BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00006344
         (759 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABM30178.1| steroleosin [Brassica napus]                           197   2e-48
emb|CBI15698.3| unnamed protein product [Vitis vinifera]              197   2e-48
gb|ACG69523.1| steroleosin SLO1-2 [Brassica napus]                    196   4e-48
gb|ABM90633.1| short-chain dehydrogenase reductase [Brassica nap...   195   1e-47
ref|NP_568742.1| hydroxysteroid dehydrogenase 1 [Arabidopsis tha...   193   3e-47

>gb|ABM30178.1| steroleosin [Brassica napus]
          Length = 244

 Score =  197 bits (502), Expect = 2e-48
 Identities = 94/196 (47%), Positives = 140/196 (71%)
 Frame = -3

Query: 748 FEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASVSGWLYGPNISFYAAS 569
           FE   ++T +  VMD NFWGSVY T  ALP+L++S GKI+ ++S + WL  P +SFY AS
Sbjct: 36  FENIEEITRTRAVMDTNFWGSVYTTRAALPYLRQSNGKIVAMSSSAAWLTAPRMSFYNAS 95

Query: 568 KAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKVEIDKEKRDALIGNFP 389
           KAALLNFF+TLR+E GS V +TI +PG++ESE++QGK+ + +G++ ++++ RD  IG FP
Sbjct: 96  KAALLNFFETLRIELGSDVHITIVTPGYIESELTQGKYFSGEGELVVNQDIRDVQIGAFP 155

Query: 388 VRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWFFHVLFVILPNRNKDK 209
           V S   CAK I+KGV R +R+VT+PSWF++ Y+ KV  PE++EW   +LF +  +   +K
Sbjct: 156 VTSVSGCAKGIVKGVCRKQRYVTEPSWFKVTYLWKVFCPELIEWGCRLLF-LSGHGTSEK 214

Query: 208 SSLQKSISNRNEIKKA 161
           ++L K I +   ++ A
Sbjct: 215 NALNKKILDIPGVRSA 230


>emb|CBI15698.3| unnamed protein product [Vitis vinifera]
          Length = 322

 Score =  197 bits (501), Expect = 2e-48
 Identities = 92/188 (48%), Positives = 132/188 (70%), Gaps = 1/188 (0%)
 Frame = -3

Query: 745 EESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASVSGWLYGPNISFYAASK 566
           EE+ D+T+  PVMDVNFWGSVY T  A+PHL+ S GKI+ +AS + WL  P +S Y ASK
Sbjct: 116 EEATDITNFRPVMDVNFWGSVYTTRFAIPHLRNSHGKIIGMASSAAWLPMPRMSIYNASK 175

Query: 565 AALLNFFDTLRVEFGSSVK-VTIASPGFVESEMSQGKHRTMQGKVEIDKEKRDALIGNFP 389
           AAL++F+DTLR+E GS +  +T+ +PGF+ESEM+QG+    + ++E+D++ RDA +   P
Sbjct: 176 AALISFYDTLRIELGSDIGGITVVTPGFIESEMTQGEFLKNEDQIEVDQDMRDAQVSIIP 235

Query: 388 VRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWFFHVLFVILPNRNKDK 209
           V  A  CAK I+K   RGE+++T+PSWFR+ Y+ KVL PEV+EW F + ++  P  ++  
Sbjct: 236 VGRAEACAKAIVKSACRGEKYLTEPSWFRVTYLWKVLCPEVIEWCFRLFYLARPGESETL 295

Query: 208 SSLQKSIS 185
           S     IS
Sbjct: 296 SKKILDIS 303


>gb|ACG69523.1| steroleosin SLO1-2 [Brassica napus]
          Length = 349

 Score =  196 bits (498), Expect = 4e-48
 Identities = 93/196 (47%), Positives = 140/196 (71%)
 Frame = -3

Query: 748 FEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASVSGWLYGPNISFYAAS 569
           FE   ++T +  VMD NFWGSVY T  ALP+L++S GKI+ ++S + WL  P +SFY AS
Sbjct: 141 FENIEEITRTRAVMDTNFWGSVYTTRAALPYLRQSNGKIVAMSSSAAWLTAPRMSFYNAS 200

Query: 568 KAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKVEIDKEKRDALIGNFP 389
           KAALLNFF+TLR+E GS V +TI +PG++ESE++QGK+ + +G++ ++++ RD  IG FP
Sbjct: 201 KAALLNFFETLRIELGSDVHITIVTPGYIESELTQGKYFSGEGELVVNQDIRDVQIGAFP 260

Query: 388 VRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWFFHVLFVILPNRNKDK 209
           V S   CAK I+KGV R +R+VT+PSWF++ Y+ KV  PE++EW   +LF +  +   ++
Sbjct: 261 VTSVSGCAKGIVKGVCRKQRYVTEPSWFKVTYLWKVFCPELIEWGCRLLF-LSGHGTSEE 319

