BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00000464
         (4015 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002312505.1| predicted protein [Populus trichocarpa] gi|2...   499   e-138
ref|XP_002314739.1| predicted protein [Populus trichocarpa] gi|2...   490   e-135
ref|XP_003520929.1| PREDICTED: NADH-cytochrome b5 reductase 1-li...   390   e-123
gb|AAV69019.1| NADH:cytochrome b5 reductase [Vernicia fordii] gi...   389   e-122
gb|ACU19291.1| unknown [Glycine max]                                  388   e-122

>ref|XP_002312505.1| predicted protein [Populus trichocarpa] gi|222852325|gb|EEE89872.1|
            predicted protein [Populus trichocarpa]
          Length = 721

 Score =  499 bits (1285), Expect = e-138
 Identities = 321/750 (42%), Positives = 414/750 (55%), Gaps = 15/750 (2%)
 Frame = -3

Query: 2429 KRNTDCVYFLASPLTCKKGVECEYRHSEEARMNPRDCWYWLNGNCLNPKCSFRHPPLDGL 2250
            KRNTDCVYFLASPLTCKKG ECEYRHSE AR+NPRDC+YWLNGNCLNPKC FRHPPLDGL
Sbjct: 11   KRNTDCVYFLASPLTCKKGSECEYRHSEYARVNPRDCYYWLNGNCLNPKCGFRHPPLDGL 70

Query: 2249 VGT-PVATPKSSLPPSIAATSTQLSVANTSAYNPVKQGTPCYYFQKGLCLKGDRCPFMHG 2073
             GT  +A+  SSLPPS     T ++ A  + +NP KQ  PC +FQ+G+C+KGD+C F HG
Sbjct: 71   FGTQAIASAGSSLPPS----HTAVASATHAPHNPGKQAVPCIFFQQGICVKGDKCAFSHG 126

Query: 2072 PHPIDHVTPQPPTPKVTASVTAPQTHKKPLWVPEKCTQPKKVPQENIIIPFEVPPPARLP 1893
            P+   +   QP  P   A    P   KK     +KC Q  K+PQ  I    E P  A+  
Sbjct: 127  PNLAGNKVSQPMAPTPGAEALPP---KKAFGGLQKCAQEHKIPQTKISKAVEAPSVAKPV 183

Query: 1892 VKSVKAPLEN-GFVAEKIVPPMSLHNELPRYKPAIVXXXXXXXXXSMTNRGRLAQPVDDR 1716
             K+      N   V    VPP S+  E+PRYK A +           +   +L Q  DD 
Sbjct: 184  SKAEIVHARNIAGVVRSAVPPTSMDGEIPRYKAANLPLMVNGNLSRSSRLHQL-QVADDH 242

Query: 1715 TFHNSKEPGEFLGEASPGFDVLVDDELRNSGYYQKEDDFGRITGHAGRHLNSLNENDHSR 1536
               N K+  E+L E+SPGFDVLVD+ELR+S YY  E+  GR TG+ GR+LNS++E D   
Sbjct: 243  VIQNGKDADEYLRESSPGFDVLVDNELRDSDYYHGEEQLGRTTGNEGRNLNSVDEYDTGH 302

Query: 1535 SVDYNPPAKFDRETYSETRGYDPYRRAQDPYDWEPRRVSSERVMDRTAVPDRRALPKAES 1356
            S DY      D+E Y + RGYD Y      + WE  R SSER++    +P+RR   KA+S
Sbjct: 303  SADYG-LLHDDQEMYRDPRGYDSYELTPGQHAWEQHRASSERMLMAPVLPERRGYAKADS 361

Query: 1355 PXXXXXXXXXXXXLKQRKTNGSRSAVSPXXXXXXXXXXXXXRHVEDQRYHGHTRRDSRHL 1176
            P             KQR+ NG RS VS               HVE++ Y G +RRDS  L
Sbjct: 362  PEHTEESDLRYRLSKQRRVNGLRSVVS--------NDFVPENHVEERGYRGSSRRDSHQL 413

