BLASTX nr result
ID: Coptis24_contig00005301
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00005301
(1854 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002333242.1| predicted protein [Populus trichocarpa] gi|2... 550 0.0
ref|XP_002302861.1| predicted protein [Populus trichocarpa] gi|2... 545 0.0
ref|XP_002274828.1| PREDICTED: glucan endo-1,3-beta-glucosidase ... 544 0.0
ref|XP_004140121.1| PREDICTED: glucan endo-1,3-beta-glucosidase ... 540 0.0
ref|XP_002525335.1| Glucan endo-1,3-beta-glucosidase precursor, ... 538 0.0
>ref|XP_002333242.1| predicted protein [Populus trichocarpa]
gi|224129034|ref|XP_002320484.1| predicted protein
[Populus trichocarpa] gi|118480997|gb|ABK92452.1| unknown
[Populus trichocarpa] gi|222835778|gb|EEE74213.1|
predicted protein [Populus trichocarpa]
gi|222861257|gb|EEE98799.1| predicted protein [Populus
trichocarpa]
Length = 470
Score = 550 bits (1418), Expect(2) = 0.0
Identities = 272/367 (74%), Positives = 314/367 (85%), Gaps = 3/367 (0%)
Frame = -1
Query: 1701 LTIFLCFISIFILADAGQIGVNYGRVANNLPSAVKVVQLLKSQGIERVKLYDTDPSVLKA 1522
L FL + + ADAG +GVNYGR+ANNLPSAVKVV L+KSQG+ERVK+YDTDP+VLKA
Sbjct: 6 LPCFLLLLCVVAFADAGSVGVNYGRIANNLPSAVKVVNLVKSQGLERVKVYDTDPAVLKA 65
Query: 1521 LAGSNIEVTVALPNELLYSVSKTKSLAFKWVQKNILSYYPSTQIHAIAVGNEVFADPNNT 1342
L+GS I+VTV LPN+LLYS +K + A WVQKNI++Y+PSTQI +IAVGNEVF DP+NT
Sbjct: 66 LSGSGIKVTVDLPNQLLYSAAKYPNFARSWVQKNIVAYHPSTQIESIAVGNEVFVDPHNT 125
Query: 1341 TNFLVPAMQNIHSALFKYNLHSAIKVSSPIALSALQSSYPSSAGSFKPVLIEPVIKPMLG 1162
T FL+PAM+NIH AL K+NLHS+IKVSSPIALSALQSSYPSSAGSF+P LIEPV KPML
Sbjct: 126 TKFLIPAMKNIHQALVKFNLHSSIKVSSPIALSALQSSYPSSAGSFRPELIEPVFKPMLD 185
Query: 1161 FLRQTGSYLMVNAYPFFAYEGNTDVISLDYALFRENPGVVDSGNGLRYFALFDAQVDAVF 982
FLRQTGSYLMVNAYPFFAYE N+DVISLDYALFRENPGVVDSGNGL+YF LFDAQ+DAVF
Sbjct: 186 FLRQTGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDSGNGLKYFNLFDAQIDAVF 245
Query: 981 AAMSALKFEDLKMVVTETGWPSKGDGIERGASEENAAAYNGNLVRRVINGGGTPLRPNEN 802
AA+SALK++D+KMVVTETGWPSKGD E GAS ENAAAYNGNLVRR++ GGGTPL+P +
Sbjct: 246 AALSALKYDDVKMVVTETGWPSKGDENEVGASVENAAAYNGNLVRRILTGGGTPLKPQAD 305
Query: 801 LEVYLFALFNENKKPGPTSERNYGLFYPNEEKVYEIPFTPAQLKKWKS---KPVNRGGSE 631
L VYLFALFNEN+K GPTSERNYGLFYP+++KVY+IPFT LK +K+ PV+ G
