BLASTX nr result
ID: Coptis24_contig00015984
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00015984
(1249 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002284968.1| PREDICTED: gibberellin 20 oxidase 3 [Vitis v... 511 e-142
emb|CAN80138.1| hypothetical protein VITISV_028512 [Vitis vinifera] 509 e-142
ref|XP_002517541.1| Gibberellin 20 oxidase, putative [Ricinus co... 481 e-133
ref|XP_002298129.1| 2-oxoglutarate-dependent dioxygenase [Populu... 477 e-132
ref|XP_003528366.1| PREDICTED: gibberellin 20 oxidase 1-like [Gl... 454 e-125
>ref|XP_002284968.1| PREDICTED: gibberellin 20 oxidase 3 [Vitis vinifera]
gi|297743335|emb|CBI36202.3| unnamed protein product
[Vitis vinifera]
Length = 314
Score = 511 bits (1316), Expect = e-142
Identities = 234/314 (74%), Positives = 280/314 (89%)
Frame = +2
Query: 194 MGSESPLRLPTIDFSNVDLKPGTIVWNSVKLQVKEALEEFGCFEAVYNQVPAELHKALFS 373
MGSE+PL+LP IDFS+ +LKPGT+ WN VK QV++ALEE+GCFEA +N+VP +L KA+F
Sbjct: 1 MGSETPLKLPVIDFSDPELKPGTLQWNLVKAQVQKALEEYGCFEAFFNKVPLDLRKAIFG 60
Query: 374 SLEELFDLPLRTKLQNRSSKPFHGYVGQYPMIPLYESMGIEDAPTLEIAENFTNMMWPEG 553
+LEELFDLPL+TKL+N S KP+HGYVGQYP +PLYESMGI+DA E AE+FT ++WPEG
Sbjct: 61 ALEELFDLPLQTKLRNSSKKPYHGYVGQYPTVPLYESMGIDDALVQEKAEHFTKLLWPEG 120
Query: 554 NANFCKTVYSYSKQVAELDQVVRRMVLESLGVEKYYDEHVESTTYLLRVMKYKGPQTNET 733
N NFCKT+ S+S+Q++ELD +RRMVLESLG+EKY DEH++ST YLLRVMKYKGPQT ET
Sbjct: 121 NPNFCKTIQSFSEQLSELDHTIRRMVLESLGMEKYLDEHMDSTNYLLRVMKYKGPQTTET 180
Query: 734 KLGLNSHTDKNIVTILSQNQVHGLEVQTKDGEWIDVKVSPNSFVVVIGDSFLAWTNGRLH 913
KLGLN+HTDKNIVTIL QNQV GL VQTKDG+WI++K SP+SF+++IGDSFLAWTNGRLH
Sbjct: 181 KLGLNAHTDKNIVTILYQNQVDGLGVQTKDGQWINIKCSPDSFIIMIGDSFLAWTNGRLH 240
Query: 914 SPHHRVMMTGNEARYSTGLFSFPKAGYIVKAPEELVDEDHPLLFKPFEHVEFFSFYYTEE 1093
SP+HRVMMTGNE+RYSTGLFS PKAGY++KAPEELVDE+HPLLFKPF+H+EF FYYTE
Sbjct: 241 SPYHRVMMTGNESRYSTGLFSIPKAGYVIKAPEELVDEEHPLLFKPFDHLEFLQFYYTEA 300
Query: 1094 GQMCKSALKTYCGV 1135
GQ +SALKTYCGV
Sbjct: 301 GQRAESALKTYCGV 314
>emb|CAN80138.1| hypothetical protein VITISV_028512 [Vitis vinifera]
Length = 314
Score = 509 bits (1312), Expect = e-142
Identities = 234/314 (74%), Positives = 279/314 (88%)
Frame = +2
Query: 194 MGSESPLRLPTIDFSNVDLKPGTIVWNSVKLQVKEALEEFGCFEAVYNQVPAELHKALFS 373
MGSE+PL+LP IDFS+ +LKPGT+ WN VK QV++ALEE+GCFEA +N+VP +L KA+F
Sbjct: 1 MGSETPLKLPXIDFSDPELKPGTLQWNLVKAQVQKALEEYGCFEAFFNKVPLDLRKAIFG 60
Query: 374 SLEELFDLPLRTKLQNRSSKPFHGYVGQYPMIPLYESMGIEDAPTLEIAENFTNMMWPEG 553
+LEELFDLPL+TKL+N S KP+HGYVGQYP +PLYESMGI+DA E AE+FT ++WPEG
