BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00000209
         (1276 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      627   e-177
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   597   e-168
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       570   e-160
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   468   e-129
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   464   e-128

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  627 bits (1617), Expect = e-177
 Identities = 299/358 (83%), Positives = 330/358 (92%)
 Frame = +2

Query: 89   MALENKQPKKAAVMELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKF 268
            MA+E KQ KKAA++ELLK+LELGLVP D++KQL+R ELARRLQWGYKPT E+Q+AE+   
Sbjct: 1    MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNL 60

Query: 269  AHSLRHMNIAMEVHTLDSQMYEVPIEFLKIMFGSTLKGSCCYFKDESTTLDEAEIAMLDL 448
             HSLR M IA EV TLDSQ+YE+PIEFLKIM GS LKGSCCYFK++STTLDEAEIAMLDL
Sbjct: 61   THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 120

Query: 449  YCERAQIKDGQTVLDLGCGQGALTLHVAQKYKNCHVTAITNSVSQKEYIEEESRRCNLSN 628
            YCERAQI+DGQ+VLDLGCGQGALTLHVAQKYKNC VTA+TNSVSQKEYIEEESRR NL N
Sbjct: 121  YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 180

Query: 629  VEVICADITTHEMANTYDRILVIELFEHMKNYELLLRKISEWMTPDGLLFIEHVCHKTFA 808
            VEV  ADITTHEMA TYDRILVIELFEHMKNYELLLRKISEW++ DGLLF+EH+CHKTFA
Sbjct: 181  VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 240

Query: 809  YHYEPLDEDDWFTEYVFPAGTVIIPSASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKR 988
            YHYEPLD+DDWFTEYVFPAGT+IIPSASF LYFQDDVSVVNHWTLSGKH+SRTNEEWLKR
Sbjct: 241  YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR 300

Query: 989  LDAHIDAIKPIFETLTGSAEEAVKLINYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 1162
            LDA++D IKP+FETL G+ EEAVKLINYWRGFCLSG+E+ GYNNGEEWM SHVLFK+K
Sbjct: 301  LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  597 bits (1538), Expect = e-168
 Identities = 285/361 (78%), Positives = 325/361 (90%), Gaps = 3/361 (0%)
 Frame = +2

Query: 89   MALENKQ--PKKAAVMELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVL 262
            MA+E KQ  PKKA ++ELLKKLELGLVP DE+K+L+RI+L RRLQWG K T E+Q+A+++
Sbjct: 1    MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60

Query: 263  KFAHSLRHMNIAMEVHTLDSQMYEVPIEFLKIMFGSTLKGSCCYFKDESTTLDEAEIAML 442
               HSLR M IA EV TLD QMYEVPI+FLKIM GS LKGSCCYFK++STTLDEAEIAML
Sbjct: 61   NLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAML 120

Query: 443  DLYCERAQIKDGQTVLDLGCGQGALTLHVAQKYKNCHVTAITNSVSQKEYIEEESRRCNL 622
            +LYCERAQIKDG +VLDLGCGQGALTL+VAQKYKN  VTA+TNSVSQKE+IEEESR+ NL
Sbjct: 121  ELYCERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNL 180

Query: 623  SNVEVICADITTHEMANTYDRILVIELFEHMKNYELLLRKISEWMTPDGLLFIEHVCHKT 802
            SNVEV+ ADITTH+M +TYDRILV+ELFEHMKNYELLLRKI EWM  DGLLF+EH+CHKT
Sbjct: 181  SNVEVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKT 240

Query: 803  FAYHYEPLDEDDWFTEYVFPAGTVIIPSASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWL 982
            FAYHYEP+DEDDWFTEYVFPAGT+IIPSASF LYFQDDVSVVNHWTLSGKH+SRTNEEWL
Sbjct: 241  FAYHYEPIDEDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL 300

Query: 983  KRLDAHIDAIKPIFETLTGSA-EEAVKLINYWRGFCLSGIELCGYNNGEEWMESHVLFKR 1159
            KRLDA+++ IKP+F T+TG   +EA+KLINYWRGFCLSG+E+ GYNNGEEWM SHVLFK+
Sbjct: 301  KRLDANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 360

Query: 1160 K 1162
            K
Sbjct: 361  K 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  570 bits (1469), Expect = e-160
 Identities = 270/344 (78%), Positives = 304/344 (88%)
 Frame = +2

Query: 131  ELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKFAHSLRHMNIAMEVH 310
            EL KKLELGL+P DEVK+LMRI+LARRLQWGYK T E+Q+A+VL   HSLRHMNIAMEV 
Sbjct: 8    ELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVD 67

Query: 311  TLDSQMYEVPIEFLKIMFGSTLKGSCCYFKDESTTLDEAEIAMLDLYCERAQIKDGQTVL 490
            TLDS MYEVP++FLKIM GS LK S CYFKD+STTLDEAEIAM+DL+CERAQIKDG +VL
Sbjct: 68   TLDSNMYEVPLDFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVL 127

