BLASTX nr result
ID: Coptis24_contig00007981
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00007981
(1155 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003531881.1| PREDICTED: E3 ubiquitin-protein ligase At3g0... 158 5e-86
ref|XP_002330953.1| predicted protein [Populus trichocarpa] gi|2... 162 8e-84
gb|AFK43629.1| unknown [Lotus japonicus] 154 1e-82
ref|NP_001240211.1| uncharacterized protein LOC100777068 [Glycin... 154 2e-82
ref|XP_002281596.1| PREDICTED: E3 ubiquitin-protein ligase At3g0... 152 2e-82
>ref|XP_003531881.1| PREDICTED: E3 ubiquitin-protein ligase At3g02290-like [Glycine max]
Length = 227
Score = 158 bits (399), Expect(3) = 5e-86
Identities = 76/120 (63%), Positives = 88/120 (73%)
Frame = +3
Query: 546 PRCFHLQNRGLVSRREKGSSHLHEESEPLRINENENETDLEPLNPGKKWNGSDEGGSKGC 725
PR F Q GLVSRREKGSSHL+EESEPLR + + D E LN G KWN + E GSK
Sbjct: 90 PRFFRSQREGLVSRREKGSSHLNEESEPLR---GDVDADSESLNSGGKWNDTSEDGSKEY 146
Query: 726 FSDPPMKYRSTKVVSEIGYLYASSEEEDVCPTCLEEYTPENPKIITRCSHHFHLGCIYEW 905
S ++ S K+ + G +Y+SSEEEDVCPTCLEEYT ENPKI+T+CSHHFHLGCIYEW
Sbjct: 147 RSKSSVRLSSAKLTTGAGVVYSSSEEEDVCPTCLEEYTEENPKIVTKCSHHFHLGCIYEW 206
Score = 150 bits (378), Expect(3) = 5e-86
Identities = 67/88 (76%), Positives = 77/88 (87%)
Frame = +1
Query: 196 MGSVCCCLHVDDIEEFANPNSWVYRNCICLRCFIQNCLNVYTTLFRRGEVHAIPLSIEGA 375
MG+VCCC +VDD E++ NPNS VYRNC+CL CF+QN L VY ++FRRGEVHAIP SI+GA
Sbjct: 1 MGAVCCCFNVDDFEDYMNPNSSVYRNCMCLSCFVQNFLTVYESIFRRGEVHAIPSSIQGA 60
Query: 376 ASMTSTATQDNSLSDMYSSPPRPLPYDA 459
ASMTSTA+ DNSLSDMY SPPRPLPYDA
Sbjct: 61 ASMTSTASLDNSLSDMYRSPPRPLPYDA 88
Score = 58.9 bits (141), Expect(3) = 5e-86
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +2
Query: 1001 GCIYEWMERSESCPVCGKVMEFDETT 1078
GCIYEWMERS+SCPVCGKVM FDETT
Sbjct: 201 GCIYEWMERSDSCPVCGKVMVFDETT 226
>ref|XP_002330953.1| predicted protein [Populus trichocarpa] gi|222873147|gb|EEF10278.1|
predicted protein [Populus trichocarpa]
Length = 225
Score = 162 bits (409), Expect(3) = 8e-84
Identities = 77/120 (64%), Positives = 87/120 (72%)
Frame = +3
Query: 546 PRCFHLQNRGLVSRREKGSSHLHEESEPLRINENENETDLEPLNPGKKWNGSDEGGSKGC 725
PRC LQ GLVSRREKGSSH HEESEPLR ++N+ D E G KWN S G K
Sbjct: 89 PRCLRLQRDGLVSRREKGSSHSHEESEPLR---SDNDADSESFRTGDKWNASACEGGKEQ 145
Query: 726 FSDPPMKYRSTKVVSEIGYLYASSEEEDVCPTCLEEYTPENPKIITRCSHHFHLGCIYEW 905
S +K S K IGY+Y+SSEEEDVCPTCL+EYTPE+PKI+T+CSHHFHLGCIYEW
