BLASTX nr result
ID: Coptis25_contig00000412
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00000412
(1702 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl... 642 0.0
gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum] 641 0.0
ref|XP_004165080.1| PREDICTED: 2-isopropylmalate synthase 2, chl... 635 e-179
ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus ... 631 e-178
ref|XP_004149446.1| PREDICTED: 2-isopropylmalate synthase 2, chl... 626 e-177
>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
vinifera]
Length = 628
Score = 642 bits (1657), Expect = 0.0
Identities = 316/397 (79%), Positives = 363/397 (91%)
Frame = +2
Query: 509 SLGCNDVEFSPEDAGRSDREYLYEVLEAVIKAGATTLNIPDTVGYTLPTEFGKLIADIKL 688
SLGC+DVEFSPEDAGRS+RE+LY++L VIKAGATTLNIPDTVGYT+P EFG+LIADIK
Sbjct: 220 SLGCDDVEFSPEDAGRSEREFLYKILGEVIKAGATTLNIPDTVGYTVPNEFGQLIADIKS 279
Query: 689 NTPGIDNVIISTHCQNDLGLSTANTIAGAYAGARQLEVTINGIGERAGNASLEEVVMAIR 868
NTPGI+NVIISTHCQNDLGLSTANTIAGA AGARQ+EVTINGIGERAGNASLEEVVMA++
Sbjct: 280 NTPGIENVIISTHCQNDLGLSTANTIAGACAGARQVEVTINGIGERAGNASLEEVVMALK 339
Query: 869 CRGKELLGGLHTGVNTRHIVMASKMVAEFTGLHVQPHKAIVGANAFAHESGIHQDGMLKF 1048
CRG++ LGGL+TG+N RHIV SKMVAE++GL VQPHKAIVGANAFAHESGIHQDGMLK
Sbjct: 340 CRGEKALGGLYTGINIRHIVKTSKMVAEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKH 399
Query: 1049 KGTYEIMSPEDIGLSRSNDSGIVLGKLSGRHALRSRLLELGYELDGKDLDDVFWRFKAVA 1228
K TYEI+SPEDIGL RSN+SGIVLGKLSGRHAL+SRLLELGYE++G +LDD+FWRFK VA
Sbjct: 400 KETYEIISPEDIGLFRSNESGIVLGKLSGRHALKSRLLELGYEIEGMELDDIFWRFKEVA 459
Query: 1229 EKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVTCGTLGLSTATVKLIGIDGVEHIACSV 1408
EKKK+L+D D+ AL+SDEIFQP+ W LGDLQVTCGTLGLSTATVKLI DG +H+ACSV
Sbjct: 460 EKKKNLTDVDVIALVSDEIFQPKVFWKLGDLQVTCGTLGLSTATVKLIDADGQKHVACSV 519
Query: 1409 GTGPVDAAYKAIDQIVKVSVSLLEYSMNSVTEGIDAIASTRVVIVGENSHTSTHAISGQT 1588
GTGPVD+AYKAID +VK V+LLEY +N+VTEGIDAIA+TRV+I GE +HT T+A++G+T
Sbjct: 520 GTGPVDSAYKAIDLLVKEQVTLLEYGLNAVTEGIDAIATTRVLIRGEKNHTLTNALTGET 579
Query: 1589 INRTFSGTGAGMDIVVSSVRAYVSALNKMFGFSRTSK 1699
++R+FSGTGAGMDIVVSSVRAY+SALNKM GF SK
Sbjct: 580 VHRSFSGTGAGMDIVVSSVRAYISALNKMLGFKDQSK 616
Score = 222 bits (565), Expect = 3e-55
Identities = 106/122 (86%), Positives = 115/122 (94%)
Frame = +1
Query: 10 SQTRPEYIPNKIPDPNYVRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIEAGF 189
