BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00006772
         (1813 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAJ40864.1| tetrahydroberberine oxidase [Coptis japonica]        1006   0.0  
gb|ADY15026.1| (S)-tetrahydroprotoberberine oxidase [Berberis wi...   684   0.0  
ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|2...   574   e-161
ref|XP_002277310.1| PREDICTED: reticuline oxidase-like protein-l...   552   e-154
ref|XP_002277294.1| PREDICTED: reticuline oxidase-like protein-l...   551   e-154

>dbj|BAJ40864.1| tetrahydroberberine oxidase [Coptis japonica]
          Length = 540

 Score = 1006 bits (2602), Expect = 0.0
 Identities = 507/567 (89%), Positives = 512/567 (90%)
 Frame = +3

Query: 99   MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPVYNPSSTSYTSI 278
            MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIP+YNPSSTSYTSI
Sbjct: 1    MMGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPIYNPSSTSYTSI 60

Query: 279  LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSSSVA 458
            LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGL IRTRSGGHDFEGSS VA
Sbjct: 61   LHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLLIRTRSGGHDFEGSSFVA 120

Query: 459  PVPFVLLDLIRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG 638
             VPFVLLDLI LRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG
Sbjct: 121  TVPFVLLDLIHLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGG 180

Query: 639  HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA 818
            HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA
Sbjct: 181  HISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILA 240

Query: 819  WKXXXXXXXXXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE 998
            WK                   EQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE
Sbjct: 241  WKIRLVPVPPTVTTFVVPRVLEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPE 300

Query: 999  KKTIVISFLGFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLENDLSL 1178
            KKTIVISFLGFYLGAPEK LQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLE DLSL
Sbjct: 301  KKTIVISFLGFYLGAPEKTLQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLETDLSL 360

Query: 1179 LLERKNPIPPKNRYMSKSDYVHEQVSEAVLEGIWKRFDGIDEPEIIMSPYGGKMNEIEES 1358
            LLERKNPIPPKNRYMSKSDYVHE VSEAVLEGIWKRF+ +DEPEIIMSPYGGKMNEIEES
Sbjct: 361  LLERKNPIPPKNRYMSKSDYVHEPVSEAVLEGIWKRFNEVDEPEIIMSPYGGKMNEIEES 420

Query: 1359 AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVXXXXXXXXXXXXXX 1538
            AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTP                
Sbjct: 421  AIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTP---------------- 464

Query: 1539 XXXXXXXXXXXYVSKSPRCSYLNFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSV 1718
                       YVSK+PRCSYLNFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSV
Sbjct: 465  -----------YVSKNPRCSYLNFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSV 513

Query: 1719 KTKVDPDDFFTNEQGIPPLQSLAEAKK 1799
            KTKVDPDDFFTNEQ IPPL+SLAEAKK
Sbjct: 514  KTKVDPDDFFTNEQSIPPLRSLAEAKK 540


>gb|ADY15026.1| (S)-tetrahydroprotoberberine oxidase [Berberis wilsonae]
          Length = 530

 Score =  684 bits (1765), Expect = 0.0
 Identities = 343/549 (62%), Positives = 416/549 (75%), Gaps = 4/549 (0%)
 Frame = +3

Query: 147  AIANSLVP--LASSSSEYEGFLQCLDLRSNSSIPVYNPSSTSYTSILHASIYNLRFISPT 320
            ++ +SL+P  LASS + YE FLQCLDL S +SIPVY  +++SYTSIL ++I NL F+SPT
Sbjct: 13   SLLSSLLPTSLASSDANYEDFLQCLDLYSQNSIPVYTRNTSSYTSILESTIKNLVFLSPT 72

Query: 321  TPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSSSVAPVPFVLLDLIRLRT 500
            TPKPNFI+TPM+ESHVQ +V+C R HGLQ+R RSGGHDFEG S V+ VPFV+LDLI L+T
Sbjct: 73   TPKPNFIVTPMQESHVQTSVICCRMHGLQMRIRSGGHDFEGLSYVSNVPFVVLDLIHLKT 132

Query: 501  ISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGILMRKY 680
            I+V+IE+ +AWVQTGATIGELYYRIAEK   HAFPAGLCP+VGVGGHISGAGYG+LMRKY
Sbjct: 133  INVDIEENSAWVQTGATIGELYYRIAEKVGVHAFPAGLCPTVGVGGHISGAGYGVLMRKY 192

