BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00003514
         (2036 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510136.1| cytochrome P450, putative [Ricinus communis]...   479   e-133
ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis ...   476   e-132
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   465   e-128
ref|XP_002301158.1| predicted protein [Populus trichocarpa] gi|2...   462   e-127
ref|XP_002327187.1| predicted protein [Populus trichocarpa] gi|2...   455   e-125

>ref|XP_002510136.1| cytochrome P450, putative [Ricinus communis]
            gi|223550837|gb|EEF52323.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 505

 Score =  479 bits (1234), Expect = e-133
 Identities = 241/485 (49%), Positives = 326/485 (67%), Gaps = 2/485 (0%)
 Frame = +1

Query: 355  PTNKNLPPSPFALPIIGHLHLFKQPLLRTVQTLSQKYGPIVFLEIGTRPVLLISSVSLVE 534
            P  +NLPP PF+LP+IGHLH+ K+PL   ++TL  KYGPI+ L+ G R VL++SS S VE
Sbjct: 23   PQKQNLPPGPFSLPLIGHLHMLKKPLPLALETLLSKYGPILSLKFGYRSVLVVSSPSAVE 82

Query: 535  ECFTKNDVIFANRPQTVVADHVSYKYSTVVVSPYGPHWRNLRRITTIEIFSSKSLQTXXX 714
            ECFTKND+IFANRP+++  DH +Y Y+T V +PYG  WR+LRR+T +E+FSSKSLQ    
Sbjct: 83   ECFTKNDIIFANRPKSMAGDHFTYNYTTYVWAPYGELWRSLRRLTVVELFSSKSLQNNSA 142

Query: 715  XXXXXXXXLIKNLFQSYNGGLQHFDLKLMFSKLMFNVMTRMVCGKRCFEESMDFDE-QKR 891
                    L+  LF+   GG Q  DLK + S LM NVM ++  GK C +E ++  E +K+
Sbjct: 143  IRKQEVHRLVSRLFKVSAGGKQKVDLKFLLSLLMCNVMMKIAVGKLCVKEEIEGTEVEKQ 202

Query: 892  VHDHFKELFSPMLALGLQDFLPILRKFGLSRREKSILKLHKKRDSFLQDMINEFRKRRIS 1071
            ++  FK+ F P L L + DF+P LR  G    EK+++K+  KRD FLQD+++  R  R  
Sbjct: 203  LYQGFKDKFFPSLTLNVCDFIPFLRMIGFKGIEKNMIKMQNKRDEFLQDLLDGIRLNRKH 262

Query: 1072 STTATADESKTIIEALLSIQEADPEHYPDEFIKGIVLTMFVAGVDTMSLTLEWAFSLLVN 1251
            S T+  +E +++ E LLS+QE +P  Y D+ IK I++ MF+AGV+T ++ LEWA SLL+ 
Sbjct: 263  SKTSDDEEKRSVAETLLSLQELEPAIYTDDVIKSIMVMMFIAGVETSAVALEWAMSLLLI 322

Query: 1252 NPDKLHKARSEIDSHV-QGHVLDDSDLTNLPYLHCIIHETXXXXXXXXXXXXXXSSEDCT 1428
            +P+ L K R+EID+ V  G +L+D DL  LPYL C+++ET              SSE CT
Sbjct: 323  HPEILQKLRAEIDNCVGHGRLLNDLDLVKLPYLRCVVNETLRLYPPGPLMLPHLSSEICT 382

Query: 1429 VGGYNIPRGTILFTNIWGIHRDPKVWEEPTTFIPERFEDVHGEREMGIKFSPFGVGRRSC 1608
            VGG+ I +GT+L  N+W +HRDP VWEEPT F PERFE   GE E   KF PFG+GRR+C
Sbjct: 383  VGGFEIQKGTLLMVNLWAMHRDPNVWEEPTEFKPERFEGDLGE-EHAFKFMPFGMGRRAC 441