S RPEYIPN+I DPNYVRIFDTTLRDGEQSPGATMTSKEKLD+ARQL+KLGVDIIEAGF
Sbjct: 69 SALRPEYIPNRIDDPNYVRIFDTTLRDGEQSPGATMTSKEKLDIARQLAKLGVDIIEAGF 128
Query: 190 PASSNDDLEAVKIIAKEVGNAIDDDGHVPVICGLARCNKADIDAAWEAVKYAKRPRIHTF 369
PA+S DDL+AVKIIAKEVGNA+D++GHVPVICGLARCN+ DI AWEAVKYAKRPRIHTF
Sbjct: 129 PAASKDDLDAVKIIAKEVGNAVDENGHVPVICGLARCNRNDISTAWEAVKYAKRPRIHTF 188
Query: 370 IA 375
IA
Sbjct: 189 IA 190
>gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum]
Length = 602
Score = 641 bits (1653), Expect = 0.0
Identities = 308/394 (78%), Positives = 365/394 (92%)
Frame = +2
Query: 509 SLGCNDVEFSPEDAGRSDREYLYEVLEAVIKAGATTLNIPDTVGYTLPTEFGKLIADIKL 688
S+GC DVEFSPEDAGRSD E+LY +L VIKAGATTLNIPDTVGYT+P EFG+LIA+IK
Sbjct: 195 SIGCQDVEFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIANIKA 254
Query: 689 NTPGIDNVIISTHCQNDLGLSTANTIAGAYAGARQLEVTINGIGERAGNASLEEVVMAIR 868
NTPG+++VIISTHCQNDLGLSTANT+AGA AGARQLEVTINGIGERAGNASLEEVVMA++
Sbjct: 255 NTPGVEDVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGERAGNASLEEVVMALK 314
Query: 869 CRGKELLGGLHTGVNTRHIVMASKMVAEFTGLHVQPHKAIVGANAFAHESGIHQDGMLKF 1048
CRG+++LGGL+TG+NT+HI+M+SKMV E+TGLHVQPHKAIVGANAFAHESGIHQDGMLK
Sbjct: 315 CRGEQVLGGLYTGINTQHILMSSKMVEEYTGLHVQPHKAIVGANAFAHESGIHQDGMLKH 374
Query: 1049 KGTYEIMSPEDIGLSRSNDSGIVLGKLSGRHALRSRLLELGYELDGKDLDDVFWRFKAVA 1228
K TYEIMSPEDIGL+R+N+SGIVLGKLSGRHAL++++LELGYE++GK+LDD+FWRFK+VA
Sbjct: 375 KDTYEIMSPEDIGLNRANESGIVLGKLSGRHALQAKMLELGYEIEGKELDDLFWRFKSVA 434
Query: 1229 EKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVTCGTLGLSTATVKLIGIDGVEHIACSV 1408
EKKK ++D D+ AL+SDE+FQPQ +W L ++QVTCG+LGLSTATVKLI DG EHI+CSV
Sbjct: 435 EKKKKITDDDLIALMSDEVFQPQFVWQLQNVQVTCGSLGLSTATVKLIDADGKEHISCSV 494
Query: 1409 GTGPVDAAYKAIDQIVKVSVSLLEYSMNSVTEGIDAIASTRVVIVGENSHTSTHAISGQT 1588
GTGPVDAAYKA+D IVK+ V+LLEYSMN+VT+GIDAIASTRV+I GEN HTSTHA++G+T
Sbjct: 495 GTGPVDAAYKAVDLIVKIPVTLLEYSMNAVTQGIDAIASTRVLIRGENGHTSTHALTGET 554
Query: 1589 INRTFSGTGAGMDIVVSSVRAYVSALNKMFGFSR 1690
++RTFSGTGA MDIV+SSVRAY+ ALNKM F +
Sbjct: 555 VHRTFSGTGADMDIVISSVRAYIGALNKMMSFRK 588
Score = 212 bits (540), Expect = 2e-52
Identities = 103/125 (82%), Positives = 114/125 (91%)
Frame = +1
Query: 1 CSLSQTRPEYIPNKIPDPNYVRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIE 180
CS+ + RPEY P+ I DPNY+RIFDTTLRDGEQSPGATMT+KEKLDVARQL+KLGVDIIE
Sbjct: 42 CSI-RCRPEYTPSHISDPNYIRIFDTTLRDGEQSPGATMTTKEKLDVARQLAKLGVDIIE 100
