BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00020344
         (1571 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      535   e-149
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   510   e-142
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       496   e-138
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   445   e-122
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   414   e-113

>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  535 bits (1378), Expect = e-149
 Identities = 257/355 (72%), Positives = 300/355 (84%)
 Frame = +2

Query: 347  EAKQPKKAATRXXXXXXXXXXXPYDDIKQLIRIELAKRLQCGYKSTCEEQVAEVLKLVHS 526
            EAKQ KKAA             PYDDIKQLIR ELA+RLQ GYK T EEQ+AE+  L HS
Sbjct: 4    EAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNLTHS 63

Query: 527  LQHMKIANEVETLDSQLYEPPMSFLKIIFGSTIKGSCCYFQDDSMTLDDAEIAMLDLYCE 706
            L+ MKIA EVETLDSQLYE P+ FLKI+ GS +KGSCCYF++DS TLD+AEIAMLDLYCE
Sbjct: 64   LRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDLYCE 123

Query: 707  RAQIKDGNSVLDLGCGQGALTLFVAQKYKQCRVKAITNSISQKEYIDEQCRTRNLVNVEV 886
            RAQI+DG SVLDLGCGQGALTL VAQKYK CRV A+TNS+SQKEYI+E+ R RNL+NVEV
Sbjct: 124  RAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLNVEV 183

Query: 887  ILADITTYEMDDTSDRILAIGLFEHMKNYKLLLKKISKWMAEDGLLFVDHVCHKTLAYQY 1066
             LADITT+EM +T DRIL I LFEHMKNY+LLL+KIS+W+++DGLLF++H+CHKT AY Y
Sbjct: 184  KLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFAYHY 243

Query: 1067 KALDEDDWFTEYIFPAETIIFPSASFLLYFQDDVSVVNHWILNGNHFSQTHGEWLKRLDA 1246
            + LD+DDWFTEY+FPA T+I PSASF LYFQDDVSVVNHW L+G HFS+T+ EWLKRLDA
Sbjct: 244  EPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDA 303

Query: 1247 NVAAIKQMFKSLTGNDEEALKMINFWRIFCLFGIELFGYNNGEEWMTSHVLFKKK 1411
            N+  IK MF++L GN+EEA+K+IN+WR FCL G+E+FGYNNGEEWM SHVLFKKK
Sbjct: 304  NLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 358


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  510 bits (1314), Expect = e-142
 Identities = 247/358 (68%), Positives = 296/358 (82%), Gaps = 3/358 (0%)
 Frame = +2

Query: 347  EAKQ--PKKAATRXXXXXXXXXXXPYDDIKQLIRIELAKRLQCGYKSTCEEQVAEVLKLV 520
            E KQ  PKKA              P D+IK+LIRI+L +RLQ G KST EEQ+A+++ L 
Sbjct: 4    EGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLT 63

Query: 521  HSLQHMKIANEVETLDSQLYEPPMSFLKIIFGSTIKGSCCYFQDDSMTLDDAEIAMLDLY 700
            HSL+ MKIA EVETLD Q+YE P+ FLKI+ GS +KGSCCYF++DS TLD+AEIAML+LY
Sbjct: 64   HSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELY 123

Query: 701  CERAQIKDGNSVLDLGCGQGALTLFVAQKYKQCRVKAITNSISQKEYIDEQCRTRNLVNV 880
            CERAQIKDG+SVLDLGCGQGALTL+VAQKYK  RV A+TNS+SQKE+I+E+ R RNL NV
Sbjct: 124  CERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNV 183

Query: 881  EVILADITTYEMDDTSDRILAIGLFEHMKNYKLLLKKISKWMAEDGLLFVDHVCHKTLAY 1060
            EV+LADITT++M DT DRIL + LFEHMKNY+LLL+KI +WMA+DGLLFV+H+CHKT AY
Sbjct: 184  EVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAY 243

Query: 1061 QYKALDEDDWFTEYIFPAETIIFPSASFLLYFQDDVSVVNHWILNGNHFSQTHGEWLKRL 1240
             Y+ +DEDDWFTEY+FPA T+I PSASF LYFQDDVSVVNHW L+G HFS+T+ EWLKRL
Sbjct: 244  HYEPIDEDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRL 303

Query: 1241 DANVAAIKQMFKSLTGN-DEEALKMINFWRIFCLFGIELFGYNNGEEWMTSHVLFKKK 1411
            DANV  IK MF ++TG   +EA+K+IN+WR FCL G+E+FGYNNGEEWM SHVLFKKK
Sbjct: 304  DANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKKK 361


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  496 bits (1276), Expect = e-138
 Identities = 233/333 (69%), Positives = 281/333 (84%)
 Frame = +2

Query: 413  PYDDIKQLIRIELAKRLQCGYKSTCEEQVAEVLKLVHSLQHMKIANEVETLDSQLYEPPM 592
            P D++K+L+RI+LA+RLQ GYKST EEQ+A+VL L HSL+HM IA EV+TLDS +YE P+
Sbjct: 19   PNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVDTLDSNMYEVPL 78

Query: 593  SFLKIIFGSTIKGSCCYFQDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTL 772
             FLKI+ GS +K S CYF+DDS TLD+AEIAM+DL+CERAQIKDG+SVLDLGCG G+LT 
Sbjct: 79   DFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVLDLGCGHGSLTF 138

Query: 773  FVAQKYKQCRVKAITNSISQKEYIDEQCRTRNLVNVEVILADITTYEMDDTSDRILAIGL 952
            +VAQKYK+  V A+TNS+SQKEYI+E+ R RNL NVEV+LADI T+EM DT DRIL +GL
Sbjct: 139  YVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMADTYDRILVVGL 198