Query: 1175 P-HESSTSNRLQGRISLPGRSSPDRIRNDTHLEKETERGR-RARLSPGRLP--SYAGRAH 1008
            P HESS S+RL+GRI LPGRS  +    D   E+E +RGR R RLSP R P  S  GR  
Sbjct: 414  PSHESSISSRLRGRIKLPGRSPNN--GGDVRAEREIDRGRNRGRLSPVRSPVTSQQGRLR 471

Query: 1007 DRIKWGVQDEFNTEGRNFRPPLIRRDELD--NDDFAGPKSLVQLKKGTKVIERGEEQRTR 834
            DR+K  V++++N EGR+FR P +R + +D  + DFAGPKSL +LK G      G+ Q++ 
Sbjct: 472  DRMKARVEEDYN-EGRSFRGPQVRGELMDDGSTDFAGPKSLAELKGGKNA--EGKLQQSL 528

Query: 833  GKDTSFGEWKNSRIGKVXXXXXXXXXXXXXGPKPLREILKRKRRETTDVSGDGMASGDRE 654
            GK     +  +S                  GP PL EILKRKR      S  G++S  ++
Sbjct: 529  GKRKHSEDNPSS-----------DAVLSFEGPMPLSEILKRKREGGAAASRSGISSVIKD 577

Query: 653  ENNRKEQD----GDSLTVASEKQRVQPSENKEEDNDHSNSSKEEHRFATGNANGTMDDDE 486
             NN KE       +S    +E Q    S +K++++     +KEE + AT  A GT    E
Sbjct: 578  NNNLKESKQSLISNSKKADAEMQSGISSVSKDDNSSQMLKNKEESKVATDGAIGT----E 633

Query: 485  EGLISEEVEGQ-HYVQKGSEVENEDMIMLDNG-DDEELETSDQRYADSDYEQVD-GDFKT 315
            E +  E   GQ   +   SE+E ED ++ D+G +D E E  DQR  + +YEQVD G++  
Sbjct: 634  ENI--EIAHGQSSQLPNPSEIETEDGMIADDGMEDHEYEGDDQRDDNYEYEQVDEGEYNY 691

Query: 314  EEEGNAXXXXXXXXXXXXXDFAKRIGVMFS 225
            EE  N              DFAK+IGV+ S
Sbjct: 692  EEGENIDGEEEYEDEEDGEDFAKKIGVVIS 721


>ref|XP_002314739.1| predicted protein [Populus trichocarpa] gi|222863779|gb|EEF00910.1|
            predicted protein [Populus trichocarpa]
          Length = 735

 Score =  490 bits (1261), Expect = e-135
 Identities = 322/752 (42%), Positives = 418/752 (55%), Gaps = 17/752 (2%)
 Frame = -3

Query: 2429 KRNTDCVYFLASPLTCKKGVECEYRHSEEARMNPRDCWYWLNGNCLNPKCSFRHPPLDGL 2250
            KRNTDCVYFLASPLTCKKG ECEYRHSE AR+NPRDC++WLNGNC+NPKC FRHPPLDGL
Sbjct: 26   KRNTDCVYFLASPLTCKKGSECEYRHSEYARVNPRDCYFWLNGNCMNPKCGFRHPPLDGL 85

Query: 2249 VGT-PVATPKSSLPPSIAATSTQLSVANTSAYNPVKQGTPCYYFQKGLCLKGDRCPFMHG 2073
             G    A+  SSLPPS AA     + A  + +N  KQG  C +FQ+G+CLKGDRC F HG
Sbjct: 86   FGNQTTASAGSSLPPSHAAA----AAATHAPHNSGKQGVLCIFFQQGICLKGDRCAFSHG 141

