BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00005572
         (2196 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   503   e-139
ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   459   e-126
ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|2...   451   e-124
ref|XP_004136614.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   422   e-115
ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabid...   416   e-113

>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            gi|297739656|emb|CBI29838.3| unnamed protein product
            [Vitis vinifera]
          Length = 468

 Score =  503 bits (1294), Expect = e-139
 Identities = 274/464 (59%), Positives = 331/464 (71%), Gaps = 32/464 (6%)
 Frame = +2

Query: 284  AGACGSGCYKAXXXXXXXXXXN-TGRT---------------CYKCKVAKIIESTGGL-- 409
            AG C SGC++             T RT               C KCKV + + S+G +  
Sbjct: 5    AGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASSGTVSV 64

Query: 410  -------CGECLDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKA 568
                   C +C   +LF KFK AV++  +ISPTDNVL+AFSGGP SRVAL+FVHEMQ+KA
Sbjct: 65   DGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRVALQFVHEMQHKA 124

Query: 569  QKNRDASRNKSYPVFGVGVAFIDETAVTSL---QLDKAIQDLRMIVSSLAPPRKELHIMP 739
            QKN DASR++S PVFGVGVAF+DE+AV+ +   + DKAIQD++ IVS+LAPP K  H++P
Sbjct: 125  QKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSNLAPPDKAFHVVP 184

Query: 740  IQTILSDDDDVNRLKELLLTVTDVTGKEDLLLHLRMLCLQKVALENNYNKLILGSCTSRI 919
            I+++ S +   +RL+ELL  V DVTGKEDLL HLRML LQK A EN Y KL+LGSCTSRI
Sbjct: 185  IESVYSSNGR-DRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYENGYTKLLLGSCTSRI 243

Query: 920  ACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQELNMLCRLNSLKTLDSFDS 1099
            ACHV+SATVKGQGYSLPADIQY DARWEIPVVLPLRDC++QELNMLCRL+ LKT++  +S
Sbjct: 244  ACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLAQELNMLCRLDGLKTMEVLNS 303

Query: 1100 PRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNRINESLDS----GDRRRRRK 1267
            P SGIN LV++F+TLL EENP+RE TIVRTA KL PF FN+I ES DS      RRR++K
Sbjct: 304  PHSGINGLVASFVTLLQEENPSRECTIVRTAGKLTPFHFNQIPESDDSNVHLASRRRQKK 363

Query: 1268 VNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSPTVFGESCCPSCQFQILPKE 1447
             NLK  ES+  E  CPIC SP++  D   L S L S  T+  +FG +CCPSCQFQILPKE
Sbjct: 364  FNLKPNESVPPESYCPICNSPLSKSDHINL-SSLGSDQTNADIFGATCCPSCQFQILPKE 422

Query: 1448 SSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSDGED 1579
              SMEHFYSL+PQQ + + KD     Q  LREQI+D LLSD ED
Sbjct: 423  PVSMEHFYSLLPQQFVVQAKDRSFSMQRQLREQIQDFLLSDSED 466


>ref|XP_003521174.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Glycine max]
          Length = 458

 Score =  459 bits (1181), Expect = e-126
 Identities = 244/454 (53%), Positives = 317/454 (69%), Gaps = 21/454 (4%)
 Frame = +2

Query: 284  AGACGSGCYK-------AXXXXXXXXXXNTGRTCYKCKVAKIIESTGGL-----CGECLD 427
            +G    GCYK                  N    C KCK+   +   GG+     C +C  
Sbjct: 6    SGCQSGGCYKDEDATCKQPPKSEEADSKNPSNVCIKCKLNDAVSGYGGIDDGRFCADCFK 65

Query: 428  RSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKAQKNRDASRNKSYP 607
             +LF KF+ AV++  +I+PTD VL+AFSGGP SRVAL+FVH+MQ +AQ+N DASR++S P
Sbjct: 66   TNLFGKFRFAVTSNAMITPTDKVLVAFSGGPSSRVALQFVHDMQERAQRNFDASRDRSLP 125

