BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00003049
         (871 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2;...   372   e-101
sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Fl...   242   7e-62
gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]             218   2e-54
pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthas...   215   9e-54
gb|ACJ76785.1| S-norcoclaurine synthase 1 [Argemone mexicana]         150   5e-34

>sp|A2A1A1.2|NCS2_COPJA RecName: Full=S-norcoclaurine synthase 2; AltName:
           Full=Pathogenesis related protein 10A; Short=CjPR10A;
           Flags: Precursor gi|289176978|dbj|BAF45338.2|
           pathogenesis related protein 10A [Coptis japonica var.
           dissecta]
          Length = 196

 Score =  372 bits (955), Expect = e-101
 Identities = 181/185 (97%), Positives = 183/185 (98%)
 Frame = +2

Query: 14  VFLMFIGTISCERLIFNGRPLLHRVTNEETVMLYHELEVAASADEVWSVEGSPELGLHLP 193
           VFLMFIGTI+CERLIFNGRPLLHRVT EETVMLYHELEVAASADEVWSVEGSPELGLHLP
Sbjct: 9   VFLMFIGTINCERLIFNGRPLLHRVTKEETVMLYHELEVAASADEVWSVEGSPELGLHLP 68

Query: 194 DLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDGDFF 373
           DLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVE IDGDFF
Sbjct: 69  DLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEQIDGDFF 128

Query: 374 DLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEFAKIVKPLIDTVPLAIMSEAIAKVVL 553
           DLGVTYYMDTIRVVATGPDSCVIKS+TEYHVKPEFAKIVKPLIDTVPLAIMSEAIAKVVL
Sbjct: 129 DLGVTYYMDTIRVVATGPDSCVIKSTTEYHVKPEFAKIVKPLIDTVPLAIMSEAIAKVVL 188

Query: 554 ENKHK 568
           ENKHK
Sbjct: 189 ENKHK 193


>sp|Q67A25.1|NCS_THLFG RecName: Full=S-norcoclaurine synthase; Flags: Precursor
           gi|38492394|gb|AAR22502.1| (S)-norcoclaurine synthase
           [Thalictrum flavum subsp. glaucum]
           gi|226897754|gb|ACO90248.1| norcoclaurine synthase
           [Thalictrum flavum]
          Length = 210

 Score =  242 bits (618), Expect = 7e-62
 Identities = 121/190 (63%), Positives = 149/190 (78%), Gaps = 5/190 (2%)
 Frame = +2

Query: 14  VFLMFIGTISCERLIFNGRPLLHR--VTNEE---TVMLYHELEVAASADEVWSVEGSPEL 178
           VFLM +GTI+C++LI  GRP LH   + N+    T +++HELEVAASAD++W+V   P L
Sbjct: 9   VFLMLLGTINCQKLILTGRPFLHHQGIINQVSTVTKVIHHELEVAASADDIWTVYSWPGL 68

Query: 179 GLHLPDLLPAGIFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMI 358
             HLPDLLP G F K EI GDGG G+ILDMTF PG+FPH Y+EKF+  D+++R K V+MI
Sbjct: 69  AKHLPDLLP-GAFEKLEIIGDGGVGTILDMTFVPGEFPHEYKEKFILVDNEHRLKKVQMI 127

Query: 359 DGDFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKPEFAKIVKPLIDTVPLAIMSEAI 538
           +G + DLGVTYYMDTI VV TG DSCVIKSSTEYHVKPEF KIV+PLI T PLA M++AI
Sbjct: 128 EGGYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMADAI 187

Query: 539 AKVVLENKHK 568
           +K+VLE+K K
Sbjct: 188 SKLVLEHKSK 197


>gb|ACO90247.1| norcoclaurine synthase [Thalictrum flavum]
          Length = 190

 Score =  218 bits (554), Expect = 2e-54
 Identities = 109/172 (63%), Positives = 137/172 (79%), Gaps = 5/172 (2%)
 Frame = +2

Query: 68  RPLLHR--VTNEETVM---LYHELEVAASADEVWSVEGSPELGLHLPDLLPAGIFAKFEI 232
           RP L+R  + N+ + +   ++HELEVAASAD++WSV   P L  HLPDLLP G F K EI
Sbjct: 1   RPFLNRQGIINQVSTVTKGVHHELEVAASADDIWSVYSWPGLAKHLPDLLP-GAFEKLEI 59

