BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00021718
         (1627 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   472   e-130
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   457   e-126
gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]               423   e-116
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   417   e-114
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   416   e-114

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  472 bits (1214), Expect = e-130
 Identities = 250/507 (49%), Positives = 325/507 (64%)
 Frame = -3

Query: 1535 MTTLAPSFTIRPQFLKYEAVKSAKLRDAFWSDYQNSGMYDVKRMGTLGSNQFCRETRGIA 1356
            MTTL PS  IRP  +   A  S+KL  A      N   + +++M            +   
Sbjct: 1    MTTLIPSLVIRPCCVNCGARDSSKLVAATHYICGNKVAHGLEKMDLERKEILGVVAQNAT 60

Query: 1355 RISKKELKSMVLHSSQVFSYQVGTDLYSAGIEELEAASTYSFRTDRDNQVKVFIGRKNMT 1176
            R   + +   V  +S +   +      +   EELE+   Y F T+   QVKV +G+KN  
Sbjct: 61   RNCFRNINWKVSATSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGKKNKK 120

Query: 1175 YVVYIEVSSLPEWINENKLVLSWGMYRSDSSSLIPLKPQGSASGSISSINLTPFAQNSLG 996
            Y+V IEVSSL  + ++NKL+LSWG++RS+SS  +P+  Q       S+    PF + S G
Sbjct: 121  YIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSG 180

Query: 995  KHTLQMEFESDQAPFYLSFVLLCPTDAASGKIEMRSHRKEKFCVPVGVGSGHPAPLGISF 816
               L+++FE++ APFYLSF+L    D      ++RSHRK  FC+PVG G G+PAPLG+SF
Sbjct: 181  SFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSF 240

Query: 815  SNDGSVNFALFSRNAETVILCLYDEMEGKPSMEIELDPYINRTGDIWHVSMETAAPYVKY 636
            S+DGS NFA FSRNA  V+LCLYD    KP++EI+LDPY+NRTGDIWH SME+   +V Y
Sbjct: 241  SSDGSPNFAFFSRNAGGVVLCLYDGTSDKPALEIDLDPYVNRTGDIWHASMESVGSFVSY 300

Query: 635  GYRCHGIIPSKKGKTFDESHVLLDPYAKVLQRCFSDHPVSDSLERYLGNLVKDPTFDWSG 456
            GYRC        G+T    HV LDPYAK+++  FSD        R LG L K+P F+W+ 
Sbjct: 301  GYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPR-LGELQKEPAFNWND 359

Query: 455  EIRPCLPMEKLVVYRLNVEHFTKDKSSKLQTGVAGTFSGLIEKSQHFKGLGVNAILLEPI 276
            ++ P +PMEKLVVYRLNV HFTKD+SS++ + +AGTFSGL+EK  HFK LGVNA+LLEPI
Sbjct: 360  DVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI 419

Query: 275  FPFNEQKGPYYPYHFFSPMHLYGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFTHTAE 96
            F F+EQKGPY+P+HFFSPM++YGP+   +S INS+KEMVK +HAN IEVLLEVVFTHTAE
Sbjct: 420  FSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAE 479

Query: 95   CGDYASKTTGLQGIDKKSYYGFNEDME 15
             G        LQGID   YY  N D +
Sbjct: 480  SG-------ALQGIDDSCYYYVNGDAD 499


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  457 bits (1175), Expect = e-126
 Identities = 251/512 (49%), Positives = 323/512 (63%), Gaps = 4/512 (0%)
 Frame = -3

Query: 1535 MTTLAPSFTIRPQFLKYEAVKSAKLRDAFWSDYQNSGMYDVKRMGTLGSNQFCRETRGIA 1356
            M T+ PSF IRP +    A +S+KL          +G  D  R+  L    F +      
Sbjct: 1    MATIIPSFAIRPCYCVCGATESSKLAV--------TGNKDFGRL-VLDQRLFNQRLHFWK 51