Query: 608  VFGVGVAFIDETAVTSL---QLDKAIQDLRMIVSSLAPPRKELHIMPIQTILSDD--DDV 772
            VFGVGV FIDE+AV  +   ++++A++ +  +VSSLAPPRKELHI+PI+T+ S D  D  
Sbjct: 126  VFGVGVVFIDESAVLPIPSNEMEEAVEVVSEVVSSLAPPRKELHIVPIETVYSSDSGDGK 185

Query: 773  NRLKELLLTVTDVTGKEDLLLHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATVKG 952
             R+ +++ TV D TG+ED+LL LRML LQKVA E  YN++ILGSC SRIACHV+SATVKG
Sbjct: 186  ERITKVVNTVIDPTGREDVLLCLRMLALQKVASEFGYNRIILGSCISRIACHVISATVKG 245

Query: 953  QGYSLPADIQYADARWEIPVVLPLRDCVSQELNMLCRLNSLKTLDSFDSPRSGINELVST 1132
            QGYSLPADIQY DARWE+PVVLPLRDC +QE+NMLC L+ LKT+       S IN LVS+
Sbjct: 246  QGYSLPADIQYVDARWEVPVVLPLRDCFAQEINMLCHLDGLKTVKLSTDLCSSINGLVSS 305

Query: 1133 FITLLMEENPARERTIVRTAEKLVPFQFNRINESLDS----GDRRRRRKVNLKSYESISS 1300
            F+ LL EENP+RE TIVRTA KL PFQFNRI E +D       RRR+++ NLKS ES+SS
Sbjct: 306  FVALLQEENPSRESTIVRTAGKLTPFQFNRIPEIIDGNVPLATRRRQKRYNLKSNESVSS 365

Query: 1301 EFLCPICCSPVNNLDLEYLKSKLESCATSPTVFGESCCPSCQFQILPKESSSMEHFYSLV 1480
            E  CP+C SP++  ++    S ++S  +S  +F  +CC SC+FQILP +S +ME FY  +
Sbjct: 366  ESFCPLCNSPLDKSEITDW-SNIDSHRSS-DIFYNTCCSSCRFQILPSDSMAMEQFYMDL 423

Query: 1481 PQQMITRVKDSVGGDQAWLREQIEDCLLSDGEDE 1582
            PQ ++ R K +  G+ + LREQI+D LLSDGEDE
Sbjct: 424  PQSVVGRAKQANNGNLSLLREQIQDYLLSDGEDE 457


>ref|XP_002308030.1| predicted protein [Populus trichocarpa] gi|222854006|gb|EEE91553.1|
            predicted protein [Populus trichocarpa]
          Length = 443

 Score =  451 bits (1159), Expect = e-124
 Identities = 244/431 (56%), Positives = 306/431 (70%), Gaps = 23/431 (5%)
 Frame = +2

Query: 284  AGACGSGCYK--------AXXXXXXXXXXNTGRTCYKCKVAKIIE--STGG----LCGEC 421
            +  C S CYK                   N+   C KCK    I   S  G     C +C
Sbjct: 5    SSGCQSNCYKNGDQVSDSPPPPPTLAAANNSNNLCLKCKTNSPITPGSLSGDEARFCADC 64

Query: 422  LDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKAQKNRDASRNKS 601
               +++ KF+ AV++Q +ISP+DNVL+AFSGGP SRVAL+FVH +Q++AQKN +AS++KS
Sbjct: 65   FRSNIYGKFRLAVTSQAMISPSDNVLVAFSGGPSSRVALQFVHGLQDRAQKNFEASKDKS 124

Query: 602  YPVFGVGVAFIDETAVTSLQLDK---AIQDLRMIVSSLAPPRKELHIMPIQTILSDD--D 766
             PVFGVGVAFIDE+AV  +  DK   AIQD+R +VS+LAPP K+LH++PI+ I + D  D
Sbjct: 125  LPVFGVGVAFIDESAVYPVSSDKVDGAIQDMRSVVSNLAPPTKQLHVVPIENIYASDSSD 184