Sbjct: 306 LTVYLFALFNENEKDGPTSERNYGLFYPDQQKVYDIPFTVEGLKNYKAPSRSPVSGGQQV 365
Query: 630 KGPVNKG 610
PV G
Sbjct: 366 SAPVRGG 372
Score = 160 bits (404), Expect(2) = 0.0
Identities = 71/100 (71%), Positives = 82/100 (82%)
Frame = -3
Query: 496 KGASTRNMSGGTWCVANEKIRKEKLQAALDFACGEGGADCRQIQPGSTCYDPNTVEAHAS 317
+G +++ +G TWCVAN KEKLQAALDFACGEGGADCR IQP +TCY PNT+ AH+S
Sbjct: 370 RGGVSKSTTGNTWCVANPDAGKEKLQAALDFACGEGGADCRPIQPDATCYSPNTLVAHSS 429
Query: 316 FAFNSYYQRNGRAMGSCYFGGAAFVVTQLPKYGNCKFTTG 197
FAFNSYYQ+ GR MG CYFGGAAFVVTQ PK+G C+F TG
Sbjct: 430 FAFNSYYQKKGRGMGDCYFGGAAFVVTQEPKFGVCEFPTG 469
>ref|XP_002302861.1| predicted protein [Populus trichocarpa] gi|222844587|gb|EEE82134.1|
predicted protein [Populus trichocarpa]
Length = 470
Score = 545 bits (1403), Expect(2) = 0.0
Identities = 271/367 (73%), Positives = 313/367 (85%), Gaps = 3/367 (0%)
Frame = -1
Query: 1701 LTIFLCFISIFILADAGQIGVNYGRVANNLPSAVKVVQLLKSQGIERVKLYDTDPSVLKA 1522
L+ FL + IF ADAG IGVNYGR+ANNLP+A KVVQL+KSQG+ER+K+YDTDP VLKA
Sbjct: 6 LSSFLVLVCIFTSADAGSIGVNYGRIANNLPAAAKVVQLVKSQGLERIKVYDTDPIVLKA 65
Query: 1521 LAGSNIEVTVALPNELLYSVSKTKSLAFKWVQKNILSYYPSTQIHAIAVGNEVFADPNNT 1342
L+G I+VTV LPNELLYS +K A WVQKN+++Y+PSTQI AIAVGNEVF DP+NT
Sbjct: 66 LSGCGIKVTVDLPNELLYSAAKNPYFARTWVQKNVVAYHPSTQIEAIAVGNEVFVDPHNT 125
Query: 1341 TNFLVPAMQNIHSALFKYNLHSAIKVSSPIALSALQSSYPSSAGSFKPVLIEPVIKPMLG 1162
T FL+PAM+NIH AL K+NLHS+IK+SSP+ALSALQSSYPSSAGSF+P LIEPV KPML
Sbjct: 126 TKFLIPAMRNIHQALVKFNLHSSIKISSPVALSALQSSYPSSAGSFRPELIEPVFKPMLD 185
Query: 1161 FLRQTGSYLMVNAYPFFAYEGNTDVISLDYALFRENPGVVDSGNGLRYFALFDAQVDAVF 982
FLRQTGSYLMVNAYPFFAYE N+DVISLDYAL RENPGVVDSGNGLRYF+LFDAQ+DAVF
Sbjct: 186 FLRQTGSYLMVNAYPFFAYESNSDVISLDYALLRENPGVVDSGNGLRYFSLFDAQIDAVF 245
Query: 981 AAMSALKFEDLKMVVTETGWPSKGDGIERGASEENAAAYNGNLVRRVINGGGTPLRPNEN 802
AA+SALK++D+K+VVTETGWPSKGD E G+ ENAAAYNGNLVRR++ GGGTPLRP +
Sbjct: 246 AALSALKYDDIKIVVTETGWPSKGDENEIGSGVENAAAYNGNLVRRILTGGGTPLRPQAD 305
Query: 801 LEVYLFALFNENKKPGPTSERNYGLFYPNEEKVYEIPFTPAQLKKWK-SKPVNRGGSEK- 628
L VYLFALFNEN+K GPTSERNYGLFYP+E+KVY+IPFT LK +K S + GS +
Sbjct: 306 LTVYLFALFNENEKDGPTSERNYGLFYPDEQKVYDIPFTVEGLKSYKDSNRSSDTGSHQV 365
Query: 627 -GPVNKG 610
PVN G
Sbjct: 366 AAPVNGG 372
Score = 164 bits (414), Expect(2) = 0.0