Query: 1355 RISKKELKSMVLHSSQVFSYQVGTDLY---SAGIEELEAASTYSFRTDRDNQVKVFIGRK 1185
            +     L S V  +++V   Q  T+L    SA ++EL   STY FRT     VKV + +K
Sbjct: 52   QSPHWSLDSRVCSAARVPVQQ--TELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKK 109

Query: 1184 NMTYVVYIEVSSLPEWINENKLVLSWGMYRSDSSSLIPLKPQGSASGSISSINLTPFAQN 1005
            N  Y VYIEVSSL     + +L+L WG+YRSDSS  +PL  Q  A  +      T   QN
Sbjct: 110  NAKYAVYIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNARKMD--TALVQN 167

Query: 1004 SLGKHTLQMEFESDQAPFYLSFVLLCPTDAASGKIEMRSHRKEKFCVPVGVGSGHPAPLG 825
            S G   L++EFE  Q PFYLSF+L    +  +  +E+++H+   FCVP+G  SG P+PLG
Sbjct: 168  SFGTFALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLG 227

Query: 824  ISFSNDGSVNFALFSRNAETVILCLYDEME-GKPSMEIELDPYINRTGDIWHVSMETAAP 648
            +SFS DGS+NFA FSRN E ++LCLYD+    KP++E++LDPY+NRTGD+WH S+E A  
Sbjct: 228  LSFSTDGSMNFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWT 287

Query: 647  YVKYGYRCHGIIPSKKGKTFDESHVLLDPYAKVLQRCFSDHPVSDSLERYLGNLVKDPTF 468
            +  YGYRC G I        D   VLLDPYA+V+    +DH  S    +YLG L ++P F
Sbjct: 288  FTSYGYRCKGAILQGNTSKVDMECVLLDPYARVIASSMTDHG-SRLSAKYLGRLCEEPAF 346

Query: 467  DWSGEIRPCLPMEKLVVYRLNVEHFTKDKSSKLQTGVAGTFSGLIEKSQHFKGLGVNAIL 288
            +W  +IRP L MEKL+VYRLNV+ FT+ KS KL + +AGTF+GLIEK  HF+ LGVNA+L
Sbjct: 347  EWGSDIRPNLAMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVL 406

Query: 287  LEPIFPFNEQKGPYYPYHFFSPMHLYGPTHDGLSAINSMKEMVKVMHANNIEVLLEVVFT 108
            LEPIFPF+EQKGPY+PYHFFSP ++YGP+   +SAI SMKEMVK +HAN IEVLLEVVFT
Sbjct: 407  LEPIFPFDEQKGPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFT 466

Query: 107  HTAECGDYASKTTGLQGIDKKSYYGFNEDMET 12
            HTAE G        LQGID  SYY     M++
Sbjct: 467  HTAEGG-------ALQGIDDFSYYYTKSSMDS 491


>gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  423 bits (1088), Expect = e-116
 Identities = 216/383 (56%), Positives = 271/383 (70%), Gaps = 1/383 (0%)
 Frame = -3

Query: 1241 TYSFRTDRDNQVKVFIGRKNMTYVVYIEVSSLPEWINENKLVLSWGMYRSDSSSLIPLKP 1062
            TY FRTD   QVKV + R N  Y V +EV  L      ++LV+ WG++RSD+S  +PL  
Sbjct: 87   TYLFRTDIGGQVKVLVERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDASCFMPLDL 146

Query: 1061 QGSASGSISSINLTPFAQNSLGKHTLQMEFESDQAPFYLSFVLLCPTDAASGKIEMRSHR 882
                +   SS   TPF Q   GK T++++FE+  APFY+SF +     +     E+RSHR
Sbjct: 147  NRRGADGKSSTVETPFVQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDMENSEIRSHR 206

Query: 881  KEKFCVPVGVGSGHPAPLGISFSNDGSVNFALFSRNAETVILCLYDEME-GKPSMEIELD 705
               F VPVG+ SGHPAPLGISF  DGSVNFALFSR+A +V+LCLYD++   KPS+EI+LD
Sbjct: 207  NTNFVVPVGLSSGHPAPLGISFQPDGSVNFALFSRSARSVVLCLYDDISVEKPSLEIDLD 266

