BLASTX nr result
ID: Coptis24_contig00016743
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00016743
(1179 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002281826.1| PREDICTED: isocitrate dehydrogenase [NAD] ca... 333 4e-89
emb|CBI32749.3| unnamed protein product [Vitis vinifera] 333 4e-89
ref|XP_002519520.1| isocitrate dehydrogenase, putative [Ricinus ... 331 2e-88
ref|XP_003603020.1| Isocitrate dehydrogenase [Medicago truncatul... 329 7e-88
ref|XP_004143261.1| PREDICTED: isocitrate dehydrogenase [NAD] ca... 329 9e-88
>ref|XP_002281826.1| PREDICTED: isocitrate dehydrogenase [NAD] catalytic subunit 5,
mitochondrial-like [Vitis vinifera]
Length = 367
Score = 333 bits (855), Expect = 4e-89
Identities = 174/315 (55%), Positives = 219/315 (69%), Gaps = 18/315 (5%)
Frame = +3
Query: 288 SSPFITNISTSANADPSPSYPIQTTLFPGLGIGPDITESVKQVLSTAEVPIKWEEHFVGN 467
SS ++ + S ++ S S I+ TLFPG GIGP+I E+VKQV A+VPI+WEEH+VG+
Sbjct: 16 SSRAFSSFNRSFSSSDSSSNLIRATLFPGDGIGPEIAEAVKQVFRAADVPIEWEEHYVGD 75
Query: 468 KEDQRTKSYLTWESLESIRKNGAGIKAPMWKPIGNAQRSIYHAFREELSLYASVKLFNSL 647
+ D RT+S+LTWESLES+R+NG G+K PM PIG RS+ R+EL+LYA+V+ SL
Sbjct: 76 QIDPRTQSFLTWESLESVRQNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSL 135
Query: 648 YGYKPRKNDVDIVTVT------------QQMRGVDES------QVCAHVAEYAFYYARTH 773
GYK R +DVD++T+ Q +RGV ES Q VAEYAF+YA+TH
Sbjct: 136 PGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTH 195
Query: 774 GRDKVSAIHEPEITTKTDGLFLKCCREVANKYPEIRYEEITREACVIKLATDHAYFDVLA 953
GR++VSAIH+ I KTDGLFLKCCREVA KYPEI+YEE+ + C + L + A FDVL
Sbjct: 196 GRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLV 255
Query: 954 VDRFSDYSVSNDCAALVGGLRLVPSCNIGEGGIALAEAVPGTEPDFTEKNLANPTPLLLS 1133
+ +S+ CA L+GGL L PSCNIGEGGIALAEAV G+ PD KN+ANPT L+LS
Sbjct: 256 MPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALMLS 315
Query: 1134 TVMMLHHLKLHDKAD 1178
V ML HLKLHDKAD
Sbjct: 316 AVTMLRHLKLHDKAD 330
Score = 134 bits (336), Expect = 6e-29
Identities = 63/89 (70%), Positives = 77/89 (86%)
Frame = +3
Query: 3 PSCNIGEGNIALAEAVPGTTPEIDDMNLANPTPLLLSAVMMLQHLKLHDKADQIHDAIIK 182
PSCNIGEG IALAEAV G+ P+I N+ANPT L+LSAV ML+HLKLHDKADQI +AI+
Sbjct: 279 PSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALMLSAVTMLRHLKLHDKADQIQNAILN 338
Query: 183 TIAEGKYQTADIGGTSTTKDVTKSICNHL 269
TI+EG+Y+TAD+GG+STT D TK+IC+HL
Sbjct: 339 TISEGRYRTADLGGSSTTTDFTKAICDHL 367
>emb|CBI32749.3| unnamed protein product [Vitis vinifera]
Length = 366
