BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00003435
         (1890 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270570.2| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   523   e-146
ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   519   e-144
ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   488   e-135
ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A...   486   e-135
gb|ACU19018.1| unknown [Glycine max]                                  484   e-134

>ref|XP_002270570.2| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Vitis vinifera]
          Length = 387

 Score =  523 bits (1347), Expect = e-146
 Identities = 272/392 (69%), Positives = 303/392 (77%), Gaps = 1/392 (0%)
 Frame = +1

Query: 322  MEASGMDEGKMKENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTCCKHEFHLQ 501
            ME  GM+E K  ENH+TSAAAFVEGG+Q+ACDDACSICLEAFCDSDPST+T CKHEFHLQ
Sbjct: 1    MEVEGMEEVKNSENHMTSAAAFVEGGVQEACDDACSICLEAFCDSDPSTLTSCKHEFHLQ 60

Query: 502  CILEWCQRSSQCPMCWQSISLKDPTCQELLDAVEHERKLRFNPPRNATIFHHPALGEFEL 681
            CILEWCQRSSQCPMCWQSISLKDPT QELL+AVE ER +R  PPR+A IFHHP LG+FEL
Sbjct: 61   CILEWCQRSSQCPMCWQSISLKDPTSQELLEAVERERNIRLTPPRSA-IFHHPTLGDFEL 119

Query: 682  QHLPVGVNDSELEERIIQHLAAAA-MGRSHHMARREGQRSRSTAQGRPQFLVFSANPNAS 858
            QHLPVG ND ELEERIIQHLAAAA MGR+HH+ARREGQRSRS+A GR  FLVFS +PNA+
Sbjct: 120  QHLPVGTNDPELEERIIQHLAAAAAMGRAHHIARREGQRSRSSAHGRSHFLVFSTHPNAT 179

Query: 859  PTSPISSPPDQGGRNEPAXXXXXXXXXXXXXXEREETSQGIPQLLRAHTEPSPAPASGSS 1038
             + P+ +   Q G NEPA                +E SQ IPQ     T+   A AS S+
Sbjct: 180  ASGPVPASLVQVGENEPAAITVASPSIPLPAGG-DEPSQEIPQFPSVQTDHMSASASRSA 238

Query: 1039 VLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRYKESITKNTR 1218
            V      R S NNR+SASQSSP +QDRAGPSELQS SESLKSR +AVS RYKESI+K+TR
Sbjct: 239  V---TPRRISFNNRSSASQSSPPDQDRAGPSELQSFSESLKSRLNAVSMRYKESISKSTR 295

Query: 1219 GWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSGSDNLVDSAT 1398
            GWKERLFSRN SM+DLGSEVRREVNAGIASVSR+MERLDTREN+    +S S +L DS T
Sbjct: 296  GWKERLFSRNNSMADLGSEVRREVNAGIASVSRMMERLDTRENNGAGQASVSSHLADSVT 355

Query: 1399 EPDNQRSTETHVRNPLNDSNNSPSCAASSGQN 1494
            E  NQ   ET   NPLNDSN   +CAASSG N
Sbjct: 356  EQSNQNERETRAENPLNDSNTPAACAASSGSN 387


>ref|XP_002277399.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A [Vitis vinifera]
            gi|296084011|emb|CBI24399.3| unnamed protein product
            [Vitis vinifera]
          Length = 393

 Score =  519 bits (1336), Expect = e-144
 Identities = 268/393 (68%), Positives = 301/393 (76%), Gaps = 2/393 (0%)
 Frame = +1

Query: 322  MEASGMDEGKMKENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTCCKHEFHLQ 501
            MEA GM+E    E HLTSAAAFVEGGIQ+ACDDACSICLEAFCDSDPSTVT CKHEFHLQ
Sbjct: 1    MEAPGMEESNKSECHLTSAAAFVEGGIQEACDDACSICLEAFCDSDPSTVTSCKHEFHLQ 60

Query: 502  CILEWCQRSSQCPMCWQSISLKDPTCQELLDAVEHERKLRFNPPRNATIFHHPALGEFEL 681
            CILEWCQRSSQCPMCWQ ISLKDPT QELL+AVE ER  RFNP RNATIFHHP LG+FEL
Sbjct: 61   CILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERTFRFNPSRNATIFHHPTLGDFEL 120

Query: 682  QHLPVGVNDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFLVFSANPNAS 858
            QHLPVG ND+ELEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGRPQFLVFSA+P+  
Sbjct: 121  QHLPVGANDAELEERIIQHLAAAAAMGRARHIARREGQRTRSSAQGRPQFLVFSAHPSTP 180