Query: 491  DLGCGQGALTLHVAQKYKNCHVTAITNSVSQKEYIEEESRRCNLSNVEVICADITTHEMA 670
            DLGCG G+LT +VAQKYK  HVTA+TNSVSQKEYIEEE+RR NLSNVEV+ ADI THEMA
Sbjct: 128  DLGCGHGSLTFYVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMA 187

Query: 671  NTYDRILVIELFEHMKNYELLLRKISEWMTPDGLLFIEHVCHKTFAYHYEPLDEDDWFTE 850
            +TYDRILV+ LFEHMKNY LLL+KISEWM  DGLLF++HVCHKTFA+H EP+DEDDW +E
Sbjct: 188  DTYDRILVVGLFEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSE 247

Query: 851  YVFPAGTVIIPSASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDAHIDAIKPIFET 1030
            Y FPAGT+IIPSASF LYFQDD SVVNHWTLSGKH+SRT EEWLKRLDA++  I P+F T
Sbjct: 248  YAFPAGTMIIPSASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFAT 307

Query: 1031 LTGSAEEAVKLINYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 1162
            +TGS EEAVKL+NYWRG C  G+EL GYNNGEEWMESHVLFK+K
Sbjct: 308  ITGSEEEAVKLLNYWRGLCFFGVELYGYNNGEEWMESHVLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  468 bits (1205), Expect = e-129
 Identities = 218/356 (61%), Positives = 283/356 (79%)
 Frame = +2

Query: 95   LENKQPKKAAVMELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKFAH 274
            +E KQ KK AV  L+K++E G V  +E++ +M+I++ +RL+WGYKPT+EQQ+A+++ FA 
Sbjct: 1    METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 275  SLRHMNIAMEVHTLDSQMYEVPIEFLKIMFGSTLKGSCCYFKDESTTLDEAEIAMLDLYC 454
            SL+ M +A EV TLD+++YE+P+ FL IM G TLK S  YFKDESTTLDE+E+ M+DLYC
Sbjct: 61   SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYC 120

Query: 455  ERAQIKDGQTVLDLGCGQGALTLHVAQKYKNCHVTAITNSVSQKEYIEEESRRCNLSNVE 634
            ERAQIKDGQ++LDLGCG G+LTLHVAQKY+ C VT ITNSVSQKE+I ++ ++ +LSNVE
Sbjct: 121  ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVE 180

Query: 635  VICADITTHEMANTYDRILVIELFEHMKNYELLLRKISEWMTPDGLLFIEHVCHKTFAYH 814
            +I  D+T  E   TYDRI  + L EHMKNYEL L+K+S W+   GLLF+EH CHK FAY 
Sbjct: 181  IILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQ 240

Query: 815  YEPLDEDDWFTEYVFPAGTVIIPSASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLD 994
            YEPLDEDDW+TEY+FP+GT+++ S+S LLYFQ+DVSVVNHWTLSGKH S   ++WLKRLD
Sbjct: 241  YEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLD 300

Query: 995  AHIDAIKPIFETLTGSAEEAVKLINYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 1162
             +ID +K IFE+  GS E+A+K I YWR FC++  ++   NNGEEWM S VLFK+K
Sbjct: 301  DNIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  464 bits (1193), Expect = e-128
 Identities = 213/344 (61%), Positives = 273/344 (79%)
 Frame = +2

Query: 131  ELLKKLELGLVPCDEVKQLMRIELARRLQWGYKPTNEQQVAEVLKFAHSLRHMNIAMEVH 310
            ELL+ +ELGL+P  E++QL+R+EL +RLQWGYK T+E+Q++++L   HSL+ M +A E+ 
Sbjct: 8    ELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEME 67

Query: 311  TLDSQMYEVPIEFLKIMFGSTLKGSCCYFKDESTTLDEAEIAMLDLYCERAQIKDGQTVL 490
             LD ++YE P+EFLKI  GS +K S  Y+ DESTTLDEAEIAMLDLY ERAQIKDGQ+VL
Sbjct: 68   NLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVL 127

Query: 491  DLGCGQGALTLHVAQKYKNCHVTAITNSVSQKEYIEEESRRCNLSNVEVICADITTHEMA 670
            DLGCG GA+ L  A K+K C  T +T+SV QK+YIE + +   L+NV+V+ ADITT+E  
Sbjct: 128  DLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETE 187

Query: 671  NTYDRILVIELFEHMKNYELLLRKISEWMTPDGLLFIEHVCHKTFAYHYEPLDEDDWFTE 850
              +DRI  +EL EHMKNY+LLL+KISEWM  DGLLF+EHVCHKT AYHYEP+D +DW+T 
Sbjct: 188  ERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTN 247

Query: 851  YVFPAGTVIIPSASFLLYFQDDVSVVNHWTLSGKHYSRTNEEWLKRLDAHIDAIKPIFET 1030
            Y+FPAGT+ + SAS LLYFQDDVSVVN WTLSGKHYSR++EEWLK +D +I   K I  +
Sbjct: 248  YIFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRS 307

Query: 1031 LTGSAEEAVKLINYWRGFCLSGIELCGYNNGEEWMESHVLFKRK 1162
            +T + +EA+KL+N+WR FC+ G EL GY NGEEWM +H+LFK+K
Sbjct: 308  ITKTEKEAIKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351