BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00024090
         (738 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002268807.2| PREDICTED: pentatricopeptide repeat-containi...   404   e-110
emb|CAN74731.1| hypothetical protein VITISV_037837 [Vitis vinifera]   404   e-110
ref|XP_003532067.1| PREDICTED: pentatricopeptide repeat-containi...   391   e-107
ref|XP_003551954.1| PREDICTED: pentatricopeptide repeat-containi...   386   e-105
ref|XP_004156032.1| PREDICTED: LOW QUALITY PROTEIN: pentatricope...   379   e-103

>ref|XP_002268807.2| PREDICTED: pentatricopeptide repeat-containing protein At3g49710-like
            [Vitis vinifera]
          Length = 719

 Score =  404 bits (1037), Expect = e-110
 Identities = 195/245 (79%), Positives = 216/245 (88%)
 Frame = +2

Query: 2    SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQT 181
            SFVCVISACSNLSSPSQGKQ+HSLALKSDIP NRISV NALIAMYSKCGNLQDARRLF  
Sbjct: 345  SFVCVISACSNLSSPSQGKQIHSLALKSDIPSNRISVDNALIAMYSKCGNLQDARRLFDR 404

Query: 182  MPEHNTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEG 361
            M EHNTVS NS+IAGYAQHG   +SL +FQ MLE ++ PTSITF+SVLS+CAHTG V+EG
Sbjct: 405  MAEHNTVSLNSMIAGYAQHGIEMESLHLFQWMLERQIAPTSITFISVLSACAHTGRVEEG 464

Query: 362  WKYFNMMKEKFGIEPDAEHYSCMIDLLGRAGKLDEAERLIETMPFDPCSISWGALLGACR 541
            W YFNMMKEKF IEP+AEHYSCMIDLLGRAGKL EAE LI  MPF+P SI W +LLGACR
Sbjct: 465  WNYFNMMKEKFNIEPEAEHYSCMIDLLGRAGKLSEAENLIARMPFNPGSIGWASLLGACR 524

Query: 542  THGNVELGAKAASHCLRLEPNNAAPYVMLANIYASAGRWEEYATVKRLMRDRGVKKKPGC 721
            THGN+EL  KAA+  L+LEP+NAAPYV+L+N+YASAGRWEE ATV++ MRDRGVKKKPGC
Sbjct: 525  THGNIELAVKAANQVLQLEPSNAAPYVVLSNMYASAGRWEEVATVRKFMRDRGVKKKPGC 584

Query: 722  SWIEV 736
            SWIEV
Sbjct: 585  SWIEV 589



 Score = 71.6 bits (174), Expect = 2e-10
 Identities = 53/160 (33%), Positives = 87/160 (54%), Gaps = 3/160 (1%)
 Frame = +2

Query: 59  QLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQTMPE-HNTVSFNSIIAGYAQ 235
           QLHS+A+ S    + +SV+NAL+  Y K G+L DA+R+F  M    + VS+NS+I  Y Q
Sbjct: 159 QLHSVAVSSGFD-SYVSVNNALLTYYGKNGDLDDAKRVFYGMGGIRDEVSWNSMIVAYGQ 217

Query: 236 HGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVD-EGWKYFNMMKEKFGIEPDA 412
           H  G+K+L +FQ+M+   +     T  SVL+  A T + D  G   F+    K G   ++
Sbjct: 218 HQEGSKALGLFQEMVRRGLNVDMFTLASVLT--AFTCLEDLSGGLQFHGQLIKTGFHQNS 275

Query: 413 EHYSCMIDLLGR-AGKLDEAERLIETMPFDPCSISWGALL 529
              S +IDL  +  G + +  ++ E +  +P  + W  ++
Sbjct: 276 HVGSGLIDLYSKCGGGMSDCRKVFEEIT-EPDLVLWNTMV 314



 Score = 70.1 bits (170), Expect = 5e-10
 Identities = 61/220 (27%), Positives = 98/220 (44%), Gaps = 33/220 (15%)
 Frame = +2

Query: 14  VISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQTMPEH 193
           ++  C      S GK LHSL +KS IP +    SN  I +YSKCG L  AR+ FQ + + 
Sbjct: 14  LLKTCIAERDLSTGKSLHSLYIKSFIPPSTY-FSNHFILLYSKCGRLAWARKAFQDISDP 72

Query: 194 NTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEGWKYF 373
           N  SFN+IIA YA+      +  +F ++ E    P  +++ +++S+ A  G        F
Sbjct: 73  NVFSFNAIIAAYAKESRPLIAHQLFDQIPE----PDLVSYNTLISAYADCGETAPALGLF 128

