| PREDICTED: NADP-dependent alkenal double bond reductase P2 [Vitis
            vinifera] gi|147792339|emb|CAN61471.1| hypothetical
            protein VITISV_043825 [Vitis vinifera]
          Length = 345

 Score =  520 bits (1338), Expect = e-145
 Identities = 249/337 (73%), Positives = 289/337 (85%)
 Frame = +1

Query: 52   NKQIVMLDYSTGLPKESDMRVHTSTTPLKVPEGSNVLLVKNLYLSCDPYMRTRMKKIADS 231
            NKQ++  DY +G PKESDM V TST  LKVPEGS  +LVKNLYLSCDPYMR+RM+ I  S
Sbjct: 9    NKQVIFRDYVSGFPKESDMYVTTSTISLKVPEGSEAVLVKNLYLSCDPYMRSRMRNIQGS 68

Query: 232  HYPGFVPGEPLTGLGVVKILDSGHPDFKEGDLAWGMTGWEEYTLIHDPQSLVKITYTDLP 411
            +   F PG P+TG GV K+LDS HPDF+ GDL WG+TGWEEY+LI   + L KI +TD+P
Sbjct: 69   YVEPFKPGSPITGYGVAKVLDSRHPDFRTGDLVWGITGWEEYSLITTDERLFKIQHTDVP 128

Query: 412  LSYYTGILGMTGLAAYVGFYEVCSPKKGEHVFVSAASGAVGQLVGQFAKLMGCHVVGSAG 591
            LSYYTGILGM G+ AY GFYE+CSPKKGE+VF+SAASGAVGQLVGQFAKL+GC+VVGSAG
Sbjct: 129  LSYYTGILGMAGMTAYSGFYEICSPKKGEYVFISAASGAVGQLVGQFAKLLGCYVVGSAG 188

Query: 592  SNEKVELLKNKFGFDDAFNYKEEPDLAATLKRYFPTGIDIYFENVGGKMLDAVLLNMNVN 771
            + EKV+LLKNKFGFD+AFNYKEE DL A LKRYFP GIDIYFENVGGKMLDAVL+NM ++
Sbjct: 189  TKEKVDLLKNKFGFDEAFNYKEEQDLEACLKRYFPEGIDIYFENVGGKMLDAVLVNMRLH 248

Query: 772  GRISVSGMISQYNLEKPEGVHNLYCIVTKIIRMEGFVVFNHYHLYPKFLELMRQYIKEGK 951
            GRI+V GMISQYNLE+PEGV NL+ IVTK IRMEGF+VF++YHLYPKFL+L+  YI+EGK
Sbjct: 249  GRIAVCGMISQYNLEEPEGVRNLFTIVTKRIRMEGFLVFDYYHLYPKFLDLIMPYIREGK 308

Query: 952  IFYVEDIAEGLESGPAALIGLFTGRNLGKQVVVVAHD 1062
            I YVEDIAEGLES P ALIGL++GRN+GKQVVVVA +
Sbjct: 309  IVYVEDIAEGLESAPTALIGLYSGRNVGKQVVVVARE 345


>gb|ADO51748.1| alcohol dehydrogenase [Camellia sinensis]
          Length = 347

 Score =  513 bits (1320), Expect = e-143
 Identities = 242/338 (71%), Positives = 291/338 (86%), Gaps = 1/338 (0%)
 Frame = +1

Query: 52   NKQIVMLDYSTGLPKESDMRVHTSTTPLKVPEGS-NVLLVKNLYLSCDPYMRTRMKKIAD 228
            NKQI++ +Y +G PK SDM V T T  L++PEGS N +LVKNLYLSCDPYM  RM+K+ D
Sbjct: 10   NKQIILKEYVSGFPKGSDMVVKTGTIHLQLPEGSKNAILVKNLYLSCDPYMCRRMRKLED 69

Query: 229  SHYPGFVPGEPLTGLGVVKILDSGHPDFKEGDLAWGMTGWEEYTLIHDPQSLVKITYTDL 408
            S+   F PG P+TG GV ++LDSGHP+FK+GDL WG TGWEEYT+I +P ++ KI  TD+
Sbjct: 70   SYVESFTPGSPITGFGVARVLDSGHPNFKKGDLIWGRTGWEEYTIITEPANVFKIQNTDV 129

Query: 409  PLSYYTGILGMTGLAAYVGFYEVCSPKKGEHVFVSAASGAVGQLVGQFAKLMGCHVVGSA 588
            PLSYYTGILGM G+ AYVGFYE+CSPK+G++VF+SAASGAVGQLVGQFAKL+GC+VVGSA
Sbjct: 130  PLSYYTGILGMHGMTAYVGFYEICSPKQGDYVFISAASGAVGQLVGQFAKLLGCYVVGSA 189