Query: 1609 PGAGMSMRMLPLALGTLIQCFEWERVGREQVEMDEHSTGVTMPKAMPLVAMCKPRSNMVN 1788
            PGAGM  RM+ LALG LIQ FEWE+ G E+V+M+    G+++ KA PLV +C PR  MV 
Sbjct: 442  PGAGMGTRMVSLALGALIQSFEWEKDGLEKVDMNPR-FGMSLSKAKPLVVLCCPRPEMVE 500

Query: 1789 ALSQI 1803
             LSQ+
Sbjct: 501  VLSQL 505


>ref|XP_002285066.1| PREDICTED: cytochrome P450 81F1-like [Vitis vinifera]
          Length = 507

 Score =  476 bits (1226), Expect = e-132
 Identities = 232/480 (48%), Positives = 322/480 (67%), Gaps = 2/480 (0%)
 Frame = +1

Query: 370  LPPSPFALPIIGHLHLFKQPLLRTVQTLSQKYGPIVFLEIGTRPVLLISSVSLVEECFTK 549
            LPPSPFALP+IGHLHL K PL + +Q LS +YGPI+FL  G RP +++SS S V+ECFTK
Sbjct: 32   LPPSPFALPVIGHLHLLKPPLYQGLQALSSQYGPILFLRFGCRPFVVVSSPSAVQECFTK 91

Query: 550  NDVIFANRPQTVVADHVSYKYSTVVVSPYGPHWRNLRRITTIEIFSSKSLQTXXXXXXXX 729
            NDV+ ANRP++++ DHV+Y Y+    + YG  WR LRR+T +EI SS  L          
Sbjct: 92   NDVVLANRPRSMIGDHVTYNYTAFAWASYGHLWRVLRRLTVVEILSSNKLLLLSTVREEE 151

Query: 730  XXXLIKNLFQSYNGGLQHFDLKLMFSKLMFNVMTRMVCGKRCFEESMD-FDEQKRVHDHF 906
               L++ LF+  N G Q  D++L  S   FN + + + GKRC EE  +  +  ++  +  
Sbjct: 152  VRYLLRQLFKVSNDGAQKVDMRLYLSLFSFNFIMKTITGKRCIEEEAEGIETNRQFLERL 211

Query: 907  KELFSPMLALGLQDFLPILRKFGLSRREKSILKLHKKRDSFLQDMINEFRKRRISSTTAT 1086
            K +F P     L DF PILR  G    EKS+++  K+RD +LQ M++EFR+   +++   
Sbjct: 212  KRIFVPTTTTNLCDFFPILRWVGYKGLEKSVIQFGKERDGYLQGMLDEFRR---NNSAVE 268

Query: 1087 ADESKTIIEALLSIQEADPEHYPDEFIKGIVLTMFVAGVDTMSLTLEWAFSLLVNNPDKL 1266
              + +T+IE LL +Q+++P+ Y D+ IKG++L +  AG DT S+TLEWA SLL+N+P+ L
Sbjct: 269  WQKKRTLIETLLFLQQSEPDFYTDDVIKGLMLVVISAGTDTSSVTLEWAMSLLLNHPEAL 328

Query: 1267 HKARSEIDSHVQ-GHVLDDSDLTNLPYLHCIIHETXXXXXXXXXXXXXXSSEDCTVGGYN 1443
             KAR+EIDSHV+ GH+LDDSDL  LPYL  +++ET              SSEDC+VGG++
Sbjct: 329  EKARAEIDSHVKPGHLLDDSDLAKLPYLRSVVNETLRLYPTAPLLLPHLSSEDCSVGGFD 388

Query: 1444 IPRGTILFTNIWGIHRDPKVWEEPTTFIPERFEDVHGEREMGIKFSPFGVGRRSCPGAGM 1623
            IPRGT +  N+W +HRDP+VWEE T F PERFE +  E +   KF+PFG+GRR+CPGA +
Sbjct: 389  IPRGTTVMVNVWALHRDPRVWEEATKFKPERFEGMENEEKEAFKFAPFGIGRRACPGAAL 448