Query: 681  GLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKXXXXXXXXXXXX 860
            G+SADHVIDAR+V+V+G ILD+ESMGED FWAIRGGGGASFGVILAWK            
Sbjct: 193  GVSADHVIDARIVNVDGEILDRESMGEDLFWAIRGGGGASFGVILAWKIRLVPVPPTVTI 252

Query: 861  XXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPEKKTIVISFLGFYLG 1040
                   E+GATAL+HKWQ I D + +DLF+GLS++++ + P     KTI++SF+G +LG
Sbjct: 253  FIVPKTLEEGATALLHKWQFIGDNVHEDLFIGLSMRSVIISP--KGDKTILVSFIGLFLG 310

Query: 1041 APEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLENDLSLLLERKNPIPPKNRY 1220
              +K +Q ME+SFPELG+   DCIEMSWIKS + FG+F  +  LS+LL+RKNP PPK+ +
Sbjct: 311  GSDKLVQHMEQSFPELGVKPHDCIEMSWIKSTVVFGVFSNDASLSVLLDRKNPFPPKSYH 370

Query: 1221 MSKSDYVHEQVSEAVLEGIWKRF--DGIDEPEIIMSPYGGKMNEIEESAIAFPHRKGNMY 1394
              KSDYV E +  +VLEGI  RF  +G+++ EIIMSPYGG+MNEI ES IAFPHRKGN+Y
Sbjct: 371  KVKSDYVTEPLPISVLEGICHRFLKNGVNKAEIIMSPYGGRMNEISESEIAFPHRKGNLY 430

Query: 1395 KINYLVSWKELGEEAEKKHASWIRELYNYMTPYVXXXXXXXXXXXXXXXXXXXXXXXXXY 1574
            KINY+  W+E G  + + H SWIRELY YMTP                           Y
Sbjct: 431  KINYIAEWEEAG--SMENHLSWIRELYRYMTP---------------------------Y 461

Query: 1575 VSKSPRCSYLNFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDPDDFFTN 1754
            VSKSPR SYLNFKD DLG  KNGTATY +AK WGSKY+KNNF RL+ VKTKVDP++FF N
Sbjct: 462  VSKSPRSSYLNFKDIDLGQTKNGTATYSQAKAWGSKYFKNNFKRLMQVKTKVDPNNFFCN 521

Query: 1755 EQGIPPLQS 1781
            EQGIPP  S
Sbjct: 522  EQGIPPFSS 530


>ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|222875303|gb|EEF12434.1|
            predicted protein [Populus trichocarpa]
          Length = 527

 Score =  574 bits (1480), Expect = e-161
 Identities = 294/555 (52%), Positives = 382/555 (68%), Gaps = 5/555 (0%)
 Frame = +3

Query: 132  FLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIP--VYNPSSTSYTSILHASIYNLR 305
            FL F+  + S V   +S+  +E FLQCLD +++ SI   +Y P ++SY+SIL  SI NLR
Sbjct: 4    FLFFLIFSFSWV---TSAHTHESFLQCLDSQNSHSISKLIYTPINSSYSSILQFSIQNLR 60

Query: 306  FISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSSSVAPVPFVLLDL 485
            F + +TPKP  I+TP   SH+QA ++CS++HG+QIR RSGGHD+EG S V+   FV++DL
Sbjct: 61   FNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVIDL 120

Query: 486  IRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVGVGGHISGAGYGI 665
            I LRTI+VN E++TAWVQ+G TIGE+YYRIAEKSRT AFPA +CP+VGVGGH SG GYG+
Sbjct: 121  INLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYGM 180

Query: 666  LMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGVILAWKXXXXXXX 845
            LMRKYGL+ADH+IDA+LVDV GRILD+ESMGED FWAIRGGGG +FGV++AWK       
Sbjct: 181  LMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSAP 240

Query: 846  XXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPDHPEKKTIVISFL 1025
                        EQ A+ LVH+WQ +ADKL +DLF+ + +  ++   +     TI  SF 
Sbjct: 241  PTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILNRVNSTEEGKSNTTIQASFN 300

Query: 1026 GFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLENDLSLLLERKNPIP 1205
              +LG  ++ L L+++SFPELGL+KEDCIEMSWI+S LYF  F   + L +LL+R  P  
Sbjct: 301  SLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSNSSLDVLLDR-TPST 359

Query: 1206 PKNRYMSKSDYVHEQVSEAVLEGIWKRF--DGIDEPEIIMSPYGGKMNEIEESAIAFPHR 1379
             +N + +KSDYV E + E  LEGIW+RF    I+ P +I SPYGGKM+EI ES+I FPHR
Sbjct: 360  RRN-FKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMSEISESSIPFPHR 418