Query: 767  DVNRLKELLLTVTDVTGKEDLLLHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATV 946
              +RL +LL  V D TGKEDLLLHLRML LQ VA +N YN+LILGSCTSRIACHVL+ATV
Sbjct: 185  GKDRLTKLLDAVNDATGKEDLLLHLRMLALQMVASQNGYNRLILGSCTSRIACHVLAATV 244

Query: 947  KGQGYSLPADIQYADARWEIPVVLPLRDCVSQELNMLCRLNSLKTLDSFDSPRSGINELV 1126
            KGQGYSL ADIQY DARWEIPVVLPLRDC SQELN+LC+L+ LKTL  FDS  SGIN L+
Sbjct: 245  KGQGYSLSADIQYVDARWEIPVVLPLRDCTSQELNLLCQLDGLKTLQPFDSTPSGINGLI 304

Query: 1127 STFITLLMEENPARERTIVRTAEKLVPFQFNRINE----SLDSGDRRRRRKVNLKSYESI 1294
            S+F+ +L EEN +RE TI+RTA KL PF+FNRI E    ++    RRR+++ NLK+ ESI
Sbjct: 305  SSFVNVLQEENSSRECTILRTAGKLTPFEFNRIPETDNCNVPLAIRRRQKRYNLKTKESI 364

Query: 1295 SSEFLCPICCSPVNNLDLEYLKSKLESCATSPTVFGESCCPSCQFQILPKESSSMEHFYS 1474
            SSE  CPIC +P+ N +   L S L+SC +S   F  +CC SCQFQI+PK+ SSM +F S
Sbjct: 365  SSELFCPICNAPLKNFNSLNL-SSLKSCQSSR--FNAACCSSCQFQIIPKDPSSMGNFCS 421

Query: 1475 LVPQQMITRVK 1507
            L+PQQ++ R K
Sbjct: 422  LLPQQVVDRAK 432


>ref|XP_004136614.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Cucumis
            sativus]
          Length = 469

 Score =  422 bits (1086), Expect = e-115
 Identities = 224/422 (53%), Positives = 301/422 (71%), Gaps = 15/422 (3%)
 Frame = +2

Query: 362  CYKCKVAKIIESTG-----GLCGECLDRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCS 526
            C+KCK  + + ++G       C +C   +LFAKF+QAV++  +ISP+D VL+AFSGGPCS
Sbjct: 55   CFKCKSKERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCS 114

Query: 527  RVALEFVHEMQNKAQKNRDASRNKSYPVFGVGVAFIDETAVTSL---QLDKAIQDLRMIV 697
            RVAL+FVHEMQ KA  N +ASR++S  VFGVGVA+I+ETA  S+   ++DK +Q ++ +V
Sbjct: 115  RVALQFVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVV 174

Query: 698  SSLAPPRKELHIMPIQTILSDD--DDVNRLKELLLTVTDVTGKEDLLLHLRMLCLQKVAL 871
            SSL  PRK LHI+ I+++ S +  D   +LK+LL  V DVTGKEDL+ +LRML LQK+A 
Sbjct: 175  SSLCQPRKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAA 234

Query: 872  ENNYNKLILGSCTSRIACHVLSATVKGQGYSLPADIQYADARWEIPVVLPLRDCVSQELN 1051
            EN YN+L++G+CTSRIACHV+S+TVKGQGYSLP D+QY DARWE+PVVLPL DC++QELN
Sbjct: 235  ENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELN 294

Query: 1052 MLCRLNSLKTLDSFDSPRSGINELVSTFITLLMEENPARERTIVRTAEKLVPFQFNRINE 1231
            MLC ++ LKT++      SGIN+LVS+F+ LL  ENP+RE TIVRTA KL PF F+RI +
Sbjct: 295  MLCCVDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPD 354

Query: 1232 SLDSGD-----RRRRRKVNLKSYESISSEFLCPICCSPVNNLDLEYLKSKLESCATSPTV 1396
              +S +     +RR+++ N K   S+SSE  C +C  P+N  D+      L   A S ++
Sbjct: 355  LNESNNVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDV------LTRHAGS-SI 407

