BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00000213
         (1504 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002531873.1| methylenetetrahydrofolate dehydrogenase, put...   374   e-109
ref|XP_002263128.2| PREDICTED: c-1-tetrahydrofolate synthase, cy...   366   e-109
ref|XP_004152261.1| PREDICTED: c-1-tetrahydrofolate synthase, cy...   365   e-108
gb|ABK96126.1| unknown [Populus trichocarpa]                          367   e-108
gb|AAD01907.1| 5,10-methylenetetrahydrofolate dehydrogenase-5,10...   360   e-106

>ref|XP_002531873.1| methylenetetrahydrofolate dehydrogenase, putative [Ricinus
           communis] gi|223528481|gb|EEF30510.1|
           methylenetetrahydrofolate dehydrogenase, putative
           [Ricinus communis]
          Length = 299

 Score =  374 bits (960), Expect(2) = e-109
 Identities = 190/239 (79%), Positives = 207/239 (86%)
 Frame = +1

Query: 94  KATIIDGKAIAHTIRSEIASEVQQLSTKHGKVPGLAVIIVGSRKDSQSYVNMKRKACAEV 273
           KATIIDGKAIA TIRSEIA EV+QLS K+GKVPGLAV+IVG+RKDSQSYV+MKRKACAEV
Sbjct: 8   KATIIDGKAIAQTIRSEIADEVRQLSEKYGKVPGLAVVIVGNRKDSQSYVSMKRKACAEV 67

Query: 274 GIKSYDINLPEKVPEAELIQKVQELNAHPDIHGILVQLPLPAHINEENVLNEISIEKDVD 453
           GIKS+DINLPE+V EAELI KV ELNA+ D+HGILVQLPLP HINEE VL+EISIEKDVD
Sbjct: 68  GIKSFDINLPEQVSEAELISKVHELNANTDVHGILVQLPLPKHINEEKVLSEISIEKDVD 127

Query: 454 GFHPLNIGKLGMKGRDPLFQPCTPKGCLELLSRSGISVKSKRAVIVGRSNIVGLPVALLL 633
           GFHPLNIGKL MKGR+PLF PCTPKGCLELLSRSGIS+K K+AV+VGRSNIVGLPV+LLL
Sbjct: 128 GFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISIKGKKAVVVGRSNIVGLPVSLLL 187

Query: 634 LKADATVTVVHSRTTXXXXXXXXXXXXXXXXGQANMIKGDWIKPGAAVIDVGTNAVDDP 810
           LKADATV++VHSRT                 GQ  MIKG WIKPGAAVIDVGTNAVDDP
Sbjct: 188 LKADATVSIVHSRTEDPERIICEADIIIAAAGQPMMIKGSWIKPGAAVIDVGTNAVDDP 246



 Score = 49.7 bits (117), Expect(2) = e-109
 Identities = 25/52 (48%), Positives = 30/52 (57%)
 Frame = +2

Query: 902  RKSGYRLVGDVDFEQACKXXXXXXXXXXXXXXXXXXXLLKNTLDGAKRKIEQ 1057
            +KSGYRLVGDVD+++AC                    LLKNTLDGAKR+  Q
Sbjct: 248  KKSGYRLVGDVDYKEACNVAGWITPVPGGVGPMTVAMLLKNTLDGAKREFMQ 299


>ref|XP_002263128.2| PREDICTED: c-1-tetrahydrofolate synthase, cytoplasmic [Vitis
           vinifera]
          Length = 307

 Score =  366 bits (939), Expect(2) = e-109
 Identities = 187/239 (78%), Positives = 205/239 (85%)
 Frame = +1

Query: 94  KATIIDGKAIAHTIRSEIASEVQQLSTKHGKVPGLAVIIVGSRKDSQSYVNMKRKACAEV 273
           KA+IIDGKAIA  IRSEIA EV+ LS K+GKVPGLAV+IVG+RKDSQSYV+MKRKACAEV
Sbjct: 8   KASIIDGKAIAQAIRSEIAEEVRHLSEKYGKVPGLAVVIVGNRKDSQSYVSMKRKACAEV 67

Query: 274 GIKSYDINLPEKVPEAELIQKVQELNAHPDIHGILVQLPLPAHINEENVLNEISIEKDVD 453
           GIKS+D++LPE+V E+ELI KV ELNA PD+HGILVQLPLP HINEE VL+EIS+EKDVD
Sbjct: 68  GIKSFDVDLPEQVLESELIGKVHELNALPDVHGILVQLPLPKHINEEKVLSEISLEKDVD 127

Query: 454 GFHPLNIGKLGMKGRDPLFQPCTPKGCLELLSRSGISVKSKRAVIVGRSNIVGLPVALLL 633
           GFHPLNIGKL MKGR+PLF PCTPKGCLELLSRSGISVK K+AV+VGRSNIVGLPVALLL
Sbjct: 128 GFHPLNIGKLAMKGREPLFLPCTPKGCLELLSRSGISVKGKKAVVVGRSNIVGLPVALLL 187

