BLASTX nr result
ID: Coptis24_contig00008318
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00008318
(1612 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica] 325 e-139
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum] 316 e-135
ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]... 298 e-127
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 301 e-126
emb|CBI32510.3| unnamed protein product [Vitis vinifera] 293 e-124
>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
Length = 524
Score = 325 bits (833), Expect(2) = e-139
Identities = 171/358 (47%), Positives = 241/358 (67%), Gaps = 2/358 (0%)
Frame = -1
Query: 1570 MDFTMLIQWLLTSLATLVAFVLIWYSATSSSTRKINK--TAPVAAGALPIVGHLHMLMGR 1397
MD ML S+A+++AFV + Y+ SS T K K TAP+A GA P++GHLH+
Sbjct: 1 MDSLMLAYLFPISVASIIAFVFL-YNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSG 59
Query: 1396 ELPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAVFKRPRSKALKNLT 1217
ELPH++L+ MADKYG AF MK+G LVVS ++ KECF ND RP +KA + +T
Sbjct: 60 ELPHKMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMT 119
Query: 1216 YDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNN 1037
YD S F PYG W E+RK++ LLSN RLQ + VRASEV+ K LYD N
Sbjct: 120 YDNESVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYD---QCKNP 176
Query: 1036 NKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVVVDATLLTAK 857
+ AP+L++M KWFEE++ NVV R++ G+++ GSK + E+ E++HYKKV+ + L +
Sbjct: 177 SGSAPILIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEE-EAIHYKKVMDELLRLASL 235
Query: 856 LVVSDFFPSLGLVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVSGSEDDEQDFIDLT 677
+ SDF P LG VD QG+ S++K +K++DAIL W+EEHR KK S +E +QDF+D+
Sbjct: 236 SMFSDFAPLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKKNSVAE-SQQDFMDVM 294
Query: 676 LSMIDQTQHQGADVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNNRPVLRKAREEI 503
LS++++++ G D D IK+ C+ MI GG+D+T+V+LTW +S+LMNNR L+KAREE+
Sbjct: 295 LSIVEESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKAREEL 352
Score = 199 bits (507), Expect(2) = e-139
Identities = 90/151 (59%), Positives = 121/151 (80%)
Frame = -3
Query: 455 KAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPLLERVTVEDCEIGGYHLK 276
KAREE+D VG DR+V D D+ L ++ AI+KE+MR+ +G LLER T EDCEI G+H+K
Sbjct: 347 KAREELDALVGKDRQVEDSDLKNLVYMNAIVKETMRMYPLGTLLERETKEDCEIDGFHVK 406
Query: 275 TGSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGIT 96
G+R++VN+WK+Q DPN+W DP EFRPERFLT NA++++ GQH+ELLPFGAG R+CPG+
Sbjct: 407 GGTRLLVNVWKLQRDPNVWVDPTEFRPERFLTENADIDVGGQHFELLPFGAGRRVCPGVX 466
Query: 95 FALELIQLTLARLIHGFELGTPMDADVDMTE 3
FAL+ + L LARLIHG++L T + +VD+TE
Sbjct: 467 FALQFMHLVLARLIHGYDLNTLNEENVDLTE 497
>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
Length = 541
Score = 316 bits (810), Expect(2) = e-135
Identities = 166/371 (44%), Positives = 232/371 (62%), Gaps = 15/371 (4%)