Query: 953  FEHMKNYKLLLKKISKWMAEDGLLFVDHVCHKTLAYQYKALDEDDWFTEYIFPAETIIFP 1132
            FEHMKNY LLLKKIS+WMA+DGLLFV HVCHKT A+  + +DEDDW +EY FPA T+I P
Sbjct: 199  FEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSEYAFPAGTMIIP 258

Query: 1133 SASFLLYFQDDVSVVNHWILNGNHFSQTHGEWLKRLDANVAAIKQMFKSLTGNDEEALKM 1312
            SASF LYFQDD SVVNHW L+G HFS+T  EWLKRLDANV  I  MF ++TG++EEA+K+
Sbjct: 259  SASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFATITGSEEEAVKL 318

Query: 1313 INFWRIFCLFGIELFGYNNGEEWMTSHVLFKKK 1411
            +N+WR  C FG+EL+GYNNGEEWM SHVLFKKK
Sbjct: 319  LNYWRGLCFFGVELYGYNNGEEWMESHVLFKKK 351


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  445 bits (1145), Expect = e-122
 Identities = 207/333 (62%), Positives = 265/333 (79%)
 Frame = +2

Query: 413  PYDDIKQLIRIELAKRLQCGYKSTCEEQVAEVLKLVHSLQHMKIANEVETLDSQLYEPPM 592
            P  +I+QLIR+EL KRLQ GYK T EEQ++++L LVHSL+ MK+A E+E LD +LYE PM
Sbjct: 19   PDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENLDLKLYEAPM 78

Query: 593  SFLKIIFGSTIKGSCCYFQDDSMTLDDAEIAMLDLYCERAQIKDGNSVLDLGCGQGALTL 772
             FLKI  GS +K S  Y+ D+S TLD+AEIAMLDLY ERAQIKDG SVLDLGCG GA+ L
Sbjct: 79   EFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDLGCGLGAVAL 138

Query: 773  FVAQKYKQCRVKAITNSISQKEYIDEQCRTRNLVNVEVILADITTYEMDDTSDRILAIGL 952
            F A K+K+C+   +T+S+ QK+YI+ +C+   L NV+V+LADITTYE ++  DRI A+ L
Sbjct: 139  FGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEERFDRIFAVEL 198

Query: 953  FEHMKNYKLLLKKISKWMAEDGLLFVDHVCHKTLAYQYKALDEDDWFTEYIFPAETIIFP 1132
             EHMKNY+LLLKKIS+WM +DGLLFV+HVCHKTLAY Y+ +D +DW+T YIFPA T+   
Sbjct: 199  IEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYIFPAGTLTLS 258

Query: 1133 SASFLLYFQDDVSVVNHWILNGNHFSQTHGEWLKRLDANVAAIKQMFKSLTGNDEEALKM 1312
            SAS LLYFQDDVSVVN W L+G H+S++H EWLK +D N+   K++ +S+T  ++EA+K+
Sbjct: 259  SASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIMRSITKTEKEAIKL 318

Query: 1313 INFWRIFCLFGIELFGYNNGEEWMTSHVLFKKK 1411
            +NFWRIFC+ G ELFGY NGEEWM +H+LFKKK
Sbjct: 319  LNFWRIFCMCGAELFGYKNGEEWMLTHLLFKKK 351


>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  414 bits (1065), Expect = e-113
 Identities = 194/355 (54%), Positives = 265/355 (74%)
 Frame = +2

Query: 347  EAKQPKKAATRXXXXXXXXXXXPYDDIKQLIRIELAKRLQCGYKSTCEEQVAEVLKLVHS 526
            E KQ KK A               ++I+ +++I++ KRL+ GYK T E+Q+A+++    S
Sbjct: 2    ETKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQS 61

Query: 527  LQHMKIANEVETLDSQLYEPPMSFLKIIFGSTIKGSCCYFQDDSMTLDDAEIAMLDLYCE 706
            L+ M++A EV+TLD++LYE P+ FL I+ G T+K S  YF+D+S TLD++E+ M+DLYCE
Sbjct: 62   LKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCE 121

Query: 707  RAQIKDGNSVLDLGCGQGALTLFVAQKYKQCRVKAITNSISQKEYIDEQCRTRNLVNVEV 886
            RAQIKDG S+LDLGCG G+LTL VAQKY+ C+V  ITNS+SQKE+I +QC+  +L NVE+
Sbjct: 122  RAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEI 181

Query: 887  ILADITTYEMDDTSDRILAIGLFEHMKNYKLLLKKISKWMAEDGLLFVDHVCHKTLAYQY 1066
            IL D+T +E + T DRI A+ L EHMKNY+L LKK+S W+A+ GLLFV+H CHK  AYQY
Sbjct: 182  ILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQY 241

Query: 1067 KALDEDDWFTEYIFPAETIIFPSASFLLYFQDDVSVVNHWILNGNHFSQTHGEWLKRLDA 1246
            + LDEDDW+TEYIFP+ T++  S+S LLYFQ+DVSVVNHW L+G H S    +WLKRLD 
Sbjct: 242  EPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDD 301

Query: 1247 NVAAIKQMFKSLTGNDEEALKMINFWRIFCLFGIELFGYNNGEEWMTSHVLFKKK 1411
            N+  +K++F+S  G+ E+A+K I +WR+FC+   +++  NNGEEWM S VLFKKK
Sbjct: 302  NIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356