BLASTX nr result
ID: Coptis24_contig00005190
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00005190
(1933 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 821 0.0
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 803 0.0
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 702 0.0
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 629 e-177
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 627 e-177
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 821 bits (2121), Expect = 0.0
Identities = 413/464 (89%), Positives = 417/464 (89%)
Frame = +2
Query: 251 RQKNLPPGPRPLPIVGNLLQLGDKPHAEFAKLAQKYGQLFSLKLGSQTXXXXXXXXXXXX 430
RQKNLPPGPRPLPIVGNLLQLGDKP AEFAKLAQKYGQLFSLKLGSQT
Sbjct: 25 RQKNLPPGPRPLPIVGNLLQLGDKPRAEFAKLAQKYGQLFSLKLGSQTVVVASSPAAAAE 84
Query: 431 XLKTHDKILSGRYVFQSFRVKEHIENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 610
LKTHDKILSGRYVFQSFRVKEH+ENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI
Sbjct: 85 VLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 144
Query: 611 REGKAREMVKFLRSXXXXXXXXXXXXFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL 790
REGKAREMVKFLRS FGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL
Sbjct: 145 REGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL 204
Query: 791 WKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIYDVWGINLKERRGMKGSESNND 970
WKLLEMGNSTNPADYFPIMGKYDLFGQRRAVA+VLQQIYDVWGINLKERRGMKGSESNND
Sbjct: 205 WKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNND 264
Query: 971 FVDVLLNAGLEDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 1150
FVDVLLNAGLEDQKINALLMELFG GTETSASTIEWAITELTKKPLVVSKIRLELVNVVG
Sbjct: 265 FVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 324
Query: 1151 DNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXXCTVMNYTIPKECQIMVNAWA 1330
DNTVKESDLPHLPYLQAFVKE CTVMNYTIPKECQIMVNAWA
Sbjct: 325 DNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWA 384
Query: 1331 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFINLLVATL 1510
IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFI+L+VATL
Sbjct: 385 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATL 444
Query: 1511 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARPSNI 1642
VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARPS I
Sbjct: 445 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARPSYI 488
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 803 bits (2075), Expect = 0.0
Identities = 404/464 (87%), Positives = 410/464 (88%)
Frame = +2
Query: 251 RQKNLPPGPRPLPIVGNLLQLGDKPHAEFAKLAQKYGQLFSLKLGSQTXXXXXXXXXXXX 430
R KNLPPGPRP PIVGNLLQLGDKPHAEFAKLAQKYG+LFSLKLGSQT
Sbjct: 25 RPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAE 84
Query: 431 XLKTHDKILSGRYVFQSFRVKEHIENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 610
LKTHDKILSGRYVFQSFRVKEH+ENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI
Sbjct: 85 ILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 144
Query: 611 REGKAREMVKFLRSXXXXXXXXXXXXFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL 790
RE KAREMVKFLR FGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL
Sbjct: 145 REAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL 204
Query: 791 WKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIYDVWGINLKERRGMKGSESNND 970
WKLL+MGNSTNPADYFPIMGK DLFGQRRAVAEVLQQIYDVWG+ LKERRG KGSES ND
Sbjct: 205 WKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKND 264
Query: 971 FVDVLLNAGLEDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 1150
FVDVLLNAGL+DQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG
Sbjct: 265 FVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 324
Query: 1151 DNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXXCTVMNYTIPKECQIMVNAWA 1330
DNTVKESDLPHLPYLQAFVKE CTVMNYTIPKECQIMVNAWA
Sbjct: 325 DNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWA 384
Query: 1331 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFINLLVATL 1510
IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQF NL+VATL
Sbjct: 385 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATL 444
Query: 1511 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARPSNI 1642
VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR SNI
Sbjct: 445 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARASNI 488
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 702 bits (1811), Expect = 0.0
Identities = 348/462 (75%), Positives = 382/462 (82%)
Frame = +2
Query: 251 RQKNLPPGPRPLPIVGNLLQLGDKPHAEFAKLAQKYGQLFSLKLGSQTXXXXXXXXXXXX 430
R K+LPPGPRP PIVGNLLQLG+KPHAEFAKLA+KYG+LF+LKLGSQT
Sbjct: 26 RNKDLPPGPRPSPIVGNLLQLGEKPHAEFAKLAEKYGELFTLKLGSQTVVVASSPAAAAE 85
Query: 431 XLKTHDKILSGRYVFQSFRVKEHIENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEI 610
LKT DKILSGRYVFQSFRV EH+ NSIVWSECN+NWKLLRKVCRTELF+PKMIESQ+ I
Sbjct: 86 ILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIESQAYI 145