Query: 2072 PHPIDHVTPQPPTPKVTASVTAPQTHKKPLWVPEKCTQPKKVPQENIIIPFEVPPPARLP 1893
            P   +H + +          T     +K     +KCTQ +K+PQ N+    E P  A+  
Sbjct: 142  P---NHASNRVSQTMAFTPGTEAHPPEKAFGGLQKCTQEQKLPQVNLSKAVEPPTVAKPV 198

Query: 1892 VKSVKAPLENGF-VAEKIVPPMSLHNELPRYKPAIVXXXXXXXXXSMTNRGRLAQPVDDR 1716
             K+  A   N   VA  +VPP S  +E+PRYK + +         S +N+  L++  DD 
Sbjct: 199  SKAEIASARNSAGVARNVVPPKSTDDEVPRYKSSNL-PPEVNGNSSRSNQLHLSRVADDH 257

Query: 1715 TFHNSKEPGEFLGEASPGFDVLVDDELRNSGYYQKEDDFGRITGHAGRHLNSLNENDHSR 1536
              HN K+  E+L E+SPGFDVLVDDELR+S YY  ED FGR  GH GR+LNS++E     
Sbjct: 258  VNHNGKDADEYLRESSPGFDVLVDDELRDSDYYHGEDQFGRTIGHEGRNLNSVDEYGRGH 317

Query: 1535 SVDYNPPAKFDRETYSETRGYDPYRRAQDPYDWEPRRVSSERVMDRTAVPDRRALPKAES 1356
              DY      D+E Y + RGYDPY   Q  + WE  R SSER++   A  +RR   KA+S
Sbjct: 318  PADYG-SLPDDQERYGDPRGYDPYEHMQGQHAWEQHRASSERMLVAPAHLERRGYSKADS 376

Query: 1355 PXXXXXXXXXXXXLKQRKTNGSRSAVSPXXXXXXXXXXXXXRHVEDQRYHGHTRRDSRH- 1179
            P             KQR+ NG RS VS               HVE++ Y G +RRDS H 
Sbjct: 377  PEHIEGSDLRYLLSKQRRVNGLRSVVS--------NDFVPGNHVEERGYQGSSRRDSHHL 428

Query: 1178 LPHESSTSNRLQGRISLPGRSSPDRIRNDTHLEKETERGR-RARLSPGR--LPSYAGRAH 1008
            L HES  S+RL+GRI LPG S  +    D H E+E +RGR R RLSP R  + S  GR  
Sbjct: 429  LSHESPISSRLRGRIKLPGGSPNE--GGDLHAEREIDRGRNRGRLSPVRSLISSQQGRFR 486

Query: 1007 DRIKWGVQDEFNTEGRNFRPPLIRRDELDN--DDFAGPKSLVQLKKGTKVIERGEEQRTR 834
            DR+K  V++++N EGRNFR P +RR+ +D+   +FA PK L +L+ G      G+ Q++ 
Sbjct: 487  DRMKARVEEDYN-EGRNFRGPHVRRELVDDKITNFAAPKRLAELRGGKNA--EGKSQQSL 543

Query: 833  GKDTSFGEWKNSRIGKVXXXXXXXXXXXXXGPKPLREILKRKRRETTDVSGDGMASGDRE 654
            GK     +   S                  GP PL EILKRKR      SG  ++S   +
Sbjct: 544  GKRKYLEDHPPS-----------DADLSFEGPMPLSEILKRKRGGGAAASGSRISSVIED 592

Query: 653  ENNRKEQDGDSLTVASEKQRVQPSEN------KEEDNDHSNSSKEEHRFATGNANGTMDD 492
             NN+KE    SLT  S  + V  +++      K++D+     +KE  +FAT +A GT   
Sbjct: 593  GNNQKE-SRQSLTSNSNNKAVAETQSGFSSVLKDDDSSQMLKNKES-KFATDDALGT--- 647

Query: 491  DEEGLISEEVEGQ-HYVQKGSEVENEDMIMLDNG-DDEELETSDQRYADSDYEQVD-GDF 321
              EG   E V G+   +   SE+E ED ++ D+G +D E E  DQR  D +YEQ D G++
Sbjct: 648  --EGGNIEIVHGKSSQLPNPSEIETEDGMIADDGMEDHEYEGDDQRDGDYEYEQADEGEY 705

