BLASTX nr result
ID: Coptis24_contig00012988
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00012988
(1954 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003538157.1| PREDICTED: 4-coumarate--CoA ligase-like 7-li... 425 e-116
ref|XP_002893114.1| opc-8:0 CoA ligase1 [Arabidopsis lyrata subs... 422 e-115
gb|AAP03021.1| 4-coumarate-CoA ligase-like protein [Arabidopsis ... 422 e-115
ref|NP_564115.1| OPC-8:0 CoA ligase1 [Arabidopsis thaliana] gi|1... 422 e-115
gb|ABR17473.1| unknown [Picea sitchensis] 419 e-114
>ref|XP_003538157.1| PREDICTED: 4-coumarate--CoA ligase-like 7-like [Glycine max]
Length = 548
Score = 425 bits (1093), Expect = e-116
Identities = 234/538 (43%), Positives = 338/538 (62%), Gaps = 19/538 (3%)
Frame = -3
Query: 1763 GLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITYGELRSLTSSLA 1584
G+Y S +LP P+LSL SFL +SS K A +DS S +++T L+S + LA
Sbjct: 10 GIYRSLRPCLVLPNDPNLSLVSFLFQSVSSFP-SKIALVDSHSSQTLTLAHLKSQVAKLA 68
Query: 1583 HVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPELQSQIQDTNPN 1404
H F LGI K D+VL++ PNS++YP+ L ++GA+ STANP++T E+ Q+ D+NP
Sbjct: 69 HGFLKLGINKNDVVLLLAPNSIHYPICFLAATAIGAVVSTANPIYTVNEISKQVDDSNPK 128
Query: 1403 LIITTPEQRSKLD---------DRQSTRSIILVD--------DLMDTLLQYPRHHKTLGT 1275
L+IT PE K+ D ++ + ++ + +D +++ L
Sbjct: 129 LLITVPELWDKVKNLNLPAVIIDTETAQGLVSFEAGNEVSRITSLDAVMEMAGPATELPE 188
Query: 1274 E-INQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCVLPLF 1098
+ Q D AAL+YSSGTTG SK VV HRNFIA S + + + G DD+YLCVLP+F
Sbjct: 189 SGVKQGDTAALLYSSGTTGLSKGVVLTHRNFIAASVMIG-MDDDLAGEQDDVYLCVLPMF 247
Query: 1097 HMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXQDGVK 918
H+FGL++ L S VV+ ++ LE +L ++EK +VT V
Sbjct: 248 HVFGLAVVTYAALRRGSAVVVMERFELEALLKAIEKQRVTKLWVVPPILLGLAKQ-SVVG 306
Query: 917 GYDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSGMVHE- 741
YDL S++ + APLGK ME+ + +P + + Q YG+TETCG +++ N G+ H
Sbjct: 307 NYDLSSLRRIGSGAAPLGKDLMEECGRRFPHVAICQGYGMTETCGIVSVENPRVGVRHTG 366
Query: 740 SIGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDPNATSLAINKEGWLHT 561
S G L+ +EA+IV V T K LPP + GE+ +RGP++MQGY +P AT L I+K+GW+HT
Sbjct: 367 STGTLVSGVEAQIVSVDTQKPLPPRQLGEIWVRGPNMMQGYHNNPEATRLTIDKKGWVHT 426
Query: 560 GDLCFIDKLGLVYIVDRIKELIKYKAYQVAPAELEEILLIHPDINEVAVIPYPDEAAGEI 381
GDL + D+ G +Y+VDRIKELIKYK +QVAPAELE +L+ HP+I E V+PYPD+ AGE+
Sbjct: 427 GDLGYFDEDGQLYVVDRIKELIKYKGFQVAPAELEGLLVSHPEILEAVVVPYPDDEAGEV 486
Query: 380 PMACVVKRGMSEVEEEDIISFVATKVAPYKKIRKVVFVDSIPRSASGKILRRHLRAKA 207
P+A VV+ S + EE+I F+A +VAP+KK+R+V F++++P++ASGKILRR L AKA
Sbjct: 487 PIAYVVRSPNSSLTEEEIQKFIAKQVAPFKKLRRVTFINNVPKTASGKILRRELTAKA 544
>ref|XP_002893114.1| opc-8:0 CoA ligase1 [Arabidopsis lyrata subsp. lyrata]
gi|297338956|gb|EFH69373.1| opc-8:0 CoA ligase1
[Arabidopsis lyrata subsp. lyrata]
Length = 546
Score = 422 bits (1085), Expect = e-115
Identities = 232/543 (42%), Positives = 339/543 (62%), Gaps = 17/543 (3%)
Frame = -3
