BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00007642
         (1918 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270943.2| PREDICTED: sphingosine kinase B-like [Vitis ...   626   e-177
emb|CBI23326.3| unnamed protein product [Vitis vinifera]              626   e-177
ref|XP_002524674.1| Sphingosine kinase, putative [Ricinus commun...   597   e-168
ref|XP_002317243.1| predicted protein [Populus trichocarpa] gi|2...   597   e-168
ref|XP_003545841.1| PREDICTED: sphingosine kinase A-like [Glycin...   581   e-163

>ref|XP_002270943.2| PREDICTED: sphingosine kinase B-like [Vitis vinifera]
          Length = 489

 Score =  626 bits (1614), Expect = e-177
 Identities = 302/495 (61%), Positives = 378/495 (76%), Gaps = 1/495 (0%)
 Frame = +1

Query: 70   EQQPISSQVKINKXXXXXXXXXXDGKLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVK 249
            E QP+ S   +            DGK+RW+DG  +RC+ +E+EVLGF     EG  +R++
Sbjct: 5    ESQPLISDRFLINGTLTSVDFRDDGKIRWSDGHTDRCLAVEKEVLGFA---SEGSRIRIR 61

Query: 250  ALVEKESGSCXXXXXXXXXXXXXXXXXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKR 429
            A+VE   G C                  +DF+F+    DS +++CQK RE++DSL RPKR
Sbjct: 62   AIVENGGGLCCVGSRGKLVR--------KDFVFEIASADSHRLWCQKLREYIDSLGRPKR 113

Query: 430  LLVIVNPFGGKRIALKIYNDEVKPLLDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVV 609
            L V VNPFGGK+ A KI+ D V PLL  ADI+ T+QET++QLHAK + ++LDLS+YDG++
Sbjct: 114  LFVFVNPFGGKKSASKIFLDVVSPLLKDADIQLTIQETKHQLHAKAVTQTLDLSKYDGLI 173

Query: 610  CVSGDGILVEVVNGLLERKDWDTAIKMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLA 789
            CVSGDGILVEVVNGLLER DWD+AIKMP+G++ AGTGNGM KSLLDSV + CS  NAVLA
Sbjct: 174  CVSGDGILVEVVNGLLERDDWDSAIKMPIGVIPAGTGNGMAKSLLDSVGDPCSPINAVLA 233

Query: 790  IVRGHKRALDVATVLQGETRFFSVLMLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFN 969
            I+RGHK +LDVAT+LQGET+FFSVLMLAWGL+ADIDIESEKYRWMGS+RLDFY L R+ +
Sbjct: 234  IIRGHKCSLDVATILQGETKFFSVLMLAWGLVADIDIESEKYRWMGSARLDFYALQRILH 293

Query: 970  LRKYNGRVSFVPAPGFEAYGNPCDQKVGCTN-IDILFRDQQDTVKVQEHGYQGPVVLMEE 1146
            LRKYNG +SFVPAPGFEA+G P   K   T+ ++     Q++ +KVQ+HGYQGP + +E+
Sbjct: 294  LRKYNGCISFVPAPGFEAFGEPSTYKGESTSGLNTFNPSQEEPIKVQQHGYQGPNIELEK 353

Query: 1147 LKWRAVEGPFVSVWLHNVPWGSEDTMAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLND 1326
            L+WR ++GPFVS+WLHNVPWG EDT+AAPDA+FSDGYLDLI+I+DCPK  LL L+++LN 
Sbjct: 354  LQWRTIDGPFVSIWLHNVPWGGEDTLAAPDAKFSDGYLDLIIIRDCPKLPLLALMSELNK 413

Query: 1327 GTHVRSPYVMYLKVKAFILEPGHRVGDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGP 1506
            G H++SPYVMY KVKAF+LEPG    DP+KGGIIDSDGEVLARG G YKC+ K LM Y  
Sbjct: 414  GNHIKSPYVMYFKVKAFVLEPGPCTEDPTKGGIIDSDGEVLARGNGTYKCDQKALMAYDN 473

Query: 1507 MKITVDQGLATLFCP 1551
            ++I VDQGLAT+F P
Sbjct: 474  LQIAVDQGLATVFSP 488


>emb|CBI23326.3| unnamed protein product [Vitis vinifera]
          Length = 506

 Score =  626 bits (1614), Expect = e-177
 Identities = 302/495 (61%), Positives = 378/495 (76%), Gaps = 1/495 (0%)
 Frame = +1

Query: 70   EQQPISSQVKINKXXXXXXXXXXDGKLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVK 249
            E QP+ S   +            DGK+RW+DG  +RC+ +E+EVLGF     EG  +R++
Sbjct: 22   ESQPLISDRFLINGTLTSVDFRDDGKIRWSDGHTDRCLAVEKEVLGFA---SEGSRIRIR 78

