BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00029087
         (1124 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   358   2e-96
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              358   2e-96
ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa] g...   355   1e-95
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]                  354   2e-95
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...   353   4e-95

>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
            vinifera]
          Length = 793

 Score =  358 bits (918), Expect = 2e-96
 Identities = 173/273 (63%), Positives = 213/273 (78%)
 Frame = +2

Query: 2    ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 181
            AT+F PE SDAR+SWAK  VLTTVVDDF+D+GGS+EEL NLI L+EKWD   A D CSEQ
Sbjct: 517  ATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQ 576

Query: 182  VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 361
            VEIV++AL STI E+G KA  WQ R+VT HI++IWL L++SM++E++W  NKS PTMDEY
Sbjct: 577  VEIVFSALHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEY 636

Query: 362  NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 541
              NAY SFALGPIVLPALYFVGP+LSEE V   ECH L++LMSTCGR LNDI  FKRE+ 
Sbjct: 637  MTNAYVSFALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLMSTCGRLLNDIHSFKRESK 696

Query: 542  EGKLNGVSLLMIHDPTVSNEEEAMKKMRNTIDSCRRELMKLLLQTNGSIVPRPCKDLFWK 721
            EGK N ++L MIH   V+ EE+A+++M+  + S RREL +L+LQ  GS VPR CKDLFWK
Sbjct: 697  EGKANALALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLVLQEKGSTVPRICKDLFWK 756

Query: 722  MCRVVHLFYMSNDGFTSGEQMVSDVKAVMYVPL 820
            M +V+H FY  +DGFTS   M+  VK+V+Y P+
Sbjct: 757  MSKVLHTFYEKDDGFTS-HDMLRAVKSVIYEPV 788


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  358 bits (918), Expect = 2e-96
 Identities = 173/273 (63%), Positives = 213/273 (78%)
 Frame = +2

Query: 2    ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 181
            AT+F PE SDAR+SWAK  VLTTVVDDF+D+GGS+EEL NLI L+EKWD   A D CSEQ
Sbjct: 550  ATIFSPEQSDARLSWAKNSVLTTVVDDFFDIGGSEEELLNLIQLVEKWDIDVAVDCCSEQ 609

Query: 182  VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 361
            VEIV++AL STI E+G KA  WQ R+VT HI++IWL L++SM++E++W  NKS PTMDEY
Sbjct: 610  VEIVFSALHSTISEIGVKASAWQARNVTSHIIDIWLKLLRSMLQEAQWVSNKSAPTMDEY 669

Query: 362  NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 541
              NAY SFALGPIVLPALYFVGP+LSEE V   ECH L++LMSTCGR LNDI  FKRE+ 
Sbjct: 670  MTNAYVSFALGPIVLPALYFVGPKLSEEVVEGPECHKLYKLMSTCGRLLNDIHSFKRESK 729

Query: 542  EGKLNGVSLLMIHDPTVSNEEEAMKKMRNTIDSCRRELMKLLLQTNGSIVPRPCKDLFWK 721
            EGK N ++L MIH   V+ EE+A+++M+  + S RREL +L+LQ  GS VPR CKDLFWK
Sbjct: 730  EGKANALALHMIHGNGVTTEEQAIREMKGLVKSQRRELQRLVLQEKGSTVPRICKDLFWK 789

Query: 722  MCRVVHLFYMSNDGFTSGEQMVSDVKAVMYVPL 820
            M +V+H FY  +DGFTS   M+  VK+V+Y P+
Sbjct: 790  MSKVLHTFYEKDDGFTS-HDMLRAVKSVIYEPV 821


>ref|XP_002311286.1| ent-kaurene synthase [Populus trichocarpa]
            gi|222851106|gb|EEE88653.1| ent-kaurene synthase [Populus
            trichocarpa]
          Length = 743

 Score =  355 bits (910), Expect = 1e-95
 Identities = 169/277 (61%), Positives = 217/277 (78%)
 Frame = +2

Query: 2    ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 181
            ATLF PELSDARMSWAK GVLTTVVDDF+DVGGS+EEL NLI+L+E+WD   +ADFCSE+
Sbjct: 467  ATLFAPELSDARMSWAKNGVLTTVVDDFFDVGGSEEELVNLIELIERWDVNGSADFCSEE 526

Query: 182  VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 361
            VEI+Y+A+ STI E+G+K+F WQ RDV   +++IWL L+KSM+ E++W+ NKSVPT+DEY
Sbjct: 527  VEIIYSAIHSTISEIGDKSFGWQGRDVKSQVIKIWLDLLKSMLTEAQWSSNKSVPTLDEY 586

Query: 362  NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 541
               A+ SFALGPIVLPALYFVGP+LSEE     E  NL+++ STCGR LND + FKRE+ 
Sbjct: 587  MTTAHVSFALGPIVLPALYFVGPKLSEEVAGHPELLNLYKVTSTCGRLLNDWRSFKRESE 646