Query: 181 AGFPASSNDDLEAVKIIAKEVGNAIDDDGHVPVICGLARCNKADIDAAWEAVKYAKRPRI 360
AGFPASS DLEAVK+IAKEVGN + ++GHVPVICGLARCNK DID AWEAVKYAK+PRI
Sbjct: 101 AGFPASSEADLEAVKLIAKEVGNGVYEEGHVPVICGLARCNKRDIDKAWEAVKYAKKPRI 160
Query: 361 HTFIA 375
HTFIA
Sbjct: 161 HTFIA 165
>ref|XP_004165080.1| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis
sativus]
Length = 639
Score = 635 bits (1637), Expect = e-179
Identities = 309/392 (78%), Positives = 357/392 (91%)
Frame = +2
Query: 509 SLGCNDVEFSPEDAGRSDREYLYEVLEAVIKAGATTLNIPDTVGYTLPTEFGKLIADIKL 688
SLGC+DVEFSPEDAGRSDRE+LY++L VIKAGATTLNIPDTVGYT+P EFGKLIADIK
Sbjct: 227 SLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKS 286
Query: 689 NTPGIDNVIISTHCQNDLGLSTANTIAGAYAGARQLEVTINGIGERAGNASLEEVVMAIR 868
NTPGI+NVIIS+HCQNDLGL+TANT+AGA AGARQ+EVTINGIGERAGNASLEEVVMA++
Sbjct: 287 NTPGIENVIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQ 346
Query: 869 CRGKELLGGLHTGVNTRHIVMASKMVAEFTGLHVQPHKAIVGANAFAHESGIHQDGMLKF 1048
CRG+ +LGGLHTG+N+RHI + SKMV EFTGL+VQPHKAIVGANAFAH SGIHQDGMLK
Sbjct: 347 CRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKH 406
Query: 1049 KGTYEIMSPEDIGLSRSNDSGIVLGKLSGRHALRSRLLELGYELDGKDLDDVFWRFKAVA 1228
KGTYEIM+PEDIG RSND+GIVLGKLSGRHAL+S LLELGYELDG++LD+VFWRFKAVA
Sbjct: 407 KGTYEIMAPEDIGYERSNDAGIVLGKLSGRHALKSLLLELGYELDGENLDNVFWRFKAVA 466
Query: 1229 EKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVTCGTLGLSTATVKLIGIDGVEHIACSV 1408
E+KK ++D D+ AL+SDE+FQP +W L D+QVTCGTLGLSTATVKL+ DG EHIACSV
Sbjct: 467 EQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSV 526
Query: 1409 GTGPVDAAYKAIDQIVKVSVSLLEYSMNSVTEGIDAIASTRVVIVGENSHTSTHAISGQT 1588
GTGPVD+AYKA+D IVK +LLEYSMN+VTEGIDAIA+TRV+I G+ S+TST+A++G+
Sbjct: 527 GTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEA 586
Query: 1589 INRTFSGTGAGMDIVVSSVRAYVSALNKMFGF 1684
+ RTFSG GAGMDIVVSSV+AY+ ALNKM GF
Sbjct: 587 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 618
Score = 209 bits (531), Expect = 2e-51
Identities = 96/119 (80%), Positives = 112/119 (94%)
Frame = +1
Query: 19 RPEYIPNKIPDPNYVRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIEAGFPAS 198
RP YIPN+IPDP+YVR+FDTTLRDGEQSPGA++T KEKLD+ARQL+KLGVDIIEAGFPA+
Sbjct: 79 RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAA 138
Query: 199 SNDDLEAVKIIAKEVGNAIDDDGHVPVICGLARCNKADIDAAWEAVKYAKRPRIHTFIA 375