Query: 1796 VSIDSGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITY 1617
V+ SGFC+S + SK LP P L + +F+ SQ + AFID+ + +++T+
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRI---AFIDASTGQNLTF 60
Query: 1616 GELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPE 1437
EL S++ +GI+KG +VL++ PNS+ +P++ L VMSLGAI +T NPL+T E
Sbjct: 61 SELWRAVESVSDCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 120
Query: 1436 LQSQIQDTNPNLIITTPEQRSKLDDRQSTRSIILVDD-----------LMDTLLQYPRHH 1290
+ QI+D+NP L TT + K+ I+L+D+ L++ + + P +
Sbjct: 121 IAKQIKDSNPVLAFTTSQLLPKIAAAAKKLPIVLMDEERVDSVGKVRRLVEMMKKEPSGN 180
Query: 1289 KTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 1110
+ + ++QDD A L+YSSGTTG SK V+ HRN IAM + + + S+DG + ++C
Sbjct: 181 RVM-ERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDG--EQRFICT 237
Query: 1109 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXQ 930
+P+FH++GL+ F G LA ST +VL+K+ + +M+ ++ KY+ T
Sbjct: 238 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 297
Query: 929 DGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSG 753
D +K YDL S+ V+C GAPL K E F++ YP +++ Q YGLTE+ G +
Sbjct: 298 DQIKAKYDLSSLHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG----IGASTD 353
Query: 752 MVHES-----IGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDPNATSLA 588
V ES G L SME +IVD TG+ L P + GEL L+GPS+M+GYF + ATS
Sbjct: 354 TVEESRRYGTAGKLSASMEGRIVDPVTGQILGPNQTGELWLKGPSIMKGYFSNEEATSST 413
Query: 587 INKEGWLHTGDLCFIDKLGLVYIVDRIKELIKYKAYQVAPAELEEILLIHPDINEVAVIP 408
++ EGWL TGDLC+ID+ G +++VDR+KELIKYK YQVAPAELE +LL HP+I + AVIP
Sbjct: 414 LDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIP 473
Query: 407 YPDEAAGEIPMACVVKRGMSEVEEEDIISFVATKVAPYKKIRKVVFVDSIPRSASGKILR 228
+PD+ G+ PMA VV++ S + E+ I+ FVA +VAPYK+IRKV FV SIP++ SGKILR
Sbjct: 474 FPDKEVGQFPMAYVVRKTGSSLSEKSIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILR 533
Query: 227 RHL 219
+ L
Sbjct: 534 KDL 536
>gb|AAP03021.1| 4-coumarate-CoA ligase-like protein [Arabidopsis thaliana]
Length = 546
Score = 422 bits (1085), Expect = e-115
Identities = 233/543 (42%), Positives = 339/543 (62%), Gaps = 17/543 (3%)
Frame = -3
Query: 1796 VSIDSGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITY 1617
V+ SGFC+S + SK LP P L + +F+ SQ + AFID+ + +++T+
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRI---AFIDASTGQNLTF 60
Query: 1616 GELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPE 1437
EL S+A +GI+KG +VL++ PNS+ +P++ L VMSLGAI +T NPL+T E
Sbjct: 61 TELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 120
Query: 1436 LQSQIQDTNPNLIITTPEQRSKLDDRQSTRSIILVDD-----------LMDTLLQYPRHH 1290
+ QI+D+NP L TT + K+ I+L+D+ L++ + + P +
Sbjct: 121 IAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGN 180
Query: 1289 KTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 1110
+ + ++QDD A L+YSSGTTG SK V+ HRN IAM + + + S+DG + ++C
Sbjct: 181 R-VKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDG--EQRFICT 237