Identities = 74/103 (71%), Positives = 85/103 (82%)
Frame = -3
Query: 505 PVNKGASTRNMSGGTWCVANEKIRKEKLQAALDFACGEGGADCRQIQPGSTCYDPNTVEA 326
PVN G S ++ +G TWCVAN K+KLQA LDFACGEGGADCR IQPG+TCYDPNT+ A
Sbjct: 368 PVNGGVS-KSTTGKTWCVANPDAGKQKLQAGLDFACGEGGADCRPIQPGATCYDPNTLVA 426
Query: 325 HASFAFNSYYQRNGRAMGSCYFGGAAFVVTQLPKYGNCKFTTG 197
H+SFAFNSYYQ+ GR MG CYFGGAA+VVTQ PK+G C+F TG
Sbjct: 427 HSSFAFNSYYQKQGRGMGDCYFGGAAYVVTQEPKFGQCEFPTG 469
>ref|XP_002274828.1| PREDICTED: glucan endo-1,3-beta-glucosidase 3-like [Vitis vinifera]
Length = 471
Score = 544 bits (1401), Expect(2) = 0.0
Identities = 276/363 (76%), Positives = 306/363 (84%), Gaps = 2/363 (0%)
Frame = -1
Query: 1692 FLCFISIFILADAGQIGVNYGRVANNLPSAVKVVQLLKSQGIERVKLYDTDPSVLKALAG 1513
FL ISIF ADAG IGVNYGR+ANNLPSAVKVVQLLKSQGIERVK++DTDP+VLKAL
Sbjct: 9 FLLVISIFAFADAGSIGVNYGRIANNLPSAVKVVQLLKSQGIERVKVFDTDPAVLKALGE 68
Query: 1512 SNIEVTVALPNELLYSVSKTKSLAFKWVQKNILSYYPSTQIHAIAVGNEVFADPNNTTNF 1333
S I+VTV LPNELL S +K +S A WVQKN+ Y+P+T+I AIAVGNEVF DP+NTT
Sbjct: 69 SGIKVTVDLPNELLISAAKRQSFANTWVQKNVADYFPATKIEAIAVGNEVFVDPHNTTLS 128
Query: 1332 LVPAMQNIHSALFKYNLHSAIKVSSPIALSALQSSYPSSAGSFKPVLIEPVIKPMLGFLR 1153
LVPA++NIH AL KYNLHS IKVSSP+ALSALQSSYPSSAGSF+ LIEPV KPML FLR
Sbjct: 129 LVPALKNIHKALVKYNLHSHIKVSSPVALSALQSSYPSSAGSFRQELIEPVFKPMLEFLR 188
Query: 1152 QTGSYLMVNAYPFFAYEGNTDVISLDYALFRENPGVVDSGNGLRYFALFDAQVDAVFAAM 973
QTGSYLMVNAYPFFAYE N+DVISLDYALFRENPGVVD+GNGLRYF LFDAQ+DAVFAAM
Sbjct: 189 QTGSYLMVNAYPFFAYEANSDVISLDYALFRENPGVVDAGNGLRYFNLFDAQIDAVFAAM 248
Query: 972 SALKFEDLKMVVTETGWPSKGDGIERGASEENAAAYNGNLVRRVINGGGTPLRPNENLEV 793
SALK+ D+KMVVTETGWPSKGD E GAS NAAAYNGNLVRR++ GGGTPLRP +L V
Sbjct: 249 SALKYNDIKMVVTETGWPSKGDENEIGASVANAAAYNGNLVRRILTGGGTPLRPKADLVV 308
Query: 792 YLFALFNENKKPGPTSERNYGLFYPNEEKVYEIPFTPAQLKKWK--SKPVNRGGSEKGPV 619
+LFALFNENKKPGP+SERNYGLFYP+EEKVY IPFT LK ++ PV G PV
Sbjct: 309 FLFALFNENKKPGPSSERNYGLFYPSEEKVYNIPFTVEGLKNYRDIQSPVTGGVRVSTPV 368
Query: 618 NKG 610
+G
Sbjct: 369 TRG 371
Score = 155 bits (392), Expect(2) = 0.0
Identities = 71/104 (68%), Positives = 84/104 (80%), Gaps = 1/104 (0%)
Frame = -3
Query: 505 PVNKGASTR-NMSGGTWCVANEKIRKEKLQAALDFACGEGGADCRQIQPGSTCYDPNTVE 329
PV +G S + SG TWCVAN + KEKLQAALD+ACGEG ADC IQPG+TCYDPNT+E