Query: 859  PTSPISSPPDQGGRNEPAXXXXXXXXXXXXXXEREETSQGIPQLLRAHTEPSPAPASGSS 1038
               P SS P Q G +EPA                EE+S+     L +  E   A ASGS 
Sbjct: 181  QAGPASSSPAQRGEDEPAPAITAAMPSSLPTAVGEESSRLTTSQLSSQAEEITASASGSI 240

Query: 1039 VLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRYKESITKNTR 1218
            +  +NQ   S NNR S  QS P  QD+AGPSE QS SE+LKSRF+AVS RYKESI+K+TR
Sbjct: 241  IHTTNQHGTSSNNRRSPGQSLPSGQDKAGPSEFQSFSETLKSRFNAVSMRYKESISKSTR 300

Query: 1219 GWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSGSDNLVD-SA 1395
            GWKERLFSRNTSM+D+GS+VRREVNAGIA+VSR+ME L+TR+NSR T SS SD+  D S+
Sbjct: 301  GWKERLFSRNTSMADIGSDVRREVNAGIATVSRMMEHLETRDNSRTTPSSVSDSSEDTSS 360

Query: 1396 TEPDNQRSTETHVRNPLNDSNNSPSCAASSGQN 1494
                +QR  ET   NPL D+N   SCAASSG N
Sbjct: 361  PSLGDQRMAETSAHNPLGDTNVQASCAASSGTN 393


>ref|XP_003540274.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 401

 Score =  488 bits (1255), Expect = e-135
 Identities = 254/403 (63%), Positives = 295/403 (73%), Gaps = 12/403 (2%)
 Frame = +1

Query: 322  MEASGMDEGKM---------KENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 474
            ME +GM+E  +          E HLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT
Sbjct: 1    MEGAGMEENNIINDDDNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 60

Query: 475  CCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPTCQELLDAVEHERKLRFNPPRNATIFH 654
             CKHEFHLQCILEWCQRSSQCPMCWQ ISLKDPT QELL+AVE ER  RFNP RNATIFH
Sbjct: 61   SCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFH 120

Query: 655  HPALGEFELQHLPVGVNDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFL 831
            HP LG+FELQHLPVG +D++LEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGRP FL
Sbjct: 121  HPTLGDFELQHLPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFL 180

Query: 832  VFSANPNASPTSPISSPPDQGGRNEPAXXXXXXXXXXXXXXEREETSQGIPQLLRAHTEP 1011
            VFS NPN+ P +P SS P Q G +EP                 E +   +   ++A  E 
Sbjct: 181  VFSTNPNSPPMAPASSSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQA--EQ 238

Query: 1012 SPAPASGSSVLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRY 1191
              A  SGS+ LA++    S NNR S +QSSP +QDRAGPSELQS SESLKS+ +AVSTRY
Sbjct: 239  VSASGSGSTALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRY 298

Query: 1192 KESITKNTRGWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSG 1371
            KESI+K+TRGWKER FSRN +MSDLGSEVRREVNAGIA+VSR+MERL+T +N+R   +S 
Sbjct: 299  KESISKSTRGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSA 358

Query: 1372 SDNLVDSATEPDNQRSTETHVRNP--LNDSNNSPSCAASSGQN 1494
              N+ D + +  N ++          L D+N   SCAA S  N
Sbjct: 359  PSNIEDGSVQEYNGQTLTPGNEGDGLLRDNNTKASCAAGSSSN 401


>ref|XP_003543397.1| PREDICTED: E3 ubiquitin-protein ligase RHF2A-like [Glycine max]
          Length = 398

 Score =  486 bits (1251), Expect = e-135
 Identities = 255/401 (63%), Positives = 298/401 (74%), Gaps = 10/401 (2%)
 Frame = +1

Query: 322  MEASGMDEGKM------KENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTCCK 483
            ME +GM+E  +       E HLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT CK
Sbjct: 1    MEGAGMEENNIINDDNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVTSCK 60

Query: 484  HEFHLQCILEWCQRSSQCPMCWQSISLKDPTCQELLDAVEHERKLRFNPPRNATIFHHPA 663
            HEFHLQCILEWCQRSSQCPMCWQ ISLKDPT QELL+AVE ER  RFNP RNATIFHHP 
Sbjct: 61   HEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFHHPT 120

Query: 664  LGEFELQHLPVGVNDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFLVFS 840
            LG+FELQHLPVG +D++LEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGR  FLVFS
Sbjct: 121  LGDFELQHLPVGASDADLEERIIQHLAAAAAMGRARHVARREGQRNRSSAQGRQHFLVFS 180

Query: 841  ANPNASPTSPISSPPDQGGRNEPAXXXXXXXXXXXXXXEREETSQGIPQLLR-AHTEPSP 1017
             +PN+ P +P SS P Q G +EP                 EE+SQ  P L+     E   
Sbjct: 181  THPNSPPMAPASSSPSQRGDDEPT-PTIDVANLSPTPATGEESSQ--PTLVSPVQAEQVS 237

Query: 1018 APASGSSVLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRYKE 1197
            A  SG++ LA++    S NNR S +QSSP +QDRAGPSELQS SESLKS+ +AVSTRYKE
Sbjct: 238  ASGSGTTALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKE 297

Query: 1198 SITKNTRGWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSGSD 1377
            SI+K+TRGWKER FSRN +MSDLGSEVRREVNAGIA+VSR+MERL+TR+N+R   +S   
Sbjct: 298  SISKSTRGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETRDNNRSNSNSAPS 357

Query: 1378 NLVDSATEPDNQR--STETHVRNPLNDSNNSPSCAASSGQN 1494
            N+ D + +  N +  +      + + D+N   SCAA S  N
Sbjct: 358  NIEDGSVQGSNDQTLTAGNEGGSLVRDNNTKASCAAGSSSN 398


>gb|ACU19018.1| unknown [Glycine max]
          Length = 401

 Score =  484 bits (1245), Expect = e-134
 Identities = 252/403 (62%), Positives = 293/403 (72%), Gaps = 12/403 (2%)
 Frame = +1

Query: 322  MEASGMDEGKM---------KENHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 474
            ME +GM+E  +          E HLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT
Sbjct: 1    MEGAGMEENNIINDDDNNNKSEAHLTSAAAFVEGGIQDACDDACSICLEAFCDSDPSTVT 60

Query: 475  CCKHEFHLQCILEWCQRSSQCPMCWQSISLKDPTCQELLDAVEHERKLRFNPPRNATIFH 654
             CKHEFHLQCILEWCQRSSQCPMCWQ ISLKDPT QELL+AVE ER  RFNP RNATIFH
Sbjct: 61   SCKHEFHLQCILEWCQRSSQCPMCWQPISLKDPTSQELLEAVERERNFRFNPSRNATIFH 120

Query: 655  HPALGEFELQHLPVGVNDSELEERIIQHL-AAAAMGRSHHMARREGQRSRSTAQGRPQFL 831
            HP LG+FELQHLPVG +D++LEERIIQHL AAAAMGR+ H+ARREGQR+RS+AQGRP FL
Sbjct: 121  HPTLGDFELQHLPVGASDADLEERIIQHLAAAAAMGRARHIARREGQRNRSSAQGRPHFL 180

Query: 832  VFSANPNASPTSPISSPPDQGGRNEPAXXXXXXXXXXXXXXEREETSQGIPQLLRAHTEP 1011
             FS NPN+ P +P SS P Q G +EP                 E +   +   ++A  E 
Sbjct: 181  AFSTNPNSPPMAPASSSPSQRGDDEPIPTIAVANLSPTPATGEESSQLTLVPPIQA--EQ 238

Query: 1012 SPAPASGSSVLASNQSRFSINNRNSASQSSPINQDRAGPSELQSISESLKSRFSAVSTRY 1191
              A  SGS+ LA++    S NNR S +QSSP +QDRAGPSELQS SESLKS+ +AVSTRY
Sbjct: 239  VSASGSGSTALATDHQGSSYNNRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRY 298

Query: 1192 KESITKNTRGWKERLFSRNTSMSDLGSEVRREVNAGIASVSRLMERLDTRENSRVTGSSG 1371
            KESI+K+TRGWKER FSRN +MSDLGSEVRREVNAGIA+VSR+MERL+T +N+R   +S 
Sbjct: 299  KESISKSTRGWKERWFSRNNTMSDLGSEVRREVNAGIATVSRMMERLETSDNNRSNSNSA 358

Query: 1372 SDNLVDSATEPDNQRSTETHVRNP--LNDSNNSPSCAASSGQN 1494
              N+ D + +  N ++          L D+N   SCAA    N
Sbjct: 359  PSNIEDGSVQEYNGQTLTPGNEGDGLLRDNNTKASCAAGFSSN 401