Query: 542  EGKLNGVSLLMIHDPTVSNEEEAMKKMRNTIDSCRRELMKLLLQTNGSIVPRPCKDLFWK 721
            EGKLN +SL MIH    S EEE ++  +  IDS RR+L++L+LQ   SI+PRPCKDLFW 
Sbjct: 647  EGKLNAISLYMIHSGGASTEEETIEHFKGLIDSQRRQLLQLVLQEKDSIIPRPCKDLFWN 706

Query: 722  MCRVVHLFYMSNDGFTSGEQMVSDVKAVMYVPLTFPQ 832
            M +++H FYM +DGFTS E M + VKA++  P++  +
Sbjct: 707  MIKLLHTFYMKDDGFTSNE-MRNVVKAIINEPISLDE 742


>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
          Length = 737

 Score =  354 bits (909), Expect = 2e-95
 Identities = 181/273 (66%), Positives = 214/273 (78%)
 Frame = +2

Query: 2    ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 181
            ATLF PELSDAR+SWAK GVLTTVVDDF+DVGGS+EEL NLI L+EKWD     D CSE 
Sbjct: 462  ATLFPPELSDARISWAKNGVLTTVVDDFFDVGGSEEELINLIQLVEKWDVNVGVDCCSEN 521

Query: 182  VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 361
            VEI+++AL++TI E+G +AF  Q R VT H++EIWL LIKSM KE+EW  NKSVPTM+EY
Sbjct: 522  VEIIFSALKNTINEIGARAFTRQGRSVTSHVIEIWLDLIKSMFKEAEWLRNKSVPTMEEY 581

Query: 362  NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 541
              NAY SFALGPIVLPALY VGP+LSEEAV S E  +L+RLMST GR LNDIQGFKRE+ 
Sbjct: 582  MENAYVSFALGPIVLPALYLVGPKLSEEAVGSSEFDHLYRLMSTAGRLLNDIQGFKRESA 641

Query: 542  EGKLNGVSLLMIHDPTVSNEEEAMKKMRNTIDSCRRELMKLLLQTNGSIVPRPCKDLFWK 721
            EGKLN VSL MIH   V+ EEEA+ +M+N I S RREL++L+L   GS+VPR CKDLFW 
Sbjct: 642  EGKLNAVSLAMIHGNGVT-EEEAINEMKNVIVSKRRELLRLVLLEKGSVVPRACKDLFWN 700

Query: 722  MCRVVHLFYMSNDGFTSGEQMVSDVKAVMYVPL 820
            M +V+HLFY  +DGFT+   M+  V AVM  P+
Sbjct: 701  MSKVLHLFYAKHDGFTA-HDMMKTVNAVMEEPI 732


>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 808

 Score =  353 bits (906), Expect = 4e-95
 Identities = 172/275 (62%), Positives = 216/275 (78%)
 Frame = +2

Query: 2    ATLFHPELSDARMSWAKGGVLTTVVDDFYDVGGSKEELANLIDLLEKWDEVSAADFCSEQ 181
            ATLF PELSDAR+SWAK GVLTTVVDDF+DVGGS EEL NLI L+EKWD   +  FCSEQ
Sbjct: 462  ATLFAPELSDARLSWAKNGVLTTVVDDFFDVGGSVEELINLIQLIEKWDVDESTHFCSEQ 521

Query: 182  VEIVYNALRSTIVELGEKAFRWQQRDVTRHIVEIWLTLIKSMMKESEWTENKSVPTMDEY 361
            VEI+++ALRSTI E+G+KAF WQ R VT H+++IWL L+KSM+ E+ WT++KS+PT+DEY
Sbjct: 522  VEIIFSALRSTISEIGDKAFTWQGRKVTSHVIKIWLDLLKSMLTETLWTKSKSIPTLDEY 581

Query: 362  NNNAYTSFALGPIVLPALYFVGPRLSEEAVRSEECHNLFRLMSTCGRNLNDIQGFKRENN 541
              N Y SFALGPIVLPAL+ VGP+L+EE VR  E H+LF+ M TCGR LND +GF+RE+ 
Sbjct: 582  MINGYVSFALGPIVLPALFLVGPKLTEEDVRDPELHDLFKAMGTCGRLLNDWRGFQRESK 641

Query: 542  EGKLNGVSLLMIHDPTVSNEEEAMKKMRNTIDSCRRELMKLLLQTNGSIVPRPCKDLFWK 721
            EGKLN VSL MI      NEEEA++K++  I+S R EL++L+L+   S +PR CKDLFWK
Sbjct: 642  EGKLNAVSLHMIQGNGGVNEEEAIRKIKGLINSQRSELLRLVLREKNSNIPRACKDLFWK 701

Query: 722  MCRVVHLFYMSNDGFTSGEQMVSDVKAVMYVPLTF 826
            M +V+HLFY+ +DGFTS E M+S   AV+  P+ F
Sbjct: 702  MIKVLHLFYLKDDGFTSNE-MISTANAVITEPVAF 735