Query: 1624 SMRMLPLALGTLIQCFEWERVGREQVEMDEHSTGVTMPKAMPLVAMCKPRSNMVNALSQI 1803
            +M+++ LALG LIQCFEWERV  E+V+M   S G+TMPKA PL  + +PR  M + LSQ+
Sbjct: 449  AMKIVSLALGGLIQCFEWERVEAEKVDMSPGS-GITMPKAKPLEIIFRPRPTMTSLLSQL 507


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  465 bits (1197), Expect = e-128
 Identities = 229/486 (47%), Positives = 319/486 (65%), Gaps = 3/486 (0%)
 Frame = +1

Query: 355  PTNKNLPPSPFALPIIGHLHLFKQPLLRTVQTLSQKYGPIVFLEIGTRPVLLISSVSLVE 534
            P +KNLPPSP +LPIIGHLHLFK+PL RT+  +S +YGP+++ + G+RPVL++SS S  E
Sbjct: 25   PKHKNLPPSPPSLPIIGHLHLFKKPLYRTLTNISNQYGPVLYFQFGSRPVLVVSSPSAAE 84

Query: 535  ECFTKNDVIFANRPQTVVADHVSYKYSTVVVSPYGPHWRNLRRITTIEIFSSKSLQTXXX 714
            EC TKND+ FANRP+ ++  H    Y+T+  + YG +WRNLRRI+ +E+FS   +Q    
Sbjct: 85   ECLTKNDIGFANRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSE 144

Query: 715  XXXXXXXXLIKNLFQSYNGGLQHFDLKLMFSKLMFNVMTRMVCGKRCFEESM-DFDEQKR 891
                    +++ L   Y       ++K +F +L  N+M RM+ GKR + E + D ++ ++
Sbjct: 145  IRTDEVKSMVRRLVAGYE--YHKVEMKSVFFELTLNIMMRMIAGKRYYGEHVADLEQARK 202

Query: 892  VHDHFKELFSPMLALGLQDFLPILRKFGLSRREKSILKLHKKRDSFLQDMINEFRKRRIS 1071
              +  +E F    A  + DFLP+LR  G    EK +++L  KRD FLQ++I E RK R++
Sbjct: 203  FKEIVEETFELSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVN 262

Query: 1072 STTATADESK-TIIEALLSIQEADPEHYPDEFIKGIVLTMFVAGVDTMSLTLEWAFSLLV 1248
            S +  ++E K T+I+ LLS+QE +PE+Y DE I+G++  +F AG DT + T+EWA SLL+
Sbjct: 263  SKSFPSEEKKKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLL 322

Query: 1249 NNPDKLHKARSEIDSHVQ-GHVLDDSDLTNLPYLHCIIHETXXXXXXXXXXXXXXSSEDC 1425
            NNPD + KA+SEID  ++ G  +D+SDL  LPYLHCII+ET              SSEDC
Sbjct: 323  NNPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDC 382

Query: 1426 TVGGYNIPRGTILFTNIWGIHRDPKVWEEPTTFIPERFEDVHGEREMGIKFSPFGVGRRS 1605
             +GGYNIP GT+L  N+W I  DP +W+EP  F PERFE   G    G K  PFG GRR 
Sbjct: 383  EIGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRG 442

Query: 1606 CPGAGMSMRMLPLALGTLIQCFEWERVGREQVEMDEHSTGVTMPKAMPLVAMCKPRSNMV 1785
            CPG G++ R++ L L +L+QCF+W RVG E VEM E  TG+T+PK  PL A C+PRS M+
Sbjct: 443  CPGEGLAXRVVGLTLXSLLQCFDWHRVGEEMVEMSE-GTGLTLPKLHPLEAHCRPRSTML 501

Query: 1786 NALSQI 1803
            N LSQ+
Sbjct: 502  NFLSQV 507


>ref|XP_002301158.1| predicted protein [Populus trichocarpa] gi|222842884|gb|EEE80431.1|
            predicted protein [Populus trichocarpa]
          Length = 507