Query: 589  GSNEKVELLKNKFGFDDAFNYKEEPDLAATLKRYFPTGIDIYFENVGGKMLDAVLLNMNV 768
            G+ EKV+LLKNKFGFD+AFNYKEE DL A LKRYFP GI+IYFENVGGKMLDAVL NM +
Sbjct: 190  GTKEKVDLLKNKFGFDEAFNYKEEKDLEAALKRYFPNGINIYFENVGGKMLDAVLANMRL 249

Query: 769  NGRISVSGMISQYNLEKPEGVHNLYCIVTKIIRMEGFVVFNHYHLYPKFLELMRQYIKEG 948
            + RI+V GMISQYNLE+PEGVHNL+C++TK +RMEGF+VF++YHLYPKFLE++   IK G
Sbjct: 250  HSRIAVCGMISQYNLERPEGVHNLFCLITKRVRMEGFIVFDYYHLYPKFLEMILPCIKGG 309

Query: 949  KIFYVEDIAEGLESGPAALIGLFTGRNLGKQVVVVAHD 1062
            KI YVED+AEGLES PAALIGLF+GRN+GKQVVVVA +
Sbjct: 310  KITYVEDVAEGLESAPAALIGLFSGRNVGKQVVVVARE 347


>pdb|4HFJ|A Chain A, X-ray Crystal Structure Of A Double Bond Reductase From
            Nicotiana Tabacum gi|444302247|pdb|4HFJ|B Chain B, X-ray
            Crystal Structure Of A Double Bond Reductase From
            Nicotiana Tabacum gi|444302248|pdb|4HFM|A Chain A, X-ray
            Crystal Structure Of A Nadp(h)-bound Double Bond
            Reductase From Nicotiana Tabacum gi|444302249|pdb|4HFM|B
            Chain B, X-ray Crystal Structure Of A Nadp(h)-bound
            Double Bond Reductase From Nicotiana Tabacum
            gi|444302250|pdb|4HFN|A Chain A, X-ray Crystal Structure
            Of A Ternary Complex Of Double Bond Reductase From
            Nicotiana Tabacum gi|444302251|pdb|4HFN|B Chain B, X-ray
            Crystal Structure Of A Ternary Complex Of Double Bond
            Reductase From Nicotiana Tabacum
          Length = 351

 Score =  503 bits (1294), Expect = e-140
 Identities = 237/337 (70%), Positives = 286/337 (84%)
 Frame = +1

Query: 52   NKQIVMLDYSTGLPKESDMRVHTSTTPLKVPEGSNVLLVKNLYLSCDPYMRTRMKKIADS 231
            NKQ+++ +Y TG PKESDM +   T  LKVPEGSN ++VKNLYLSCDPYMR+RM+KI  S
Sbjct: 7    NKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRMRKIEGS 66

Query: 232  HYPGFVPGEPLTGLGVVKILDSGHPDFKEGDLAWGMTGWEEYTLIHDPQSLVKITYTDLP 411
            +   F PG P+TG GV K+L+SG P F++GDL WGMTGWEEY++I   Q+L KI   D+P
Sbjct: 67   YVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQTLFKIHDKDVP 126

Query: 412  LSYYTGILGMTGLAAYVGFYEVCSPKKGEHVFVSAASGAVGQLVGQFAKLMGCHVVGSAG 591
            LSYYTGILGM G+ AY GF+EVCSPKKGE VFVSAASGAVGQLVGQFAK++GC+VVGSAG
Sbjct: 127  LSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGSAG 186

Query: 592  SNEKVELLKNKFGFDDAFNYKEEPDLAATLKRYFPTGIDIYFENVGGKMLDAVLLNMNVN 771
            S EKV+LLK+KFGFD+AFNYKEE DL+A LKRYFP GIDIYFENVGGKMLDAVL+NM + 
Sbjct: 187  SKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMKLY 246

Query: 772  GRISVSGMISQYNLEKPEGVHNLYCIVTKIIRMEGFVVFNHYHLYPKFLELMRQYIKEGK 951
            GRI+V GMISQYNLE+ EGVHNL+C++TK IRMEGF+VF++YHLYPK+LE++   IK GK
Sbjct: 247  GRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIPQIKAGK 306

Query: 952  IFYVEDIAEGLESGPAALIGLFTGRNLGKQVVVVAHD 1062
            + YVED+A GLES P AL+GLF+GRN+GKQVV+V+ +
Sbjct: 307  VVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVSRE 343


>dbj|BAA89423.1| allyl alcohol dehydrogenase [Nicotiana tabacum]
          Length = 343

 Score =  503 bits (1294), Expect = e-140
 Identities = 237/337 (70%), Positives = 286/337 (84%)
 Frame = +1

