BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00007998
         (1779 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       606   e-171
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   570   e-160
ref|XP_002267599.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   498   e-138
ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   498   e-138
ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   488   e-135

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  606 bits (1563), Expect = e-171
 Identities = 308/506 (60%), Positives = 370/506 (73%)
 Frame = +3

Query: 114  MEFIFYFLLFLCSILLIKFLVPNKNKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXG 293
            ME + YFLLF+ SIL+IKFLVP + KN                                G
Sbjct: 1    MEILLYFLLFISSILVIKFLVPKRYKNLPPGPPSLPLIGHLHLFKKPLHRTLAKLSDKYG 60

Query: 294  QVMYLKFGSRPVIVVSSPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPN 473
             V+YL+FGSRPV+VVSSPSA E+CL+ KNDIIFANRP  L+GK+LGHDYTT++WA YGPN
Sbjct: 61   HVLYLQFGSRPVLVVSSPSAAEDCLA-KNDIIFANRPHLLMGKILGHDYTTLLWAPYGPN 119

Query: 474  WKNLRRISAVELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNVI 653
            W+NLR+ISA+ELLS +RLQ  S +R DEVKS+++RLS QG +++ VE+ +  F LT NV+
Sbjct: 120  WRNLRKISAIELLSANRLQSYSDIRHDEVKSMVQRLSGQGNEYRTVEMKTMIFELTLNVM 179

Query: 654  MRMMAGKRYYGEQVGDSEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEML 833
            M M+AGKRYYG QV + E+ KRFQE+V+ TL +S  SN+GDFLPI  WIG  KG + ++ 
Sbjct: 180  MTMIAGKRYYGGQVEELEQAKRFQEIVQGTLAISGASNIGDFLPILSWIG-IKGIEKKVW 238

Query: 834  GLKAKRDKFXXXXXXXXXXXXXXXKFDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIMW 1013
             LK KRDKF                   +EK   LI VLLSLQE EPE+YTD+++ GI+W
Sbjct: 239  NLKEKRDKFLQELIEEHRISRSGG-MQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIW 297

Query: 1014 DLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIV 1193
             L AAGTDTS  TMEWAMSLLLNNP V+KKAQ EID ++E GRL++ESDV KLPY+HSI+
Sbjct: 298  VLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNNLEQGRLINESDVNKLPYLHSII 357

Query: 1194 METLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTKFVPERF 1373
             ETLR+YP GPL++PHE+SEECIVGGY VP GTMLLVN+W I+ DPN+W EPTKF PERF
Sbjct: 358  TETLRIYPAGPLLVPHESSEECIVGGYKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERF 417

Query: 1374 LGLEGTRVGFKLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKL 1553
             G EGTR GFKLMPFG GRR CPGEGLA RV+ L L ALI CFDWERVGE+MVDMS+   
Sbjct: 418  DGFEGTRDGFKLMPFGSGRRGCPGEGLAMRVVALALGALIQCFDWERVGEEMVDMSEGP- 476

Query: 1554 GATMHKALPLEAKCRPRKNALNFIDK 1631
            G T+ K  PLEAKCRPR  ALNFI +
Sbjct: 477  GLTLPKVHPLEAKCRPRSTALNFISQ 502


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  570 bits (1469), Expect = e-160
 Identities = 291/507 (57%), Positives = 362/507 (71%), Gaps = 3/507 (0%)
 Frame = +3

Query: 123  IFYFLLFLCSILLIKFLVPN-KNKNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGQV 299
            + YFLLF+  +LL K +  + K+KN                                G V
Sbjct: 5    LVYFLLFITFLLLSKLVFSSPKHKNLPPSPPSLPIIGHLHLFKKPLYRTLTNISNQYGPV 64

Query: 300  MYLKFGSRPVIVVSSPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGPNWK 479
            +Y +FGSRPV+VVSSPSA EECL+ KNDI FANRPR +IGK  G++YTT+ WA YG NW+
Sbjct: 65   LYFQFGSRPVLVVSSPSAAEECLT-KNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWR 123

