BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00018150
         (1534 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid ...   651   0.0  
ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinu...   639   0.0  
gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]       633   e-179
ref|XP_002265630.1| PREDICTED: ent-kaurenoic acid oxidase 2 [Vit...   633   e-179
ref|XP_002319447.1| cytochrome P450 probable ent-kaurenoic acid ...   630   e-178

>ref|XP_002321248.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222862021|gb|EEE99563.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 493

 Score =  651 bits (1680), Expect = 0.0
 Identities = 306/433 (70%), Positives = 371/433 (85%), Gaps = 1/433 (0%)
 Frame = +3

Query: 237  KKANEWYYESKLGEKRLSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGM 416
            KK N W YE++LGEK+ SLPPGD+GWPFIGNMWSFLRAFKS+DP+SF+  F+ ++G  G+
Sbjct: 25   KKVNWWLYEAQLGEKQYSLPPGDLGWPFIGNMWSFLRAFKSSDPDSFMRTFINKYGDNGI 84

Query: 417  YKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRI 596
            YKAFMFGNP++ VT PE CR+VL +D+ FKPGWP ST++LIGRKSF+ +SYE+HK LRR+
Sbjct: 85   YKAFMFGNPSVFVTTPEACRRVLSNDDAFKPGWPISTLKLIGRKSFIDISYEEHKRLRRL 144

Query: 597  TAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGES 776
            T+APVNGHEALSVY+ +IEENV++ LE+W+ MG+IEFLTQ+RKLTF+I+M+IF+S   E 
Sbjct: 145  TSAPVNGHEALSVYIPYIEENVIAMLEKWTTMGKIEFLTQVRKLTFKIIMYIFLSSESEV 204

Query: 777  VMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPA 956
            VM+ALE+ YTTLNYGVRAMAINLPGFAYY+ALK+RKRLV IF S+V+ERR +R      A
Sbjct: 205  VMEALEKDYTTLNYGVRAMAINLPGFAYYKALKARKRLVAIFQSIVDERRNLRKNSARNA 264

Query: 957  -KKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQK 1133
             KKDM+D+LL V+DEN RKL DEEIID ++MYLNAGHESSGHIT WAT+ LQEHP+  QK
Sbjct: 265  KKKDMMDSLLGVEDENGRKLTDEEIIDVILMYLNAGHESSGHITTWATIFLQEHPEFLQK 324

Query: 1134 AKAEQEQIVKNRSSMQKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGY 1313
            AK EQEQIVK R   Q GL LKE+++MDYL+KVIDETLRLI+FSL VFREAK D S+NGY
Sbjct: 325  AKEEQEQIVKRRPPAQNGLSLKEVREMDYLSKVIDETLRLITFSLTVFREAKTDFSINGY 384

Query: 1314 IIPMGWKVEVWLRNVHRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAK 1493
            IIP GWKV VW R VH DPE+Y NP +F+PSRWD +T KAGT+LPFGAGSRLCPGN+LAK
Sbjct: 385  IIPKGWKVLVWFRTVHLDPEIYQNPKEFNPSRWDNYTPKAGTFLPFGAGSRLCPGNDLAK 444

Query: 1494 LEIAIFMYYFLLN 1532
            LEI+IF++YFLL+
Sbjct: 445  LEISIFLHYFLLD 457


>ref|XP_002526524.1| Ent-kaurenoic acid oxidase, putative [Ricinus communis]
            gi|223534199|gb|EEF35915.1| Ent-kaurenoic acid oxidase,
            putative [Ricinus communis]
          Length = 492

 Score =  639 bits (1647), Expect = 0.0
 Identities = 299/434 (68%), Positives = 365/434 (84%), Gaps = 2/434 (0%)
 Frame = +3

Query: 237  KKANEWYYESKLGEKRLSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGM 416
            K+ N W YE++LGE + SLPPGD+GWPFIGNMWSFLRAFKSNDP+SF+  F  R+G  G+
Sbjct: 25   KRVNCWLYENQLGEMQYSLPPGDLGWPFIGNMWSFLRAFKSNDPDSFMRNFTARYGSGGI 84