Query: 320  KTEEEGNAXXXXXXXXXXXXXDFAKRIGVMFS 225
              EE  N              DFAK+IGV+FS
Sbjct: 706  NYEEGEN--IDEEEYENEDGDDFAKKIGVVFS 735


>ref|XP_003520929.1| PREDICTED: NADH-cytochrome b5 reductase 1-like [Glycine max]
          Length = 278

 Score =  390 bits (1003), Expect(2) = e-123
 Identities = 184/206 (89%), Positives = 194/206 (94%)
 Frame = +1

Query: 3112 LGLPIGQHISCRGKDALGEEVIKPYTPTTLDSDIGYFELVIKMYPQGRMSHHFREMRVGD 3291
            LGLPIGQHISCRGKDA GEEVIKPYTPTTLDSD+G+FELVIKMYPQGRMSHHFREMRVGD
Sbjct: 73   LGLPIGQHISCRGKDAQGEEVIKPYTPTTLDSDVGHFELVIKMYPQGRMSHHFREMRVGD 132

Query: 3292 YMAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMFQVARAILENPKDNTKVHLIYANVTYE 3471
            Y++VKGPKGRFKYQPG+VRAFGMLAGGSGITPMFQVARAILENP D TKVHLIYANVTYE
Sbjct: 133  YLSVKGPKGRFKYQPGEVRAFGMLAGGSGITPMFQVARAILENPNDRTKVHLIYANVTYE 192

Query: 3472 DILLKEELDSLASNYPDRFKVYYVLNQPPEEWNGGVGFVSKEMIQAHLPAPASDIRVLRC 3651
            DILLKEELD LASNYPDRFK+YYVLNQPPE W+GG GFVSKEMIQ H PAPA DI++LRC
Sbjct: 193  DILLKEELDGLASNYPDRFKIYYVLNQPPEVWDGGEGFVSKEMIQTHCPAPAQDIKILRC 252

Query: 3652 GPPPMNKAMAGHLDALEYTSEMQFQF 3729
            GPPPMNKAMA HL+AL Y SEMQFQF
Sbjct: 253  GPPPMNKAMAAHLEALGYASEMQFQF 278



 Score = 80.1 bits (196), Expect(2) = e-123
 Identities = 38/51 (74%), Positives = 43/51 (84%)
 Frame = +3

Query: 2904 YFHFSKKSKGCLNPEKFKDFKLVQRIQLSHNVAKFKFALPMTSSVLGLPIG 3056
            Y + SKK+KGCL+PE FK FKLV+R QLSHNVAKF FALP  +SVLGLPIG
Sbjct: 28   YLYSSKKTKGCLDPENFKAFKLVKRAQLSHNVAKFTFALPTPTSVLGLPIG 78


>gb|AAV69019.1| NADH:cytochrome b5 reductase [Vernicia fordii]
            gi|55979115|gb|AAV69021.1| NADH:cytochrome b5 reductase
            [Vernicia fordii]
          Length = 280

 Score =  389 bits (999), Expect(2) = e-122
 Identities = 182/206 (88%), Positives = 198/206 (96%)
 Frame = +1

Query: 3112 LGLPIGQHISCRGKDALGEEVIKPYTPTTLDSDIGYFELVIKMYPQGRMSHHFREMRVGD 3291
            LGLPIGQHISCRGKD+ GEEVIKPYTPTTLDSD+G+FELVIKMYPQGRMSHHFREMRVGD
Sbjct: 75   LGLPIGQHISCRGKDSQGEEVIKPYTPTTLDSDVGHFELVIKMYPQGRMSHHFREMRVGD 134