Query: 1109 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXQ 930
+P+FH++GL+ F G LA ST +VL+K+ + +M+ ++ KY+ T
Sbjct: 238 VPMFHIYGLTAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 297
Query: 929 DGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSG 753
D +K YDL S+ V+C GAPL K E F++ YP +++ Q YGLTE+ G +
Sbjct: 298 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG----IGASTD 353
Query: 752 MVHES-----IGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDPNATSLA 588
V ES G L SME +IVD TG+ L P + GEL L+GPS+M+GYF + ATS
Sbjct: 354 TVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSST 413
Query: 587 INKEGWLHTGDLCFIDKLGLVYIVDRIKELIKYKAYQVAPAELEEILLIHPDINEVAVIP 408
++ EGWL TGDLC+ID+ G +++VDR+KELIKYK YQVAPAELE +LL HP+I + AVIP
Sbjct: 414 LDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIP 473
Query: 407 YPDEAAGEIPMACVVKRGMSEVEEEDIISFVATKVAPYKKIRKVVFVDSIPRSASGKILR 228
+PD+ G+ PMA VV++ S + E+ I+ FVA +VAPYK+IRKV FV SIP++ SGKILR
Sbjct: 474 FPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILR 533
Query: 227 RHL 219
+ L
Sbjct: 534 KDL 536
>ref|NP_564115.1| OPC-8:0 CoA ligase1 [Arabidopsis thaliana]
gi|158564046|sp|Q84P21.2|4CLL5_ARATH RecName:
Full=4-coumarate--CoA ligase-like 5; AltName:
Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9;
AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1
gi|13430676|gb|AAK25960.1|AF360250_1 unknown protein
[Arabidopsis thaliana] gi|14532846|gb|AAK64105.1| unknown
protein [Arabidopsis thaliana]
gi|116490123|gb|ABJ98946.1| peroxisomal OPC-8:0 CoA
ligase [Arabidopsis thaliana] gi|332191859|gb|AEE29980.1|
OPC-8:0 CoA ligase1 [Arabidopsis thaliana]
Length = 546
Score = 422 bits (1085), Expect = e-115
Identities = 233/543 (42%), Positives = 339/543 (62%), Gaps = 17/543 (3%)
Frame = -3
Query: 1796 VSIDSGFCSSTGLYCSKWGSPLLPQTPHLSLPSFLLSQISSKALDKPAFIDSKSCKSITY 1617
V+ SGFC+S + SK LP P L + +F+ SQ + AFID+ + +++T+
Sbjct: 4 VNSRSGFCNSNSTFYSKRTPIPLPPNPSLDVTTFISSQAHRGRI---AFIDASTGQNLTF 60
Query: 1616 GELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLHTRPE 1437
EL S+A +GI+KG +VL++ PNS+ +P++ L VMSLGAI +T NPL+T E
Sbjct: 61 TELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNE 120
Query: 1436 LQSQIQDTNPNLIITTPEQRSKLDDRQSTRSIILVDD-----------LMDTLLQYPRHH 1290
+ QI+D+NP L TT + K+ I+L+D+ L++ + + P +
Sbjct: 121 IAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGN 180
Query: 1289 KTLGTEINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 1110
+ + ++QDD A L+YSSGTTG SK V+ HRN IAM + + + S+DG + ++C
Sbjct: 181 R-VKERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDG--EQRFICT 237
Query: 1109 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXQ 930
+P+FH++GL+ F G LA ST +VL+K+ + +M+ ++ KY+ T
Sbjct: 238 VPMFHIYGLAAFATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGA 297
Query: 929 DGVKG-YDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSG 753