Query: 417  YKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRI 596
            YKAFMFG P+++VT  E C++VL DD+ FKPGWP ST ELIG+KSF+G+SYE+HK LRR+
Sbjct: 85   YKAFMFGKPSVLVTTSEACKRVLTDDDAFKPGWPSSTTELIGKKSFIGISYEEHKRLRRL 144

Query: 597  TAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGES 776
            TA+PVNGHEALSVY+ +IE+ V S LE+WS MGEI+FLTQLRKLTFRI+M+IF+S    S
Sbjct: 145  TASPVNGHEALSVYMHYIEDKVKSALEKWSTMGEIQFLTQLRKLTFRIIMYIFLSSESHS 204

Query: 777  VMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPA 956
            VM+ALE  YTTLNYGVRAMAINLPGFAYY+ALK+RK LV +   VV+ RR  +    P +
Sbjct: 205  VMEALEREYTTLNYGVRAMAINLPGFAYYKALKARKNLVAVLQFVVDARRNQKKGAEPNS 264

Query: 957  --KKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQ 1130
              KKDM+D LLDV+DE  RKL+DEEI+D L+MYLNAGHESSGHITMWATV LQEHP+  Q
Sbjct: 265  SKKKDMMDALLDVEDEKGRKLSDEEIVDVLLMYLNAGHESSGHITMWATVFLQEHPEFLQ 324

Query: 1131 KAKAEQEQIVKNRSSMQKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNG 1310
            KA+ EQE+I+K R   QKGL LKE++ M+YL+KVIDETLRLI+FSLVVFREAK +V+++G
Sbjct: 325  KARQEQEEIIKRRPPTQKGLTLKEVRDMEYLSKVIDETLRLITFSLVVFREAKTNVNISG 384

Query: 1311 YIIPMGWKVEVWLRNVHRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELA 1490
            Y+IP GWK+ VW R+VH DPE+YPNP +F+PSRWD  T KAGT+LPFGAGSR+CPGN+LA
Sbjct: 385  YVIPKGWKILVWFRSVHLDPEIYPNPREFNPSRWDNHTAKAGTFLPFGAGSRMCPGNDLA 444

Query: 1491 KLEIAIFMYYFLLN 1532
            KLEIAIF+++FLLN
Sbjct: 445  KLEIAIFLHHFLLN 458


>gb|AEF32085.1| ent-kaurenoic acid oxidase [Castanea mollissima]
          Length = 492

 Score =  633 bits (1633), Expect = e-179
 Identities = 295/432 (68%), Positives = 363/432 (84%)
 Frame = +3

Query: 237  KKANEWYYESKLGEKRLSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGM 416
            K AN W YE++LGEK+ SLPPGD+GWPFIGNMWSFL AFKS DP+SF++ FV RFGR G+
Sbjct: 25   KNANWWLYETQLGEKQYSLPPGDLGWPFIGNMWSFLSAFKSKDPDSFVSSFVSRFGRTGI 84

Query: 417  YKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRI 596
            YK FMFGNP+++VT PE CR+VL DD+ FKPGWP+STV LIG+KSF+G+S+E+HK LRR+
Sbjct: 85   YKVFMFGNPSVIVTSPEACRRVLSDDDCFKPGWPKSTVALIGKKSFIGISFEEHKRLRRL 144

Query: 597  TAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGES 776
            TAAPVNG+EALS+Y ++IEE V S+L++W+ MGEIEFLT+LRKLTFRI+M+IF+S   E 
Sbjct: 145  TAAPVNGYEALSMYTKYIEEIVTSSLDKWTTMGEIEFLTELRKLTFRIIMYIFLSSESEP 204

Query: 777  VMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPA 956
            VM ALE  YT LNYGVRAMAIN+PGFAY++ALK+RK LV  F S+V++RR  +    P  
Sbjct: 205  VMTALEREYTALNYGVRAMAINIPGFAYHKALKARKNLVATFQSIVDDRRNQKKVSVPTK 264