Query: 704  PYINRTGDIWHVSMETAAPYVKYGYRCHGIIPSKKGKTFDESHVLLDPYAKVLQRCFSDH 525
            PYINR+GDIWH +++ + P+  YGYRC     S KG+      VLLDPYAKV++R     
Sbjct: 267  PYINRSGDIWHAALDCSLPFKTYGYRCKATT-SGKGEL-----VLLDPYAKVIRRVIPRQ 320

Query: 524  PVSDSLERYLGNLVKDPTFDWSGEIRPCLPMEKLVVYRLNVEHFTKDKSSKLQTGVAGTF 345
              S+   +YLG L  +P +DWSG++ P LPMEKL++YRLNV  FTKDKSSKL   +AGTF
Sbjct: 321  GGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLIIYRLNVTQFTKDKSSKLPDDLAGTF 380

Query: 344  SGLIEKSQHFKGLGVNAILLEPIFPFNEQKGPYYPYHFFSPMHLYGPTHDGLSAINSMKE 165
            SG+ EK  HFK LGVNA+LLEPIFPF+EQKGPY+P+HFFSP ++YGP+ D LSAI SMK+
Sbjct: 381  SGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFPWHFFSPGNMYGPSGDPLSAIKSMKD 440

Query: 164  MVKVMHANNIEVLLEVVFTHTAE 96
            MVK +HAN IEV LEVVFTHTAE
Sbjct: 441  MVKKLHANGIEVFLEVVFTHTAE 463


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score =  417 bits (1073), Expect = e-114
 Identities = 220/417 (52%), Positives = 292/417 (70%), Gaps = 5/417 (1%)
 Frame = -3

Query: 1262 EELEAASTYSFRTDRDNQ-VKVFIGRKNMTYVVYIEVSSLP--EWINENKLVLSWGMYRS 1092
            E+L+ +  Y FRT+     VKV + R+N  Y VYIE+SSL     + E  L+L WG+YRS
Sbjct: 79   EDLKGSLAYLFRTETGGGLVKVHVTRRNDRYSVYIEISSLDISGGVGE-ALLLCWGVYRS 137

Query: 1091 DSSSLIPLKPQGSASGSISSINLTPFAQNSLGKHTLQMEFESDQAPFYLSFVLLCPTDAA 912
            DSS  + L   G +  +   +N++P  QNS GK  +++EF++   P YLSF L+   D+ 
Sbjct: 138  DSSCFVDLDTIGLSENAAMGMNVSPLVQNSDGKFAIELEFDAKHVPLYLSFFLMSSLDSG 197

Query: 911  SGKIEMRSHRKEKFCVPVGVGSGHPAPLGISFSNDGSVNFALFSRNAETVILCLYDEME- 735
               +E+RSHR+  FC+PVG   G+P PLG+S+S DGSVNF++FSR+AE+V+LCLYDE   
Sbjct: 198  ---LEIRSHRRTNFCMPVGSLPGYPCPLGVSYSPDGSVNFSIFSRHAESVVLCLYDENGV 254

Query: 734  GKPSMEIELDPYINRTGDIWHVSMETAAPYVKYGYRCH-GIIPSKKGKTFDESHVLLDPY 558
             KP++E++LDPY+NRTGDIWHVS E+A  +V YGYRC  G++   K   F E HV+LDPY
Sbjct: 255  EKPALELDLDPYVNRTGDIWHVSFESAKGFVSYGYRCRRGVLKKNKDDGFAE-HVVLDPY 313

Query: 557  AKVLQRCFSDHPVSDSLERYLGNLVKDPTFDWSGEIRPCLPMEKLVVYRLNVEHFTKDKS 378
            AK++   + D      L + LG L K+P FDW G+  P L MEKLVVYRLNV+ FT+ +S
Sbjct: 314  AKIVGNSYPD---GVGLVKNLGCLRKEPFFDWGGDRHPDLSMEKLVVYRLNVKRFTQHES 370