S +D EAVK+IAKE+GNA+D+DG+VPVICGL+RCN+ DI AWEAVKYAKRPRIHTFIA
Sbjct: 139 SKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIA 197
>ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus communis]
gi|223529911|gb|EEF31840.1| isopropylmalate synthase,
putative [Ricinus communis]
Length = 632
Score = 631 bits (1628), Expect = e-178
Identities = 311/394 (78%), Positives = 351/394 (89%)
Frame = +2
Query: 509 SLGCNDVEFSPEDAGRSDREYLYEVLEAVIKAGATTLNIPDTVGYTLPTEFGKLIADIKL 688
SLGC+DVEFSPEDAGRSDRE+LY +L VIKAGATTLNIPDTVG TLP+EFG+LIADIK
Sbjct: 222 SLGCDDVEFSPEDAGRSDREFLYHILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKA 281
Query: 689 NTPGIDNVIISTHCQNDLGLSTANTIAGAYAGARQLEVTINGIGERAGNASLEEVVMAIR 868
NTPGID VIISTHCQNDLGLSTANT+AGAYAGARQ+EVTINGIGERAGNASLEEVVMAI+
Sbjct: 282 NTPGIDKVIISTHCQNDLGLSTANTLAGAYAGARQVEVTINGIGERAGNASLEEVVMAIK 341
Query: 869 CRGKELLGGLHTGVNTRHIVMASKMVAEFTGLHVQPHKAIVGANAFAHESGIHQDGMLKF 1048
CRG+ +LGGL+TG+N R I MASKMV E+TGLH+QPHKAIVGANAFAHESGIHQDGMLK
Sbjct: 342 CRGEHILGGLYTGINPRQITMASKMVEEYTGLHLQPHKAIVGANAFAHESGIHQDGMLKH 401
Query: 1049 KGTYEIMSPEDIGLSRSNDSGIVLGKLSGRHALRSRLLELGYELDGKDLDDVFWRFKAVA 1228
KGTYEI+SPEDIGL RSND+GIVLGKLSGRHAL+ RL ELGYEL+ + L +FWRFKAVA
Sbjct: 402 KGTYEIISPEDIGLERSNDAGIVLGKLSGRHALKDRLKELGYELNDEQLGTIFWRFKAVA 461
Query: 1229 EKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVTCGTLGLSTATVKLIGIDGVEHIACSV 1408
E KK ++D D+ AL+SDE+FQP+ +W LGDLQVTCGTLGLSTATVKLI DG EHIACSV
Sbjct: 462 ETKKRVTDADLIALVSDEVFQPEIVWKLGDLQVTCGTLGLSTATVKLINADGEEHIACSV 521
Query: 1409 GTGPVDAAYKAIDQIVKVSVSLLEYSMNSVTEGIDAIASTRVVIVGENSHTSTHAISGQT 1588
G+GPVDA YKA+D IVK V LLEYSMN+VTEGIDAIA+TRV+I E++HTSTHA++G+
Sbjct: 522 GSGPVDAGYKAVDLIVKEPVELLEYSMNAVTEGIDAIATTRVLIRREDNHTSTHALTGEP 581
Query: 1589 INRTFSGTGAGMDIVVSSVRAYVSALNKMFGFSR 1690
RTFSG+GAGMDIVVSSV+AYV ALNKM +
Sbjct: 582 FQRTFSGSGAGMDIVVSSVKAYVGALNKMLALKQ 615
Score = 214 bits (546), Expect = 4e-53
Identities = 102/119 (85%), Positives = 112/119 (94%)
Frame = +1
Query: 19 RPEYIPNKIPDPNYVRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIEAGFPAS 198
RPEYIP+ IPDP+YVRIFDTTLRDGEQSPGAT+TSKEKLD+ARQLSKLGVDIIEAGFPA+
Sbjct: 74 RPEYIPSHIPDPSYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLSKLGVDIIEAGFPAA 133
Query: 199 SNDDLEAVKIIAKEVGNAIDDDGHVPVICGLARCNKADIDAAWEAVKYAKRPRIHTFIA 375
