BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00035765
         (487 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel prot...    91   1e-16
ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel prot...    91   1e-16
ref|XP_003592677.1| mscS family protein, putative [Medicago trun...    84   2e-14
ref|NP_177982.1| mechanosensitive channel of small conductance-l...    81   1e-13
ref|XP_002889201.1| mechanosensitive ion channel domain-containi...    78   8e-13

>ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score = 90.9 bits (224), Expect = 1e-16
 Identities = 68/179 (37%), Positives = 87/179 (48%), Gaps = 28/179 (15%)
 Frame = -3

Query: 467 NSGSTIWRGSSYDFWSDHXXXXXXXXNVVAGNSSNEDGFDFQN--KNVEDPPSKLIGKFL 294
           N   TIWR SSYDFW+D             G +   D F+F+   K+VEDPPSKLIG+FL
Sbjct: 73  NGSGTIWRESSYDFWNDSDNRRNYGE----GGAKTTDDFEFRQHRKDVEDPPSKLIGQFL 128

Query: 293 HRQKASGEIAXXXXXXXXXLSEAVVGNSGLQAIQEVPF--SPKEAKVSFR---EQGENWS 129
           H+QKASGE++           E     + L  + E P   S +E KVSF    E  EN S
Sbjct: 129 HKQKASGEMSLDMDMEML---ELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185

Query: 128 ----------------PSPEAVHRRSNGSVPEEGRGGEVLRCTSNNA-----SFKRNSS 15
                             P    RR++GS  E+    EVLRC+SN++     SF+R SS
Sbjct: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSSFQRDVSFQRKSS 244


>ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
           sativus]
          Length = 923

 Score = 90.5 bits (223), Expect = 1e-16
 Identities = 68/179 (37%), Positives = 86/179 (48%), Gaps = 28/179 (15%)
 Frame = -3

Query: 467 NSGSTIWRGSSYDFWSDHXXXXXXXXNVVAGNSSNEDGFDFQN--KNVEDPPSKLIGKFL 294
           N   TIWR SSYDFW+D             G +   D F+F+   K+VEDPPSKLIG+FL
Sbjct: 73  NGSGTIWRESSYDFWNDSDNRRNYGE----GGAKTTDDFEFRQHRKDVEDPPSKLIGQFL 128

Query: 293 HRQKASGEIAXXXXXXXXXLSEAVVGNSGLQAIQEVPF--SPKEAKVSFR---EQGENWS 129
           H+QKASGE++           E     + L  + E P   S +E KVSF    E  EN S
Sbjct: 129 HKQKASGEMSLDMDMEML---ELPQDKTPLSTVAESPMRRSSRELKVSFESISEISENDS 185

Query: 128 ----------------PSPEAVHRRSNGSVPEEGRGGEVLRCTSN-----NASFKRNSS 15
                             P    RR++GS  E+    EVLRC+SN     + SF+R SS
Sbjct: 186 MRRRHRDSPLDEEHRGQQPRQCDRRAHGSNGEDDGAAEVLRCSSNSFFQRDVSFQRKSS 244


>ref|XP_003592677.1| mscS family protein, putative [Medicago truncatula]
           gi|355481725|gb|AES62928.1| mscS family protein,
           putative [Medicago truncatula]
          Length = 926

 Score = 83.6 bits (205), Expect = 2e-14
 Identities = 66/183 (36%), Positives = 86/183 (46%), Gaps = 31/183 (16%)
 Frame = -3

Query: 470 NNSGSTIWRGSSYDFWSDHXXXXXXXXNVVAGNSSNEDGFDFQNK-NVEDPPSKLIGKFL 294
           + + + IWR SSYDFW+D             G++  ++ FDF+NK  +EDPPS+LIGKFL
Sbjct: 62  SQANNKIWRESSYDFWND------------TGDNVRDESFDFRNKAKLEDPPSQLIGKFL 109

Query: 293 HRQKASGEIAXXXXXXXXXLSEAVVGNSG-LQAIQEVP-FSPKEAKVSFREQGENW---- 132
           H+Q+ASG++          L     G  G L  ++E P    +E KVSF E   N     
Sbjct: 110 HKQRASGDMLLDMDLEMEELQNEGNGADGKLTPVEESPTVIQRELKVSFEEPASNSNGID 169