Query: 480  NLRRISAVELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNVIMR 659
            NLRRISA+E+ SP+R+QM S +R DEVKS+++RL   G ++  VE+ S FF LT N++MR
Sbjct: 124  NLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLV-AGYEYHKVEMKSVFFELTLNIMMR 182

Query: 660  MMAGKRYYGEQVGDSEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEMLGL 839
            M+AGKRYYGE V D E+ ++F+E+V ET  LS  SNVGDFLP+ RWIG  KG +  ++ L
Sbjct: 183  MIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLPLLRWIGY-KGIEKRLIRL 241

Query: 840  KAKRDKFXXXXXXXXXXXXXXXK-FDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIMWD 1016
            K KRD+F               K F  EEK+  LI VLLSLQE EPE+YTD++++G++W 
Sbjct: 242  KNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWI 301

Query: 1017 LFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSIVM 1196
            LFAAGTDTS  TMEWAMSLLLNNP ++KKAQ EIDL +EPGR +DESD+ KLPY+H I+ 
Sbjct: 302  LFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIIN 361

Query: 1197 ETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTKFVPERFL 1376
            ETLRMYP GPL+IPHE+SE+C +GGYN+P GTMLLVNLW I+NDPNLW EP KF PERF 
Sbjct: 362  ETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFE 421

Query: 1377 GLE-GTRVGFKLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQKL 1553
            G + G R GFKLMPFG GRR CPGEGLA RV+ LTL +L+ CFDW RVGE+MV+MS +  
Sbjct: 422  GYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMS-EGT 480

Query: 1554 GATMHKALPLEAKCRPRKNALNFIDKL 1634
            G T+ K  PLEA CRPR   LNF+ ++
Sbjct: 481  GLTLPKLHPLEAHCRPRSTMLNFLSQV 507


>ref|XP_002267599.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 508

 Score =  498 bits (1282), Expect = e-138
 Identities = 243/454 (53%), Positives = 334/454 (73%), Gaps = 6/454 (1%)
 Frame = +3

Query: 291  GQVMYLKFGSRPVIVVSSPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGP 470
            G +++L+ GSR  ++VSSPSA EECLS KND++FANRP+ L GK +G++YT+M WA+YG 
Sbjct: 61   GPIVFLRLGSRQTLLVSSPSAAEECLS-KNDVVFANRPQLLAGKYIGYNYTSMAWANYGD 119

Query: 471  NWKNLRRISAVELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNV 650
            +W+NLRRISA+E+LS SR+QMLS +R DEV+S++ RL + G   + V++ + FF +T NV
Sbjct: 120  HWRNLRRISALEILSTSRIQMLSGIRSDEVRSLLLRLLENGT--ETVDMKTTFFEVTMNV 177

Query: 651  IMRMMAGKRYYGEQVGDSEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEM 830
            +MRM+AGKRYYG  V + EE  +FQE++ +T RL D +N+GD+LP+ RW+G  KG +  +
Sbjct: 178  MMRMIAGKRYYGGNVVEVEETAKFQEIIEDTFRLGDTTNIGDYLPVLRWLGV-KGKEKGL 236

Query: 831  LGLKAKRDKFXXXXXXXXXXXXXXXKFDRE-----EKQTPLIQVLLSLQEAEPEHYTDDV 995
              L+ KRD+F                +        EK+  +I+VLLSLQE E E+YTD++
Sbjct: 237  RELQRKRDRFMQGLIEEHRTRMAKESYSSSSCRAGEKKKTMIEVLLSLQEKEAEYYTDEI 296

Query: 996  VKGIMWDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLP 1175
            ++G+M  L  AG DT+ +T+EWAMSLLLNNP VLKKAQ E+D  + P  L++ESD+ +LP
Sbjct: 297  IRGLMLALLGAGIDTTSATLEWAMSLLLNNPEVLKKAQMEMDNQLGPNHLIEESDLSQLP 356

