BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00005340
         (1092 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin...   393   e-107
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi...   361   2e-97
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin...   358   2e-96
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro...   347   2e-93
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      328   1e-87

>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
            Short=EcTNMT gi|226897702|gb|ACO90222.1|
            tetrahydroprotoberberine N-methyltransferase
            [Eschscholzia californica]
          Length = 350

 Score =  393 bits (1010), Expect = e-107
 Identities = 177/263 (67%), Positives = 227/263 (86%)
 Frame = -2

Query: 1091 GQTLKQSFCYFEDESTTLDEAEAATHDLYCERAQIQDGQKILDIGCGQGSFVIHVAQKFK 912
            G+T+KQS CYF+DE++T+DEAE A H+LYCERAQI+DGQ +LDIGCGQG  V+++A+K+K
Sbjct: 85   GKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAEKYK 144

Query: 911  GCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMKNV 732
             CHVTG+TNS+AQ + IE++ + LEL+NVDVI ADVT +DT +T+DRI+V+E +EHMKN+
Sbjct: 145  NCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKTYDRILVVETIEHMKNI 204

Query: 731  GLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEEDWYSNYIFPKGSVTIPGASTLLY 552
             LF++K+S WMTED LLF+D+I HKTF H+FEA+DE+DWYS +IFPKG VTI  +STLLY
Sbjct: 205  QLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDWYSGFIFPKGCVTILSSSTLLY 264

Query: 551  FQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAARKILEPCLGSKDAVDKVITHSRTF 372
            FQDDV+ +D WV NG HMAR+VE W  KLD+ I+AAR+ILEP LGSK+AV++VITH RTF
Sbjct: 265  FQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGLGSKEAVNQVITHIRTF 324

Query: 371  CIGAYEQFSFNDGEEYMITHILF 303
            CIG YEQFS+N+GEE+MIT ILF
Sbjct: 325  CIGGYEQFSYNNGEEWMITQILF 347


>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
            Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
            tetrahydroprotoberberine N-methyltransferase [Papaver
            bracteatum]
          Length = 358

 Score =  361 bits (927), Expect = 2e-97
 Identities = 162/264 (61%), Positives = 218/264 (82%), Gaps = 1/264 (0%)
 Frame = -2

Query: 1091 GQTLKQSFCYFEDESTTLDEAEAATHDLYCERAQIQDGQKILDIGCGQGSFVIHVAQKFK 912
            G+T+KQS CYF+ ES T+DEAE A H+LYCERAQI+DGQ +LDIGCGQG  V+++A+K+K
Sbjct: 92   GKTVKQSMCYFKHESATIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIARKYK 151

Query: 911  GCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMKNV 732
             CHVTG+TNS+AQ + + ++ + L L+NVD ILADVT Y++ +T+DR+++IEA+EHMKN+
Sbjct: 152  KCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNL 211

Query: 731  GLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEEDWYSNYIFPKGSVTIPGASTLLY 552
             LF++K+S WMTE+ LLF+D++CHKTF H FEA+DE+DWYS +IFP G  TI  A++LLY
Sbjct: 212  QLFMKKLSTWMTEESLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLY 271

Query: 551  FQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAARKILEPCL-GSKDAVDKVITHSRT 375
            FQDDV+VVD WV NG HMAR+V+ W   LDKN++AA++IL P L GS +AV+ V+TH RT
Sbjct: 272  FQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHEAVNGVVTHIRT 331

Query: 374  FCIGAYEQFSFNDGEEYMITHILF 303
            FC+G YEQFS NDG+E+M+  +LF
Sbjct: 332  FCMGGYEQFSMNDGDEWMVAQLLF 355


>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
            Short=PsTNMT gi|67764091|gb|AAY79177.1|
            S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-
            N-methyltransferase [Papaver somniferum]
          Length = 358

 Score =  358 bits (918), Expect = 2e-96
 Identities = 160/264 (60%), Positives = 216/264 (81%), Gaps = 1/264 (0%)
 Frame = -2

Query: 1091 GQTLKQSFCYFEDESTTLDEAEAATHDLYCERAQIQDGQKILDIGCGQGSFVIHVAQKFK 912
            G+T+KQS CYF  ES T+DEAE A H+LYCERAQI+DGQ +LDIGCGQG  V+++AQK+K
Sbjct: 92   GKTVKQSMCYFTHESATIDEAEEAAHELYCERAQIKDGQTVLDIGCGQGGLVLYIAQKYK 151

Query: 911  GCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMKNV 732
             CHVTG+TNS+AQ + + ++ + L L+NVD ILADVT Y++ +T+DR+++IEA+EHMKN+
Sbjct: 152  NCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMKNL 211