Query: 374 NMMKEKFGIEPDAEHYSCMI---------------------------------DLLGRAG 454
           + M+E  G++ D    S +I                                    G+ G
Sbjct: 129 SGMRE-MGLDMDGFTLSAVITACCDDVGLIGQLHSVAVSSGFDSYVSVNNALLTYYGKNG 187

Query: 455 KLDEAERLIETMPFDPCSISWGALLGACRTHGNVELGAKA 574
            LD+A+R+   M      +SW +++ A   +G  + G+KA
Sbjct: 188 DLDDAKRVFYGMGGIRDEVSWNSMIVA---YGQHQEGSKA 224


>emb|CAN74731.1| hypothetical protein VITISV_037837 [Vitis vinifera]
          Length = 719

 Score =  404 bits (1037), Expect = e-110
 Identities = 195/245 (79%), Positives = 216/245 (88%)
 Frame = +2

Query: 2    SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQT 181
            SFVCVISACSNLSSPSQGKQ+HSLALKSDIP NRISV NALIAMYSKCGNLQDARRLF  
Sbjct: 345  SFVCVISACSNLSSPSQGKQIHSLALKSDIPSNRISVDNALIAMYSKCGNLQDARRLFDR 404

Query: 182  MPEHNTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEG 361
            M EHNTVS NS+IAGYAQHG   +SL +FQ MLE ++ PTSITF+SVLS+CAHTG V+EG
Sbjct: 405  MAEHNTVSLNSMIAGYAQHGIEMESLHLFQWMLERQIAPTSITFISVLSACAHTGRVEEG 464

Query: 362  WKYFNMMKEKFGIEPDAEHYSCMIDLLGRAGKLDEAERLIETMPFDPCSISWGALLGACR 541
            W YFNMMKEKF IEP+AEHYSCMIDLLGRAGKL EAE LI  MPF+P SI W +LLGACR
Sbjct: 465  WNYFNMMKEKFNIEPEAEHYSCMIDLLGRAGKLSEAENLIARMPFNPGSIGWASLLGACR 524

Query: 542  THGNVELGAKAASHCLRLEPNNAAPYVMLANIYASAGRWEEYATVKRLMRDRGVKKKPGC 721
            THGN+EL  KAA+  L+LEP+NAAPYV+L+N+YASAGRWEE ATV++ MRDRGVKKKPGC
Sbjct: 525  THGNIELAVKAANQVLQLEPSNAAPYVVLSNMYASAGRWEEVATVRKFMRDRGVKKKPGC 584

Query: 722  SWIEV 736
            SWIEV
Sbjct: 585  SWIEV 589



 Score = 71.6 bits (174), Expect = 2e-10
 Identities = 53/160 (33%), Positives = 87/160 (54%), Gaps = 3/160 (1%)
 Frame = +2

Query: 59  QLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQTMPE-HNTVSFNSIIAGYAQ 235
           QLHS+A+ S    + +SV+NAL+  Y K G+L DA+R+F  M    + VS+NS+I  Y Q
Sbjct: 159 QLHSVAVSSGFD-SYVSVNNALLTYYGKNGDLDDAKRVFYGMGGIRDEVSWNSMIVAYGQ 217

Query: 236 HGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVD-EGWKYFNMMKEKFGIEPDA 412
           H  G+K+L +FQ+M+   +     T  SVL+  A T + D  G   F+    K G   ++
Sbjct: 218 HQEGSKALGLFQEMVRRGLNVDMFTLASVLT--AFTCLEDLSGGLQFHGQLIKTGFHQNS 275

Query: 413 EHYSCMIDLLGR-AGKLDEAERLIETMPFDPCSISWGALL 529
              S +IDL  +  G + +  ++ E +  +P  + W  ++
Sbjct: 276 HVGSGLIDLYSKCGGGMSDCRKVFEEIT-EPDLVLWNTMV 314



 Score = 69.7 bits (169), Expect = 6e-10
 Identities = 61/220 (27%), Positives = 98/220 (44%), Gaps = 33/220 (15%)
 Frame = +2

Query: 14  VISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQTMPEH 193
           ++  C      S GK LHSL +KS IP +    SN  I +YSKCG L  AR+ FQ + + 
Sbjct: 14  LLKTCIAERDLSTGKSLHSLYIKSFIPPSTY-FSNHFILLYSKCGRLAWARKAFQDISDP 72