S DD EAVK IAKEVGNA+D+DG+VPVICGL+RCN+ DI AWEAVKYAKRPRIHTFIA
Sbjct: 134 SKDDFEAVKTIAKEVGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIA 192
>ref|XP_004149446.1| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Cucumis
sativus]
Length = 633
Score = 626 bits (1615), Expect = e-177
Identities = 305/392 (77%), Positives = 354/392 (90%)
Frame = +2
Query: 509 SLGCNDVEFSPEDAGRSDREYLYEVLEAVIKAGATTLNIPDTVGYTLPTEFGKLIADIKL 688
SLGC+DVEFSPEDAGRSDRE+LY++L VIKAGATTLNIPDTVGYT+P EFGKLIADIK
Sbjct: 227 SLGCDDVEFSPEDAGRSDREFLYQILGEVIKAGATTLNIPDTVGYTMPFEFGKLIADIKS 286
Query: 689 NTPGIDNVIISTHCQNDLGLSTANTIAGAYAGARQLEVTINGIGERAGNASLEEVVMAIR 868
NTPGI+NVIIS+HCQNDLGL+TANT+AGA AGARQ+EVTINGIGERAGNASLEEVVMA++
Sbjct: 287 NTPGIENVIISSHCQNDLGLATANTVAGACAGARQVEVTINGIGERAGNASLEEVVMALQ 346
Query: 869 CRGKELLGGLHTGVNTRHIVMASKMVAEFTGLHVQPHKAIVGANAFAHESGIHQDGMLKF 1048
CRG+ +LGGLHTG+N+RHI + SKMV EFTGL+VQPHKAIVGANAFAH SGIHQDGMLK
Sbjct: 347 CRGEHVLGGLHTGINSRHIFLTSKMVEEFTGLNVQPHKAIVGANAFAHASGIHQDGMLKH 406
Query: 1049 KGTYEIMSPEDIGLSRSNDSGIVLGKLSGRHALRSRLLELGYELDGKDLDDVFWRFKAVA 1228
KGTYEIM+PEDIG RSND+GIV + SGRHAL+S LLELGYELDG++LD+VFWRFKAVA
Sbjct: 407 KGTYEIMAPEDIGYERSNDAGIVFFEYSGRHALKSLLLELGYELDGENLDNVFWRFKAVA 466
Query: 1229 EKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVTCGTLGLSTATVKLIGIDGVEHIACSV 1408
E+KK ++D D+ AL+SDE+FQP +W L D+QVTCGTLGLSTATVKL+ DG EHIACSV
Sbjct: 467 EQKKRVTDADLRALVSDEVFQPTVLWKLLDMQVTCGTLGLSTATVKLLDADGKEHIACSV 526
Query: 1409 GTGPVDAAYKAIDQIVKVSVSLLEYSMNSVTEGIDAIASTRVVIVGENSHTSTHAISGQT 1588
GTGPVD+AYKA+D IVK +LLEYSMN+VTEGIDAIA+TRV+I G+ S+TST+A++G+
Sbjct: 527 GTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLIRGDKSYTSTNALTGEA 586
Query: 1589 INRTFSGTGAGMDIVVSSVRAYVSALNKMFGF 1684
+ RTFSG GAGMDIVVSSV+AY+ ALNKM GF
Sbjct: 587 VQRTFSGIGAGMDIVVSSVKAYIGALNKMLGF 618
Score = 209 bits (531), Expect = 2e-51
Identities = 96/119 (80%), Positives = 112/119 (94%)
Frame = +1
Query: 19 RPEYIPNKIPDPNYVRIFDTTLRDGEQSPGATMTSKEKLDVARQLSKLGVDIIEAGFPAS 198
RP YIPN+IPDP+YVR+FDTTLRDGEQSPGA++T KEKLD+ARQL+KLGVDIIEAGFPA+
Sbjct: 79 RPPYIPNRIPDPSYVRVFDTTLRDGEQSPGASLTVKEKLDIARQLAKLGVDIIEAGFPAA 138
Query: 199 SNDDLEAVKIIAKEVGNAIDDDGHVPVICGLARCNKADIDAAWEAVKYAKRPRIHTFIA 375
S +D EAVK+IAKE+GNA+D+DG+VPVICGL+RCN+ DI AWEAVKYAKRPRIHTFIA
Sbjct: 139 SKEDFEAVKMIAKEIGNAVDEDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIA 197