Query: 731  GLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEEDWYSNYIFPKGSVTIPGASTLLY 552
             LF++K+S WMT++ LLF+D++CHKTF H FEA+DE+DWYS +IFP G  TI  A++LLY
Sbjct: 212  QLFMKKLSTWMTKESLLFVDHVCHKTFAHFFEAVDEDDWYSGFIFPPGCATILAANSLLY 271

Query: 551  FQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAARKILEPCL-GSKDAVDKVITHSRT 375
            FQDDV+VVD WV NG HMAR+V+ W   LDKN++AA++IL P L GS + V+ V+TH RT
Sbjct: 272  FQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAAKEILLPGLGGSHETVNGVVTHIRT 331

Query: 374  FCIGAYEQFSFNDGEEYMITHILF 303
            FC+G YEQFS N+G+E+M+  +LF
Sbjct: 332  FCMGGYEQFSMNNGDEWMVAQLLF 355


>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
            N-methyltransferase 2; Short=PbTNMT2
            gi|226897730|gb|ACO90236.1| putative N-methyltransferase
            [Papaver bracteatum]
          Length = 358

 Score =  347 bits (891), Expect = 2e-93
 Identities = 162/264 (61%), Positives = 208/264 (78%), Gaps = 1/264 (0%)
 Frame = -2

Query: 1091 GQTLKQSFCYFEDESTTLDEAEAATHDLYCERAQIQDGQKILDIGCGQGSFVIHVAQKFK 912
            G+++K S CYF+ ES+T+DEAE A+H+LYCERAQI+DGQ +LDIGCGQG  V++VAQK+K
Sbjct: 92   GKSMKLSGCYFKHESSTIDEAEEASHELYCERAQIKDGQTVLDIGCGQGGLVLYVAQKYK 151

Query: 911  GCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMKNV 732
             CHVTG+TNS+ Q + I ++ + L L NVDVILADVT Y++ +T+DRI+VI  VEHMKN+
Sbjct: 152  NCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQYESDKTYDRILVIGVVEHMKNM 211

Query: 731  GLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEEDWYSNYIFPKGSVTIPGASTLLY 552
             LF++K+S WM ED LLF+D+ CHKTF H FEA+DE+DWYS YIFP G  T   A +LLY
Sbjct: 212  QLFIKKLSTWMAEDSLLFVDHSCHKTFNHFFEALDEDDWYSGYIFPPGCATFLSADSLLY 271

Query: 551  FQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAARKILEPCL-GSKDAVDKVITHSRT 375
            FQDDV+VVD WV NG H ARTV+ W  KLDKN++A ++IL P L G+ +AV+ VITH RT
Sbjct: 272  FQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAVKEILLPGLGGNHEAVNGVITHIRT 331

Query: 374  FCIGAYEQFSFNDGEEYMITHILF 303
             C+G Y QFS NDG+E+M   +LF
Sbjct: 332  CCVGGYVQFSLNDGDEWMNAQLLF 355


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  328 bits (842), Expect = 1e-87
 Identities = 146/263 (55%), Positives = 199/263 (75%)
 Frame = -2

Query: 1091 GQTLKQSFCYFEDESTTLDEAEAATHDLYCERAQIQDGQKILDIGCGQGSFVIHVAQKFK 912
            G  LK S CYF+++STTLDEAE A  DLYCERAQIQDGQ +LD+GCGQG+  +HVAQK+K
Sbjct: 93   GSNLKGSCCYFKEDSTTLDEAEIAMLDLYCERAQIQDGQSVLDLGCGQGALTLHVAQKYK 152

Query: 911  GCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMKNV 732
             C VT +TNS +QK  IEE  +   L NV+V LAD+T ++  ET+DRI+VIE  EHMKN 
Sbjct: 153  NCRVTAVTNSVSQKEYIEEESRRRNLLNVEVKLADITTHEMAETYDRILVIELFEHMKNY 212

Query: 731  GLFLRKISKWMTEDGLLFLDYICHKTFGHHFEAIDEEDWYSNYIFPKGSVTIPGASTLLY 552
             L LRKIS+W+++DGLLFL++ICHKTF +H+E +D++DW++ Y+FP G++ IP AS  LY
Sbjct: 213  ELLLRKISEWISKDGLLFLEHICHKTFAYHYEPLDDDDWFTEYVFPAGTMIIPSASFFLY 272

Query: 551  FQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAARKILEPCLGSKDAVDKVITHSRTF 372
            FQDDV+VV+ W  +GKH +RT EEWL +LD N+D  + + E  +G+++   K+I + R F
Sbjct: 273  FQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANLDVIKPMFETLMGNEEEAVKLINYWRGF 332

Query: 371  CIGAYEQFSFNDGEEYMITHILF 303
            C+   E F +N+GEE+M +H+LF
Sbjct: 333  CLSGMEMFGYNNGEEWMASHVLF 355