BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00003074
         (1537 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   876   0.0  
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    501   e-139
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   491   e-136
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   437   e-120
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   433   e-119

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  876 bits (2263), Expect = 0.0
 Identities = 444/486 (91%), Positives = 447/486 (91%)
 Frame = -2

Query: 1494 MDLQIALFSXXXXXXXXXXXXXXXXXXXXPGPHPWPLIGNLPTLFTNTEVPLHITLANMA 1315
            MDLQIALFS                    PGPHPWPLIGNLP LFTNTEVPLHITLANMA
Sbjct: 1    MDLQIALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMA 60

Query: 1314 RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 1135
            RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC
Sbjct: 61   RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 120

Query: 1134 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIEFLRSKEGQVVKISQFVFGTLLNI 955
            TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELI+FLRSKEGQVVKISQFVFGTLLNI
Sbjct: 121  TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 180

Query: 954  LGNVVFSKDVFVYSDETDKGGIQNLIREMLMIGAEPNVAEFYPILEELDLQGLKKKCDDR 775
            LGNVVFSKDVFVY DETDKGGIQNLIREMLMIGAEPNVAEFYP LEELDLQGLKKKCD+R
Sbjct: 181  LGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDER 240

Query: 774  FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 595
            FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE
Sbjct: 241  FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 300

Query: 594  WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMXXXXXXXXXXXX 415
            WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAM            
Sbjct: 301  WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPR 360

Query: 414  XXAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIP 235
              AETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTF PERFLESDVDFHGAHYQFIP
Sbjct: 361  RAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIP 420

Query: 234  FGSGRRTCVGMPLATRTIPLIVGSLVHNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCV 55
            FGSGRRTCVGMPLATRTIPLIVGSLVHNYD GLPGGNRPEDLKMNEMLSL+LAIDPSLCV
Sbjct: 421  FGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLCV 480

Query: 54   VPKARA 37
            VPKARA
Sbjct: 481  VPKARA 486


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  501 bits (1290), Expect = e-139
 Identities = 249/346 (71%), Positives = 285/346 (82%)
 Frame = -2

Query: 1077 MLQAQSHVREQKVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDK 898
            MLQ Q HVREQKVAEL+EFL+ KEGQVVKISQ VFGTLLNILGNV+FSKDVF Y    D 
Sbjct: 1    MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVG-ADG 59

Query: 897  GGIQNLIREMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANR 718
              +Q LI+EMLMIGA PN+AEF+PI EE DLQGLKK+C+ RF  VMKMW+GTV+ERK  R
Sbjct: 60   DKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKR 119

Query: 717  NEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKE 538
            NEE KDMLDVLL NDFNDAQINA+FLETFGPGSETSSAT EW +AELIK+P  +AKVR+E
Sbjct: 120  NEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQE 179

Query: 537  LNEVVGTSTIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXAETCEVMGYTIPKNSQV 358
            L++VVG ST+KESDLP LPYLQACIKE M               ETC VMGYTIPK++QV
Sbjct: 180  LDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQV 239

Query: 357  LVNAYAIGRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIP 178
            LVN YAIGRDPK+WK+P+TF+PERFLESDVD++G H++FIPFG+GRR CVGMPLATRTIP
Sbjct: 240  LVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIP 299

Query: 177  LIVGSLVHNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 40
            LIV +LV   D  LP G  P+DL MNE LSLSLA+DP+L V+PK+R
Sbjct: 300  LIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  491 bits (1263), Expect = e-136
 Identities = 240/455 (52%), Positives = 322/455 (70%), Gaps = 1/455 (0%)
 Frame = -2

Query: 1401 PHPWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKT 1222
            P  WP++G+LP L +    P    ++N+A+ +GP++ L  G   ++ AST EAAME+L+T
Sbjct: 32   PPAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLKFGLHSSIFASTKEAAMEVLQT 91

Query: 1221 HDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQK 1042
            +D++ S R     FR+K HI YS++WSD   YWK  RKI+ TEIFS KMLQAQ   RE+ 
Sbjct: 92   NDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKILHTEIFSQKMLQAQEKNRERV 151

Query: 1041 VAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNLIREMLM 862
               L+ F+ +K G VV++  ++FG  LN+LG+VVFSKDVF YSD++D+ G+  LI  MLM
Sbjct: 152  AGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKDVFEYSDQSDEVGMDKLIHGMLM 211

