BLASTX nr result
ID: Coptis24_contig00011773
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00011773
(1545 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002278009.1| PREDICTED: cytochrome P450 94A1 [Vitis vinif... 420 0.0
ref|XP_004136768.1| PREDICTED: cytochrome P450 94A1-like [Cucumi... 422 0.0
ref|XP_002519477.1| cytochrome P450, putative [Ricinus communis]... 400 0.0
ref|XP_004167155.1| PREDICTED: cytochrome P450 94A1-like [Cucumi... 419 0.0
ref|XP_003521105.1| PREDICTED: cytochrome P450 94A1-like [Glycin... 402 e-176
>ref|XP_002278009.1| PREDICTED: cytochrome P450 94A1 [Vitis vinifera]
Length = 500
Score = 420 bits (1080), Expect(2) = 0.0
Identities = 203/310 (65%), Positives = 252/310 (81%)
Frame = -2
Query: 1544 FSISLYFFFHNSKPSKTKPISEDNFESYPYVGNLPVFIKNRHRFLDWTTNILRNTPTNTI 1365
F +SLYF+ SKTKPIS F++YP +G++P F+KNRHRFLDWTT++L PTNT
Sbjct: 13 FLLSLYFY-----SSKTKPIST-GFKAYPLLGSIPEFLKNRHRFLDWTTDVLSRCPTNTS 66
Query: 1364 TFRRPGKVQGVITANPLNVEHMLKTNFENYPKGNRFIFLLKDFLGRGIFNSDNELWRIQR 1185
T RRPG + GVITANPLNVEHMLKTNFENYPKG +FI +L+DFLGRGIFNSD +LW++QR
Sbjct: 67 TIRRPGGIHGVITANPLNVEHMLKTNFENYPKGPQFISILEDFLGRGIFNSDADLWKVQR 126
Query: 1184 KVASFEFSTRSLRNFVIENVRLELQTRLIPLLSNASKTKKTINIQDVLERFAFDNVCKVA 1005
K AS+EF+T+SLRNFV E+V +E QTRL+P+L A++T + +++QD+LERFAFDNVCK+A
Sbjct: 127 KTASYEFNTKSLRNFVFESVTVETQTRLVPILKKAAETGRVLDVQDILERFAFDNVCKLA 186
Query: 1004 FNEDPACLEGDGTSGSEFMSAFEDAATLSAGRFMYIFPHVWIFKRFFNIGSEKRLRESIK 825
FN DPACL GD T+G +FM AFEDAATLS+GRFMY F +W ++FFN+ EKRL++SI
Sbjct: 187 FNVDPACLGGDATAGGDFMRAFEDAATLSSGRFMYAFQSIWKMEKFFNMRKEKRLKKSIA 246
Query: 824 IVHEFAENIIKTRLQDKGIKQEADLLSRFIAANDENAPNSLEFLRDIVISFILAGRDTTS 645
IVHEFAE II++RL+ K ++ DLLSRF+ N NS EFLRDIVISFILAGRDTTS
Sbjct: 247 IVHEFAEKIIQSRLEQKAENKDQDLLSRFVG----NYDNSPEFLRDIVISFILAGRDTTS 302
Query: 644 TALTWFFWFI 615
+ALTWFFW +
Sbjct: 303 SALTWFFWLL 312
Score = 281 bits (719), Expect(2) = 0.0
Identities = 128/183 (69%), Positives = 153/183 (83%)
Frame = -3
Query: 607 PHIERNILQELKTIRNDSGKCIGETYELDEFRQMNYLHAAISEAMRLYPPVAVDTKACLA 428
P +E NIL+EL+T+R +GK IG+TY DE R+++YLHAAISE MRLYPPV VDTK CL
Sbjct: 316 PEVETNILKELETVRARNGKSIGDTYNFDELRELHYLHAAISETMRLYPPVPVDTKYCLN 375
Query: 427 DDMLPDGTFIGKGWFATYQAYAMGRMENIWGKDCNEFVPERWLENGIFQPENPFRFPIFH 248