Sbjct: 146 HSRSSLKLSSAKATVGIGYVYSSSEEEDVCPTCLDEYTPEDPKIMTKCSHHFHLGCIYEW 205
Score = 139 bits (349), Expect(3) = 8e-84
Identities = 60/88 (68%), Positives = 77/88 (87%)
Frame = +1
Query: 196 MGSVCCCLHVDDIEEFANPNSWVYRNCICLRCFIQNCLNVYTTLFRRGEVHAIPLSIEGA 375
MGSVCCCLHVDD E++ NP + YRNC+CL CF+QN L+VYT++FRRG+VH++P SI+GA
Sbjct: 1 MGSVCCCLHVDDFEDYMNPENSEYRNCLCLSCFVQNFLHVYTSIFRRGQVHSVPSSIQGA 60
Query: 376 ASMTSTATQDNSLSDMYSSPPRPLPYDA 459
AS+TS++ DNSL++MY SPPRPLPYDA
Sbjct: 61 ASLTSSSL-DNSLAEMYRSPPRPLPYDA 87
Score = 58.9 bits (141), Expect(3) = 8e-84
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +2
Query: 1001 GCIYEWMERSESCPVCGKVMEFDETT 1078
GCIYEWMERS+SCPVCGKVM FDETT
Sbjct: 200 GCIYEWMERSDSCPVCGKVMVFDETT 225
>gb|AFK43629.1| unknown [Lotus japonicus]
Length = 229
Score = 154 bits (389), Expect(3) = 1e-82
Identities = 75/121 (61%), Positives = 88/121 (72%), Gaps = 1/121 (0%)
Frame = +3
Query: 546 PRCFHLQNRGLVSRREKGSSHLHEESEPLRINENENETDLEPLNPGKKWNG-SDEGGSKG 722
PR F Q GLVSRR+K SSH +EE+EPLR ++ E D E N G KWNG + + GSK
Sbjct: 90 PRYFRSQRDGLVSRRDKSSSHSNEETEPLR---SDVEVDPESFNSGDKWNGCAGDDGSKE 146
Query: 723 CFSDPPMKYRSTKVVSEIGYLYASSEEEDVCPTCLEEYTPENPKIITRCSHHFHLGCIYE 902
C S +K S K + +G +YASSEEEDVCPTCLEEYT ENPKI+T+CSHHFHLGCIYE
Sbjct: 147 CRSKSALKISSAKYTTGVGLIYASSEEEDVCPTCLEEYTTENPKIMTKCSHHFHLGCIYE 206
Query: 903 W 905
W
Sbjct: 207 W 207
Score = 145 bits (367), Expect(3) = 1e-82
Identities = 65/88 (73%), Positives = 74/88 (84%)
Frame = +1
Query: 196 MGSVCCCLHVDDIEEFANPNSWVYRNCICLRCFIQNCLNVYTTLFRRGEVHAIPLSIEGA 375
MGSVCCCL DD E++ NPNS VYRNC CL C IQN LNVYT++FRRGEVH++P SI+G
Sbjct: 1 MGSVCCCLSFDDFEDYVNPNSPVYRNCACLSCLIQNVLNVYTSIFRRGEVHSLPSSIQGT 60
Query: 376 ASMTSTATQDNSLSDMYSSPPRPLPYDA 459
ASMTS A+ DNSLSD+Y SPPRPLPYDA
Sbjct: 61 ASMTSAASLDNSLSDLYRSPPRPLPYDA 88
Score = 55.8 bits (133), Expect(3) = 1e-82
Identities = 22/26 (84%), Positives = 25/26 (96%)
Frame = +2
Query: 1001 GCIYEWMERSESCPVCGKVMEFDETT 1078
GCIYEWMERS++CPVCGKVM FDE+T
Sbjct: 202 GCIYEWMERSDNCPVCGKVMLFDEST 227
>ref|NP_001240211.1| uncharacterized protein LOC100777068 [Glycine max]
gi|255647555|gb|ACU24241.1| unknown [Glycine max]
Length = 229
Score = 154 bits (389), Expect(3) = 2e-82
Identities = 77/121 (63%), Positives = 90/121 (74%), Gaps = 1/121 (0%)
Frame = +3
Query: 546 PRCFHLQNRGLVSRREKGSSHLHEESEPLRINENENETDLEPLNPGKKWNGSD-EGGSKG 722
