BLASTX nr result
ID: Coptis25_contig00000237
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00000237
(2471 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta] 952 0.0
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 583 e-164
ref|XP_002282035.1| PREDICTED: cytochrome P450 82A3-like [Vitis ... 583 e-164
ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 583 e-164
ref|XP_002282085.2| PREDICTED: cytochrome P450 82A3-like [Vitis ... 568 e-159
>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 511
Score = 952 bits (2461), Expect = 0.0
Identities = 472/510 (92%), Positives = 479/510 (93%)
Frame = -1
Query: 2396 FISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI 2217
FISLYFLILWR SSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI
Sbjct: 2 FISLYFLILWRTRSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI 61
Query: 2216 FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE 2037
FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE
Sbjct: 62 FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE 121
Query: 2036 LRKITMXXXXXXXXXXXLKHVRDSEINTSIQELYKVWKNHDKAKGPVLVDMKQWFGDLTL 1857
LRKITM LKHVRDSEI+ SIQ+LYKVWKNHDKAKGPVLVDMKQWFGDLTL
Sbjct: 122 LRKITMSELLSNRRLELLKHVRDSEIDASIQDLYKVWKNHDKAKGPVLVDMKQWFGDLTL 181
Query: 1856 NVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWLDLQGYKK 1677
NVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSW DLQGYKK
Sbjct: 182 NVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWFDLQGYKK 241
Query: 1676 EMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSILEDTKISEYDNDTIIKST 1497
EMKNTAKELDSVFQRWLEEH RMRQTGELNREQDFMDVLMSILEDT+ISEYDNDTIIKST
Sbjct: 242 EMKNTAKELDSVFQRWLEEHNRMRQTGELNREQDFMDVLMSILEDTRISEYDNDTIIKST 301
Query: 1496 CLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVGKDRHVEESDIKNLIYLQA 1317
CLSIVTGG DTTMVTLTWI AQDELDSHVGKDR VEESDIKNL+YLQA
Sbjct: 302 CLSIVTGGGDTTMVTLTWILSLLLNNKHALKKAQDELDSHVGKDRQVEESDIKNLVYLQA 361
Query: 1316 ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE 1137
ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE
Sbjct: 362 ITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPE 421
Query: 1136 RFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVD 957
RFLTSHVNMD+KGLHYELIPFGAGRR+CPGMSFTLQVVPLVLARFLHEF+SKTEMDMPVD
Sbjct: 422 RFLTSHVNMDVKGLHYELIPFGAGRRSCPGMSFTLQVVPLVLARFLHEFDSKTEMDMPVD 481
Query: 956 MTETAGLTNAKATPLEVVITPRLHPEIYGL 867
MTETAGLTNAKATPLEVVITPRLHPEIYGL
Sbjct: 482 MTETAGLTNAKATPLEVVITPRLHPEIYGL 511
>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|302142392|emb|CBI19595.3| unnamed protein product
[Vitis vinifera]
Length = 527
Score = 583 bits (1503), Expect = e-164
Identities = 291/526 (55%), Positives = 384/526 (73%), Gaps = 10/526 (1%)