Frame = -1
Query: 1570 MDFTMLIQWLLT------SLATLVAFVLIWY---------SATSSSTRKINKTAPVAAGA 1436
MDF+ L+ LL S+A L+A VL++ TS +KI P GA
Sbjct: 1 MDFSSLLLLLLNTWISAYSMAALLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAVTGA 60
Query: 1435 LPIVGHLHMLMGRELPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAV 1256
P+ GHLH+ E PH++LS +A+KYGP+F MK+G LVVS ++ KECF ND
Sbjct: 61 WPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTNDTLF 120
Query: 1255 FKRPRSKALKNLTYDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFI 1076
RP + A +TY S F PY P W E+RK++ LLSN RL+ + +R SEV
Sbjct: 121 SNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVSVCF 180
Query: 1075 KELYDLWSSNSNNNKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHY 896
KELYDL +N N+ GAP+ +++ +WF+E++ NV+ R++ GK++ GSK ED E++HY
Sbjct: 181 KELYDL--TNKKNDNGAPVPIDLKRWFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVHY 238
Query: 895 KKVVVDATLLTAKLVVSDFFPSLGLVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVS 716
KK++ + + L++ ++SD P LG +DY +GD ++K KEL++IL W+EEH+ KK
Sbjct: 239 KKIMDELSRLSSLTMLSDMVPLLGWLDYFKGDLRAMKRNGKELNSILQKWLEEHKSKK-- 296
Query: 715 GSEDDEQDFIDLTLSMIDQTQHQGADVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNN 536
S D QDF+D+ LS+ TQ G D DTFIK+ C+ MI GG++ST VALTW LS+LMNN
Sbjct: 297 -SSDARQDFMDVMLSISKDTQLYGHDQDTFIKATCLAMIMGGTNSTEVALTWILSLLMNN 355
Query: 535 RPVLRKAREEI 503
R L KAREEI
Sbjct: 356 RCALHKAREEI 366
Score = 195 bits (495), Expect(2) = e-135
Identities = 87/150 (58%), Positives = 120/150 (80%)
Frame = -3
Query: 455 KAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPLLERVTVEDCEIGGYHLK 276
KAREEID VG DR+V D DV L ++ AIIKE+MR+ +G LLER T EDCE+ G+++K
Sbjct: 361 KAREEIDLLVGKDRQVEDSDVKNLTYMNAIIKETMRLYPLGFLLERDTKEDCEVSGFNIK 420
Query: 275 TGSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGIT 96
G+R+++N+WK+Q DPN+W DP EF+PERFLT NA++++ GQH+ELLPFGAG R+CPG++
Sbjct: 421 GGTRLLINVWKLQRDPNVWTDPMEFKPERFLTENADIDVGGQHFELLPFGAGRRVCPGVS 480
Query: 95 FALELIQLTLARLIHGFELGTPMDADVDMT 6
FAL+ + L LARLIHG+++ T DVD++
Sbjct: 481 FALQFMHLVLARLIHGYDMETLNGEDVDLS 510
>ref|XP_002510298.1| cytochrome P450, putative [Ricinus communis]
gi|223550999|gb|EEF52485.1| cytochrome P450, putative
[Ricinus communis]
Length = 523
Score = 298 bits (762), Expect(2) = e-127
Identities = 158/354 (44%), Positives = 236/354 (66%), Gaps = 8/354 (2%)
Frame = -1
Query: 1540 LTSLATLVAFVLI---WYSATSSSTRKINKTAPVAAGALPIVGHLHMLMGRE-LPHQLLS 1373
LT++A +++F+ + W S ++ AP AG+ PI+GHLH+L G + L H+ L
Sbjct: 7 LTAIAVIISFIFVCSTWLRGKKKSVGSKSREAPEPAGSWPIIGHLHLLGGADKLLHRTLG 66
Query: 1372 NMADKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAVFKRPRSKALKNLTYDQASFGF 1193
++ADKYGPAF ++ GS ALVV+S +LAKECF ND+ + R + A K++ YD A FGF
Sbjct: 67 SLADKYGPAFNIRIGSHRALVVASKELAKECFTINDKTLASRSTTAATKHMCYDHAVFGF 126