Query: 131 --------------SPS----------PEAVHRRSNGSVPEEGRGGEVLRCTSNNASFKR 24
                         SPS          P    RRS       G   EVLRC S+NASF+R
Sbjct: 170 AVVNDPVRRRHSKDSPSMGEYARPPQPPNHDRRRSPSPAGHGGGDCEVLRC-SSNASFER 228

Query: 23  NSS 15
           N S
Sbjct: 229 NLS 231


>ref|NP_177982.1| mechanosensitive channel of small conductance-like 6 [Arabidopsis
           thaliana] gi|75213461|sp|Q9SYM1.1|MSL6_ARATH RecName:
           Full=Mechanosensitive ion channel protein 6; AltName:
           Full=Mechanosensitive channel of small conductance-like
           6; AltName: Full=MscS-Like protein 6
           gi|4836872|gb|AAD30575.1|AC007260_6 Hypothetical protein
           [Arabidopsis thaliana] gi|332198006|gb|AEE36127.1|
           mechanosensitive channel of small conductance-like 6
           [Arabidopsis thaliana]
          Length = 856

 Score = 80.9 bits (198), Expect = 1e-13
 Identities = 58/171 (33%), Positives = 80/171 (46%), Gaps = 13/171 (7%)
 Frame = -3

Query: 479 NNNNNSGST---IWRGSSYDFWSDHXXXXXXXXNVVA----------GNSSNEDGFDFQN 339
           NNN  SG T   IWR  SYDFW+D         N  A          G    ++GF+F+ 
Sbjct: 21  NNNGVSGETVGKIWRDGSYDFWTDGEGNLNKGHNAAAVDSDRSAATTGEQQKDEGFEFRR 80

Query: 338 KNVEDPPSKLIGKFLHRQKASGEIAXXXXXXXXXLSEAVVGNSGLQAIQEVPFSPKEAKV 159
              EDPP+KLIG+FLH+Q+ASGEI            +  +G   LQ+    P S      
Sbjct: 81  G--EDPPTKLIGQFLHKQQASGEICL----------DMDLGMDELQSRGLTPVS------ 122

Query: 158 SFREQGENWSPSPEAVHRRSNGSVPEEGRGGEVLRCTSNNASFKRNSSSFM 6
               +    S   + V RR + S       GEV++C+ NNA  +R+SS+ +
Sbjct: 123 ----ESPRVSTKRDPVGRRDSRSNTNNNDDGEVVKCSGNNAPIQRSSSTLL 169


>ref|XP_002889201.1| mechanosensitive ion channel domain-containing protein [Arabidopsis
           lyrata subsp. lyrata] gi|297335042|gb|EFH65460.1|
           mechanosensitive ion channel domain-containing protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 857

 Score = 77.8 bits (190), Expect = 8e-13
 Identities = 61/172 (35%), Positives = 85/172 (49%), Gaps = 14/172 (8%)
 Frame = -3

Query: 479 NNNNNSGST---IWRGSSYDFWSDHXXXXXXXXNVVAGNSSN---------EDGFDFQNK 336
           +NN  SG T   IWR  SYDFW+D         N  A +S           ++GF+F+  
Sbjct: 18  DNNGVSGETVGRIWRDGSYDFWTDGEGNLNKGHNAAAVDSDRSAATTEEQQDEGFEFRRG 77

Query: 335 NVEDPPSKLIGKFLHRQKASGEIAXXXXXXXXXLSEAVVGNSGLQA--IQEVPFSPKEAK 162
             EDPP+KLIG+FLH+Q+ASGEI            +  +G   LQ+  +  V  SP  AK
Sbjct: 78  --EDPPTKLIGQFLHKQQASGEICL----------DMDLGMDELQSRGLTPVSESPTSAK 125

Query: 161 VSFREQGENWSPSPEAVHRRSNGSVPEEGRGGEVLRCTSNNASFKRNSSSFM 6
           V         +  P+ V RR + S       GEV++C S NA  +R+SS+ +
Sbjct: 126 VP--------TKPPDPVGRRDSRSNNNNNDDGEVVKC-SGNAPIQRSSSNLL 168