Query: 634 LKADATVTVVHSRTTXXXXXXXXXXXXXXXXGQANMIKGDWIKPGAAVIDVGTNAVDDP 810
           LKADATVTVVHS T                 GQA MIKG WIKPGAAVIDVGTNAV+DP
Sbjct: 188 LKADATVTVVHSHTQDPESIIRDADIVIAAAGQAMMIKGSWIKPGAAVIDVGTNAVNDP 246



 Score = 56.2 bits (134), Expect(2) = e-109
 Identities = 29/52 (55%), Positives = 32/52 (61%)
 Frame = +2

Query: 902  RKSGYRLVGDVDFEQACKXXXXXXXXXXXXXXXXXXXLLKNTLDGAKRKIEQ 1057
            +KSGYRLVGDVDF++ACK                   LLKNTLDGAKR IEQ
Sbjct: 248  KKSGYRLVGDVDFQEACKVAGWVTPVPGGVGPMTVAMLLKNTLDGAKRVIEQ 299


>ref|XP_004152261.1| PREDICTED: c-1-tetrahydrofolate synthase, cytoplasmic-like [Cucumis
           sativus] gi|449484305|ref|XP_004156845.1| PREDICTED:
           c-1-tetrahydrofolate synthase, cytoplasmic-like [Cucumis
           sativus]
          Length = 299

 Score =  365 bits (937), Expect(2) = e-108
 Identities = 181/245 (73%), Positives = 206/245 (84%)
 Frame = +1

Query: 76  SEEEQKKATIIDGKAIAHTIRSEIASEVQQLSTKHGKVPGLAVIIVGSRKDSQSYVNMKR 255
           + E   KATIIDGK IA T+RSEI  EV +LS K+GK+PGLAV+IVG+RKDS +YVNMKR
Sbjct: 2   ASESDHKATIIDGKKIAQTVRSEITEEVNKLSQKYGKIPGLAVVIVGNRKDSLTYVNMKR 61

Query: 256 KACAEVGIKSYDINLPEKVPEAELIQKVQELNAHPDIHGILVQLPLPAHINEENVLNEIS 435
           KAC EVGIKS++I+LPE+V EAELI KV ELNA+P++HGILVQLPLP HINEE VL+EIS
Sbjct: 62  KACLEVGIKSFEIDLPEQVSEAELISKVHELNANPEVHGILVQLPLPNHINEEKVLSEIS 121

Query: 436 IEKDVDGFHPLNIGKLGMKGRDPLFQPCTPKGCLELLSRSGISVKSKRAVIVGRSNIVGL 615
           IEKDVDGFHPLNIGKL MKGRDPLF PCTPKGC+ELLSRSGIS++ K+AV++GRSNIVGL
Sbjct: 122 IEKDVDGFHPLNIGKLAMKGRDPLFLPCTPKGCIELLSRSGISIRGKKAVVMGRSNIVGL 181

Query: 616 PVALLLLKADATVTVVHSRTTXXXXXXXXXXXXXXXXGQANMIKGDWIKPGAAVIDVGTN 795
           PV+LLLLKADATVT+VHSR+                 GQA MIKG WIKPGAAVIDVGTN
Sbjct: 182 PVSLLLLKADATVTIVHSRSVDPESVIREADIIIAAAGQAQMIKGSWIKPGAAVIDVGTN 241

Query: 796 AVDDP 810
           AVDDP
Sbjct: 242 AVDDP 246



 Score = 55.1 bits (131), Expect(2) = e-108
 Identities = 28/52 (53%), Positives = 32/52 (61%)
 Frame = +2

Query: 902  RKSGYRLVGDVDFEQACKXXXXXXXXXXXXXXXXXXXLLKNTLDGAKRKIEQ 1057
            +KSGYRLVGDVDF++ACK                   LL+NTLDGAKR IEQ
Sbjct: 248  KKSGYRLVGDVDFQEACKVAGWITPVPGGVGPMTVAMLLRNTLDGAKRVIEQ 299


>gb|ABK96126.1| unknown [Populus trichocarpa]
          Length = 299

 Score =  367 bits (943), Expect(2) = e-108
 Identities = 186/248 (75%), Positives = 208/248 (83%)
 Frame = +1

Query: 67  MASSEEEQKKATIIDGKAIAHTIRSEIASEVQQLSTKHGKVPGLAVIIVGSRKDSQSYVN 246
           MAS  E   KAT+IDGK IA TIRSEIA EV+QLS K+GKVPGLAV+IVG+RKDSQSYV 
Sbjct: 1   MASQSEH--KATVIDGKLIAQTIRSEIAEEVRQLSEKYGKVPGLAVVIVGNRKDSQSYVG 58