Query: 611 REGKAREMVKFLRSXXXXXXXXXXXXFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHL 790
RE KA +MV+FLR F TLVNIFGNLIFSKDVFDLEDPTGGS ELKEHL
Sbjct: 146 REAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAELKEHL 205
Query: 791 WKLLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIYDVWGINLKERRGMKGSESNND 970
WKLL+MGNSTNPADYFPIMGK+DLFGQRR VA+VL+QIYDVWG+ LKERR + G ND
Sbjct: 206 WKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITG-HREND 264
Query: 971 FVDVLLNAGLEDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 1150
F +VLLNAGL+DQKINALLMELFGAGTET+ASTIEWAITELT V+SK+R EL+NVVG
Sbjct: 265 FANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVG 324
Query: 1151 DNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXXCTVMNYTIPKECQIMVNAWA 1330
TVKES++PHLPYLQA VKE C VMNYTIPKECQIMVNAWA
Sbjct: 325 HKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWA 384
Query: 1331 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFINLLVATL 1510
IGRDPKTWDDPL FKPERF++S VDYKGNDFELIPFGGGRRICPGLPLASQF++L+VATL
Sbjct: 385 IGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIPFGGGRRICPGLPLASQFLSLIVATL 444
Query: 1511 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARPS 1636
VQNF+WS PQGM+ +E+ MDEKFGL LQKDPPLLIVLKAR S
Sbjct: 445 VQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLIVLKARTS 486
>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 629 bits (1621), Expect = e-177
Identities = 302/460 (65%), Positives = 363/460 (78%), Gaps = 2/460 (0%)
Frame = +2
Query: 257 KNLPPGPRPLPIVGNLLQLGDKPHAEFAKLAQKYGQLFSLKLGSQTXXXXXXXXXXXXXL 436
K LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG LFSLKLGS+T L
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEIL 80
Query: 437 KTHDKILSGRYVFQSFRVKEHIENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIRE 616
KTHD++LSGRYVFQSFRVKEH+ENSIVWSECN+ WK LRKVCRTELFT KMIESQ+E+RE
Sbjct: 81 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEVRE 140
Query: 617 GKAREMVKFLRSXXXXXXXXXXXXFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWK 796
KA EMV++L+ FGTLVNIFGNLIFS+++F L D + GSVE+KEHLW+
Sbjct: 141 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 200
Query: 797 LLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIYDVWGINLKERRGMK--GSESNND 970
+LE+GNSTNPADYFP +GK+DLFGQ + VA+ LQ IY VWG LKER+ K + ND
Sbjct: 201 MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKND 260
Query: 971 FVDVLLNAGLEDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 1150
FV++LL++GL+DQ+INALLME+FGAGTETSASTIEWA++ELTK P V + +RLEL++VVG
Sbjct: 261 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVG 320
Query: 1151 DNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXXCTVMNYTIPKECQIMVNAWA 1330
VKESD+P++PYLQAFVKE C V+NYTIPKECQIMVNAW
Sbjct: 321 KRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 380
Query: 1331 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFINLLVATL 1510
IGRDPK W DPL F PERFL+S +D+KGNDFELIPFG GRRICPG+PLA+QFI+L+V++L
Sbjct: 381 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSL 440
Query: 1511 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 1630
VQNF+W P+GM S+L M+EKFGLTLQK+PPL IV K R
Sbjct: 441 VQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 627 bits (1616), Expect = e-177
Identities = 301/460 (65%), Positives = 363/460 (78%), Gaps = 2/460 (0%)
Frame = +2
Query: 257 KNLPPGPRPLPIVGNLLQLGDKPHAEFAKLAQKYGQLFSLKLGSQTXXXXXXXXXXXXXL 436
K LPPGP+P PIVGNLLQLG+KPH++FA+LA+ YG LFSLKLGS+T L
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEIL 80
Query: 437 KTHDKILSGRYVFQSFRVKEHIENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIRE 616
KTHD++LSGRYVFQSFRVKEH+ENSIVWSECN+ WK LRKVCRT+LFT KMIESQ+E+RE
Sbjct: 81 KTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEVRE 140
Query: 617 GKAREMVKFLRSXXXXXXXXXXXXFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWK 796
KA EMV++L+ FGTLVNIFGNLIFS+++F L D + GSVE+KEHLW+
Sbjct: 141 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKEHLWR 200
Query: 797 LLEMGNSTNPADYFPIMGKYDLFGQRRAVAEVLQQIYDVWGINLKERRGMK--GSESNND 970
+LE+GNSTNPADYFP +GK+DLFGQ + VA+ LQ IY VWG LKE + K + ND
Sbjct: 201 MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKND 260
Query: 971 FVDVLLNAGLEDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVG 1150
FV++LL++GL+DQ+INALLME+FGAGTETSASTIEWA++ELTK P V + +RLEL++VVG
Sbjct: 261 FVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVG 320
Query: 1151 DNTVKESDLPHLPYLQAFVKEXXXXXXXXXXXXXXXXXXXCTVMNYTIPKECQIMVNAWA 1330
VKESD+P++PYLQAFVKE C V+NYTIPKECQIMVNAW
Sbjct: 321 KRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWG 380
Query: 1331 IGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFINLLVATL 1510
IGRDPK W DPL F PERFL+S +D+KGNDFELIPFG GRRICPG+PLA+QFI+L+V++L
Sbjct: 381 IGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSL 440
Query: 1511 VQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 1630
VQNF+W LP+GM S+L M+EKFGLTLQK+PPL IV K R
Sbjct: 441 VQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480