Query: 250  ALVEKESGSCXXXXXXXXXXXXXXXXXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKR 429
            A+VE   G C                  +DF+F+    DS +++CQK RE++DSL RPKR
Sbjct: 79   AIVENGGGLCCVGSRGKLVR--------KDFVFEIASADSHRLWCQKLREYIDSLGRPKR 130

Query: 430  LLVIVNPFGGKRIALKIYNDEVKPLLDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVV 609
            L V VNPFGGK+ A KI+ D V PLL  ADI+ T+QET++QLHAK + ++LDLS+YDG++
Sbjct: 131  LFVFVNPFGGKKSASKIFLDVVSPLLKDADIQLTIQETKHQLHAKAVTQTLDLSKYDGLI 190

Query: 610  CVSGDGILVEVVNGLLERKDWDTAIKMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLA 789
            CVSGDGILVEVVNGLLER DWD+AIKMP+G++ AGTGNGM KSLLDSV + CS  NAVLA
Sbjct: 191  CVSGDGILVEVVNGLLERDDWDSAIKMPIGVIPAGTGNGMAKSLLDSVGDPCSPINAVLA 250

Query: 790  IVRGHKRALDVATVLQGETRFFSVLMLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFN 969
            I+RGHK +LDVAT+LQGET+FFSVLMLAWGL+ADIDIESEKYRWMGS+RLDFY L R+ +
Sbjct: 251  IIRGHKCSLDVATILQGETKFFSVLMLAWGLVADIDIESEKYRWMGSARLDFYALQRILH 310

Query: 970  LRKYNGRVSFVPAPGFEAYGNPCDQKVGCTN-IDILFRDQQDTVKVQEHGYQGPVVLMEE 1146
            LRKYNG +SFVPAPGFEA+G P   K   T+ ++     Q++ +KVQ+HGYQGP + +E+
Sbjct: 311  LRKYNGCISFVPAPGFEAFGEPSTYKGESTSGLNTFNPSQEEPIKVQQHGYQGPNIELEK 370

Query: 1147 LKWRAVEGPFVSVWLHNVPWGSEDTMAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLND 1326
            L+WR ++GPFVS+WLHNVPWG EDT+AAPDA+FSDGYLDLI+I+DCPK  LL L+++LN 
Sbjct: 371  LQWRTIDGPFVSIWLHNVPWGGEDTLAAPDAKFSDGYLDLIIIRDCPKLPLLALMSELNK 430

Query: 1327 GTHVRSPYVMYLKVKAFILEPGHRVGDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGP 1506
            G H++SPYVMY KVKAF+LEPG    DP+KGGIIDSDGEVLARG G YKC+ K LM Y  
Sbjct: 431  GNHIKSPYVMYFKVKAFVLEPGPCTEDPTKGGIIDSDGEVLARGNGTYKCDQKALMAYDN 490

Query: 1507 MKITVDQGLATLFCP 1551
            ++I VDQGLAT+F P
Sbjct: 491  LQIAVDQGLATVFSP 505


>ref|XP_002524674.1| Sphingosine kinase, putative [Ricinus communis]
            gi|223536035|gb|EEF37693.1| Sphingosine kinase, putative
            [Ricinus communis]
          Length = 479

 Score =  597 bits (1540), Expect = e-168
 Identities = 302/492 (61%), Positives = 362/492 (73%)
 Frame = +1

Query: 76   QPISSQVKINKXXXXXXXXXXDGKLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKAL 255
            QPI S   I             G LRW D   +RC+T+E+EVLGF IE   G  +RVK +
Sbjct: 6    QPIISDQVIVNGATTLLTLTAGGILRWTDRG-QRCLTVEKEVLGFVIE---GSKIRVKTI 61

Query: 256  VEKESGSCXXXXXXXXXXXXXXXXXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLL 435
            ++   G C                  +DF+FQ   EDS ++ C + R++LDSL RPKRLL
Sbjct: 62   IDNGDGICCAGNSGALVR--------KDFVFQPLTEDSQRVLCNRLRDYLDSLGRPKRLL 113

Query: 436  VIVNPFGGKRIALKIYNDEVKPLLDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCV 615
            V VNPFGGKR A KI+ D VKPLL+ AD++ T+QET++QLHAKE+  +LD+S+YDG+VCV
Sbjct: 114  VFVNPFGGKRSASKIFFDTVKPLLEDADVQITLQETKHQLHAKEVTSTLDISKYDGIVCV 173

Query: 616  SGDGILVEVVNGLLERKDWDTAIKMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIV 795
            SGDGILVEVVNGLL R+DW  AIK+PLG+V AGT NGM KSLLDSV E C  SNAVLAI+
Sbjct: 174  SGDGILVEVVNGLLAREDWRDAIKLPLGMVPAGTSNGMAKSLLDSVGEPCKASNAVLAII 233