Query: 194 NTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEGWKYF 373
           N  SFN+IIA YA+      +  +F ++ E    P  +++ +++S+ A  G        F
Sbjct: 73  NVFSFNAIIAAYAKESRPLIAHQLFDQIPE----PDLVSYNTLISAYADCGETAPALGLF 128

Query: 374 NMMKEKFGIEPDAEHYSCMI---------------------------------DLLGRAG 454
           + M+E  G++ D    S +I                                    G+ G
Sbjct: 129 SGMRE-MGLDMDXFTLSAVITACCDDVGLIGQLHSVAVSSGFDSYVSVNNALLTYYGKNG 187

Query: 455 KLDEAERLIETMPFDPCSISWGALLGACRTHGNVELGAKA 574
            LD+A+R+   M      +SW +++ A   +G  + G+KA
Sbjct: 188 DLDDAKRVFYGMGGIRDEVSWNSMIVA---YGQHQEGSKA 224


>ref|XP_003532067.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710-like
            [Glycine max]
          Length = 722

 Score =  391 bits (1005), Expect = e-107
 Identities = 186/245 (75%), Positives = 214/245 (87%)
 Frame = +2

Query: 2    SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQT 181
            SFVCV SACSNLSSPS GKQ+H+LA+KSD+P NR+SV+NAL+AMYSKCGN+ DARR+F T
Sbjct: 348  SFVCVTSACSNLSSPSLGKQVHALAIKSDVPYNRVSVNNALVAMYSKCGNVHDARRVFDT 407

Query: 182  MPEHNTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEG 361
            MPEHNTVS NS+IAGYAQHG   +SL +F+ MLE ++ P SITF++VLS+C HTG V+EG
Sbjct: 408  MPEHNTVSLNSMIAGYAQHGVEVESLRLFELMLEKDIAPNSITFIAVLSACVHTGKVEEG 467

Query: 362  WKYFNMMKEKFGIEPDAEHYSCMIDLLGRAGKLDEAERLIETMPFDPCSISWGALLGACR 541
             KYFNMMKE+F IEP+AEHYSCMIDLLGRAGKL EAER+IETMPF+P SI W  LLGACR
Sbjct: 468  QKYFNMMKERFCIEPEAEHYSCMIDLLGRAGKLKEAERIIETMPFNPGSIEWATLLGACR 527

Query: 542  THGNVELGAKAASHCLRLEPNNAAPYVMLANIYASAGRWEEYATVKRLMRDRGVKKKPGC 721
             HGNVEL  KAA+  LRLEP NAAPYVML+N+YASA RWEE ATVKRLMR+RGVKKKPGC
Sbjct: 528  KHGNVELAVKAANEFLRLEPYNAAPYVMLSNMYASAARWEEAATVKRLMRERGVKKKPGC 587

Query: 722  SWIEV 736
            SWIE+
Sbjct: 588  SWIEI 592



 Score = 64.3 bits (155), Expect = 2e-08
 Identities = 57/216 (26%), Positives = 93/216 (43%), Gaps = 34/216 (15%)
 Frame = +2

Query: 2   SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDA------ 163
           +F  ++ AC        GK LH+L  KS IP +   +SN    +YSKCG+L +A      
Sbjct: 11  TFRNLLKACIAQRDLITGKILHALYFKSLIPPSTY-LSNHFTLLYSKCGSLHNAQTSFHL 69

Query: 164 -------------------------RRLFQTMPEHNTVSFNSIIAGYAQHGHGTKSLLMF 268
                                    RR+F  +P+ + VS+N++IA YA  G    +L +F
Sbjct: 70  TQYPNVFSYNTLINAYAKHSLIHIARRVFDEIPQPDIVSYNTLIAAYADRGECGPTLRLF 129

Query: 269 QKMLELEVLPTSITFVSVLSSCA-HTGMVDEGWKYFNMMKEKFGIEPDAEHYSCMIDLLG 445
           +++ EL +     T   V+++C    G+V    +  +      G +  A   + ++    
Sbjct: 130 EEVRELRLGLDGFTLSGVITACGDDVGLV----RQLHCFVVVCGHDCYASVNNAVLACYS 185

Query: 446 RAGKLDEAERLIETM--PFDPCSISWGALLGACRTH 547
           R G L EA R+   M        +SW A++ AC  H
Sbjct: 186 RKGFLSEARRVFREMGEGGGRDEVSWNAMIVACGQH 221



 Score = 60.5 bits (145), Expect = 4e-07
 Identities = 46/146 (31%), Positives = 80/146 (54%), Gaps = 5/146 (3%)
 Frame = +2