Frame = -1
Query: 2420 PMITLLVFFISL----YFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSE-LRH 2256
P + L FF+ L F + R K++ ++APE GAWPIIGHLHLLGG + L +
Sbjct: 3 PALHLPAFFVFLSLIYVFYAMLGRKKIIKSSKSRDAPEPGGAWPIIGHLHLLGGGDQLLY 62
Query: 2255 KTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYV 2076
+TLGAMADKYGP F IR+G A VVS+ ++AKECFT NDKA A+RPT+ A+K +GY+Y
Sbjct: 63 RTLGAMADKYGPAFNIRLGSRRAFVVSSWEVAKECFTINDKALATRPTTVAAKHMGYNYA 122
Query: 2075 MFGMAPYGQYWVELRKITMXXXXXXXXXXXLKHVRDSEINTSIQELYKVW-KNHDKAKGP 1899
+FG APY +W E+RKI LKHVR SE++ I+ELY +W KN + P
Sbjct: 123 VFGFAPYSPFWREMRKIATLELLSNRRLEILKHVRTSEVDMGIRELYGLWVKNSSR---P 179
Query: 1898 VLVDMKQWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDA 1719
+LV++ +W D+TLNVI+RM+AGKRY G+ ++ D +EAR CQK + FFRL+G+F++ DA
Sbjct: 180 LLVELNRWLEDMTLNVIVRMVAGKRYFGAAAASDSSEARRCQKAISQFFRLIGIFVVSDA 239
Query: 1718 LPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNRE--QDFMDVLMSILE 1545
LP+L WLDLQG+++ MK TAKELDS+ + WLEEH++ R + + E QDF+DV++S+ E
Sbjct: 240 LPFLWWLDLQGHERAMKTTAKELDSILEGWLEEHRQRRVSSLIKAEGEQDFIDVMLSLQE 299
Query: 1544 DTKIS--EYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVG 1371
+ ++S +YD++T IKSTCL+++ GGSDTT TLTW AQ+ELD VG
Sbjct: 300 EGRLSGFQYDSETSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHALKKAQEELDLCVG 359
Query: 1370 KDRHVEESDIKNLIYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTY 1191
+R VEESD+KNL+YLQAI KE LRLYPAGPL GPR A DCTVAGY+VPAGTRLIVN +
Sbjct: 360 MERQVEESDVKNLVYLQAIIKETLRLYPAGPLLGPREALDDCTVAGYNVPAGTRLIVNIW 419
Query: 1190 KIQRDPLVWSEPSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVL 1011
K+QRDP VW+ P F+PERFL +H ++D+KG +EL+PFG+GRR+CPG+SF LQV+ L L
Sbjct: 420 KLQRDPSVWTNPCAFQPERFLNAHADVDVKGQQFELMPFGSGRRSCPGVSFALQVLHLTL 479
Query: 1010 ARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHPEIY 873
AR LH FE T +D PVDMTE++GLT KATPLEV++TPRL+ ++Y
Sbjct: 480 ARLLHAFELSTPVDQPVDMTESSGLTIPKATPLEVLLTPRLNSKLY 525
>ref|XP_002282035.1| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 528
Score = 583 bits (1503), Expect = e-164
Identities = 290/517 (56%), Positives = 367/517 (70%), Gaps = 1/517 (0%)
Frame = -1
Query: 2435 QYLPTPMITLL-VFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELR 2259
QYL I +L + F+S Y L+ RR S+ + APEA GAWPIIGHLHLLGGS+L
Sbjct: 11 QYLNITTIGVLGILFLSYYLLV--RR---SRAGKRRIAPEAAGAWPIIGHLHLLGGSQLP 65
Query: 2258 HKTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDY 2079
H TLG MADKYGPIF IR+GV+ ALVVS+ ++AKECFTTND A + RP A + LGY+Y
Sbjct: 66 HVTLGTMADKYGPIFTIRLGVHRALVVSSREVAKECFTTNDSAVSGRPKLVAPEHLGYNY 125
Query: 2078 VMFGMAPYGQYWVELRKITMXXXXXXXXXXXLKHVRDSEINTSIQELYKVWKNHDKAKGP 1899
MF +PY YW E+RKI LK VR SE+ TSI+ELYK+W G