Sbjct: 61 ALEELFDLPLQTKLRNSSKKPYHGYVGQYPTVPLYESMGIDDALVQEKAEHFTKLLWPEG 120
Query: 554 NANFCKTVYSYSKQVAELDQVVRRMVLESLGVEKYYDEHVESTTYLLRVMKYKGPQTNET 733
N NFCKT+ S+S+Q++ELD +RRMVLESLG+EKY DEH++ST YLLRVMKYKGPQT ET
Sbjct: 121 NPNFCKTIQSFSEQLSELDHTIRRMVLESLGMEKYLDEHMDSTNYLLRVMKYKGPQTTET 180
Query: 734 KLGLNSHTDKNIVTILSQNQVHGLEVQTKDGEWIDVKVSPNSFVVVIGDSFLAWTNGRLH 913
KLGLN+HTDKNIVTIL QNQV GL VQTKDG WI++K SP+SF+++IGDSFLAWTNGRLH
Sbjct: 181 KLGLNAHTDKNIVTILYQNQVDGLGVQTKDGXWINIKCSPDSFIIMIGDSFLAWTNGRLH 240
Query: 914 SPHHRVMMTGNEARYSTGLFSFPKAGYIVKAPEELVDEDHPLLFKPFEHVEFFSFYYTEE 1093
SP+HRVMMTGNE+RYSTGLFS PKAGY++KAPEELVDE+HPLLFKPF+H+EF FYYTE
Sbjct: 241 SPYHRVMMTGNESRYSTGLFSIPKAGYVIKAPEELVDEEHPLLFKPFDHLEFLQFYYTEA 300
Query: 1094 GQMCKSALKTYCGV 1135
GQ +SALKTYCGV
Sbjct: 301 GQRAESALKTYCGV 314
>ref|XP_002517541.1| Gibberellin 20 oxidase, putative [Ricinus communis]
gi|223543173|gb|EEF44705.1| Gibberellin 20 oxidase,
putative [Ricinus communis]
Length = 314
Score = 481 bits (1239), Expect = e-133
Identities = 227/314 (72%), Positives = 270/314 (85%)
Frame = +2
Query: 194 MGSESPLRLPTIDFSNVDLKPGTIVWNSVKLQVKEALEEFGCFEAVYNQVPAELHKALFS 373
MGSE+PL+LP IDFS +LKPGT+ W VK QV+ ALEE+GCFEA +++V +L K +F
Sbjct: 1 MGSETPLKLPAIDFSTENLKPGTVEWELVKTQVRNALEEYGCFEAFFSRVHNDLKKGVFG 60
Query: 374 SLEELFDLPLRTKLQNRSSKPFHGYVGQYPMIPLYESMGIEDAPTLEIAENFTNMMWPEG 553
++EELF+LPL+TKL+N S KPFHGYVGQYP PLYESMGIED T+E E+FT+++WP+G
Sbjct: 61 AIEELFELPLQTKLKNVSRKPFHGYVGQYPQAPLYESMGIEDPDTVEKVESFTSVLWPQG 120
Query: 554 NANFCKTVYSYSKQVAELDQVVRRMVLESLGVEKYYDEHVESTTYLLRVMKYKGPQTNET 733
N F KTV S+S+QV++LD+VVRRM++ESLG+EKY DEH+ ST YLLRVMKYKGP+T+E
Sbjct: 121 NLTFSKTVQSFSEQVSKLDKVVRRMIVESLGLEKYMDEHMNSTDYLLRVMKYKGPETSEA 180
Query: 734 KLGLNSHTDKNIVTILSQNQVHGLEVQTKDGEWIDVKVSPNSFVVVIGDSFLAWTNGRLH 913
KLGL+ HTDKNIVTIL QNQV GLEV+TK+GEWI VK+SP+SFVV+IGDS LAW NGRLH
Sbjct: 181 KLGLHPHTDKNIVTILFQNQVDGLEVKTKNGEWITVKLSPDSFVVMIGDSLLAWANGRLH 240
Query: 914 SPHHRVMMTGNEARYSTGLFSFPKAGYIVKAPEELVDEDHPLLFKPFEHVEFFSFYYTEE 1093
SP+HRVMMTGNEARYS GLFS PKAGYIVKAPEELVDE+HPLLFKPF+HVEF FYYTE
Sbjct: 241 SPYHRVMMTGNEARYSCGLFSIPKAGYIVKAPEELVDEEHPLLFKPFDHVEFLGFYYTEA 300
Query: 1094 GQMCKSALKTYCGV 1135
GQ +SALK YCGV
Sbjct: 301 GQRAQSALKAYCGV 314
>ref|XP_002298129.1| 2-oxoglutarate-dependent dioxygenase [Populus trichocarpa]
gi|222845387|gb|EEE82934.1| 2-oxoglutarate-dependent
dioxygenase [Populus trichocarpa]
Length = 314
Score = 477 bits (1228), Expect = e-132
Identities = 220/314 (70%), Positives = 269/314 (85%)