Query: 107 SVSNALIAMYSKCGNLQDARRLFQTMPE---HNTVSFNSIIAGYAQHGHGTKSLLMFQKM 277
           SV+NA++A YS+ G L +ARR+F+ M E    + VS+N++I    QH  G +++ +F++M
Sbjct: 175 SVNNAVLACYSRKGFLSEARRVFREMGEGGGRDEVSWNAMIVACGQHREGMEAVGLFREM 234

Query: 278 LELEVLPTSITFVSVLSSCAHTGMVD-EGWKYFNMMKEKFGIEPDAEHYSCMIDLLGR-A 451
           +   +     T  SVL+  A T + D  G + F+ M  K G   ++   S +IDL  + A
Sbjct: 235 VRRGLKVDMFTMASVLT--AFTCVKDLVGGRQFHGMMIKSGFHGNSHVGSGLIDLYSKCA 292

Query: 452 GKLDEAERLIETMPFDPCSISWGALL 529
           G + E  ++ E +   P  + W  ++
Sbjct: 293 GSMVECRKVFEEIT-APDLVLWNTMI 317


>ref|XP_003551954.1| PREDICTED: pentatricopeptide repeat-containing protein At3g49710-like
            [Glycine max]
          Length = 722

 Score =  386 bits (991), Expect = e-105
 Identities = 183/245 (74%), Positives = 213/245 (86%)
 Frame = +2

Query: 2    SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQT 181
            SFVCV SACSNLSSPS GKQ+H+LA+KSDIP NR+SV+NAL+AMYSKCGN+ DARR+F T
Sbjct: 348  SFVCVTSACSNLSSPSVGKQVHALAIKSDIPYNRVSVNNALVAMYSKCGNVHDARRVFDT 407

Query: 182  MPEHNTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEG 361
            MPEHN VS NS+IAGYAQHG   +SL +F+ ML+ ++ P +ITF++VLS+C HTG V+EG
Sbjct: 408  MPEHNMVSLNSMIAGYAQHGVEVESLRLFELMLQKDIAPNTITFIAVLSACVHTGKVEEG 467

Query: 362  WKYFNMMKEKFGIEPDAEHYSCMIDLLGRAGKLDEAERLIETMPFDPCSISWGALLGACR 541
             KYFNMMKE+F IEP+AEHYSCMIDLLGRAGKL EAER+IETMPF+P SI W  LLGACR
Sbjct: 468  QKYFNMMKERFRIEPEAEHYSCMIDLLGRAGKLKEAERIIETMPFNPGSIEWATLLGACR 527

Query: 542  THGNVELGAKAASHCLRLEPNNAAPYVMLANIYASAGRWEEYATVKRLMRDRGVKKKPGC 721
             HGNVEL  KAA+  L+LEP NAAPYVML+N+YASA RWEE ATVKRLMR+RGVKKKPGC
Sbjct: 528  KHGNVELAVKAANEFLQLEPYNAAPYVMLSNMYASAARWEEAATVKRLMRERGVKKKPGC 587

Query: 722  SWIEV 736
            SWIE+
Sbjct: 588  SWIEI 592



 Score = 58.9 bits (141), Expect = 1e-06
 Identities = 46/146 (31%), Positives = 79/146 (54%), Gaps = 5/146 (3%)
 Frame = +2

Query: 107 SVSNALIAMYSKCGNLQDARRLFQTMPE---HNTVSFNSIIAGYAQHGHGTKSLLMFQKM 277
           SV+NA++A YS+ G L +ARR+F+ M E    + VS+N++I    QH  G +++ +F++M
Sbjct: 175 SVNNAVLACYSRKGFLNEARRVFREMGEGGGRDEVSWNAMIVACGQHREGLEAVELFREM 234

Query: 278 LELEVLPTSITFVSVLSSCAHTGMVD-EGWKYFNMMKEKFGIEPDAEHYSCMIDLLGR-A 451
           +   +     T  SVL+  A T + D  G   F+ M  K G   ++   S +IDL  + A
Sbjct: 235 VRRGLKVDMFTMASVLT--AFTCVKDLVGGMQFHGMMIKSGFHGNSHVGSGLIDLYSKCA 292

Query: 452 GKLDEAERLIETMPFDPCSISWGALL 529
           G + E  ++ E +   P  + W  ++
Sbjct: 293 GGMVECRKVFEEIA-APDLVLWNTMI 317


>ref|XP_004156032.1| PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing
            protein At3g49710-like [Cucumis sativus]
          Length = 720

