BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00003538
         (2136 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;...   650   0.0  
dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]      468   e-129
sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltran...   448   e-123
gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransf...   432   e-118
gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]       430   e-118

>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
            gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
            [Thalictrum flavum]
          Length = 356

 Score =  650 bits (1678), Expect = 0.0
 Identities = 311/355 (87%), Positives = 336/355 (94%)
 Frame = +2

Query: 761  METKHAKKEAIADFIKRMELGQVPDEEIRKKMRTLVEKRLKWGYKPTHEQQLAHLISFIQ 940
            METK  KKEA+A+ IKR+E G+V DEEIR  M+  V+KRLKWGYKPTHEQQLA L++F Q
Sbjct: 1    METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60

Query: 941  SLRSMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYC 1120
            SL+ MEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESE+ M+DLYC
Sbjct: 61   SLKGMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYC 120

Query: 1121 ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVE 1300
            ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMD+CKKLDLSNVE
Sbjct: 121  ERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVE 180

Query: 1301 IILADVTKFETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYK 1480
            IIL DVTKFETE TYDRIFAVALIEHMKNYELFLK+VS W+AQ GLLFVEHHCHKVFAY+
Sbjct: 181  IILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQ 240

Query: 1481 YEPLGDDDWYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLD 1660
            YEPL +DDWYTEYIFP+GTLVMSSSSILLYFQEDVS++NHWTLSGKHPSLGFK+WLKRLD
Sbjct: 241  YEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLD 300

Query: 1661 DNMDAVKEIFESFYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1825
            DN+D VKEIFESFYGSKEKAMK+ITYWRVFCIAHS+MY++NNGEEWMLSQVLFKK
Sbjct: 301  DNIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKK 355


>dbj|BAB71802.1| coclaurine N-methyltransferase [Coptis japonica]
          Length = 358

 Score =  468 bits (1203), Expect = e-129
 Identities = 214/357 (59%), Positives = 285/357 (79%)
 Frame = +2

Query: 755  MSMETKHAKKEAIADFIKRMELGQVPDEEIRKKMRTLVEKRLKWGYKPTHEQQLAHLISF 934
            M++E K  KK AI + +K++ELG VP ++I++ +R  + +RL+WGYKPT+E+Q+A + + 
Sbjct: 1    MAVEAKQTKKAAIVELLKQLELGLVPYDDIKQLIRRELARRLQWGYKPTYEEQIAEIQNL 60

Query: 935  IQSLRSMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDL 1114
              SLR M++A EV+TLD++LYEIP+ FL IM G  LK S  YFK++STTLDE+EI MLDL
Sbjct: 61   THSLRQMKIATEVETLDSQLYEIPIEFLKIMNGSNLKGSCCYFKEDSTTLDEAEIAMLDL 120

Query: 1115 YCERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSN 1294
            YCERAQI+DGQS+LDLGCG G+LTLHVAQKY+ C+VT +TNSVSQKE+I +E ++ +L N
Sbjct: 121  YCERAQIQDGQSVLDLGCGQGALTLHVAQKYKNCRVTAVTNSVSQKEYIEEESRRRNLLN 180

Query: 1295 VEIILADVTKFETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFA 1474
            VE+ LAD+T  E   TYDRI  + L EHMKNYEL L+++S+W+++DGLLF+EH CHK FA
Sbjct: 181  VEVKLADITTHEMAETYDRILVIELFEHMKNYELLLRKISEWISKDGLLFLEHICHKTFA 240

Query: 1475 YKYEPLGDDDWYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKR 1654
            Y YEPL DDDW+TEY+FP GT+++ S+S  LYFQ+DVS++NHWTLSGKH S   +EWLKR
Sbjct: 241  YHYEPLDDDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKR 300

Query: 1655 LDDNMDAVKEIFESFYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1825
            LD N+D +K +FE+  G++E+A+K I YWR FC++  EM+  NNGEEWM S VLFKK
Sbjct: 301  LDANLDVIKPMFETLMGNEEEAVKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 357


>sp|Q5C9L6.1|CNMT_THLFG RecName: Full=(S)-coclaurine N-methyltransferase; Short=TfCNMT
            gi|51980204|gb|AAU20766.1| (S)-coclaurine
            N-methyltransferase [Thalictrum flavum subsp. glaucum]
          Length = 361

 Score =  448 bits (1153), Expect = e-123
 Identities = 211/360 (58%), Positives = 281/360 (78%), Gaps = 3/360 (0%)
 Frame = +2

Query: 755  MSMETKHA--KKEAIADFIKRMELGQVPDEEIRKKMRTLVEKRLKWGYKPTHEQQLAHLI 928
            M++E K    KK  I + +K++ELG VPD+EI+K +R  + +RL+WG K T+E+Q+A L+
Sbjct: 1    MAVEGKQVAPKKAIIVELLKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLV 60

Query: 929  SFIQSLRSMEMAEEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINML 1108
            +   SLR M++A EV+TLD ++YE+P+ FL IM G  LK S  YFK++STTLDE+EI ML
Sbjct: 61   NLTHSLRQMKIATEVETLDDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAML 120

Query: 1109 DLYCERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDL 1288
            +LYCERAQIKDG S+LDLGCG G+LTL+VAQKY+  +VT +TNSVSQKEFI +E +K +L
Sbjct: 121  ELYCERAQIKDGHSVLDLGCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNL 180