Sbjct: 367 PVTRGGSVSPSSSGQTWCVANGETGKEKLQAALDYACGEGQADCHPIQPGATCYDPNTLE 426
Query: 328 AHASFAFNSYYQRNGRAMGSCYFGGAAFVVTQLPKYGNCKFTTG 197
AHASFAFNSYYQ+ GR +G+C F GAA+VVTQ P++G C+F TG
Sbjct: 427 AHASFAFNSYYQKKGRVIGTCDFQGAAYVVTQAPRFGKCEFPTG 470
>ref|XP_004140121.1| PREDICTED: glucan endo-1,3-beta-glucosidase 13-like isoform 2
[Cucumis sativus] gi|449481150|ref|XP_004156096.1|
PREDICTED: glucan endo-1,3-beta-glucosidase 13-like
isoform 2 [Cucumis sativus]
Length = 476
Score = 540 bits (1392), Expect(2) = 0.0
Identities = 269/365 (73%), Positives = 313/365 (85%), Gaps = 4/365 (1%)
Frame = -1
Query: 1692 FLCFISIFILADAGQIGVNYGRVANNLPSAVKVVQLLKSQGIERVKLYDTDPSVLKALAG 1513
FL F+S F A++G IGVNYGR+ N+LPSAVKVV+LLKS G++RVK+YDTDP+VLKAL+G
Sbjct: 13 FLLFLSPF--AESGSIGVNYGRIGNDLPSAVKVVKLLKSHGLQRVKVYDTDPAVLKALSG 70
Query: 1512 SNIEVTVALPNELLYSVSKTKSLAFKWVQKNILSYYPSTQIHAIAVGNEVFADPNNTTNF 1333
S I+VTV LPNELL++ +K + A+ WV+KN+ +YYPST+I AIAVGNEVF DP+NTT+F
Sbjct: 71 SGIKVTVDLPNELLFAAAKRLTFAYTWVEKNVAAYYPSTEIEAIAVGNEVFVDPHNTTSF 130
Query: 1332 LVPAMQNIHSALFKYNLHSAIKVSSPIALSALQSSYPSSAGSFKPVLIEPVIKPMLGFLR 1153
LVPAM+NIH AL KYNLHS IKVSSPIALSALQ+SYPSSAGSF+P L+E V +PML FLR
Sbjct: 131 LVPAMKNIHQALVKYNLHSNIKVSSPIALSALQNSYPSSAGSFRPELVETVFRPMLEFLR 190
Query: 1152 QTGSYLMVNAYPFFAYEGNTDVISLDYALFRENPGVVDSGNGLRYFALFDAQVDAVFAAM 973
QTGSYLMVNAYPFFAYE NTDVISLDYALFR+NPGVVD+G+G RYF LFDAQ+DAVFAAM
Sbjct: 191 QTGSYLMVNAYPFFAYESNTDVISLDYALFRDNPGVVDAGSGYRYFNLFDAQIDAVFAAM 250
Query: 972 SALKFEDLKMVVTETGWPSKGDGIERGASEENAAAYNGNLVRRVINGGGTPLRPNENLEV 793
SALK++D+KMVVTETGWPSKGD E GAS ENAAAYNGNLVRR+++GGGTPLRP +L V
Sbjct: 251 SALKYDDIKMVVTETGWPSKGDENEIGASVENAAAYNGNLVRRILSGGGTPLRPKADLTV 310
Query: 792 YLFALFNENKKPGPTSERNYGLFYPNEEKVYEIPFTPAQLKKWKS----KPVNRGGSEKG 625
YLFALFNENKK GPTSERNYGLFYPNEEKVY+IPFT LK ++ KPV+ G +
Sbjct: 311 YLFALFNENKKNGPTSERNYGLFYPNEEKVYDIPFTTEGLKDFEDKPSPKPVSGGNAPTA 370
Query: 624 PVNKG 610
P G
Sbjct: 371 PPASG 375
Score = 157 bits (396), Expect(2) = 0.0
Identities = 67/99 (67%), Positives = 85/99 (85%)
Frame = -3
Query: 493 GASTRNMSGGTWCVANEKIRKEKLQAALDFACGEGGADCRQIQPGSTCYDPNTVEAHASF 314
G +++ +G TWCVA+ + KEKLQ+ LD+ACGEGGADCR IQ G+TCY+PNT+EAHAS+
Sbjct: 377 GGVSKSQTGNTWCVASGEAGKEKLQSGLDYACGEGGADCRPIQVGATCYNPNTLEAHASY 436