Query: 52   NKQIVMLDYSTGLPKESDMRVHTSTTPLKVPEGSNVLLVKNLYLSCDPYMRTRMKKIADS 231
            NKQ+++ +Y TG PKESDM +   T  LKVPEGSN ++VKNLYLSCDPYMR+RM+KI  S
Sbjct: 7    NKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRMRKIEGS 66

Query: 232  HYPGFVPGEPLTGLGVVKILDSGHPDFKEGDLAWGMTGWEEYTLIHDPQSLVKITYTDLP 411
            +   F PG P+TG GV K+L+SG P F++GDL WGMTGWEEY++I   Q+L KI   D+P
Sbjct: 67   YVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQTLFKIHDKDVP 126

Query: 412  LSYYTGILGMTGLAAYVGFYEVCSPKKGEHVFVSAASGAVGQLVGQFAKLMGCHVVGSAG 591
            LSYYTGILGM G+ AY GF+EVCSPKKGE VFVSAASGAVGQLVGQFAK++GC+VVGSAG
Sbjct: 127  LSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGSAG 186

Query: 592  SNEKVELLKNKFGFDDAFNYKEEPDLAATLKRYFPTGIDIYFENVGGKMLDAVLLNMNVN 771
            S EKV+LLK+KFGFD+AFNYKEE DL+A LKRYFP GIDIYFENVGGKMLDAVL+NM + 
Sbjct: 187  SKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMKLY 246

Query: 772  GRISVSGMISQYNLEKPEGVHNLYCIVTKIIRMEGFVVFNHYHLYPKFLELMRQYIKEGK 951
            GRI+V GMISQYNLE+ EGVHNL+C++TK IRMEGF+VF++YHLYPK+LE++   IK GK
Sbjct: 247  GRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIPQIKAGK 306

Query: 952  IFYVEDIAEGLESGPAALIGLFTGRNLGKQVVVVAHD 1062
            + YVED+A GLES P AL+GLF+GRN+GKQVV+V+ +
Sbjct: 307  VVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVSRE 343


>gb|ACN65116.1| 2-alkenal reductase [Artemisia annua]
          Length = 347

 Score =  499 bits (1286), Expect = e-139
 Identities = 239/338 (70%), Positives = 285/338 (84%), Gaps = 1/338 (0%)
 Frame = +1

Query: 52   NKQIVMLDYSTGLPKESDMRVHTSTT-PLKVPEGSNVLLVKNLYLSCDPYMRTRMKKIAD 228
            NK++++ DY  G PKESDM + TS T  LK+P GSN LLVKNLYLSCDPYMR+RM K   
Sbjct: 10   NKKVILKDYVVGFPKESDMILKTSETMTLKLPAGSNGLLVKNLYLSCDPYMRSRMTKTEG 69

Query: 229  SHYPGFVPGEPLTGLGVVKILDSGHPDFKEGDLAWGMTGWEEYTLIHDPQSLVKITYTDL 408
            S+   F PG PLTG GV K+L+SGH +FK+GDL WG TGWEEY++I+ P+ L KI +TD+
Sbjct: 70   SYVESFTPGSPLTGYGVAKVLESGHANFKKGDLIWGFTGWEEYSIINAPEGLFKIEHTDV 129

Query: 409  PLSYYTGILGMTGLAAYVGFYEVCSPKKGEHVFVSAASGAVGQLVGQFAKLMGCHVVGSA 588
            PLSYYTGILGM G+ AYVGFYE+C+PKKGE+VFVSAASGAVGQLVGQFAKL GC+VVGSA
Sbjct: 130  PLSYYTGILGMPGMTAYVGFYEICTPKKGEYVFVSAASGAVGQLVGQFAKLSGCYVVGSA 189

Query: 589  GSNEKVELLKNKFGFDDAFNYKEEPDLAATLKRYFPTGIDIYFENVGGKMLDAVLLNMNV 768
            G+ EKV+LLKNKFGFD+AFNYKEE DL A LKRYFP GIDIYFENVGG+MLDAVLLNM +
Sbjct: 190  GTKEKVDLLKNKFGFDEAFNYKEEQDLDAALKRYFPEGIDIYFENVGGRMLDAVLLNMRL 249

Query: 769  NGRISVSGMISQYNLEKPEGVHNLYCIVTKIIRMEGFVVFNHYHLYPKFLELMRQYIKEG 948
            +GRISV GMISQYNLE+ EGV NL+ +VTK + M+GF+VF+HYH YPK+LE++   IK G
Sbjct: 250  DGRISVCGMISQYNLEQSEGVRNLFTLVTKRVTMKGFIVFDHYHKYPKYLEMIIPLIKNG 309

Query: 949  KIFYVEDIAEGLESGPAALIGLFTGRNLGKQVVVVAHD 1062
             I Y+EDI EGLE+ PAALIGL++G+N+GKQVVVVAH+
Sbjct: 310  TINYIEDIVEGLENAPAALIGLYSGKNVGKQVVVVAHE 347