Query: 957  KKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKA 1136
            KKDM+D L+DVKDEN RKL DEEIID L+MYLNAGHESSGH  MWAT+ L +HP+  QKA
Sbjct: 265  KKDMMDALIDVKDENGRKLTDEEIIDILVMYLNAGHESSGHTAMWATIFLNQHPEYLQKA 324

Query: 1137 KAEQEQIVKNRSSMQKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYI 1316
            K EQE+IV+ R   QKGL LKEI++M+YL+KVIDETLR+I+FSL VFREAK+DV++ GY 
Sbjct: 325  KKEQEEIVRKRLPEQKGLTLKEIREMEYLSKVIDETLRVITFSLTVFREAKQDVNIAGYT 384

Query: 1317 IPMGWKVEVWLRNVHRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKL 1496
            IP GW+V VW R+VH DPE+Y NP +F PSRWD FT KAG +LPFGAG+RLCPGN+LAKL
Sbjct: 385  IPKGWRVLVWFRSVHLDPEIYENPKEFKPSRWDNFTPKAGAFLPFGAGTRLCPGNDLAKL 444

Query: 1497 EIAIFMYYFLLN 1532
            EI+IF+++FLLN
Sbjct: 445  EISIFLHHFLLN 456


>ref|XP_002265630.1| PREDICTED: ent-kaurenoic acid oxidase 2 [Vitis vinifera]
          Length = 492

 Score =  633 bits (1632), Expect = e-179
 Identities = 292/432 (67%), Positives = 363/432 (84%)
 Frame = +3

Query: 237  KKANEWYYESKLGEKRLSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGM 416
            ++AN W YE KLGEKR SLPPGD+GWP IGNMWSFLRAFKS DP+SFI+ F+ RFG+ GM
Sbjct: 25   RRANSWVYEVKLGEKRYSLPPGDLGWPLIGNMWSFLRAFKSTDPDSFISSFITRFGQTGM 84

Query: 417  YKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRI 596
            YK  MFGNP+I+VTIPE C++VL DD+ FKPGWP ST+ELIGRKSF+G++ E+HK LRR+
Sbjct: 85   YKVLMFGNPSIIVTIPEACKRVLTDDQNFKPGWPTSTMELIGRKSFIGITNEEHKRLRRL 144

Query: 597  TAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGES 776
            TA PVNGHEALS+Y+++IE+NV+S L +W+ MGE EFLT LRKLTF+I+M+IF+S   E 
Sbjct: 145  TATPVNGHEALSIYMQYIEDNVISALNKWAAMGEFEFLTALRKLTFKIIMYIFLSSESEH 204

Query: 777  VMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRDGPPA 956
            VM+ALE  YT+LNYGVR+MAINLPGFAY++ALK+RK LV IF S+VNERR  +  +    
Sbjct: 205  VMEALEREYTSLNYGVRSMAINLPGFAYHKALKARKNLVNIFQSIVNERRDRKKGNSQTM 264

Query: 957  KKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQKA 1136
            KKDM+D LLD++DEN RKL+DEEIID L+MYLNAGHESS H+TMWATV LQE+P+ FQ+A
Sbjct: 265  KKDMMDALLDIEDENGRKLSDEEIIDILVMYLNAGHESSAHVTMWATVKLQENPEFFQRA 324

Query: 1137 KAEQEQIVKNRSSMQKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGYI 1316
            KAEQE+I++ R   QK L LKEI++M+YL KVIDETLR I+FS VVFREAK D+++ GY 
Sbjct: 325  KAEQEEIIRKRPPNQKRLTLKEIREMEYLPKVIDETLRWITFSFVVFREAKADINICGYT 384

Query: 1317 IPMGWKVEVWLRNVHRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAKL 1496
            IP GWKV VW R++H DPE YP+P +F+P RWD +T K GT+LPFG GSRLCPGN+LAKL
Sbjct: 385  IPKGWKVLVWFRSLHFDPETYPDPKEFNPCRWDDYTAKPGTFLPFGLGSRLCPGNDLAKL 444