Query: 796  RGHKRALDVATVLQGETRFFSVLMLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLR 975
            RGHK +LDVAT+LQGET+FFSVLML+WGL+ADIDIESEKYRWMGS+R+DFY + R+F+LR
Sbjct: 234  RGHKCSLDVATILQGETKFFSVLMLSWGLVADIDIESEKYRWMGSARIDFYAVQRIFHLR 293

Query: 976  KYNGRVSFVPAPGFEAYGNPCDQKVGCTNIDILFRDQQDTVKVQEHGYQGPVVLMEELKW 1155
             YNG +SFVPAPGFE YG P       T+       Q+  +K Q HGYQGP V +  L W
Sbjct: 294  HYNGCISFVPAPGFETYGVPTSYNAESTS------KQEQPLKTQ-HGYQGPDVNLVNLDW 346

Query: 1156 RAVEGPFVSVWLHNVPWGSEDTMAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTH 1335
            R + GPFVS+WLHNVPWG ED MAAPDA+FSDGYLDLI+I+ CPK SLL L+T LN+G H
Sbjct: 347  RMISGPFVSIWLHNVPWGGEDVMAAPDAKFSDGYLDLILIQQCPKLSLLALMTALNNGDH 406

Query: 1336 VRSPYVMYLKVKAFILEPGHRVGDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKI 1515
            V+SPYV+YLKVKAFILEPG R  DP+KGGIID DGEVLARG G+YKC+ K LM Y  + +
Sbjct: 407  VKSPYVIYLKVKAFILEPGPRTDDPTKGGIIDVDGEVLARGNGSYKCDQKSLMVYDKLHM 466

Query: 1516 TVDQGLATLFCP 1551
             VDQGLATLF P
Sbjct: 467  MVDQGLATLFSP 478


>ref|XP_002317243.1| predicted protein [Populus trichocarpa] gi|222860308|gb|EEE97855.1|
            predicted protein [Populus trichocarpa]
          Length = 494

 Score =  597 bits (1540), Expect = e-168
 Identities = 297/491 (60%), Positives = 369/491 (75%), Gaps = 1/491 (0%)
 Frame = +1

Query: 82   ISSQVKINKXXXXXXXXXXDGKLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVKALVE 261
            IS QVK+N           +GKL+W     +  +T+E+EVLGF I+   G  + +KA+ E
Sbjct: 11   ISDQVKVN-GNLTPLTLTTEGKLQWTTEKGQHSLTVEKEVLGFTIQ---GSKIIIKAVTE 66

Query: 262  KESGSCXXXXXXXXXXXXXXXXXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKRLLVI 441
            K  G                    +DF+F+   ++S  ++CQ  R++++SL RPKRL + 
Sbjct: 67   KRDG------IFCSASAGGGALARKDFVFEPLSDESLHLWCQHLRDYINSLGRPKRLFIF 120

Query: 442  VNPFGGKRIALKIYNDEVKPLLDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVVCVSG 621
            VNPFGGK+ ALKIY D VKPLL+ AD++ T+QET+YQLHAKE+VR++DL++YDG+VCVSG
Sbjct: 121  VNPFGGKKSALKIYFDVVKPLLEDADVQITVQETKYQLHAKEVVRAMDLTKYDGIVCVSG 180

Query: 622  DGILVEVVNGLLERKDWDTAIKMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLAIVRG 801
            DGILVEVVNGLLER+DW+ AIKMPLG V AGTGNGMVKS LDS  E C+ SNAV+AI+RG
Sbjct: 181  DGILVEVVNGLLEREDWNAAIKMPLGTVPAGTGNGMVKSTLDSAGEPCTASNAVVAIIRG 240

Query: 802  HKRALDVATVLQGETRFFSVLMLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFNLRKY 981
            HK +LDVAT+LQG+T+FFSVLM AWGL+ADIDIESEKYRWMGS+RLDFYGL R+  LR+Y
Sbjct: 241  HKCSLDVATILQGDTKFFSVLMFAWGLVADIDIESEKYRWMGSARLDFYGLQRMLCLRQY 300

Query: 982  NGRVSFVPAPGFEAYGNPCDQKVGCTNIDILFR-DQQDTVKVQEHGYQGPVVLMEELKWR 1158
            +GR+SFVPAPGFEAYG P       T+        Q+  VK +++ YQGP V +  L+WR
Sbjct: 301  SGRISFVPAPGFEAYGEPTRYNGEFTSTQSSINPGQEQHVKAEQYSYQGPDVDLTNLEWR 360

Query: 1159 AVEGPFVSVWLHNVPWGSEDTMAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLNDGTHV 1338
             + GPF+SVWLHNVPWG E TMAAPDA+F+DG LDLI+IKDCPK  LL L+T L+DG HV
Sbjct: 361  TINGPFISVWLHNVPWGGEGTMAAPDAKFADGNLDLILIKDCPKLGLLALMTNLSDGGHV 420