 Score =  462 bits (1189), Expect = e-127
 Identities = 237/484 (48%), Positives = 320/484 (66%), Gaps = 7/484 (1%)
 Frame = +1

Query: 364  KNLPPSPFALPIIGHLHLFKQPLLRTVQTLSQKYGPIVFLEIGTRPVLLISSVSLVEECF 543
            +NLPPSPFALPIIGHLHLFK P  +++QTLS +YGPI+FL+ G R  L++SS S VEECF
Sbjct: 26   QNLPPSPFALPIIGHLHLFKHP--QSLQTLSSQYGPILFLKFGCRSTLVVSSPSAVEECF 83

Query: 544  TKNDVIFANRPQTVVADHVSYKYSTVVVSPYGPHWRNLRRITTIEIFSSKSLQTXXXXXX 723
            TKND+IFANRPQ++  DH++Y Y+  V +PYG  WR+LRRI+ IEIF+SKSLQ       
Sbjct: 84   TKNDIIFANRPQSMAGDHLTYNYTGFVWAPYGHLWRSLRRISVIEIFASKSLQKSSIIRE 143

Query: 724  XXXXXLIKNLFQSYNGGLQHFDLKLMFSKLMFNVMTRMVCGKRCFEESMDFDE-QKRVHD 900
                 L++ L ++ NG     DLK +FS L  NVM R+  GK C +E +   + +K++  
Sbjct: 144  EEVCSLLRRLLKAKNGVTAKVDLKFLFSLLTCNVMMRLAAGKPCIDEEVAGTKVEKQLFQ 203

Query: 901  HFKELFSPMLALGLQDFLPILRKFGLSRREKSILKLHKKRDSFLQDMINEFRKRRISSTT 1080
             FKE FSP L + + DF+PILR  G    EKS  KL   RD +LQ +I+E R RR SS++
Sbjct: 204  EFKERFSPGLGMNICDFIPILRLIGYKGLEKSTKKLQSTRDKYLQHLIDEIRMRRTSSSS 263

Query: 1081 ATADESK-----TIIEALLSIQEADPEHYPDEFIKGIVLTMFVAGVDTMSLTLEWAFSLL 1245
             TA++ K     ++IE  LS+Q+ +PE   D  IK ++  MFVAG +T ++TLEWA +LL
Sbjct: 264  KTAEQWKREGKSSVIETFLSLQDLEPEFLTDTVIKSVLSMMFVAGTETSAVTLEWAMALL 323

Query: 1246 VNNPDKLHKARSEIDSHV-QGHVLDDSDLTNLPYLHCIIHETXXXXXXXXXXXXXXSSED 1422
            +N+P  + K ++EID HV  G +L++S++  LPYL C+I ET              SS  
Sbjct: 324  LNHPKAMQKLKAEIDEHVGHGRLLNESNIVKLPYLRCVIKETLRLYPPAPLLLPHFSSGA 383

Query: 1423 CTVGGYNIPRGTILFTNIWGIHRDPKVWEEPTTFIPERFEDVHGEREMGIKFSPFGVGRR 1602
            CTVGG++IP+GT L  N W +HRDPK+WEE   F PERFE   GE+E G K+ PFG GRR
Sbjct: 384  CTVGGFDIPQGTTLVVNAWAMHRDPKLWEESNEFKPERFEAGLGEQE-GFKYIPFGTGRR 442

Query: 1603 SCPGAGMSMRMLPLALGTLIQCFEWERVGREQVEMDEHSTGVTMPKAMPLVAMCKPRSNM 1782
             CPGA M ++M+ +ALG L+QCFEW++V    VE   HS G+++ K  PL A+C PR ++
Sbjct: 443  VCPGASMGLQMVSIALGALVQCFEWDKVA--PVEDMSHSPGISLSKVKPLEALCCPRGDL 500

Query: 1783 VNAL 1794
               L
Sbjct: 501  TTLL 504


>ref|XP_002327187.1| predicted protein [Populus trichocarpa] gi|222835502|gb|EEE73937.1|
            predicted protein [Populus trichocarpa]
          Length = 510