Query: 1380 KGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVXXXXXXXXXXXXXXXXXXXXX 1559
             GN+YKI +L+ W E G  A K+H SWIR LY+Y+ P                       
Sbjct: 419  AGNIYKIQHLIYWDEEGIVATKRHISWIRRLYSYLAP----------------------- 455

Query: 1560 XXXXYVSKSPRCSYLNFKDNDLG-HKKNGTATYLEAKVWGSKYYKNNFDRLVSVKTKVDP 1736
                YVSK+PR +Y+N++D D+G +   G  +Y +A +WG KY+KNNFDRLV VKT VDP
Sbjct: 456  ----YVSKTPRAAYVNYRDLDIGINNHAGNTSYRQASIWGLKYFKNNFDRLVRVKTAVDP 511

Query: 1737 DDFFTNEQGIPPLQS 1781
             +FF NEQ IPPL S
Sbjct: 512  ANFFRNEQSIPPLSS 526


>ref|XP_002277310.1| PREDICTED: reticuline oxidase-like protein-like [Vitis vinifera]
          Length = 537

 Score =  552 bits (1422), Expect = e-154
 Identities = 283/566 (50%), Positives = 373/566 (65%), Gaps = 6/566 (1%)
 Frame = +3

Query: 102  MGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIPV----YNPSSTSY 269
            MG SS      LVF  + +S V  A+SSS ++ FLQCL L SNSS P+    Y P ++SY
Sbjct: 1    MGSSSSAETYILVFTLLFSS-VSWAASSSVHQNFLQCLTLNSNSSTPITKVLYTPKNSSY 59

Query: 270  TSILHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSS 449
             ++L  SI NLRF S  TPKP  I+TP+  SH+QA V+CS+++GLQIR RSGGHD+EG S
Sbjct: 60   ETVLDFSIQNLRFTSSCTPKPQIIVTPLHVSHIQAAVICSKKYGLQIRARSGGHDYEGLS 119

Query: 450  SVAPVPFVLLDLIRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVG 629
             V+ VPF+++DL+ LR+I+V++ED +AWV+ GAT+GE+YY IA K+  H FPAG+CP+VG
Sbjct: 120  YVSEVPFIIVDLLELRSINVDVEDGSAWVEAGATLGEVYYSIANKTAIHGFPAGICPTVG 179

Query: 630  VGGHISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGV 809
            VGGH+SG GYG L+RKYGL+AD++IDA +VD NG +L++ESMGED FWAIRGGGGASFG+
Sbjct: 180  VGGHLSGGGYGTLLRKYGLAADNIIDAYIVDSNGTLLNRESMGEDLFWAIRGGGGASFGI 239

Query: 810  ILAWKXXXXXXXXXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPD 989
            I++WK                   EQ A  ++ KWQ +ADKL +DLF+ + VQA  ++  
Sbjct: 240  IVSWKIKLVPVPSTVTVFRVTRTLEQDAEKILLKWQQVADKLHEDLFIRVYVQA--VNGS 297

Query: 990  HPEKKTIVISFLGFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLEND 1169
               ++TI  ++   +LG     L LM ESFPELGL  +DC E SWI+S LYF  F     
Sbjct: 298  QEGERTISSTYESLFLGNTSGLLSLMNESFPELGLAADDCNETSWIESVLYFAGFS-GQP 356

Query: 1170 LSLLLERKNPIPPKNRYMSKSDYVHEQVSEAVLEGIWKRFDGIDEPE--IIMSPYGGKMN 1343
            L +LL R      KN + +KSD++ E + E  L+GIWK F  +      +I+SPYGG+MN
Sbjct: 357  LDVLLNRSQ--TSKNYFKNKSDFLKEPIPETGLQGIWKLFYQVKNATALMIISPYGGRMN 414

Query: 1344 EIEESAIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVXXXXXXXXX 1523
            EI E+   FPHRKG++Y I Y+V+W E G++  K+H  W R+L+ YM P           
Sbjct: 415  EIPETETPFPHRKGSLYSIQYVVAWLEEGKKVSKRHIDWARKLHKYMAP----------- 463

Query: 1524 XXXXXXXXXXXXXXXXYVSKSPRCSYLNFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFD 1703
                            YVSKSPR +YLN++D DLG  KNG  +Y +A +WG KYYK NF+
Sbjct: 464  ----------------YVSKSPRAAYLNYRDLDLGRNKNGNTSYAQASIWGLKYYKINFN 507

Query: 1704 RLVSVKTKVDPDDFFTNEQGIPPLQS 1781
            RLV VKTKVDP +FF NEQ IPPL S
Sbjct: 508  RLVQVKTKVDPSNFFRNEQSIPPLSS 533