Query: 1176 YVHSIVMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTK 1355
            Y+H I+ ET RMYP GP+ +PHE+S+EC+VGGY++PRGTMLLVN+W I+NDP +W EP K
Sbjct: 357  YLHCIIRETQRMYPAGPI-VPHESSKECMVGGYHIPRGTMLLVNIWGIQNDPEVWKEPRK 415

Query: 1356 FVPERF-LGLEGTRVGFKLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMV 1532
            F+PERF +GLEG   G +LMPFG GRR CPGEGLA R++ L L +LI CFDW+RVGE  V
Sbjct: 416  FLPERFEVGLEGEGHGLRLMPFGSGRRGCPGEGLAIRMVGLVLGSLIQCFDWKRVGEGKV 475

Query: 1533 DMSQQKLGATMHKALPLEAKCRPRKNALNFIDKL 1634
            DMS + +G T+ +A PL AKCRPR   +N + ++
Sbjct: 476  DMS-EGIGLTLPRAQPLLAKCRPRPALINLLSQI 508


>ref|XP_002267485.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 507

 Score =  498 bits (1281), Expect = e-138
 Identities = 245/454 (53%), Positives = 334/454 (73%), Gaps = 6/454 (1%)
 Frame = +3

Query: 291  GQVMYLKFGSRPVIVVSSPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGP 470
            G +++L+ G R  ++VSSPSA EECLS KND++FANRP+ L GK +G++YT+M WA+YG 
Sbjct: 61   GPIVFLRLGPRQTLLVSSPSAAEECLS-KNDVVFANRPQLLSGKYIGYNYTSMAWANYGD 119

Query: 471  NWKNLRRISAVELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNV 650
            +W+NLRRIS +E+LS SR+QMLS +R DEV+S++ RL + G   + V++ + FF +T NV
Sbjct: 120  HWRNLRRISTLEILSTSRIQMLSGIRSDEVRSLLLRLLENGA--ETVDMKTAFFEMTMNV 177

Query: 651  IMRMMAGKRYYGEQVGDSEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEM 830
            +MRM+AGKRYYG  V + EE  +FQE++ +T RL D +N+GD+LP+ RW+G  KG +  +
Sbjct: 178  MMRMIAGKRYYGGNVVEVEETAKFQEIIEDTFRLGDTTNIGDYLPVLRWLGV-KGKEKGL 236

Query: 831  LGLKAKRDKFXXXXXXXXXXXXXXXKFDRE-----EKQTPLIQVLLSLQEAEPEHYTDDV 995
              L+ KRD+F                +        EK+T +I+VLLSLQE E E+YTD++
Sbjct: 237  RELQRKRDRFMQGLIEEHRTRMAKESYSSSSCRVGEKKT-MIEVLLSLQEKEAEYYTDEI 295

Query: 996  VKGIMWDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLP 1175
            ++G+M  L  AGTDT+ +T+EWAMSLLLNNP VLKKAQ E+D  + P  L++ESD+ +LP
Sbjct: 296  IRGLMLALLGAGTDTTSATLEWAMSLLLNNPEVLKKAQMEMDNQLGPNHLIEESDLSQLP 355

Query: 1176 YVHSIVMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTK 1355
            Y+H I+ ET RMYP GP+ +PHE+S+EC+VGGY++PRGTMLLVN+W I+NDP +W EP K
Sbjct: 356  YLHCIIRETQRMYPAGPI-VPHESSKECMVGGYHIPRGTMLLVNIWGIQNDPKVWKEPRK 414

Query: 1356 FVPERF-LGLEGTRVGFKLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMV 1532
            F+PERF +GLEG   G +LMPFG GRR CPGEGLA R++ L L +LI CFDWERVGE  V
Sbjct: 415  FLPERFEVGLEGEGHGLRLMPFGSGRRGCPGEGLAIRMVGLVLGSLIQCFDWERVGEGKV 474