DD+LPDGTF+GKGWF TY Y+MGR+E+IWGKDC +F PERWLENG+++ ENPFR+PIFH
Sbjct: 376 DDVLPDGTFVGKGWFMTYIPYSMGRIESIWGKDCLQFKPERWLENGVYRQENPFRYPIFH 435
Query: 247 GGARMCLGKEMAYIQMKSIAASVIENFEINAVDKDTCPQHVLSLTLRMKDGLMVRVKERS 68
G RMCLGK+MAYIQMKSIAASV+E FE+ +KD CP+H LSLTLRM GL VRVKER
Sbjct: 436 AGPRMCLGKDMAYIQMKSIAASVMEQFEVKVEEKDKCPEHYLSLTLRMTGGLEVRVKERC 495
Query: 67 MDV 59
+DV
Sbjct: 496 VDV 498
>ref|XP_004136768.1| PREDICTED: cytochrome P450 94A1-like [Cucumis sativus]
Length = 511
Score = 422 bits (1085), Expect(2) = 0.0
Identities = 208/313 (66%), Positives = 256/313 (81%), Gaps = 3/313 (0%)
Frame = -2
Query: 1544 FSISLYFFFHNSKPSKTKPISEDNFESYPYVGNLPVFIKNRHRFLDWTTNILRNTPTNTI 1365
FS S+YF F S SK+KP + F+ YP+VG LP+F+ NRHRFLDW+T +LRN TNT
Sbjct: 17 FSFSIYFLFFPSTKSKSKP--QQGFKHYPFVGTLPLFLLNRHRFLDWSTEVLRNCRTNTS 74
Query: 1364 TFRRPGKVQGVITANPLNVEHMLKTNFENYPKGNRFIFLLKDFLGRGIFNSDNELWRIQR 1185
F+RPGKV GVITANPL VEH+LKT FENYPKG RFI LL+DFLGRGIFNSD E+W++QR
Sbjct: 75 VFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNSDGEIWKVQR 134
Query: 1184 KVASFEFSTRSLRNFVIENVRLELQTRLIPLLSNASKTKKTINIQDVLERFAFDNVCKVA 1005
K AS+EF+T+SLRNFV+ENVR+E+Q+RL+P+ A +T++ +++QDVLERFAFDNVCK+A
Sbjct: 135 KTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQDVLERFAFDNVCKLA 194
Query: 1004 FNEDPACLEGDGTSGSEFMSAFEDAATLSAGRFMYIFPHVWIFKRFFNIGSEKRLRESIK 825
FN DPACL G GTS +EFM AFEDAA LSAGRFMY FP ++ K+FFNIGSEK L ESI
Sbjct: 195 FNYDPACLGGGGTSAAEFMRAFEDAANLSAGRFMYAFPGLYKVKKFFNIGSEKTLNESIA 254
Query: 824 IVHEFAENIIKTRLQDK---GIKQEADLLSRFIAANDENAPNSLEFLRDIVISFILAGRD 654
IVH+FAE+II +RL++K I+ + DLLSRF+ D+N NS +FLRDI+ISFILAGRD
Sbjct: 255 IVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFM--GDQN--NSPQFLRDIIISFILAGRD 310
Query: 653 TTSTALTWFFWFI 615
TTS+ALTWFFW +
Sbjct: 311 TTSSALTWFFWIL 323
Score = 252 bits (644), Expect(2) = 0.0
Identities = 119/180 (66%), Positives = 142/180 (78%)
Frame = -3
Query: 610 RPHIERNILQELKTIRNDSGKCIGETYELDEFRQMNYLHAAISEAMRLYPPVAVDTKACL 431
R +++ IL EL+TIR + K IGE Y DE R M+YL AAISE +RLYPPV VDTKAC
Sbjct: 326 RHDVQQKILAELETIRTKARKEIGEMYSFDELRDMHYLQAAISETLRLYPPVPVDTKACR 385
Query: 430 ADDMLPDGTFIGKGWFATYQAYAMGRMENIWGKDCNEFVPERWLENGIFQPENPFRFPIF 251