Query: 377  SKLQTGVAGTFSGLIEKSQHFKGLGVNAILLEPIFPFNEQKGPYYPYHFFSPMHLYGPTH 198
            S+L +G+AGTF+GL +K QHFK LGVNA+LLEP+F F+E+KGPY+P HFFS MH+YGP+ 
Sbjct: 371  SQLPSGLAGTFTGLAKKVQHFKDLGVNAVLLEPVFTFDEKKGPYFPCHFFSLMHIYGPSG 430

Query: 197  DGLSAINSMKEMVKVMHANNIEVLLEVVFTHTAECGDYASKTTGLQGIDKKSYYGFN 27
              +SAI +MKEMVK MHAN IEVL+EVVF++TAE G        +QGID  SYY  N
Sbjct: 431  GPVSAIAAMKEMVKTMHANGIEVLVEVVFSNTAEIG-------AIQGIDDSSYYYAN 480


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score =  416 bits (1070), Expect = e-114
 Identities = 238/520 (45%), Positives = 312/520 (60%), Gaps = 17/520 (3%)
 Frame = -3

Query: 1535 MTTLAPSFTIRPQFLKYEA--VKSAKLRDAFWSDYQNSGMY-----DVKRMGTLGSNQFC 1377
            M T   S  I+P F  Y     +S KL  +    Y     Y     D  RM   G N+  
Sbjct: 1    MATFLSSLVIQP-FCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVG 59

Query: 1376 RETRGIARISKKELKSMVLHSSQVFSYQVGTDLYSAGI----EELEAASTYSFRTDRDNQ 1209
                    +SK   KS +          VG      GI    +E    +TY FRT+  + 
Sbjct: 60   AVKSSHRNLSKTYAKSGI---------SVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDL 110

Query: 1208 VKVFIGRKNMTYVVYIEVSSLPEWINENKLVLSWGMYRSDSSSLIPLKPQGSASGSISSI 1029
            V VF+G+K  T+ V IEV S+     +  L+LSWG+YRSDS+ + P   + S     +  
Sbjct: 111  VNVFVGKKGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGA 169

Query: 1028 NLTPFAQNSLGKHTLQMEFESDQAPFYLSFVLLCPTDAASGKIEMRSHRKEKFCVPVGVG 849
              TPF + S GK ++++EF++   PFYLSFVL  P    SG  E+RSH+K  F VPVG G
Sbjct: 170  TETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFG 229

Query: 848  SGHPAPLGISFSNDGSVNFALFSRNAETVILCLY-DEMEGKPSMEIELDPYINRTGDIWH 672
             G+P+PLG+S S DGSVNF++FS +AE+++LCLY D    KP +E++LDPYINR+G+IWH
Sbjct: 230  RGYPSPLGLSISGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWH 289

Query: 671  VSMETAAPYVKYGYRCHGIIPSKKGKTFDESHVLLDPYAKVLQRCFSDHPVSDSL----- 507
             S E A+ +V YGY+C G    +     + S +++DPYAK+L       P S        
Sbjct: 290  ASFEGASKFVSYGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSI---PKSSGQGLGLP 346

Query: 506  ERYLGNLVKDPTFDWSGEIRPCLPMEKLVVYRLNVEHFTKDKSSKLQTGVAGTFSGLIEK 327
             ++LG + K PTFDW GE+ P LPMEKL VYRLNVE FT DKSS+L   +AGTFSGL +K
Sbjct: 347  SKFLGQISKVPTFDWDGEVHPNLPMEKLXVYRLNVERFTMDKSSQLPADIAGTFSGLTKK 406

Query: 326  SQHFKGLGVNAILLEPIFPFNEQKGPYYPYHFFSPMHLYGPTHDGLSAINSMKEMVKVMH 147
              HFK LGVNA+LLEPIF F+E++GPY+P+HFFSP + YGP+   +SAINSMKEMVK +H
Sbjct: 407  LLHFKNLGVNAVLLEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELH 466

Query: 146  ANNIEVLLEVVFTHTAECGDYASKTTGLQGIDKKSYYGFN 27
            AN +EV+LEVV+THT+  G        LQGID  SYY  N
Sbjct: 467  ANGVEVILEVVYTHTSGNG-------ALQGIDDSSYYFTN 499