Query: 1533 DMSQQKLGATMHKALPLEAKCRPRKNALNFIDKL 1634
            DMS + +G T+ KA PL AKCRPR   +N + ++
Sbjct: 475  DMS-EGIGLTLPKAQPLLAKCRPRPALINVLSQI 507


>ref|XP_002285105.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 499

 Score =  488 bits (1256), Expect = e-135
 Identities = 239/448 (53%), Positives = 326/448 (72%)
 Frame = +3

Query: 291  GQVMYLKFGSRPVIVVSSPSAVEECLSSKNDIIFANRPRFLIGKLLGHDYTTMVWADYGP 470
            G +++L+FGSRPV++VSSPSA EEC + KND++FANRPR + GK LG++YT+M WA +G 
Sbjct: 61   GPILFLRFGSRPVLLVSSPSASEECFT-KNDVVFANRPRLIAGKHLGYNYTSMSWAPHGD 119

Query: 471  NWKNLRRISAVELLSPSRLQMLSAMRFDEVKSIIKRLSDQGCKHQNVELSSKFFALTYNV 650
            +W+NLRRIS+ E+LS +RLQ LS +R DEV+S+++ L     + Q VE+ S FF +T NV
Sbjct: 120  HWRNLRRISSFEILSSNRLQTLSGIRSDEVRSLVRWLFKN--QSQMVEMKSAFFEMTLNV 177

Query: 651  IMRMMAGKRYYGEQVGDSEEVKRFQELVRETLRLSDFSNVGDFLPIWRWIGGDKGFQNEM 830
            +M+M+ GKRYYGE +G+ EE + F+E+V ET +L+  +N+ DFLPI  W+G  KG +  +
Sbjct: 178  MMKMIGGKRYYGENIGEVEEARMFREMVSETFQLAGATNMVDFLPILGWLGL-KGTERRL 236

Query: 831  LGLKAKRDKFXXXXXXXXXXXXXXXKFDREEKQTPLIQVLLSLQEAEPEHYTDDVVKGIM 1010
            + L+ +R+ F                   E +Q  +I+VLLSLQE EPE+YTD++++G+M
Sbjct: 237  IKLQKRRESFIQNLIEEHRRKGSNC----EGRQKTMIEVLLSLQETEPEYYTDEIIRGLM 292

Query: 1011 WDLFAAGTDTSKSTMEWAMSLLLNNPHVLKKAQEEIDLHVEPGRLLDESDVEKLPYVHSI 1190
              +   GTDTS  TMEWA+SLLLNNP VLKKA +EID  +   RL++E D+ +LPY+ SI
Sbjct: 293  LSMLTGGTDTSAGTMEWALSLLLNNPKVLKKAHQEIDDRLGHDRLIEELDLAQLPYLRSI 352

Query: 1191 VMETLRMYPPGPLMIPHEASEECIVGGYNVPRGTMLLVNLWEIENDPNLWNEPTKFVPER 1370
            + ETLRMYP GPL++PHE+S+EC VGG+ +P+GTMLLVNLW I++D  +W +PT+F PER
Sbjct: 353  IKETLRMYPAGPLLVPHESSKECSVGGFRIPQGTMLLVNLWAIQSDHKIWGDPTEFRPER 412

Query: 1371 FLGLEGTRVGFKLMPFGVGRRKCPGEGLATRVITLTLAALIHCFDWERVGEQMVDMSQQK 1550
            F G+EG R GFK +PFG GRR CPGE LA R++ L L +LI CFDWERV EQMVDM++  
Sbjct: 413  FEGVEGDRDGFKFVPFGSGRRGCPGEALAIRIVGLALGSLIQCFDWERVDEQMVDMTEGG 472

Query: 1551 LGATMHKALPLEAKCRPRKNALNFIDKL 1634
             G T+ KA PL AKCRPR   +N   +L
Sbjct: 473  -GLTLPKAQPLLAKCRPRPTMVNLFSQL 499