Query: 1497 EIAIFMYYFLLN 1532
            EI++F+++FLLN
Sbjct: 445  EISVFLHHFLLN 456


>ref|XP_002319447.1| cytochrome P450 probable ent-kaurenoic acid oxidase [Populus
            trichocarpa] gi|222857823|gb|EEE95370.1| cytochrome P450
            probable ent-kaurenoic acid oxidase [Populus trichocarpa]
          Length = 490

 Score =  630 bits (1626), Expect = e-178
 Identities = 292/433 (67%), Positives = 362/433 (83%), Gaps = 1/433 (0%)
 Frame = +3

Query: 237  KKANEWYYESKLGEKRLSLPPGDMGWPFIGNMWSFLRAFKSNDPESFINGFVKRFGRIGM 416
            K+ N W YE+KLG K+ SLPPGD+GWPFIGNMWSFL AFKS+DP+SFI  FV R+G  G+
Sbjct: 25   KRVNWWLYEAKLGAKKDSLPPGDLGWPFIGNMWSFLTAFKSSDPDSFIRSFVNRYGHTGI 84

Query: 417  YKAFMFGNPTIMVTIPETCRQVLMDDEKFKPGWPQSTVELIGRKSFVGLSYEDHKWLRRI 596
            YKAFMFGNP+++VT PE CR++L DD  FKPGWP +T++LIG KSF+ + YE+HK LRR+
Sbjct: 85   YKAFMFGNPSVLVTTPEGCRRLLTDDNAFKPGWPLATLKLIGEKSFIDIPYEEHKRLRRL 144

Query: 597  TAAPVNGHEALSVYLEFIEENVVSTLERWSNMGEIEFLTQLRKLTFRIVMHIFISKGGES 776
            T+A VNGHEALS Y+ +IE+NV++ LE+W+ MG+IEFLT++RKLTFRI+++IF+SK  E 
Sbjct: 145  TSASVNGHEALSTYIPYIEQNVIAELEKWTTMGQIEFLTKMRKLTFRIIIYIFLSKTSER 204

Query: 777  VMDALEEVYTTLNYGVRAMAINLPGFAYYRALKSRKRLVEIFLSVVNERRAVRVRD-GPP 953
            VM+ALE+ YTTLNYG+RAMAINLPGFAYY ALK+RK+LV IF S+V+ RR ++  D    
Sbjct: 205  VMEALEKEYTTLNYGIRAMAINLPGFAYYEALKARKKLVAIFQSIVDGRRNLKKDDVTNT 264

Query: 954  AKKDMLDNLLDVKDENDRKLNDEEIIDTLIMYLNAGHESSGHITMWATVLLQEHPDMFQK 1133
             KKDM+D+LLDV+DEN RKL DEE+ID ++MYLNAGHESSGHIT WAT+ LQ+HP+ FQK
Sbjct: 265  KKKDMMDSLLDVEDENGRKLTDEEVIDIMLMYLNAGHESSGHITTWATIFLQDHPEYFQK 324

Query: 1134 AKAEQEQIVKNRSSMQKGLRLKEIKQMDYLNKVIDETLRLISFSLVVFREAKKDVSMNGY 1313
            AK EQEQI+K R   QK L LKE+++M YL+KVIDETLR+++FSL VFREAK D  MNGY
Sbjct: 325  AKEEQEQIIKRRPLTQKRLSLKEVREMKYLSKVIDETLRMVTFSLTVFREAKTDFCMNGY 384

Query: 1314 IIPMGWKVEVWLRNVHRDPEVYPNPNKFDPSRWDGFTQKAGTYLPFGAGSRLCPGNELAK 1493
             IP GWKV  W R +H DPEVYPNP +F+PSRWD +T KAGT+LPFGAGSRLCPGN LAK
Sbjct: 385  TIPKGWKVLAWFRTIHLDPEVYPNPKEFNPSRWDDYTPKAGTFLPFGAGSRLCPGNNLAK 444

Query: 1494 LEIAIFMYYFLLN 1532
            LEI+IF++YFLL+
Sbjct: 445  LEISIFLHYFLLD 457