 Score =  455 bits (1171), Expect = e-125
 Identities = 230/488 (47%), Positives = 319/488 (65%), Gaps = 8/488 (1%)
 Frame = +1

Query: 364  KNLPPSPFALPIIGHLHLFKQPLLRTVQTLSQKYGPIVFLEIGTRPVLLISSVSLVEECF 543
            +NLPP+PFALP+IGHL+L KQPL +++  L  +YGP + L  G+R V+++SS S+VEECF
Sbjct: 26   RNLPPAPFALPVIGHLYLLKQPLYKSLHALLSRYGPALSLRFGSRFVIVVSSPSVVEECF 85

Query: 544  TKNDVIFANRPQTVVADHVSYKYSTVVVSPYGPHWRNLRRITTIEIFSSKSLQTXXXXXX 723
            TKND IFANRP+++  D ++Y YS  V +PYG  WR LRR+   EIFSSKSL+       
Sbjct: 86   TKNDKIFANRPKSMAGDRLTYNYSAFVWAPYGDLWRKLRRLAVAEIFSSKSLRKSSTVRE 145

Query: 724  XXXXXLIKNLFQSYNGGLQHFDLKLMFSKLMFNVMTRMVCGKRCFEES-MDFDEQKRVHD 900
                 LI+ L +    G Q+ +L+L+FS L  NV+  +  GKRC EE       +K++  
Sbjct: 146  EEVSCLIRRLLKVSTSGTQNVELRLLFSILASNVVMIVSAGKRCVEEEHAGTKMEKQLFQ 205

Query: 901  HFKELFSPMLALGLQDFLPILRKFGLSRREKSILKLHKKRDSFLQDMINEFRKRRISSTT 1080
             FK+ F P LA+ + DF+PILR  G    EK++ KLH  RD FLQ++I+E R +   +T+
Sbjct: 206  DFKDKFFPSLAMNICDFIPILRVIGFKGLEKNMKKLHGIRDEFLQNLIDEIRLKLKKTTS 265

Query: 1081 ATAD------ESKTIIEALLSIQEADPEHYPDEFIKGIVLTMFVAGVDTMSLTLEWAFSL 1242
               D      E +++ E LL +QE++PE Y DE IK  VL MFVAG +T ++TLEWA +L
Sbjct: 266  LKTDEVTDGEERRSVAEILLCLQESEPEFYTDEVIKSTVLMMFVAGTETSAITLEWALTL 325

Query: 1243 LVNNPDKLHKARSEIDSHV-QGHVLDDSDLTNLPYLHCIIHETXXXXXXXXXXXXXXSSE 1419
            L+N+P  + K ++EID HV  G +L++SD+  LPYL C+I+ET              SSE
Sbjct: 326  LLNHPKVMQKVKAEIDEHVGHGRLLNESDIVKLPYLRCVINETLRLYPPAPLLLPHFSSE 385

Query: 1420 DCTVGGYNIPRGTILFTNIWGIHRDPKVWEEPTTFIPERFEDVHGEREMGIKFSPFGVGR 1599
             CT GG++IP+GT+L  N W +HRDPK+WEEP  F PERFE   GE + G K+ PFG+GR
Sbjct: 386  ACTAGGFDIPQGTMLVVNAWTMHRDPKLWEEPNEFKPERFEASLGEGD-GFKYIPFGIGR 444

Query: 1600 RSCPGAGMSMRMLPLALGTLIQCFEWERVGREQVEMDEHSTGVTMPKAMPLVAMCKPRSN 1779
            R CPGA M ++++ LALG L+QCFEW++VG   VE   H  G+ + KA PL A+C PR +
Sbjct: 445  RVCPGASMGLQIVSLALGVLVQCFEWDKVG--TVEDTSHGLGMILSKAKPLEALCSPRRD 502

Query: 1780 MVNALSQI 1803
            ++  LS +
Sbjct: 503  LITLLSHL 510