PR F Q GLVSRREKGSSH +EE+EPLR ++ + D E LN G KWN S E GSK
Sbjct: 90 PRYFRSQRDGLVSRREKGSSHSNEETEPLR---SDADLDPESLNSGDKWNESACEAGSKE 146
Query: 723 CFSDPPMKYRSTKVVSEIGYLYASSEEEDVCPTCLEEYTPENPKIITRCSHHFHLGCIYE 902
S ++ +STK + +G +YASSEEEDVCPTCLEEYT ENPKI+T+CSHHFHLGCIYE
Sbjct: 147 YHSKSSLRLQSTKYSTGVGLVYASSEEEDVCPTCLEEYTKENPKIMTKCSHHFHLGCIYE 206
Query: 903 W 905
W
Sbjct: 207 W 207
Score = 143 bits (360), Expect(3) = 2e-82
Identities = 63/88 (71%), Positives = 75/88 (85%)
Frame = +1
Query: 196 MGSVCCCLHVDDIEEFANPNSWVYRNCICLRCFIQNCLNVYTTLFRRGEVHAIPLSIEGA 375
MGSVCCCL DD E++ NPNS VYRNC+CL C IQN L+VY+++FRRG+ H+IP SI+GA
Sbjct: 1 MGSVCCCLSFDDFEDYVNPNSPVYRNCVCLSCLIQNLLSVYSSIFRRGDAHSIPSSIQGA 60
Query: 376 ASMTSTATQDNSLSDMYSSPPRPLPYDA 459
AS+TS A+ DNSLSDMY SPPRPLPYDA
Sbjct: 61 ASITSAASLDNSLSDMYRSPPRPLPYDA 88
Score = 57.8 bits (138), Expect(3) = 2e-82
Identities = 23/26 (88%), Positives = 25/26 (96%)
Frame = +2
Query: 1001 GCIYEWMERSESCPVCGKVMEFDETT 1078
GCIYEWMERS++CPVCGKVM FDETT
Sbjct: 202 GCIYEWMERSDNCPVCGKVMVFDETT 227
>ref|XP_002281596.1| PREDICTED: E3 ubiquitin-protein ligase At3g02290 [Vitis vinifera]
gi|298204451|emb|CBI16931.3| unnamed protein product
[Vitis vinifera]
Length = 225
Score = 152 bits (385), Expect(3) = 2e-82
Identities = 75/120 (62%), Positives = 88/120 (73%)
Frame = +3
Query: 546 PRCFHLQNRGLVSRREKGSSHLHEESEPLRINENENETDLEPLNPGKKWNGSDEGGSKGC 725
PR F LQ GLVSRREKGSSH HEESEPLR ++ + D E L KWN SD+G SK
Sbjct: 90 PRNFRLQRDGLVSRREKGSSHSHEESEPLR---SDTDGDSESLITRDKWNESDDG-SKEQ 145
Query: 726 FSDPPMKYRSTKVVSEIGYLYASSEEEDVCPTCLEEYTPENPKIITRCSHHFHLGCIYEW 905
+K+ K +G++Y+SSE+EDVCPTCLEEYTPENPKI+T+CSHHFHLGCIYEW
Sbjct: 146 RPRSSVKHSMAKTTMGLGFIYSSSEDEDVCPTCLEEYTPENPKIMTKCSHHFHLGCIYEW 205
Score = 143 bits (361), Expect(3) = 2e-82
Identities = 62/87 (71%), Positives = 77/87 (88%)
Frame = +1
Query: 196 MGSVCCCLHVDDIEEFANPNSWVYRNCICLRCFIQNCLNVYTTLFRRGEVHAIPLSIEGA 375
MGSVCCCL V+D +++ NPNS +YRNC+CL CF+QN LNVYT+LFRRGEV++IP SI+GA
Sbjct: 1 MGSVCCCLRVEDFDDYVNPNSSIYRNCMCLSCFVQNFLNVYTSLFRRGEVNSIPSSIQGA 60
Query: 376 ASMTSTATQDNSLSDMYSSPPRPLPYD 456
AS++ST + D+SLSDMY SPPRPLPYD
Sbjct: 61 ASLSSTTSLDDSLSDMYRSPPRPLPYD 87
Score = 58.9 bits (141), Expect(3) = 2e-82
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +2
Query: 1001 GCIYEWMERSESCPVCGKVMEFDETT 1078
GCIYEWMERSE+CPVCGKVM FDETT
Sbjct: 200 GCIYEWMERSETCPVCGKVMMFDETT 225