DD+LPDGTFIGK WF TY YAMGRME+IWGKD E+ PERWLENG+ + E+PFRFPIF
Sbjct: 386 NDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKDYGEYSPERWLENGVCKTESPFRFPIF 445
Query: 250 HGGARMCLGKEMAYIQMKSIAASVIENFEINAVDKDTCPQHVLSLTLRMKDGLMVRVKER 71
H G RMCLGK+MAYIQMK IAA+VIE FE+ V+K P+H+LSLTLRM++GL V +K+R
Sbjct: 446 HAGPRMCLGKDMAYIQMKCIAAAVIEKFEVEMVEKKKSPKHLLSLTLRMENGLEVMIKKR 505
>ref|XP_002519477.1| cytochrome P450, putative [Ricinus communis]
gi|223541340|gb|EEF42891.1| cytochrome P450, putative
[Ricinus communis]
Length = 506
Score = 400 bits (1029), Expect(2) = 0.0
Identities = 192/296 (64%), Positives = 239/296 (80%)
Frame = -2
Query: 1508 KPSKTKPISEDNFESYPYVGNLPVFIKNRHRFLDWTTNILRNTPTNTITFRRPGKVQGVI 1329
+ S+ KP +++ F++YP +G LP F+KNRHRFLDWTT +L PTNT FRRPGKV GVI
Sbjct: 22 RSSQNKP-TKNGFKNYPILGALPDFLKNRHRFLDWTTEVLSCCPTNTSVFRRPGKVHGVI 80
Query: 1328 TANPLNVEHMLKTNFENYPKGNRFIFLLKDFLGRGIFNSDNELWRIQRKVASFEFSTRSL 1149
TANPLNVEH+LKT FENYPKG RFI LL+DFLG+GIFN+D ELWR+QRK AS+EF+T+SL
Sbjct: 81 TANPLNVEHILKTKFENYPKGERFISLLEDFLGQGIFNTDGELWRVQRKTASYEFNTKSL 140
Query: 1148 RNFVIENVRLELQTRLIPLLSNASKTKKTINIQDVLERFAFDNVCKVAFNEDPACLEGDG 969
RNF +ENV++EL TRL+P+L+ AS + +++Q++LERFAFDN+CK+AFN DP CL GDG
Sbjct: 141 RNFAMENVKVELSTRLVPILARASGMNQVLDLQEMLERFAFDNICKLAFNVDPGCLGGDG 200
Query: 968 TSGSEFMSAFEDAATLSAGRFMYIFPHVWIFKRFFNIGSEKRLRESIKIVHEFAENIIKT 789
T+ EFM AFE+A TLS+ RFMY P +W F +FF +G E+ L+ SIKIVHEFA+ II++
Sbjct: 201 TACGEFMQAFEEATTLSSARFMYALPFLWKFNKFFLLGPERTLKRSIKIVHEFADEIIQS 260
Query: 788 RLQDKGIKQEADLLSRFIAANDENAPNSLEFLRDIVISFILAGRDTTSTALTWFFW 621
RL KQ+ DLLSRFI D NSL+FLRDIVISFILAGRDTTS+AL+WFFW
Sbjct: 261 RLAQNRDKQDQDLLSRFIGDGD----NSLDFLRDIVISFILAGRDTTSSALSWFFW 312
Score = 271 bits (693), Expect(2) = 0.0
Identities = 124/179 (69%), Positives = 149/179 (83%)
Frame = -3
Query: 607 PHIERNILQELKTIRNDSGKCIGETYELDEFRQMNYLHAAISEAMRLYPPVAVDTKACLA 428
PH+ER IL+EL+ IR +GK IGETY +E R M+YL AAISE +RLYPPV VDTKAC
Sbjct: 318 PHVERKILEELEAIRARNGKNIGETYSFEELRDMHYLQAAISETLRLYPPVPVDTKACKN 377
Query: 427 DDMLPDGTFIGKGWFATYQAYAMGRMENIWGKDCNEFVPERWLENGIFQPENPFRFPIFH 248
DD LPDGTF+GK WF TY YAMGRME+IWGK+C EFVPERWL++GI + E+PF+FP+FH
Sbjct: 378 DDYLPDGTFVGKRWFITYHTYAMGRMESIWGKNCCEFVPERWLDDGICRQESPFKFPVFH 437