Score = 333 bits (855), Expect = 4e-89
Identities = 174/315 (55%), Positives = 219/315 (69%), Gaps = 18/315 (5%)
Frame = +3
Query: 288 SSPFITNISTSANADPSPSYPIQTTLFPGLGIGPDITESVKQVLSTAEVPIKWEEHFVGN 467
SS ++ + S ++ S S I+ TLFPG GIGP+I E+VKQV A+VPI+WEEH+VG+
Sbjct: 15 SSRAFSSFNRSFSSSDSSSNLIRATLFPGDGIGPEIAEAVKQVFRAADVPIEWEEHYVGD 74
Query: 468 KEDQRTKSYLTWESLESIRKNGAGIKAPMWKPIGNAQRSIYHAFREELSLYASVKLFNSL 647
+ D RT+S+LTWESLES+R+NG G+K PM PIG RS+ R+EL+LYA+V+ SL
Sbjct: 75 QIDPRTQSFLTWESLESVRQNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSL 134
Query: 648 YGYKPRKNDVDIVTVT------------QQMRGVDES------QVCAHVAEYAFYYARTH 773
GYK R +DVD++T+ Q +RGV ES Q VAEYAF+YA+TH
Sbjct: 135 PGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTH 194
Query: 774 GRDKVSAIHEPEITTKTDGLFLKCCREVANKYPEIRYEEITREACVIKLATDHAYFDVLA 953
GR++VSAIH+ I KTDGLFLKCCREVA KYPEI+YEE+ + C + L + A FDVL
Sbjct: 195 GRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLV 254
Query: 954 VDRFSDYSVSNDCAALVGGLRLVPSCNIGEGGIALAEAVPGTEPDFTEKNLANPTPLLLS 1133
+ +S+ CA L+GGL L PSCNIGEGGIALAEAV G+ PD KN+ANPT L+LS
Sbjct: 255 MPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALMLS 314
Query: 1134 TVMMLHHLKLHDKAD 1178
V ML HLKLHDKAD
Sbjct: 315 AVTMLRHLKLHDKAD 329
Score = 134 bits (336), Expect = 6e-29
Identities = 63/89 (70%), Positives = 77/89 (86%)
Frame = +3
Query: 3 PSCNIGEGNIALAEAVPGTTPEIDDMNLANPTPLLLSAVMMLQHLKLHDKADQIHDAIIK 182
PSCNIGEG IALAEAV G+ P+I N+ANPT L+LSAV ML+HLKLHDKADQI +AI+
Sbjct: 278 PSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALMLSAVTMLRHLKLHDKADQIQNAILN 337
Query: 183 TIAEGKYQTADIGGTSTTKDVTKSICNHL 269
TI+EG+Y+TAD+GG+STT D TK+IC+HL
Sbjct: 338 TISEGRYRTADLGGSSTTTDFTKAICDHL 366
>ref|XP_002519520.1| isocitrate dehydrogenase, putative [Ricinus communis]
gi|223541383|gb|EEF42934.1| isocitrate dehydrogenase,
putative [Ricinus communis]
Length = 364
Score = 331 bits (849), Expect = 2e-88
Identities = 174/307 (56%), Positives = 215/307 (70%), Gaps = 18/307 (5%)
Frame = +3
Query: 312 STSANADPSPSYPIQTTLFPGLGIGPDITESVKQVLSTAEVPIKWEEHFVGNKEDQRTKS 491
+ +A A S S PI TLFPG GIGP+I ESVKQV AEVPI+WEEH+VG++ D RT+S
Sbjct: 21 AAAARALFSTSTPITATLFPGDGIGPEIAESVKQVFKEAEVPIEWEEHYVGDQIDPRTQS 80
Query: 492 YLTWESLESIRKNGAGIKAPMWKPIGNAQRSIYHAFREELSLYASVKLFNSLYGYKPRKN 671
+LTWESLES+R+N G+K PM PIG RS+ R+EL+LYA+V+ SL GYK R +
Sbjct: 81 FLTWESLESVRRNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD 140
Query: 672 DVDIVTVT------------QQMRGVDES------QVCAHVAEYAFYYARTHGRDKVSAI 797