>ref|XP_002277294.1| PREDICTED: reticuline oxidase-like protein-like [Vitis vinifera]
          Length = 536

 Score =  551 bits (1421), Expect = e-154
 Identities = 285/562 (50%), Positives = 370/562 (65%), Gaps = 6/562 (1%)
 Frame = +3

Query: 102  MGISSLVVFPFLVFIAIANSLVPLASSSSEYEGFLQCLDLRSNSSIP----VYNPSSTSY 269
            MG SS      LVF  + +S V  A+SSS ++ FLQCL L SNSS P    VY P ++SY
Sbjct: 1    MGSSSCAETYILVFTLLFSS-VSWAASSSVHQNFLQCLTLNSNSSTPITKVVYTPHNSSY 59

Query: 270  TSILHASIYNLRFISPTTPKPNFIITPMRESHVQATVVCSREHGLQIRTRSGGHDFEGSS 449
             ++L  SI NLRF S  TP+P  I+TP+  SH+QA V+CS+++GLQIR RSGGHD+EG S
Sbjct: 60   ETVLDFSIQNLRFTSSCTPRPQVIVTPLLVSHIQAAVICSKKYGLQIRARSGGHDYEGLS 119

Query: 450  SVAPVPFVLLDLIRLRTISVNIEDETAWVQTGATIGELYYRIAEKSRTHAFPAGLCPSVG 629
             V+ VPF+++DL+ LR+I+V++ED +AWV+ GAT+GE+YY IA K+ TH FPAG+CP+VG
Sbjct: 120  YVSEVPFIIVDLLELRSINVDVEDGSAWVEAGATLGEVYYSIANKTATHGFPAGICPTVG 179

Query: 630  VGGHISGAGYGILMRKYGLSADHVIDARLVDVNGRILDKESMGEDYFWAIRGGGGASFGV 809
            VGGH+SG GYG L+RKYGL+AD++IDA +VD NG +L++ESMGED FWAIRGGGGASFG+
Sbjct: 180  VGGHLSGGGYGTLLRKYGLAADNIIDAYIVDSNGTLLNRESMGEDLFWAIRGGGGASFGI 239

Query: 810  ILAWKXXXXXXXXXXXXXXXXXXXEQGATALVHKWQIIADKLDDDLFLGLSVQALHLDPD 989
            I++WK                   EQ A  ++ KWQ +ADKL +DLF+ + VQA  ++  
Sbjct: 240  IVSWKIKLVPVPSTVTVFTVTRTLEQDAEKILLKWQQVADKLHEDLFIRVYVQA--VNGS 297

Query: 990  HPEKKTIVISFLGFYLGAPEKALQLMEESFPELGLMKEDCIEMSWIKSALYFGIFQLEND 1169
               ++TI  ++   +LG     L LM ESFPELGL  +DC E SWI+S LYF  F     
Sbjct: 298  QEGERTISSTYESLFLGNTSGLLSLMNESFPELGLAADDCNETSWIESVLYFAGFS-GQP 356

Query: 1170 LSLLLERKNPIPPKNRYMSKSDYVHEQVSEAVLEGIWKRFDGIDEPE--IIMSPYGGKMN 1343
            L +LL R      KN + +KSD++ E + E  L GIWK F  +      II+SPYGG+MN
Sbjct: 357  LDVLLNRSQ--TSKNYFKNKSDFLKEPIPETGLHGIWKLFYELKNATGMIIISPYGGRMN 414

Query: 1344 EIEESAIAFPHRKGNMYKINYLVSWKELGEEAEKKHASWIRELYNYMTPYVXXXXXXXXX 1523
            EI E+   FPHRKG++Y I Y+V+W E GEE  K+H  W R+LY YM P           
Sbjct: 415  EIPETETPFPHRKGSLYSIQYVVNWLEEGEEVSKRHIDWTRKLYKYMAP----------- 463

Query: 1524 XXXXXXXXXXXXXXXXYVSKSPRCSYLNFKDNDLGHKKNGTATYLEAKVWGSKYYKNNFD 1703
                            YVSKSPR +YLN++D DLG  KNG  +Y +A +WG KYYK NF+
Sbjct: 464  ----------------YVSKSPRAAYLNYRDLDLGRNKNGNTSYAQASIWGLKYYKINFN 507

Query: 1704 RLVSVKTKVDPDDFFTNEQGIP 1769
            RLV VKTKVDP +FF NEQ IP
Sbjct: 508  RLVQVKTKVDPSNFFRNEQSIP 529