Query: 247 GGARMCLGKEMAYIQMKSIAASVIENFEINAVDKDTCPQHVLSLTLRMKDGLMVRVKER 71
G RMCLGK+MAYIQMKSIAASV+E F I+ +KD CP+H+L++TLRMKDGL V+VKER
Sbjct: 438 AGPRMCLGKDMAYIQMKSIAASVVERFHIDVQNKDKCPEHLLAMTLRMKDGLHVKVKER 496
>ref|XP_004167155.1| PREDICTED: cytochrome P450 94A1-like [Cucumis sativus]
Length = 511
Score = 419 bits (1077), Expect(2) = 0.0
Identities = 207/313 (66%), Positives = 255/313 (81%), Gaps = 3/313 (0%)
Frame = -2
Query: 1544 FSISLYFFFHNSKPSKTKPISEDNFESYPYVGNLPVFIKNRHRFLDWTTNILRNTPTNTI 1365
FS S+YF F S SK+KP + F+ YP+VG LP+F+ NRHRFLDW+T +LRN TNT
Sbjct: 17 FSFSIYFLFFPSTKSKSKP--QQGFKHYPFVGTLPLFLLNRHRFLDWSTEVLRNCRTNTS 74
Query: 1364 TFRRPGKVQGVITANPLNVEHMLKTNFENYPKGNRFIFLLKDFLGRGIFNSDNELWRIQR 1185
F+RPGKV GVITANPL VEH+LKT FENYPKG RFI LL+DFLGRGIFNSD E+W++QR
Sbjct: 75 VFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNSDGEIWKVQR 134
Query: 1184 KVASFEFSTRSLRNFVIENVRLELQTRLIPLLSNASKTKKTINIQDVLERFAFDNVCKVA 1005
K AS+EF+T+SLRNFV+ENVR+E+Q+RL+P+ A +T++ +++QDVLERFAFDNVCK+A
Sbjct: 135 KTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQDVLERFAFDNVCKLA 194
Query: 1004 FNEDPACLEGDGTSGSEFMSAFEDAATLSAGRFMYIFPHVWIFKRFFNIGSEKRLRESIK 825
FN D ACL G GTS +EFM AFEDAA LSAGRFMY FP ++ K+FFNIGSEK L ESI
Sbjct: 195 FNYDSACLGGGGTSAAEFMRAFEDAANLSAGRFMYAFPGLYKVKKFFNIGSEKTLNESIA 254
Query: 824 IVHEFAENIIKTRLQDK---GIKQEADLLSRFIAANDENAPNSLEFLRDIVISFILAGRD 654
IVH+FAE+II +RL++K I+ + DLLSRF+ D+N NS +FLRDI+ISFILAGRD
Sbjct: 255 IVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFM--GDQN--NSPQFLRDIIISFILAGRD 310
Query: 653 TTSTALTWFFWFI 615
TTS+ALTWFFW +
Sbjct: 311 TTSSALTWFFWIL 323
Score = 252 bits (644), Expect(2) = 0.0
Identities = 119/180 (66%), Positives = 142/180 (78%)
Frame = -3
Query: 610 RPHIERNILQELKTIRNDSGKCIGETYELDEFRQMNYLHAAISEAMRLYPPVAVDTKACL 431
R +++ IL EL+TIR + K IGE Y DE R M+YL AAISE +RLYPPV VDTKAC
Sbjct: 326 RHDVQQKILAELETIRTKARKEIGEMYSFDELRDMHYLQAAISETLRLYPPVPVDTKACR 385
Query: 430 ADDMLPDGTFIGKGWFATYQAYAMGRMENIWGKDCNEFVPERWLENGIFQPENPFRFPIF 251
DD+LPDGTFIGK WF TY YAMGRME+IWGKD E+ PERWLENG+ + E+PFRFPIF
Sbjct: 386 NDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKDYGEYSPERWLENGVCKTESPFRFPIF 445
Query: 250 HGGARMCLGKEMAYIQMKSIAASVIENFEINAVDKDTCPQHVLSLTLRMKDGLMVRVKER 71