Frame = +2
Query: 194 MGSESPLRLPTIDFSNVDLKPGTIVWNSVKLQVKEALEEFGCFEAVYNQVPAELHKALFS 373
MGSE+PL+LP IDFSN+ PG W+ VKLQV++ALEE+GCFEA+++++PAE KA+F
Sbjct: 1 MGSETPLKLPIIDFSNLGQNPGAAEWDLVKLQVRKALEEYGCFEALFDKIPAESRKAIFG 60
Query: 374 SLEELFDLPLRTKLQNRSSKPFHGYVGQYPMIPLYESMGIEDAPTLEIAENFTNMMWPEG 553
++EELFDLPL+TK++N S KP+HGYVGQYP +PL+ESMGI+DA E E+ T ++WP+G
Sbjct: 61 AVEELFDLPLQTKMRNASKKPYHGYVGQYPQVPLFESMGIDDANIAEEVESMTTILWPQG 120
Query: 554 NANFCKTVYSYSKQVAELDQVVRRMVLESLGVEKYYDEHVESTTYLLRVMKYKGPQTNET 733
N +F TV S+S+QV+ELDQ+VRRM++ESLG+EKY DEH+ ST YLLRVMKYKGPQT ET
Sbjct: 121 NQSFSNTVLSFSEQVSELDQIVRRMIVESLGLEKYLDEHMNSTNYLLRVMKYKGPQTTET 180
Query: 734 KLGLNSHTDKNIVTILSQNQVHGLEVQTKDGEWIDVKVSPNSFVVVIGDSFLAWTNGRLH 913
KLGL +HTDKN+VTIL QNQV GLE+QTKDG WID+K +P+SF+V+IGDS AW NGRLH
Sbjct: 181 KLGLTAHTDKNMVTILYQNQVDGLELQTKDGCWIDLKPTPDSFIVMIGDSLHAWANGRLH 240
Query: 914 SPHHRVMMTGNEARYSTGLFSFPKAGYIVKAPEELVDEDHPLLFKPFEHVEFFSFYYTEE 1093
SP+HRVMM GNEARYS GLFS PKAGYIVKAPEEL+DE+HPLLFKPF+HV+F FYYTE
Sbjct: 241 SPYHRVMMRGNEARYSVGLFSVPKAGYIVKAPEELIDEEHPLLFKPFDHVKFLGFYYTEA 300
Query: 1094 GQMCKSALKTYCGV 1135
GQ +SALKTYCGV
Sbjct: 301 GQRAQSALKTYCGV 314
>ref|XP_003528366.1| PREDICTED: gibberellin 20 oxidase 1-like [Glycine max]
Length = 314
Score = 454 bits (1168), Expect = e-125
Identities = 208/314 (66%), Positives = 264/314 (84%)
Frame = +2
Query: 194 MGSESPLRLPTIDFSNVDLKPGTIVWNSVKLQVKEALEEFGCFEAVYNQVPAELHKALFS 373
MGSE+ L+LP IDF+N+ L+ W ++K QV +AL ++GCFEA++ +VP EL KA+F+
Sbjct: 1 MGSEATLKLPVIDFTNLKLEANNPNWEAIKSQVHKALVDYGCFEAIFEKVPLELRKAIFA 60
Query: 374 SLEELFDLPLRTKLQNRSSKPFHGYVGQYPMIPLYESMGIEDAPTLEIAENFTNMMWPEG 553
+L+ELFDLPL+TK+ N S KP+HGYVGQYPM+PL+ESMGI+DA E E+ TN+MWP G
Sbjct: 61 ALQELFDLPLQTKILNVSKKPYHGYVGQYPMVPLFESMGIDDANVYENVESMTNIMWPHG 120
Query: 554 NANFCKTVYSYSKQVAELDQVVRRMVLESLGVEKYYDEHVESTTYLLRVMKYKGPQTNET 733
N +F KT+ S+S+Q++ELDQ++R+M+LESLGVEKY +EH+ ST YLLRVMKYKGPQT++T
Sbjct: 121 NPSFSKTIQSFSEQLSELDQIIRKMILESLGVEKYLEEHMNSTNYLLRVMKYKGPQTSDT 180
Query: 734 KLGLNSHTDKNIVTILSQNQVHGLEVQTKDGEWIDVKVSPNSFVVVIGDSFLAWTNGRLH 913
K+GL +H+DKNIVTIL QN+V GLEV TKDG+WI + SP+SFVV+IGDS AW+NGRLH
Sbjct: 181 KVGLTTHSDKNIVTILYQNEVEGLEVMTKDGKWISYRPSPDSFVVMIGDSLHAWSNGRLH 240
Query: 914 SPHHRVMMTGNEARYSTGLFSFPKAGYIVKAPEELVDEDHPLLFKPFEHVEFFSFYYTEE 1093
SP HRVMM+GNEARYS GLFS PK G I+KAPEELVDE+HPLLFKPF+HVEF +YYTE+
Sbjct: 241 SPFHRVMMSGNEARYSAGLFSIPKGGNIIKAPEELVDEEHPLLFKPFDHVEFLKYYYTEK 300
Query: 1094 GQMCKSALKTYCGV 1135
GQ + AL+TYCGV
Sbjct: 301 GQRDQFALRTYCGV 314