Query: 313 AFNSYYQRNGRAMGSCYFGGAAFVVTQLPKYGNCKFTTG 197
AFNSYYQ+N R +G+CYFGGAA+VVTQ PKYG+C+F TG
Sbjct: 437 AFNSYYQKNSRKVGTCYFGGAAYVVTQPPKYGSCEFPTG 475
>ref|XP_002525335.1| Glucan endo-1,3-beta-glucosidase precursor, putative [Ricinus
communis] gi|223535394|gb|EEF37068.1| Glucan
endo-1,3-beta-glucosidase precursor, putative [Ricinus
communis]
Length = 495
Score = 538 bits (1386), Expect(2) = 0.0
Identities = 265/366 (72%), Positives = 310/366 (84%), Gaps = 2/366 (0%)
Frame = -1
Query: 1701 LTIFLCFISIFILADAGQIGVNYGRVANNLPSAVKVVQLLKSQGIERVKLYDTDPSVLKA 1522
++ L +S F ADAG IGVNYGR+A+NLPSA KVVQLLKSQG++RVK++D DP+VLKA
Sbjct: 6 ISSLLLLLSTFTFADAGSIGVNYGRIADNLPSATKVVQLLKSQGLQRVKVFDADPAVLKA 65
Query: 1521 LAGSNIEVTVALPNELLYSVSKTKSLAFKWVQKNILSYYPSTQIHAIAVGNEVFADPNNT 1342
L+G+ I+VTV LPNELLYS +K S AF WVQ+N+ YYPST+I AIAVGNEVF DP+NT
Sbjct: 66 LSGTRIKVTVDLPNELLYSAAKRPSFAFSWVQRNVAVYYPSTEIEAIAVGNEVFVDPHNT 125
Query: 1341 TNFLVPAMQNIHSALFKYNLHSAIKVSSPIALSALQSSYPSSAGSFKPVLIEPVIKPMLG 1162
T FL+PAM+NIH AL K NL SAIKVSSPIALSALQ+SYPSSAGSF+P LIEPV KP+
Sbjct: 126 TKFLLPAMRNIHQALQKLNLDSAIKVSSPIALSALQNSYPSSAGSFRPELIEPVFKPLFD 185
Query: 1161 FLRQTGSYLMVNAYPFFAYEGNTDVISLDYALFRENPGVVDSGNGLRYFALFDAQVDAVF 982
FLR+TGSYLMVNAYPFFAYE N+DVISLDYALFRENPGVVD+GNGLRYF+LFDAQ+DAVF
Sbjct: 186 FLRETGSYLMVNAYPFFAYESNSDVISLDYALFRENPGVVDAGNGLRYFSLFDAQIDAVF 245
Query: 981 AAMSALKFEDLKMVVTETGWPSKGDGIERGASEENAAAYNGNLVRRVINGGGTPLRPNEN 802
AAMSALK++D+ MV+TETGWPSKGD E GAS +NAA+YNGNLVRR++ GGGTPLRP+ +
Sbjct: 246 AAMSALKYDDISMVITETGWPSKGDENELGASVQNAASYNGNLVRRILTGGGTPLRPHAD 305
Query: 801 LEVYLFALFNENKKPGPTSERNYGLFYPNEEKVYEIPFTPAQLKKW--KSKPVNRGGSEK 628
L VYLFALFNE++K GPTSERNYGLFYPNE+KVY+IPFT LK + +S P G
Sbjct: 306 LTVYLFALFNEDRKNGPTSERNYGLFYPNEQKVYDIPFTVEGLKNYTDRSPPATAGQQVA 365
Query: 627 GPVNKG 610
PV+ G
Sbjct: 366 TPVSGG 371
Score = 149 bits (376), Expect(2) = 0.0
Identities = 68/93 (73%), Positives = 77/93 (82%)
Frame = -3
Query: 505 PVNKGASTRNMSGGTWCVANEKIRKEKLQAALDFACGEGGADCRQIQPGSTCYDPNTVEA 326
PV+ G S ++ +G TWCVAN + KEKLQ LD+ACGEGGADC IQPG TCYDPNTVEA
Sbjct: 367 PVSGGVS-KSTTGNTWCVANPDVGKEKLQHGLDYACGEGGADCHPIQPGETCYDPNTVEA 425
Query: 325 HASFAFNSYYQRNGRAMGSCYFGGAAFVVTQLP 227
HASFAFNSYYQ+ GR MG+CYFGGAA+VVTQ P
Sbjct: 426 HASFAFNSYYQKKGREMGTCYFGGAAYVVTQPP 458