H G RMCLGK+MAYIQMK IAA+VIE FE+ V+K P+H+LSLTLRM++GL V +K+R
Sbjct: 446 HAGPRMCLGKDMAYIQMKCIAAAVIEKFEVEMVEKKKSPKHLLSLTLRMENGLEVMIKKR 505
>ref|XP_003521105.1| PREDICTED: cytochrome P450 94A1-like [Glycine max]
Length = 492
Score = 402 bits (1032), Expect(2) = e-176
Identities = 192/309 (62%), Positives = 243/309 (78%)
Frame = -2
Query: 1541 SISLYFFFHNSKPSKTKPISEDNFESYPYVGNLPVFIKNRHRFLDWTTNILRNTPTNTIT 1362
S+ + + + ++ KP + F+ YP +G LP F+KNRHRFL+WTT +LR++PTNT
Sbjct: 10 SLLFFLYLYIQFLARKKPRNNKAFKDYPLIGTLPEFLKNRHRFLEWTTQVLRDSPTNTGV 69
Query: 1361 FRRPGKVQGVITANPLNVEHMLKTNFENYPKGNRFIFLLKDFLGRGIFNSDNELWRIQRK 1182
F RP K+ G++TANP NVEH+LKT F+NYPKG RFI LL+DFLG GIFNSD +LW++QRK
Sbjct: 70 FSRPYKLHGILTANPDNVEHVLKTKFDNYPKGERFIHLLQDFLGNGIFNSDGDLWKVQRK 129
Query: 1181 VASFEFSTRSLRNFVIENVRLELQTRLIPLLSNASKTKKTINIQDVLERFAFDNVCKVAF 1002
AS+EFST+SLRNFV++ V ELQTRL+P+LS AS+T K +++QD+LERFAFDNVCK+AF
Sbjct: 130 TASYEFSTKSLRNFVVDAVTFELQTRLLPILSKASETNKVLDLQDLLERFAFDNVCKLAF 189
Query: 1001 NEDPACLEGDGTSGSEFMSAFEDAATLSAGRFMYIFPHVWIFKRFFNIGSEKRLRESIKI 822
N DPACL GDGT+G EFM AFEDAA LS+GRFM I P VW K+ FN GSE+RLRESI
Sbjct: 190 NVDPACLGGDGTAGGEFMRAFEDAAVLSSGRFMSILPVVWKIKKLFNFGSERRLRESITT 249
Query: 821 VHEFAENIIKTRLQDKGIKQEADLLSRFIAANDENAPNSLEFLRDIVISFILAGRDTTST 642
VH+FA++II++RL+ K + DLLSRFI + S EFLRD+VISFILAGRDTTS+
Sbjct: 250 VHQFADSIIRSRLESKDQIGDEDLLSRFIRTEN----TSPEFLRDVVISFILAGRDTTSS 305
Query: 641 ALTWFFWFI 615
AL+WFFW +
Sbjct: 306 ALSWFFWIL 314
Score = 246 bits (628), Expect(2) = e-176
Identities = 115/181 (63%), Positives = 142/181 (78%)
Frame = -3
Query: 610 RPHIERNILQELKTIRNDSGKCIGETYELDEFRQMNYLHAAISEAMRLYPPVAVDTKACL 431
RP ++R I E++T+R++ K + +E ++M YL AAISE MRLYPPV VDT CL
Sbjct: 317 RPDVQRKIRDEIETVRSEKSK---GAFGYEEVKEMRYLQAAISETMRLYPPVPVDTMECL 373
Query: 430 ADDMLPDGTFIGKGWFATYQAYAMGRMENIWGKDCNEFVPERWLENGIFQPENPFRFPIF 251
DD+LPDGT +GKGWF TY YAMGRME++WGKDC EF PERWLEN + E+PFR+P+F
Sbjct: 374 NDDVLPDGTRVGKGWFVTYHTYAMGRMESVWGKDCTEFKPERWLEN---RAESPFRYPVF 430
Query: 250 HGGARMCLGKEMAYIQMKSIAASVIENFEINAVDKDTCPQHVLSLTLRMKDGLMVRVKER 71
H G RMCLGKEMAYIQMKSIAAS++E FEI A+DKDTCP+HVLSLT+R+K GL V V+ R
Sbjct: 431 HAGPRMCLGKEMAYIQMKSIAASLLERFEIEALDKDTCPEHVLSLTMRIKGGLPVSVRVR 490
Query: 70 S 68
+
Sbjct: 491 N 491