Query: 3292 YMAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMFQVARAILENPKDNTKVHLIYANVTYE 3471
            Y++VKGPKGRF+YQPGQVRAFGMLAGGSGITPMFQVARAILENP D TKV+LIYANVTYE
Sbjct: 135  YLSVKGPKGRFRYQPGQVRAFGMLAGGSGITPMFQVARAILENPNDKTKVYLIYANVTYE 194

Query: 3472 DILLKEELDSLASNYPDRFKVYYVLNQPPEEWNGGVGFVSKEMIQAHLPAPASDIRVLRC 3651
            DILLK+ELD LA+NYPDRFKVYYVLNQPPE W+GGVGFVSKEMI+ H PAPASDI++LRC
Sbjct: 195  DILLKQELDGLAANYPDRFKVYYVLNQPPEVWDGGVGFVSKEMIENHCPAPASDIQILRC 254

Query: 3652 GPPPMNKAMAGHLDALEYTSEMQFQF 3729
            GPPPMNKAMA HL+AL+YTS+MQFQF
Sbjct: 255  GPPPMNKAMAAHLEALDYTSDMQFQF 280



 Score = 79.7 bits (195), Expect(2) = e-122
 Identities = 38/47 (80%), Positives = 40/47 (85%)
 Frame = +3

Query: 2916 SKKSKGCLNPEKFKDFKLVQRIQLSHNVAKFKFALPMTSSVLGLPIG 3056
            SKK KGCL+PE FKDFKLV R QLSHNVAKF FALP  +SVLGLPIG
Sbjct: 34   SKKPKGCLDPENFKDFKLVNRTQLSHNVAKFSFALPTPTSVLGLPIG 80


>gb|ACU19291.1| unknown [Glycine max]
          Length = 278

 Score =  388 bits (997), Expect(2) = e-122
 Identities = 183/206 (88%), Positives = 193/206 (93%)
 Frame = +1

Query: 3112 LGLPIGQHISCRGKDALGEEVIKPYTPTTLDSDIGYFELVIKMYPQGRMSHHFREMRVGD 3291
            LGLPIGQHISCRGKDA GEEVIKPYTPTTLDSD+G+FELVIKMYPQGRMSHHFREMRVGD
Sbjct: 73   LGLPIGQHISCRGKDAQGEEVIKPYTPTTLDSDVGHFELVIKMYPQGRMSHHFREMRVGD 132

Query: 3292 YMAVKGPKGRFKYQPGQVRAFGMLAGGSGITPMFQVARAILENPKDNTKVHLIYANVTYE 3471
            Y++VKGPKGRFKYQPG+VRAFGMLAGGSGITPMFQVARAILENP D TKVHLIYANVTYE
Sbjct: 133  YLSVKGPKGRFKYQPGEVRAFGMLAGGSGITPMFQVARAILENPNDRTKVHLIYANVTYE 192

Query: 3472 DILLKEELDSLASNYPDRFKVYYVLNQPPEEWNGGVGFVSKEMIQAHLPAPASDIRVLRC 3651
            DILLKEELD LASNYPDRFK+YYVLNQPPE W+GG GFVSKEMIQ H PAPA DI++LRC
Sbjct: 193  DILLKEELDGLASNYPDRFKIYYVLNQPPEVWDGGEGFVSKEMIQTHCPAPAQDIKILRC 252

Query: 3652 GPPPMNKAMAGHLDALEYTSEMQFQF 3729
            GPPPMNKAMA HL+AL Y  EMQFQF
Sbjct: 253  GPPPMNKAMAAHLEALGYAFEMQFQF 278



 Score = 80.1 bits (196), Expect(2) = e-122
 Identities = 38/51 (74%), Positives = 43/51 (84%)
 Frame = +3

Query: 2904 YFHFSKKSKGCLNPEKFKDFKLVQRIQLSHNVAKFKFALPMTSSVLGLPIG 3056
            Y + SKK+KGCL+PE FK FKLV+R QLSHNVAKF FALP  +SVLGLPIG
Sbjct: 28   YLYSSKKTKGCLDPENFKAFKLVKRAQLSHNVAKFTFALPTPTSVLGLPIG 78