DV+++T+ Q +RGV ES Q VAEYAF+YA+THGR++VSAI
Sbjct: 141 DVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAI 200
Query: 798 HEPEITTKTDGLFLKCCREVANKYPEIRYEEITREACVIKLATDHAYFDVLAVDRFSDYS 977
H+ I KTDGLFLKCCREVA KYPEI+YEE+ + C + L + A FDVL +
Sbjct: 201 HKANIMQKTDGLFLKCCREVAEKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDI 260
Query: 978 VSNDCAALVGGLRLVPSCNIGEGGIALAEAVPGTEPDFTEKNLANPTPLLLSTVMMLHHL 1157
+S+ CA L+GGL L PSCNIGEGGIALAEAV G+ PD KNLANPT L+LS+V ML HL
Sbjct: 261 ISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALMLSSVTMLRHL 320
Query: 1158 KLHDKAD 1178
+LHDKAD
Sbjct: 321 ELHDKAD 327
Score = 129 bits (324), Expect = 1e-27
Identities = 61/89 (68%), Positives = 76/89 (85%)
Frame = +3
Query: 3 PSCNIGEGNIALAEAVPGTTPEIDDMNLANPTPLLLSAVMMLQHLKLHDKADQIHDAIIK 182
PSCNIGEG IALAEAV G+ P+I NLANPT L+LS+V ML+HL+LHDKAD+I AI+
Sbjct: 276 PSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALMLSSVTMLRHLELHDKADRIQSAILD 335
Query: 183 TIAEGKYQTADIGGTSTTKDVTKSICNHL 269
TI+EGKY+TAD+GG+S+T D TK+IC+HL
Sbjct: 336 TISEGKYRTADLGGSSSTTDFTKAICDHL 364
>ref|XP_003603020.1| Isocitrate dehydrogenase [Medicago truncatula]
gi|355492068|gb|AES73271.1| Isocitrate dehydrogenase
[Medicago truncatula]
Length = 353
Score = 329 bits (844), Expect = 7e-88
Identities = 174/316 (55%), Positives = 215/316 (68%), Gaps = 18/316 (5%)
Frame = +3
Query: 285 VSSPFITNISTSANADPSPSYPIQTTLFPGLGIGPDITESVKQVLSTAEVPIKWEEHFVG 464
++S + ++ + S + PI+ TLFPG GIGP+I ESVKQV AEVPI+WEEH+VG
Sbjct: 1 MASQILRRTFSNPRSFSSATTPIRATLFPGDGIGPEIAESVKQVFQAAEVPIEWEEHYVG 60
Query: 465 NKEDQRTKSYLTWESLESIRKNGAGIKAPMWKPIGNAQRSIYHAFREELSLYASVKLFNS 644
D RT+S+LTWESLES+RKN G+K PM PIG RS+ R+EL+LYA+V+ S
Sbjct: 61 TTIDPRTQSFLTWESLESVRKNRVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYS 120
Query: 645 LYGYKPRKNDVDIVTVT------------QQMRGVDES------QVCAHVAEYAFYYART 770
L GYK R ++VD++T+ Q +RGV ES Q VAEYAF+YA+
Sbjct: 121 LPGYKTRYDNVDLITIRENTEGEYSGLEHQVVRGVVESIKIITRQASLRVAEYAFHYAKA 180
Query: 771 HGRDKVSAIHEPEITTKTDGLFLKCCREVANKYPEIRYEEITREACVIKLATDHAYFDVL 950
HGR++VSAIH+ I KTDGLFLKCCREVANKYPEI YEE+ + C + L + A FDVL
Sbjct: 181 HGRERVSAIHKANIMQKTDGLFLKCCREVANKYPEIVYEEVVIDNCCMMLVKNPALFDVL 240
Query: 951 AVDRFSDYSVSNDCAALVGGLRLVPSCNIGEGGIALAEAVPGTEPDFTEKNLANPTPLLL 1130
+ +S+ CA L+GGL L PSCNIGEGGIALAEAV G+ PD KNLANPT LLL
Sbjct: 241 VMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLL 300
Query: 1131 STVMMLHHLKLHDKAD 1178