Query: 1289 SNVEIILADVTKFETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKV 1468
            SNVE++LAD+T  +  +TYDRI  V L EHMKNYEL L+++ +WMA+DGLLFVEH CHK 
Sbjct: 181  SNVEVLLADITTHKMPDTYDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKT 240

Query: 1469 FAYKYEPLGDDDWYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWL 1648
            FAY YEP+ +DDW+TEY+FP GT+++ S+S  LYFQ+DVS++NHWTLSGKH S   +EWL
Sbjct: 241  FAYHYEPIDEDDWFTEYVFPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWL 300

Query: 1649 KRLDDNMDAVKEIFESFYGS-KEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1825
            KRLD N++ +K +F +  G  +++AMK I YWR FC++  EM+  NNGEEWM S VLFKK
Sbjct: 301  KRLDANVELIKPMFVTITGQCRQEAMKLINYWRGFCLSGMEMFGYNNGEEWMASHVLFKK 360


>gb|AAP45316.1| S-adenosyl-L-methionine:coclaurine N-methyltransferase [Papaver
            somniferum]
          Length = 351

 Score =  432 bits (1112), Expect = e-118
 Identities = 197/347 (56%), Positives = 268/347 (77%)
 Frame = +2

Query: 785  EAIADFIKRMELGQVPDEEIRKKMRTLVEKRLKWGYKPTHEQQLAHLISFIQSLRSMEMA 964
            +A  + ++ MELG +PD+EIR+ +R  +EKRL+WGYK THE+QL+ L+  + SL+ M+MA
Sbjct: 4    KAKEELLRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMA 63

Query: 965  EEVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIKDG 1144
             E++ LD +LYE P+ FL I  G  +K S GY+ DESTTLDE+EI MLDLY ERAQIKDG
Sbjct: 64   TEMENLDLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDG 123

Query: 1145 QSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADVTK 1324
            QS+LDLGCG G++ L  A K++ C+ TG+T+SV QK++I  +CK+L L+NV+++LAD+T 
Sbjct: 124  QSVLDLGCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITT 183

Query: 1325 FETENTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLGDDD 1504
            +ETE  +DRIFAV LIEHMKNY+L LK++S+WM  DGLLFVEH CHK  AY YEP+  +D
Sbjct: 184  YETEERFDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAED 243

Query: 1505 WYTEYIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAVKE 1684
            WYT YIFP GTL +SS+S+LLYFQ+DVS++N WTLSGKH S   +EWLK +D N+   KE
Sbjct: 244  WYTNYIFPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKE 303

Query: 1685 IFESFYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1825
            I  S   ++++A+K + +WR+FC+  +E++   NGEEWML+ +LFKK
Sbjct: 304  IMRSITKTEKEAIKLLNFWRIFCMCGAELFGYKNGEEWMLTHLLFKK 350


>gb|ACO90253.1| putative N-methyltransferase [Thalictrum flavum]
          Length = 351

 Score =  430 bits (1106), Expect = e-118
 Identities = 198/343 (57%), Positives = 261/343 (76%)
 Frame = +2

Query: 797  DFIKRMELGQVPDEEIRKKMRTLVEKRLKWGYKPTHEQQLAHLISFIQSLRSMEMAEEVD 976
            +  K++ELG +P++E++K MR  + +RL+WGYK T+E+Q+A ++    SLR M +A EVD
Sbjct: 8    ELFKKLELGLIPNDEVKKLMRIQLARRLQWGYKSTYEEQIAQVLDLTHSLRHMNIAMEVD 67

Query: 977  TLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEINMLDLYCERAQIKDGQSIL 1156
            TLD+ +YE+PL FL IM G  LK S  YFKD+STTLDE+EI M+DL+CERAQIKDG S+L
Sbjct: 68   TLDSNMYEVPLDFLKIMNGSALKMSSCYFKDDSTTLDEAEIAMMDLHCERAQIKDGHSVL 127

Query: 1157 DLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDECKKLDLSNVEIILADVTKFETE 1336
            DLGCGHGSLT +VAQKY+   VT +TNSVSQKE+I +E ++ +LSNVE++LAD+   E  
Sbjct: 128  DLGCGHGSLTFYVAQKYKKSHVTAVTNSVSQKEYIEEEARRRNLSNVEVLLADIATHEMA 187

Query: 1337 NTYDRIFAVALIEHMKNYELFLKEVSKWMAQDGLLFVEHHCHKVFAYKYEPLGDDDWYTE 1516
            +TYDRI  V L EHMKNY L LK++S+WMA+DGLLFV+H CHK FA+  EP+ +DDW +E
Sbjct: 188  DTYDRILVVGLFEHMKNYGLLLKKISEWMAKDGLLFVQHVCHKTFAFHCEPIDEDDWLSE 247

Query: 1517 YIFPTGTLVMSSSSILLYFQEDVSILNHWTLSGKHPSLGFKEWLKRLDDNMDAVKEIFES 1696
            Y FP GT+++ S+S  LYFQ+D S++NHWTLSGKH S   +EWLKRLD N+  +  +F +
Sbjct: 248  YAFPAGTMIIPSASFFLYFQDDASVVNHWTLSGKHFSRTTEEWLKRLDANVKVIIPMFAT 307

Query: 1697 FYGSKEKAMKWITYWRVFCIAHSEMYASNNGEEWMLSQVLFKK 1825
              GS+E+A+K + YWR  C    E+Y  NNGEEWM S VLFKK
Sbjct: 308  ITGSEEEAVKLLNYWRGLCFFGVELYGYNNGEEWMESHVLFKK 350