Query: 247 MKRKACAEVGIKSYDINLPEKVPEAELIQKVQELNAHPDIHGILVQLPLPAHINEENVLN 426
           MKRKACAEVGI+S+D++LPE++ EAEL+ KV ELNA+PD+HGILVQLPLP HINEE VL+
Sbjct: 59  MKRKACAEVGIQSFDMDLPEQISEAELVSKVHELNANPDVHGILVQLPLPKHINEEKVLS 118

Query: 427 EISIEKDVDGFHPLNIGKLGMKGRDPLFQPCTPKGCLELLSRSGISVKSKRAVIVGRSNI 606
           EIS+ KDVDGFHPLNIGKL MK R+PLFQPCTPKGCLELL RSG+S+K KRAV+VGRSNI
Sbjct: 119 EISLAKDVDGFHPLNIGKLAMKDREPLFQPCTPKGCLELLHRSGVSIKGKRAVVVGRSNI 178

Query: 607 VGLPVALLLLKADATVTVVHSRTTXXXXXXXXXXXXXXXXGQANMIKGDWIKPGAAVIDV 786
           VGLPV+LLLLKADATVTVVHSR+                 GQ  MIKG WIKPGAAVIDV
Sbjct: 179 VGLPVSLLLLKADATVTVVHSRSNDQESIIREADIIIAAAGQPMMIKGSWIKPGAAVIDV 238

Query: 787 GTNAVDDP 810
           GTNAVDDP
Sbjct: 239 GTNAVDDP 246



 Score = 51.6 bits (122), Expect(2) = e-108
 Identities = 27/52 (51%), Positives = 31/52 (59%)
 Frame = +2

Query: 902  RKSGYRLVGDVDFEQACKXXXXXXXXXXXXXXXXXXXLLKNTLDGAKRKIEQ 1057
            RK+GYRLVGDVDF++ACK                   LLKNTL+GAKR I Q
Sbjct: 248  RKTGYRLVGDVDFKEACKIAGCITPVPGGVGPMTVAMLLKNTLEGAKRVIVQ 299


>gb|AAD01907.1| 5,10-methylenetetrahydrofolate
           dehydrogenase-5,10-methenyltetrahydrofolate
           cyclohydrolase [Pisum sativum]
           gi|6002383|emb|CAB56756.1|
           5,10-methylenetetrahydrofolate dehydrogenase:
           5,10-methenyltetrahydrofolate cyclohydrolase [Pisum
           sativum]
          Length = 294

 Score =  360 bits (924), Expect(2) = e-106
 Identities = 178/238 (74%), Positives = 200/238 (84%)
 Frame = +1

Query: 97  ATIIDGKAIAHTIRSEIASEVQQLSTKHGKVPGLAVIIVGSRKDSQSYVNMKRKACAEVG 276
           AT+IDGKA+A TIRSEIA EV+ LS K+GKVPGLAV+IVG+RKDSQSYV MKRKACAE+G
Sbjct: 2   ATVIDGKAVAQTIRSEIADEVRLLSQKYGKVPGLAVVIVGNRKDSQSYVGMKRKACAELG 61

Query: 277 IKSYDINLPEKVPEAELIQKVQELNAHPDIHGILVQLPLPAHINEENVLNEISIEKDVDG 456
           IKS+DI+LPE   EAE+I+ V ELNA+PD+HGILVQLPLP H+NEE VL EISI KDVDG
Sbjct: 62  IKSFDIDLPEDASEAEIIKNVHELNANPDVHGILVQLPLPKHVNEEKVLTEISISKDVDG 121

Query: 457 FHPLNIGKLGMKGRDPLFQPCTPKGCLELLSRSGISVKSKRAVIVGRSNIVGLPVALLLL 636
           FHPLNIGKL MKGRDPLF PCTPK CLELLSRSG+S+K K+AV+VGRSNIVGLP +LLLL
Sbjct: 122 FHPLNIGKLAMKGRDPLFLPCTPKACLELLSRSGVSIKGKKAVVVGRSNIVGLPASLLLL 181

Query: 637 KADATVTVVHSRTTXXXXXXXXXXXXXXXXGQANMIKGDWIKPGAAVIDVGTNAVDDP 810
           KADATVT+VHS T+                GQA MIKG WIKPGAAVIDVGTN+VDDP
Sbjct: 182 KADATVTIVHSHTSQPETIIREADIVIAAAGQAKMIKGSWIKPGAAVIDVGTNSVDDP 239



 Score = 53.5 bits (127), Expect(2) = e-106
 Identities = 29/52 (55%), Positives = 31/52 (59%)
 Frame = +2

Query: 902  RKSGYRLVGDVDFEQACKXXXXXXXXXXXXXXXXXXXLLKNTLDGAKRKIEQ 1057
            RKSGYRLVGDVDFE+A K                   LLKNTL+GAKR IEQ
Sbjct: 241  RKSGYRLVGDVDFEEASKVAGWITPVPGGVGPMTVTMLLKNTLEGAKRTIEQ 292