Query: 861  IGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESKDMLDVLL 682
             G + +VA ++P+L   DL GLK+K D++F  ++K+WEG V  R+ANRN E KDMLDVL+
Sbjct: 212  TGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDMLDVLI 271

Query: 681  ANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVG-TSTIK 505
            ANDFN+ QINA+F+ETFGPGS+T+S  IEW +A+LIK+P ++AK+R+EL+ VVG +ST+K
Sbjct: 272  ANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVK 331

Query: 504  ESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXAETCEVMGYTIPKNSQVLVNAYAIGRDP 325
            ES   +LPYLQAC+KE M               ETC+VMGYTIPK   V VNA+AIGRDP
Sbjct: 332  ESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDP 391

Query: 324  KSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYD 145
            K WKDP  F+PERFL+SD++++G  +QFIPFGSGRR C G PLA R IPL++ SLVH + 
Sbjct: 392  KDWKDPLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFG 451

Query: 144  LGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 40
              LP G   E L M E+ +LSL +   L V+PK R
Sbjct: 452  WELPDGVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  437 bits (1123), Expect = e-120
 Identities = 225/460 (48%), Positives = 307/460 (66%), Gaps = 4/460 (0%)
 Frame = -2

Query: 1404 GPHPWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILK 1225
            GP P P++GNL  L        H   A +A+ +G +  L LG+Q  V+AS+  AA EILK
Sbjct: 32   GPRPSPIVGNLLQLGDKP----HAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILK 87

Query: 1224 THDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQ 1045
            THD+I S R++  SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS +RE 
Sbjct: 88   THDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREA 147

Query: 1044 KVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNL---IR 874
            K  E+++FLR KEG+VVKI + VFGTL+NI GN++FSKDVF   D T  GG   L   + 
Sbjct: 148  KAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPT--GGSVELKEHLW 205

Query: 873  EMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESK-DM 697
            ++L +G   N A+++PI+ +LDL G ++   +   ++  +W   +KER+  +  ESK D 
Sbjct: 206  KLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDF 265

Query: 696  LDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGT 517
            +DVLL    +D +INAL +E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG 
Sbjct: 266  VDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGD 325

Query: 516  STIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXAETCEVMGYTIPKNSQVLVNAYAI 337
            +T+KESDLP LPYLQA +KE +               ETC VM YTIPK  Q++VNA+AI
Sbjct: 326  NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAI 385

Query: 336  GRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLV 157
            GRDPK+W DP  F+PERFL SDVD+ G  ++ IPFG GRR C G+PLA++   LIV +LV
Sbjct: 386  GRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLV 445

Query: 156  HNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKARA 37
             N++  LP G    +L M+E   L+L  DP L +V KARA
Sbjct: 446  QNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARA 485


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  433 bits (1114), Expect = e-119
 Identities = 223/459 (48%), Positives = 304/459 (66%), Gaps = 4/459 (0%)
 Frame = -2

Query: 1404 GPHPWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILK 1225
            GP P P++GNL  L            A +A+ +G +  L LG+Q  V+AS+  AA E+LK
Sbjct: 32   GPRPLPIVGNLLQLGDKPRAEF----AKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLK 87

Query: 1224 THDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQ 1045
            THD+I S R++  SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS +RE 
Sbjct: 88   THDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREG 147

Query: 1044 KVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNL---IR 874
            K  E+++FLRSKEG+VVKI + VFGTL+NI GN++FSKDVF   D T  GG   L   + 
Sbjct: 148  KAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPT--GGSVELKEHLW 205

Query: 873  EMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESK-DM 697
            ++L +G   N A+++PI+ + DL G ++   D   ++  +W   +KER+  +  ES  D 
Sbjct: 206  KLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDF 265

Query: 696  LDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGT 517
            +DVLL     D +INAL +E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG 
Sbjct: 266  VDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGD 325

Query: 516  STIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXAETCEVMGYTIPKNSQVLVNAYAI 337
            +T+KESDLP LPYLQA +KE +               ETC VM YTIPK  Q++VNA+AI
Sbjct: 326  NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAI 385

Query: 336  GRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLV 157
            GRDPK+W DP  F+PERFL SDVD+ G  ++ IPFG GRR C G+PLA++ I LIV +LV
Sbjct: 386  GRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLV 445

Query: 156  HNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 40
             N++  LP G    +L M+E   L+L  DP L +V KAR
Sbjct: 446  QNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484