Query: 1339 RSPYVMYLKVKAFILEPGHRVGDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYGPMKIT 1518
            +SP+VMYLKVKAFILEPG R  DP++GGIID DGEVLARG G YK + K LMTY  ++IT
Sbjct: 421  KSPHVMYLKVKAFILEPGQRTKDPTRGGIIDIDGEVLARGNGTYKHDQKTLMTYDKLQIT 480

Query: 1519 VDQGLATLFCP 1551
            +DQGLATLFCP
Sbjct: 481  MDQGLATLFCP 491


>ref|XP_003545841.1| PREDICTED: sphingosine kinase A-like [Glycine max]
          Length = 488

 Score =  581 bits (1497), Expect = e-163
 Identities = 293/496 (59%), Positives = 362/496 (72%), Gaps = 2/496 (0%)
 Frame = +1

Query: 70   EQQPISSQVKINKXXXXXXXXXXDGKLRWNDGSRERCITIEREVLGFGIEEKEGFLVRVK 249
            +Q+P+ S                DG+L W++G  +RC++IE++VLG       G  +++K
Sbjct: 4    QQRPLMSDRVTVNGSVTPLALLADGRLWWSEGI-QRCLSIEKDVLGI---VASGPYIKIK 59

Query: 250  ALVEKESGSCXXXXXXXXXXXXXXXXXXEDFLFQFFDEDSFKIFCQKFREFLDSLDRPKR 429
            +LVE   G C                   D +F    E+S +++C K REF+DSL RPKR
Sbjct: 60   SLVETRDGCCITSAPARLVR--------NDVVFMPSSEESHRLWCHKLREFIDSLGRPKR 111

Query: 430  LLVIVNPFGGKRIALKIYNDEVKPLLDAADIEYTMQETQYQLHAKEIVRSLDLSRYDGVV 609
            LLV VNPFGGK+ A KI+ ++VKPL + A I+ T+QET++QLHAKE+ RSLD+++YDG+V
Sbjct: 112  LLVFVNPFGGKKSATKIFAEQVKPLFEDAHIQITVQETKHQLHAKEVARSLDITKYDGIV 171

Query: 610  CVSGDGILVEVVNGLLERKDWDTAIKMPLGIVLAGTGNGMVKSLLDSVDECCSVSNAVLA 789
            CVSGDGILVEVVNGLL+R+DWDTAIKMPLG+V AGTGNGM KSLLDSV + C V+NAVLA
Sbjct: 172  CVSGDGILVEVVNGLLQREDWDTAIKMPLGVVPAGTGNGMAKSLLDSVGDPCEVANAVLA 231

Query: 790  IVRGHKRALDVATVLQGETRFFSVLMLAWGLIADIDIESEKYRWMGSSRLDFYGLMRVFN 969
            I+RG KR LDVAT+ QGETRFFS+LMLAWGL+ADIDIESEKYRWMGS+RLDFYGL R+ N
Sbjct: 232  IIRGSKRPLDVATITQGETRFFSILMLAWGLVADIDIESEKYRWMGSARLDFYGLCRLLN 291

Query: 970  LRKYNGRVSFVPAPGFEAYGNPCDQKVGCTNIDILFRD--QQDTVKVQEHGYQGPVVLME 1143
            LR+Y G VSFVPAPG+EA+G P       T       D  + +   +Q   Y GP + +E
Sbjct: 292  LRQYIGCVSFVPAPGYEAFGEPTSYPGKSTTSKGSNNDPSEAERANLQRLCYLGPEINLE 351

Query: 1144 ELKWRAVEGPFVSVWLHNVPWGSEDTMAAPDAQFSDGYLDLIVIKDCPKTSLLMLITKLN 1323
             L WR + GPF+SVWLHNVPWG+EDTMAAPDA+FSDGYLDLI+IK+CP   LL ++++LN
Sbjct: 352  NLNWRVINGPFISVWLHNVPWGAEDTMAAPDAKFSDGYLDLIIIKNCPTLPLLSMMSELN 411

Query: 1324 DGTHVRSPYVMYLKVKAFILEPGHRVGDPSKGGIIDSDGEVLARGEGAYKCEVKDLMTYG 1503
             G HV+SPYV YLKVKAF L+PG R  D  K GIIDSDGEVLARG+G YKCE K LM Y 
Sbjct: 412  KGGHVKSPYVTYLKVKAFNLQPGPRTKDQEKEGIIDSDGEVLARGKGTYKCEQKTLMAYD 471

Query: 1504 PMKITVDQGLATLFCP 1551
             ++ITVDQGLATLF P
Sbjct: 472  KLQITVDQGLATLFTP 487