S+V ML HL LHDKAD
Sbjct: 301 SSVTMLRHLDLHDKAD 316
Score = 124 bits (310), Expect = 6e-26
Identities = 61/89 (68%), Positives = 73/89 (82%)
Frame = +3
Query: 3 PSCNIGEGNIALAEAVPGTTPEIDDMNLANPTPLLLSAVMMLQHLKLHDKADQIHDAIIK 182
PSCNIGEG IALAEAV G+ P+I NLANPT LLLS+V ML+HL LHDKAD I +AI+
Sbjct: 265 PSCNIGEGGIALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLDLHDKADGIQNAILN 324
Query: 183 TIAEGKYQTADIGGTSTTKDVTKSICNHL 269
TIAEGKY+TAD+GG+S T + TK+I +HL
Sbjct: 325 TIAEGKYRTADLGGSSKTTEFTKAIIDHL 353
>ref|XP_004143261.1| PREDICTED: isocitrate dehydrogenase [NAD] catalytic subunit 5,
mitochondrial-like [Cucumis sativus]
gi|449524456|ref|XP_004169239.1| PREDICTED: isocitrate
dehydrogenase [NAD] catalytic subunit 5,
mitochondrial-like [Cucumis sativus]
Length = 372
Score = 329 bits (843), Expect = 9e-88
Identities = 175/315 (55%), Positives = 218/315 (69%), Gaps = 18/315 (5%)
Frame = +3
Query: 288 SSPFITNISTSANADPSPSYPIQTTLFPGLGIGPDITESVKQVLSTAEVPIKWEEHFVGN 467
SSPF +SA + PI+ TLFPG GIGP+I ESVKQV TA+VPI+WEEH+VG+
Sbjct: 27 SSPFPFRAFSSA------ATPIRATLFPGDGIGPEIAESVKQVFRTADVPIEWEEHYVGD 80
Query: 468 KEDQRTKSYLTWESLESIRKNGAGIKAPMWKPIGNAQRSIYHAFREELSLYASVKLFNSL 647
+ D RT+S+LTWESLES+R+NG G+K PM PIG RS+ R+EL+LYA+V+ SL
Sbjct: 81 EIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSL 140
Query: 648 YGYKPRKNDVDIVTVT------------QQMRGVDES------QVCAHVAEYAFYYARTH 773
GYK R ++V+++T+ Q +RGV ES Q VAEYAF+YA+TH
Sbjct: 141 PGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTH 200
Query: 774 GRDKVSAIHEPEITTKTDGLFLKCCREVANKYPEIRYEEITREACVIKLATDHAYFDVLA 953
GR++VSAIH+ I KTDGLFLKCCREVA KYPEI+YEE+ + C + L + A FDVL
Sbjct: 201 GRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEVVIDNCCMMLVKNPALFDVLV 260
Query: 954 VDRFSDYSVSNDCAALVGGLRLVPSCNIGEGGIALAEAVPGTEPDFTEKNLANPTPLLLS 1133
+ +S+ CA L+GGL L PSCNIGEGGIALAEAV G+ PD KN+ANPT LLLS
Sbjct: 261 MPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALLLS 320
Query: 1134 TVMMLHHLKLHDKAD 1178
V ML HLKL +KAD
Sbjct: 321 AVTMLRHLKLQNKAD 335
Score = 127 bits (319), Expect = 5e-27
Identities = 61/89 (68%), Positives = 75/89 (84%)
Frame = +3
Query: 3 PSCNIGEGNIALAEAVPGTTPEIDDMNLANPTPLLLSAVMMLQHLKLHDKADQIHDAIIK 182
PSCNIGEG IALAEAV G+ P+I N+ANPT LLLSAV ML+HLKL +KAD+I DAI+
Sbjct: 284 PSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILN 343
Query: 183 TIAEGKYQTADIGGTSTTKDVTKSICNHL 269
TIAEGKY+TAD+GG+S+T + T +IC+HL
Sbjct: 344 TIAEGKYRTADLGGSSSTTEFTNAICDHL 372