D +K YDL S+ V+C GAPL K E F++ YP +++ Q YGLTE+ G +
Sbjct: 298 DQIKAKYDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTG----IGASTD 353
Query: 752 MVHES-----IGGLIPSMEAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDPNATSLA 588
V ES G L SME +IVD TG+ L P + GEL L+GPS+M+GYF + ATS
Sbjct: 354 TVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSST 413
Query: 587 INKEGWLHTGDLCFIDKLGLVYIVDRIKELIKYKAYQVAPAELEEILLIHPDINEVAVIP 408
++ EGWL TGDLC+ID+ G +++VDR+KELIKYK YQVAPAELE +LL HP+I + AVIP
Sbjct: 414 LDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIP 473
Query: 407 YPDEAAGEIPMACVVKRGMSEVEEEDIISFVATKVAPYKKIRKVVFVDSIPRSASGKILR 228
+PD+ G+ PMA VV++ S + E+ I+ FVA +VAPYK+IRKV FV SIP++ SGKILR
Sbjct: 474 FPDKEVGQFPMAYVVRKTGSSLSEKTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILR 533
Query: 227 RHL 219
+ L
Sbjct: 534 KDL 536
>gb|ABR17473.1| unknown [Picea sitchensis]
Length = 548
Score = 419 bits (1078), Expect = e-114
Identities = 240/547 (43%), Positives = 338/547 (61%), Gaps = 9/547 (1%)
Frame = -3
Query: 1805 EMEVSIDSGFCSSTGLYCSKWGSPLLPQTPH-LSLPSFLLSQISSKALDKPAFIDSKSCK 1629
+ +V SGFC + G+Y SK LLP H L + +++ S + + + AFID S
Sbjct: 10 DFQVDQQSGFCKANGIYYSKREPVLLPPPHHPLDVITYIFSLQHN--MQQIAFIDGPSGT 67
Query: 1628 SITYGELRSLTSSLAHVFHSLGIKKGDIVLVVVPNSLYYPLLVLGVMSLGAIFSTANPLH 1449
++TY LR +LA H LGI+KGD+VLV+ NS+ P + L ++S+GAI +TANPL
Sbjct: 68 TLTYSALRLKVRALAAGLHGLGIRKGDVVLVISSNSIALPCIHLAILSIGAILTTANPLS 127
Query: 1448 TRPELQSQIQDTNPNLIITTPEQRSKLDDRQSTRSIILVDDLMDTLLQYPRHHKTLGT-- 1275
T E+ +Q++D+ P + T KL R + IIL+ +++ H
Sbjct: 128 TEREIVTQVKDSKPVIAFTLSHLIHKL--RATQIPIILIQGTLESRCVTTLHDLLQSDLK 185
Query: 1274 -----EINQDDIAALMYSSGTTGKSKSVVCLHRNFIAMSCFLRHVWESEDGAFDDIYLCV 1110
+I QDD A L+YSSGTTGKSK VV H N+IA R +E+E +Y+C
Sbjct: 186 DMPSIDIKQDDTATLLYSSGTTGKSKGVVSTHGNYIATIAGNRVRYETEGCK---MYICS 242
Query: 1109 LPLFHMFGLSMFVCGTLAVQSTTVVLNKYSLEDMLVSVEKYKVTXXXXXXXXXXXXXXXQ 930
+PLFH++GL VC TLA +T VV K+ +E++L S+E+Y+VT
Sbjct: 243 MPLFHIYGLRFLVC-TLAAGATIVVPPKFDMEEILRSIERYRVTLLPTVPSVLAALAKST 301
Query: 929 DGVKGYDLGSVKEVICSGAPLGKVHMEKFSKCYPQMRLSQCYGLTETCGPITLCNGFSGM 750
G + YDLGS++++ GAPLGK F+ +P++++ Q YGLTET G I N
Sbjct: 302 -GAQKYDLGSLQQISLGGAPLGKDVTLTFNAKFPRIQIRQGYGLTETTGAIAYTNSDEEN 360
Query: 749 VHESIGGLIPSM-EAKIVDVRTGKSLPPLECGELCLRGPSVMQGYFGDPNATSLAINKEG 573
GL+ + EAK+VD + K LPP + GEL LRGP+VM+GYFG+ +AT+ ++ EG
Sbjct: 361 RRNGTVGLLSDVVEAKVVDPDSAKPLPPNQRGELWLRGPTVMKGYFGNEHATATTLDSEG 420
Query: 572 WLHTGDLCFIDKLGLVYIVDRIKELIKYKAYQVAPAELEEILLIHPDINEVAVIPYPDEA 393
WL TGDLC+ D+ G +++VDR+K+LIKYKAYQVAPAELEE+LL HP+I+E AVIPYPD+
Sbjct: 421 WLRTGDLCYFDEEGFLFVVDRLKDLIKYKAYQVAPAELEELLLSHPEISEAAVIPYPDQE 480
Query: 392 AGEIPMACVVKRGMSEVEEEDIISFVATKVAPYKKIRKVVFVDSIPRSASGKILRRHLRA 213
AG+IPMA V + S + E D+I FVA +V YKKIR+V F +SIP++ASGKILR+ L
Sbjct: 481 AGQIPMAFVARSPSSNLSESDVIKFVAEQVVHYKKIRRVAFTNSIPKNASGKILRKDLIE 540
Query: 212 KALTTQQ 192
K + T +
Sbjct: 541 KNVPTSR 547