Query: 233 TGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDGDFFDLGVTYYMDTIRV 412
            GDGG G+ILDMTF PG+FPH Y+EKF+  D+++R K V+MI+G + DLGVTYYMDTI+V
Sbjct: 60  IGDGGVGTILDMTFTPGEFPHEYKEKFILVDNEHRLKKVQMIEGGYLDLGVTYYMDTIQV 119

Query: 413 VATGPDSCVIKSSTEYHVKPEFAKIVKPLIDTVPLAIMSEAIAKVVLENKHK 568
           + TG +SCVIKSSTEYHVKPEF KIV+PLI T PLA M+EAI+K+VLE+K+K
Sbjct: 120 IPTGTNSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAMAEAISKLVLEHKYK 171


>pdb|2VNE|A Chain A, The X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927323|pdb|2VNE|B Chain B, The
           X-Ray Structure Of Norcoclaurine Synthase From
           Thalictrum Flavum gi|195927324|pdb|2VQ5|A Chain A, X-Ray
           Structure Of Norcoclaurine Synthase From Thalictrum
           Flavum In Complex With Dopamine And Hydroxybenzaldehyde
           gi|195927325|pdb|2VQ5|B Chain B, X-Ray Structure Of
           Norcoclaurine Synthase From Thalictrum Flavum In Complex
           With Dopamine And Hydroxybenzaldehyde
          Length = 201

 Score =  215 bits (548), Expect = 9e-54
 Identities = 109/179 (60%), Positives = 135/179 (75%), Gaps = 5/179 (2%)
 Frame = +2

Query: 47  ERLIFNGRPLLHR--VTNEE---TVMLYHELEVAASADEVWSVEGSPELGLHLPDLLPAG 211
           ++LI  GRP LH   + N+    T +++HELEVAASAD++W+V   P L  HLPDLLP G
Sbjct: 5   QKLILTGRPFLHHQGIINQVSTVTKVIHHELEVAASADDIWTVYSWPGLAKHLPDLLP-G 63

Query: 212 IFAKFEITGDGGEGSILDMTFPPGQFPHHYREKFVFFDHKNRYKLVEMIDGDFFDLGVTY 391
            F K EI GDGG G+ILD TF PG+FPH Y+EKF+  D+++R K V+ I+G + DLGVTY
Sbjct: 64  AFEKLEIIGDGGVGTILDXTFVPGEFPHEYKEKFILVDNEHRLKKVQXIEGGYLDLGVTY 123

Query: 392 YMDTIRVVATGPDSCVIKSSTEYHVKPEFAKIVKPLIDTVPLAIMSEAIAKVVLENKHK 568
           Y DTI VV TG DSCVIKSSTEYHVKPEF KIV+PLI T PLA  ++AI+K+VLE+K K
Sbjct: 124 YXDTIHVVPTGKDSCVIKSSTEYHVKPEFVKIVEPLITTGPLAAXADAISKLVLEHKSK 182


>gb|ACJ76785.1| S-norcoclaurine synthase 1 [Argemone mexicana]
          Length = 275

 Score =  150 bits (378), Expect = 5e-34
 Identities = 76/150 (50%), Positives = 101/150 (67%), Gaps = 1/150 (0%)
 Frame = +2

Query: 116 HELEVAASADEVWSVEGSPELGLHLPDLLPAGIFAKFE-ITGDGGEGSILDMTFPPGQFP 292
           +ELEV  SAD +W+V  SP +   L D+L  G+F K + I G+GG G++LD+ FPPG  P
Sbjct: 34  YELEVPTSADSIWAVYSSPNIPTLLRDVLLPGVFEKLDVIEGNGGVGTVLDIVFPPGAVP 93

Query: 293 HHYREKFVFFDHKNRYKLVEMIDGDFFDLGVTYYMDTIRVVATGPDSCVIKSSTEYHVKP 472
             Y+EKF+  D+K R K V MI+G   D+G TYY+D I V+A  P+SCVIKSS  Y VK 
Sbjct: 94  RCYKEKFINIDNKKRLKEVIMIEGGHLDMGCTYYLDRIHVIAKTPNSCVIKSSIIYDVKK 153

Query: 473 EFAKIVKPLIDTVPLAIMSEAIAKVVLENK 562
           E+A+ +  LI T+PL  MSE IA  VL+N+
Sbjct: 154 EYAEAMSKLITTIPLKSMSEVIANYVLKNQ 183