Query: 1192 APYGPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNNNKGAPLLV 1013
APY W EMRK+ LLSN+RL+M +HV+ASEVD I++LY+LW+ N+ P++V
Sbjct: 127 APYSSHWREMRKIVMLELLSNRRLEMVKHVQASEVDLGIRKLYNLWA----QNRCLPVIV 182
Query: 1012 EMNKWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVVVDATLLTAKLVVSDFFP 833
E+ ++FE+L L+V+ R+V+GKR+ GS S+D E+ Y+K + L VVSD P
Sbjct: 183 ELKQFFEDLTLDVIVRVVAGKRYTGS----SDDDEARQYQKAISQFFHLMGIFVVSDALP 238
Query: 832 SLGLVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVSG--SEDDEQDFIDLTLSMIDQ 659
L +D L+G E ++K T+K+LDA+LA W++EHR ++VSG + +QDFID+ LS+ ++
Sbjct: 239 FLRWLD-LEGHEKAMKKTAKDLDAVLAGWLDEHRRRRVSGEVKSEGDQDFIDVMLSLEEK 297
Query: 658 TQHQG--ADVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNNRPVLRKAREEI 503
G D DT IKS C+ +I G SD+T+ L WA+S+L+NN+ L+ A+EE+
Sbjct: 298 GHLSGFQYDADTSIKSTCLALIAGASDTTTTTLVWAISLLLNNQLALKNAKEEL 351
Score = 184 bits (468), Expect(2) = e-127
Identities = 79/150 (52%), Positives = 117/150 (78%)
Frame = -3
Query: 452 AREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPLLERVTVEDCEIGGYHLKT 273
A+EE+++ +GT+R+V++ D+ L +L+A+IKE++R+ V PL+ R VEDC +GGYH+
Sbjct: 347 AKEELEKHIGTERQVDESDLKNLVYLQAVIKETLRLYPVAPLIPREFVEDCRVGGYHVPA 406
Query: 272 GSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGITF 93
G+R++VN+WK+Q DP LW F+PERFLT++A++++RG H+ELLPFG+G R CPG +F
Sbjct: 407 GTRLLVNVWKIQRDPMLWTKASAFQPERFLTSHADIDVRGHHFELLPFGSGRRSCPGASF 466
Query: 92 ALELIQLTLARLIHGFELGTPMDADVDMTE 3
AL + LTLAR +H F++ TPMD VDMTE
Sbjct: 467 ALHALHLTLARFLHAFDVATPMDQPVDMTE 496
>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|302142392|emb|CBI19595.3| unnamed protein product
[Vitis vinifera]
Length = 527
Score = 301 bits (772), Expect(2) = e-126
Identities = 163/326 (50%), Positives = 225/326 (69%), Gaps = 5/326 (1%)
Frame = -1
Query: 1465 NKTAPVAAGALPIVGHLHMLMGRE-LPHQLLSNMADKYGPAFMMKYGSQPALVVSSLKLA 1289
++ AP GA PI+GHLH+L G + L ++ L MADKYGPAF ++ GS+ A VVSS ++A
Sbjct: 35 SRDAPEPGGAWPIIGHLHLLGGGDQLLYRTLGAMADKYGPAFNIRLGSRRAFVVSSWEVA 94
Query: 1288 KECFVHNDRAVFKRPRSKALKNLTYDQASFGFAPYGPLWVEMRKVAKTNLLSNQRLQMQR 1109
KECF ND+A+ RP + A K++ Y+ A FGFAPY P W EMRK+A LLSN+RL++ +
Sbjct: 95 KECFTINDKALATRPTTVAAKHMGYNYAVFGFAPYSPFWREMRKIATLELLSNRRLEILK 154
Query: 1108 HVRASEVDAFIKELYDLWSSNSNNNKGAPLLVEMNKWFEELALNVVTRMVSGKRHIGSKA 929
HVR SEVD I+ELY LW NS+ PLLVE+N+W E++ LNV+ RMV+GKR+ G+ A
Sbjct: 155 HVRTSEVDMGIRELYGLWVKNSSR----PLLVELNRWLEDMTLNVIVRMVAGKRYFGA-A 209
Query: 928 RRSEDSESMHYKKVVVDATLLTAKLVVSDFFPSLGLVDYLQGDESSIKGTSKELDAILAT 749
S+ SE+ +K + L VVSD P L +D LQG E ++K T+KELD+IL
Sbjct: 210 AASDSSEARRCQKAISQFFRLIGIFVVSDALPFLWWLD-LQGHERAMKTTAKELDSILEG 268
Query: 748 WVEEHRHKKVSG--SEDDEQDFIDLTLSMIDQTQHQG--ADVDTFIKSMCVGMIFGGSDS 581
W+EEHR ++VS + EQDFID+ LS+ ++ + G D +T IKS C+ +I GGSD+
Sbjct: 269 WLEEHRQRRVSSLIKAEGEQDFIDVMLSLQEEGRLSGFQYDSETSIKSTCLALILGGSDT 328