Query: 208 SSLQKSISNRNEIKKA 161
           ++L K I +   ++ A
Sbjct: 320 NALNKKILDIPGVRSA 335


>gb|ABM90633.1| short-chain dehydrogenase reductase [Brassica napus]
           gi|321173854|gb|ADW77633.1| steroleosin-A [Brassica
           napus] gi|321173856|gb|ADW77634.1| steroleosin-A
           [Brassica napus]
          Length = 349

 Score =  195 bits (495), Expect = 1e-47
 Identities = 92/196 (46%), Positives = 140/196 (71%)
 Frame = -3

Query: 748 FEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASVSGWLYGPNISFYAAS 569
           FE   ++T +  VMD NFWG+VY T  ALP+L++S GKI+ ++S + WL  P +SFY AS
Sbjct: 141 FENIEEITRTRAVMDTNFWGAVYTTRAALPYLRQSNGKIVAMSSSAAWLTAPRMSFYNAS 200

Query: 568 KAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKVEIDKEKRDALIGNFP 389
           KAALLNFF+TLR+E GS V +TI +PG++ESE++QGK+ + +G++ ++++ RD  IG FP
Sbjct: 201 KAALLNFFETLRIELGSDVHITIVTPGYIESELTQGKYVSGEGELVVNQDIRDVQIGAFP 260

Query: 388 VRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWFFHVLFVILPNRNKDK 209
           V S   CAK I+KGV R ER+VT+PSWF++ Y+ KV  PE++EW   ++F +  +   ++
Sbjct: 261 VTSVSGCAKGIVKGVCRKERYVTEPSWFKVTYLWKVFCPELIEWGCRLMF-LSGHGTPEE 319

Query: 208 SSLQKSISNRNEIKKA 161
           ++L K I +   ++ A
Sbjct: 320 NALNKKILDIPGVRSA 335


>ref|NP_568742.1| hydroxysteroid dehydrogenase 1 [Arabidopsis thaliana]
           gi|22327700|ref|NP_680418.1| hydroxysteroid
           dehydrogenase 1 [Arabidopsis thaliana]
           gi|17386150|gb|AAL38621.1|AF446888_1 AT5g50700/MFB16_9
           [Arabidopsis thaliana] gi|8777393|dbj|BAA96983.1|
           11-beta-hydroxysteroid dehydrogenase-like [Arabidopsis
           thaliana] gi|9758769|dbj|BAB09145.1|
           11-beta-hydroxysteroid dehydrogenase-like [Arabidopsis
           thaliana] gi|15450585|gb|AAK96564.1| AT5g50700/MFB16_9
           [Arabidopsis thaliana] gi|17065384|gb|AAL32846.1|
           11-beta-hydroxysteroid dehydrogenase-like [Arabidopsis
           thaliana] gi|20148649|gb|AAM10215.1|
           11-beta-hydroxysteroid dehydrogenase-like [Arabidopsis
           thaliana] gi|62320743|dbj|BAD95411.1|
           11-beta-hydroxysteroid dehydrogenase-like [Arabidopsis
           thaliana] gi|332008584|gb|AED95967.1| hydroxysteroid
           dehydrogenase 1 [Arabidopsis thaliana]
           gi|332008598|gb|AED95981.1| hydroxysteroid dehydrogenase
           1 [Arabidopsis thaliana]
          Length = 349

 Score =  193 bits (491), Expect = 3e-47
 Identities = 89/187 (47%), Positives = 137/187 (73%)
 Frame = -3

Query: 748 FEESADVTSSLPVMDVNFWGSVYPTHMALPHLKKSKGKILVIASVSGWLYGPNISFYAAS 569
           FE   D+T +  V+D NFWGSVY T  ALP+L++S GKI+ ++S + WL  P +SFY AS
Sbjct: 141 FENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIVAMSSSAAWLTAPRMSFYNAS 200

Query: 568 KAALLNFFDTLRVEFGSSVKVTIASPGFVESEMSQGKHRTMQGKVEIDKEKRDALIGNFP 389
           KAALL+FF+T+R+E G  V +TI +PG++ESE++QGK+ + +G++ ++++ RD  +G FP
Sbjct: 201 KAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFSGEGELIVNQDMRDVQVGPFP 260

Query: 388 VRSARECAKTILKGVRRGERHVTDPSWFRILYVHKVLVPEVVEWFFHVLFVILPNRNKDK 209
           V SA  CAK+I+ GV R +R+VT+PSWF++ Y+ KVL PE++EW   +L++     ++D 
Sbjct: 261 VASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPELIEWGCRLLYMTGTGMSED- 319

Query: 208 SSLQKSI 188
           ++L K I
Sbjct: 320 TALNKRI 326