Sbjct: 126 AMFAFSPYDAYWREVRKIVNTELLSNRRLELLKDVRASEVETSIKELYKLWAEKKNELGH 185
Query: 1898 VLVDMKQWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDA 1719
VLV+MKQWFGDL++NVILRM+ GKRY G + +E EAR CQK +R+FFRLLGLF+++D
Sbjct: 186 VLVEMKQWFGDLSMNVILRMVVGKRYFGVGAGGEEEEARRCQKAIREFFRLLGLFVVKDG 245
Query: 1718 LPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSILEDT 1539
+P L WLDL G++K MK TAKE+DS+ Q WLEEH+R + GE N DFMDVL+S+L+
Sbjct: 246 IPSLGWLDLGGHEKAMKKTAKEIDSIAQEWLEEHRRRKDWGEDNGMHDFMDVLLSVLDGK 305
Query: 1538 KISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVGKDRH 1359
+ EYD DTI K+T +++++GG+DT VTLTW A++ELD+HVGK+R
Sbjct: 306 ALPEYDADTINKATSMALISGGTDTMTVTLTWALSLILNNRETLKKAKEELDTHVGKERL 365
Query: 1358 VEESDIKNLIYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQR 1179
V SDI L+YLQAI KE LRL P GPLSGPR DC + GYHVP GTRL++N K+ R
Sbjct: 366 VNASDISKLVYLQAIVKETLRLRPPGPLSGPRQFTEDCIIGGYHVPKGTRLVLNLSKLHR 425
Query: 1178 DPLVWSEPSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFL 999
DP VW +P EF+PERFLT+H ++D +G H++L+PFGAGRR+CPG++F LQ++ L LA FL
Sbjct: 426 DPSVWLDPEEFQPERFLTTHRDVDARGQHFQLLPFGAGRRSCPGITFALQMLHLALASFL 485
Query: 998 HEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRL 888
H FE T + PVDM+E GLTN K+TPLE++I PRL
Sbjct: 486 HGFEVSTPSNAPVDMSEIPGLTNIKSTPLEILIAPRL 522
>ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|147794787|emb|CAN66846.1| hypothetical protein
VITISV_002367 [Vitis vinifera]
Length = 528
Score = 583 bits (1502), Expect = e-164
Identities = 289/515 (56%), Positives = 369/515 (71%), Gaps = 2/515 (0%)
Frame = -1
Query: 2411 TLLVFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMAD 2232
+LLV IS+ F L + ++S C AP A GAWPIIGHLHL G +L HKTLGAMAD
Sbjct: 14 SLLVVVISICFYRLKIK-AASNAKRCT-APRAGGAWPIIGHLHLFGAQQLTHKTLGAMAD 71
Query: 2231 KYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYG 2052
KYGP+F IR+G+N LV+S+S++A+ECFTT+D+ F++RP+ TASKILGYD+ MFG APYG
Sbjct: 72 KYGPVFTIRLGLNEILVLSSSEMARECFTTHDRVFSTRPSVTASKILGYDFAMFGFAPYG 131
Query: 2051 QYWVELRKITMXXXXXXXXXXXLKHVRDSEINTSIQELYKVWKNHDKAKGPVLVDMKQWF 1872
YW E+RKI LKH+R SE+ TSI+ELY++W + G V VDMK+WF
Sbjct: 132 SYWREMRKIVTIELLSNHRLDMLKHIRASEVGTSIRELYEMWVSERGTDGRVFVDMKRWF 191
Query: 1871 GDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSWLDL 1692
GDLTLN+ +R++ GKRY G+ + E E RTCQK +RDF L G+F++ DA+P+LSWLDL
Sbjct: 192 GDLTLNLAVRLVGGKRYFGAGADTKEGEGRTCQKVIRDFAHLFGVFVLSDAIPFLSWLDL 251
Query: 1691 QGYKKEMKNTAKELDSVFQRWLEEHKRMRQT-GELNREQDFMDVLMSILEDTKISEYDND 1515
+GYKK MK TAKELDS+F WL+EHK R GE +QDFMDV++++LED S +D D
Sbjct: 252 KGYKKAMKRTAKELDSLFGGWLQEHKEKRLLGGEGKDDQDFMDVMLTVLEDVNFSGFDAD 311
Query: 1514 TIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVGKDRHVEESDIKN 1335
T+ K+TCL+++ GSDTT VTLTW AQ ELD VGKDR V+ESD+KN
Sbjct: 312 TVNKATCLNLILAGSDTTKVTLTWALSLLLNHPHVLKKAQAELDIQVGKDRQVDESDVKN 371
Query: 1334 LIYLQAITKEALRLYPAGPLSGPRVADADCTV-AGYHVPAGTRLIVNTYKIQRDPLVWSE 1158
L+YLQAI KE LRLYPA P+ A DCT+ AGY++ AGT+++VN +KI RD VW
Sbjct: 372 LVYLQAIIKETLRLYPASPIITLHAAMEDCTLAAGYNISAGTQIMVNAWKIHRDERVWCN 431
Query: 1157 PSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFLHEFESKT 978
P EF+PERF+TSH + D++G H+ELIPFG+GRR+CPG+S LQVV LA LH +E
Sbjct: 432 PKEFQPERFMTSHKDTDVRGQHFELIPFGSGRRSCPGISLALQVVHFALASLLHSYEVTK 491
Query: 977 EMDMPVDMTETAGLTNAKATPLEVVITPRLHPEIY 873
D VDMTE+ GLTN KATPLEV+++PRL E+Y
Sbjct: 492 PSDGDVDMTESLGLTNLKATPLEVLLSPRLKAELY 526
>ref|XP_002282085.2| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 514
Score = 568 bits (1464), Expect = e-159
Identities = 280/511 (54%), Positives = 357/511 (69%), Gaps = 1/511 (0%)
Frame = -1
Query: 2417 MITLLVFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSE-LRHKTLGA 2241
M+ + Y L+LWR S + APEA GAWPIIGHLHLLGGS+ L H G
Sbjct: 1 MVGAFAILVLSYHLLLWR----SGAGKSRMAPEASGAWPIIGHLHLLGGSKNLPHLLFGT 56
Query: 2240 MADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMA 2061
MADKYGP+F IR+G+ A+VVS+ ++AKECFTT+D A ASRP A+K LGY+Y MF +
Sbjct: 57 MADKYGPVFSIRLGLKRAVVVSSWEMAKECFTTHDLALASRPELVAAKYLGYNYAMFAFS 116
Query: 2060 PYGQYWVELRKITMXXXXXXXXXXXLKHVRDSEINTSIQELYKVWKNHDKAKGPVLVDMK 1881
P+G YW E+RKI LK+VR SE+ T ++ELYK+W G VLVDMK
Sbjct: 117 PHGAYWREVRKIATLELLSNRRLELLKNVRISEVETCMKELYKLWAEKKSEAGVVLVDMK 176
Query: 1880 QWFGDLTLNVILRMIAGKRYSGSMSSCDETEARTCQKGMRDFFRLLGLFIIEDALPYLSW 1701
QWFG L+LNVIL+M+ GKRY G + E EA+ CQK +R+FFRLLGLF++ DALP+L W
Sbjct: 177 QWFGHLSLNVILKMVVGKRYFGYAAESKEKEAQQCQKAIREFFRLLGLFVVSDALPFLGW 236
Query: 1700 LDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSILEDTKISEYD 1521
LD+ G+ K MK TAKELD + Q WLEEH+R + +GE + +QDFMDV++SIL T + YD
Sbjct: 237 LDVGGHVKAMKKTAKELDGIAQEWLEEHRRRKDSGEADGDQDFMDVMLSILGATDPNGYD 296
Query: 1520 NDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVGKDRHVEESDI 1341
DTI K+T L ++ GG+DTT VTLTW AQ+ELD+HVGK+R V E DI
Sbjct: 297 ADTINKATSLILIAGGTDTTSVTLTWAISLLLNNPHVLRKAQEELDTHVGKERLVNEMDI 356
Query: 1340 KNLIYLQAITKEALRLYPAGPLSGPRVADADCTVAGYHVPAGTRLIVNTYKIQRDPLVWS 1161
L+YLQAI KE LRLYPA PLSG R D + GYH+P GTRL++N KIQRDP VW
Sbjct: 357 SKLVYLQAIVKETLRLYPAAPLSGQRQFIQDSVLGGYHIPKGTRLLLNLTKIQRDPRVWL 416
Query: 1160 EPSEFRPERFLTSHVNMDIKGLHYELIPFGAGRRACPGMSFTLQVVPLVLARFLHEFESK 981
P++F+P RFLT++ ++D+KG H+ L PFG GRR CPG +F LQV+PL LA FLH+F+
Sbjct: 417 NPTKFQPSRFLTTYKDVDVKGKHFVLTPFGGGRRICPGAAFALQVLPLTLANFLHKFQLS 476
Query: 980 TEMDMPVDMTETAGLTNAKATPLEVVITPRL 888
T + P+DM+E+ G+TN K+TPLEV+I+PRL
Sbjct: 477 TPSNSPIDMSESFGITNIKSTPLEVLISPRL 507