Query: 580 TSVALTWALSILMNNRPVLRKAREEI 503
T+ LTWA+S+L+NNR L+KA+EE+
Sbjct: 329 TAGTLTWAISLLLNNRHALKKAQEEL 354
Score = 180 bits (456), Expect(2) = e-126
Identities = 82/152 (53%), Positives = 119/152 (78%), Gaps = 1/152 (0%)
Frame = -3
Query: 455 KAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPLL-ERVTVEDCEIGGYHL 279
KA+EE+D VG +R+V + DV L +L+AIIKE++R+ GPLL R ++DC + GY++
Sbjct: 349 KAQEELDLCVGMERQVEESDVKNLVYLQAIIKETLRLYPAGPLLGPREALDDCTVAGYNV 408
Query: 278 KTGSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGI 99
G+R+IVNIWK+Q DP++W +P F+PERFL +A+V+++GQ +EL+PFG+G R CPG+
Sbjct: 409 PAGTRLIVNIWKLQRDPSVWTNPCAFQPERFLNAHADVDVKGQQFELMPFGSGRRSCPGV 468
Query: 98 TFALELIQLTLARLIHGFELGTPMDADVDMTE 3
+FAL+++ LTLARL+H FEL TP+D VDMTE
Sbjct: 469 SFALQVLHLTLARLLHAFELSTPVDQPVDMTE 500
>emb|CBI32510.3| unnamed protein product [Vitis vinifera]
Length = 607
Score = 293 bits (751), Expect(2) = e-124
Identities = 157/348 (45%), Positives = 220/348 (63%), Gaps = 1/348 (0%)
Frame = -1
Query: 1543 LLTSLATLVAFVLIWYSATSSSTRKINKTAPVAAGALPIVGHLHMLMGRELPHQLLSNMA 1364
LL ++ L+A VL + +K K P +G P++GHLH+L +L H+ L +MA
Sbjct: 84 LLATICLLLATVL-----NAKGNKKRGKRPPEPSGRWPLIGHLHLLGADKLLHRTLGDMA 138
Query: 1363 DKYGPAFMMKYGSQPALVVSSLKLAKECFVHNDRAVFKRPRSKALKNLTYDQASFGFAPY 1184
DKYGP F ++ G + LVVSS ++AKEC+ +D+ RPRS A+K + YD SF FAPY
Sbjct: 139 DKYGPIFCVRLGLKKTLVVSSWEVAKECYTTSDKVFATRPRSLAIKLIGYDHGSFVFAPY 198
Query: 1183 GPLWVEMRKVAKTNLLSNQRLQMQRHVRASEVDAFIKELYDLWSSNSNNNKGAPLLVEMN 1004
GP W ++RK+A LLSN++L+M +HV+ SEV IKELY W+S NK P LVEM
Sbjct: 199 GPYWRDVRKLAMVELLSNRQLEMHKHVQDSEVKILIKELYGQWAS----NKDGPALVEMK 254
Query: 1003 KWFEELALNVVTRMVSGKRHIGSKARRSEDSESMHYKKVVVDATLLTAKLVVSDFFPSLG 824
+ F LALNVV R ++GKR+ G+ A E + KK D +L +VSD P LG
Sbjct: 255 ERFGNLALNVVVRAIAGKRYFGTHACGDEPKRA---KKAFEDFIILLGLFMVSDVIPFLG 311
Query: 823 LVDYLQGDESSIKGTSKELDAILATWVEEHRHKKVSGSEDD-EQDFIDLTLSMIDQTQHQ 647
+D ++G + +K +KE+D +L +WVEEHR ++S +++ EQDFI LS+ID Q
Sbjct: 312 WLDTMKGFTAEMKRVAKEVDYVLGSWVEEHRQNRLSANDNGAEQDFIHAMLSVIDDGQFS 371
Query: 646 GADVDTFIKSMCVGMIFGGSDSTSVALTWALSILMNNRPVLRKAREEI 503
G D DT IK C +I G+D+TS+ LTWALS+L+NNR L+KA+ E+
Sbjct: 372 GRDPDTIIKGTCSNLILAGNDTTSITLTWALSLLLNNRHALKKAQAEL 419
Score = 180 bits (457), Expect(2) = e-124
Identities = 82/152 (53%), Positives = 117/152 (76%), Gaps = 1/152 (0%)
Frame = -3
Query: 455 KAREEIDRQVGTDRKVNDLDVLKLDHLKAIIKESMRICLVGPL-LERVTVEDCEIGGYHL 279
KA+ E++ VG R+V+ D+ L +L+AI+KE++R+ GPL L +EDC + G+H+
Sbjct: 414 KAQAELEIHVGKHRQVDGSDIKNLVYLQAIVKETLRLYPPGPLSLPHEAMEDCTVAGFHI 473
Query: 278 KTGSRVIVNIWKMQHDPNLWPDPFEFRPERFLTTNANVELRGQHYELLPFGAGSRICPGI 99
+ G+R++VN+WK+ DP +W DP EF+PERFLT +A +++RG++YELLPFG+G R+CPGI
Sbjct: 474 QAGTRLLVNLWKLHRDPRVWLDPLEFQPERFLTKHAGLDVRGKNYELLPFGSGRRVCPGI 533
Query: 98 TFALELIQLTLARLIHGFELGTPMDADVDMTE 3
+FALEL LTLARL+HGFELG D+ VDMTE
Sbjct: 534 SFALELTHLTLARLLHGFELGAVADSPVDMTE 565