 Score =  379 bits (973), Expect = e-103
 Identities = 182/245 (74%), Positives = 214/245 (87%)
 Frame = +2

Query: 2    SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQT 181
            S VCVISACSN+SSPSQG+Q+H LALK DIP NRISV+NALIAMYSKCGNL+DA+ LF T
Sbjct: 345  SLVCVISACSNMSSPSQGRQVHGLALKLDIPSNRISVNNALIAMYSKCGNLRDAKTLFDT 404

Query: 182  MPEHNTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEG 361
            MPEHNTVS+NS+IAGYAQHG G +SL +FQ+MLE++  PT+ITF+SVL++CAHTG V++G
Sbjct: 405  MPEHNTVSYNSMIAGYAQHGMGFQSLHLFQRMLEMDFTPTNITFISVLAACAHTGRVEDG 464

Query: 362  WKYFNMMKEKFGIEPDAEHYSCMIDLLGRAGKLDEAERLIETMPFDPCSISWGALLGACR 541
              YFNMMK+KFGIEP+A H+SCMIDLLGRAGKL EAERLIET+PFDP    W ALLGACR
Sbjct: 465  KIYFNMMKQKFGIEPEAGHFSCMIDLLGRAGKLSEAERLIETIPFDPGFFXWSALLGACR 524

Query: 542  THGNVELGAKAASHCLRLEPNNAAPYVMLANIYASAGRWEEYATVKRLMRDRGVKKKPGC 721
             HGNVEL  KAA+  L+L+P NAAPYVMLANIY+  GR ++ A+V++LMRDRGVKKKPGC
Sbjct: 525  IHGNVELAIKAANRLLQLDPLNAAPYVMLANIYSDNGRLQDAASVRKLMRDRGVKKKPGC 584

Query: 722  SWIEV 736
            SWIEV
Sbjct: 585  SWIEV 589



 Score = 67.0 bits (162), Expect = 4e-09
 Identities = 51/214 (23%), Positives = 92/214 (42%), Gaps = 32/214 (14%)
 Frame = +2

Query: 2   SFVCVISACSNLSSPSQGKQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQT 181
           SF   +  C        GK LH+L +KS +P +   +SN  + +YSKC  L  ARR+F  
Sbjct: 10  SFRQFLKTCIAHRDLRTGKSLHALYIKSFVPTSTY-LSNHFLLLYSKCRRLSAARRVFDH 68

Query: 182 MPEHNTVSFNSIIAGYAQHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVDEG 361
             + N  SFN++I+ YA+  +   +  +F +M +    P S+++ +++++ A  G     
Sbjct: 69  THDCNVFSFNTLISAYAKESYVEVAHQLFDEMPQ----PDSVSYNTLIAAYARRGDTQPA 124

Query: 362 WKYFNMMKEKF--------------------------------GIEPDAEHYSCMIDLLG 445
           ++ F  M+E F                                G++      + +I    
Sbjct: 125 FQLFLEMREAFLDMDGFTLSGIITACGINVGLIRQLHALSVVTGLDSYVSVGNALITSYS 184

Query: 446 RAGKLDEAERLIETMPFDPCSISWGALLGACRTH 547
           + G L EA R+   +  D   +SW +++ A   H
Sbjct: 185 KNGFLKEARRIFHWLSEDRDEVSWNSMVVAYMQH 218



 Score = 67.0 bits (162), Expect = 4e-09
 Identities = 47/135 (34%), Positives = 77/135 (57%), Gaps = 2/135 (1%)
 Frame = +2

Query: 56  KQLHSLALKSDIPINRISVSNALIAMYSKCGNLQDARRLFQTMPE-HNTVSFNSIIAGYA 232
           +QLH+L++ + +  + +SV NALI  YSK G L++ARR+F  + E  + VS+NS++  Y 
Sbjct: 158 RQLHALSVVTGLD-SYVSVGNALITSYSKNGFLKEARRIFHWLSEDRDEVSWNSMVVAYM 216

Query: 233 QHGHGTKSLLMFQKMLELEVLPTSITFVSVLSSCAHTGMVD-EGWKYFNMMKEKFGIEPD 409
           QH  G+K+L ++ +M    ++    T  SVL+  A T + D  G   F+    K G   +
Sbjct: 217 QHREGSKALELYLEMTVRGLIVDIFTLASVLT--AFTNVQDLLGGLQFHAKLIKSGYHQN 274

Query: 410 AEHYSCMIDLLGRAG 454
           +   S +IDL  + G
Sbjct: 275 SHVGSGLIDLYSKCG 289