Query: 1397 FGESCCPSCQFQILPKESSSMEHFYSLVPQQMITRVKDSVGGDQAWLREQIEDCLLSDGE 1576
             G +CC SCQFQILPK+SSS E F SL+P  M+ R      G+   LR++I++ LLSD E
Sbjct: 408  LG-ACCSSCQFQILPKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDE 466

Query: 1577 DE 1582
             E
Sbjct: 467  AE 468


>ref|NP_567993.1| cytoplasmic tRNA 2-thiolation protein 2 [Arabidopsis thaliana]
            gi|332278212|sp|O65628.3|CTU2_ARATH RecName:
            Full=Cytoplasmic tRNA 2-thiolation protein 2
            gi|115646788|gb|ABJ17118.1| At4g35910 [Arabidopsis
            thaliana] gi|332661188|gb|AEE86588.1| cytoplasmic tRNA
            2-thiolation protein 2 [Arabidopsis thaliana]
          Length = 458

 Score =  416 bits (1069), Expect = e-113
 Identities = 230/456 (50%), Positives = 303/456 (66%), Gaps = 22/456 (4%)
 Frame = +2

Query: 284  AGACGSGCY-------KAXXXXXXXXXXNTGRTCYKCKV-AKIIESTGGL-----CGECL 424
            +  C SGCY                   N    C KCK  A +    GG      C +C 
Sbjct: 5    SSGCESGCYDREKDNGSKIVDDAVSGGGNHESVCVKCKCNAPMTFGDGGFDDGRFCADCF 64

Query: 425  DRSLFAKFKQAVSTQNLISPTDNVLLAFSGGPCSRVALEFVHEMQNKAQKNRDASRNKSY 604
              ++F KF+ AV++  +I+P+DNVL+AFSGG  SRV+L+FVHE+Q KA KN +ASR++S 
Sbjct: 65   RNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQFVHELQIKALKNYEASRDRSL 124

Query: 605  PVFGVGVAFIDETAV---TSLQLDKAIQDLRMIVSSLAPPRKELHIMPIQTILSDD--DD 769
            PVFGVGVAF+DETA     S ++  AI+ +R  VS L+PP K+LH++P+++I   D  D 
Sbjct: 125  PVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSPPAKDLHVVPVESIFGSDSLDA 184

Query: 770  VNRLKELLLTVTDVTGKEDLLLHLRMLCLQKVALENNYNKLILGSCTSRIACHVLSATVK 949
             +RL +LL +V D TGKEDLLLHL+ML LQKVA EN YN+L+LGSCTSRIA HVL+ATVK
Sbjct: 185  RDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYNRLVLGSCTSRIASHVLTATVK 244

Query: 950  GQGYSLPADIQYADARWEIPVVLPLRDCVSQELNMLCRLNSLKTLDSFDSPRSGINELVS 1129
            G+GYSL ADIQ+ DARW++P+VLPLRDCV  E+  LC L+ LKT++     + GIN+LVS
Sbjct: 245  GRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLLDGLKTVELACRSQCGINDLVS 304

Query: 1130 TFITLLMEENPARERTIVRTAEKLVPFQFNRINESLDS----GDRRRRRKVNLKSYESIS 1297
            +F+ LL EENP+RE TIVRTA KL PF FN+I E+ DS      +RR ++ NLK   S++
Sbjct: 305  SFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDSNVPMATQRRLKRFNLKYDGSMT 364

Query: 1298 SEFLCPICCSPVNNLDLEYLKSKLESCATSPTVFGESCCPSCQFQILPKESSSMEHFYSL 1477
            +E  CPIC  P+N  D   L +  E       V   +CC SC+FQILP++ SS+E F S 
Sbjct: 365  TEAFCPICNGPLNRSDSSELDTFEE--GQESDVLYAACCSSCRFQILPQDGSSLEQFSSF 422

Query: 1478 VPQQMITRVKDSVGGDQAWLREQIEDCLLSDGEDEV 1585
            +P  MI++VK      QA+LRE+I+DCLL D E+ V
Sbjct: 423  LPDHMISQVKHQKVDSQAYLREKIKDCLLLDDEEVV 458