BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00001567
         (1832 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAQ10732.1| delta-8-sphingolipid desaturase [Anemone leveillei]    810   0.0  
gb|AAN03619.1|AF406816_1 sphingolipid long chain base delta 8 de...   810   0.0  
gb|AAQ10731.1| delta-6-fatty acid desaturase [Anemone leveillei]      702   0.0  
gb|ABP01349.1| D8-sphingolipid desaturase [Jatropha curcas]           695   0.0  
gb|ABO31111.1| sphingolipid delta-8 desaturase [Nicotiana tabacum]    690   0.0  

>gb|AAQ10732.1| delta-8-sphingolipid desaturase [Anemone leveillei]
          Length = 446

 Score =  810 bits (2093), Expect = 0.0
 Identities = 378/447 (84%), Positives = 407/447 (91%)
 Frame = -2

Query: 1741 MTEKSRREITSSELKNHNKKGDLWISIQGKVYDVTNWIKDHPGGESPLMNLAGQDVTDAF 1562
            M EK RREI+S EL  H  KGDLWISIQGKVYDV+ W+KDHPGG++PLMNLAGQDVTDAF
Sbjct: 1    MAEK-RREISSQELAQHTNKGDLWISIQGKVYDVSEWVKDHPGGDAPLMNLAGQDVTDAF 59

Query: 1561 VAFHPGSAWKYLDKFFIGYLEDYKVSEVSKDYRKLVTEFSKAGLFEKKGHWICISLVTSA 1382
            VAFHPGSAWKYLDKF IGYL DY VS+VSKDYRKL +EFSKAGLFEKKGH I +SL++  
Sbjct: 60   VAFHPGSAWKYLDKFHIGYLSDYVVSDVSKDYRKLASEFSKAGLFEKKGHGIFMSLLSVG 119

Query: 1381 FLFTLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTG 1202
             +F + + GVL S   WVH   A L+G+LWMQIGFVGHDSGHYN+M+TPKLNRF+QIFTG
Sbjct: 120  IMFVVILSGVLLSGNTWVHAACAGLLGILWMQIGFVGHDSGHYNVMLTPKLNRFVQIFTG 179

Query: 1201 NCVTGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDS 1022
            NCVTGISIGWW+WTHTAHHIAVNSLD+DPDLQH+PFLAVS DI  SL S FYGRKMTFD+
Sbjct: 180  NCVTGISIGWWRWTHTAHHIAVNSLDYDPDLQHVPFLAVSKDILSSLTSKFYGRKMTFDA 239

Query: 1021 VARFLVSYQHWSFYPVMAVARINLFTQSFLLLLSKRQVPHRYLELAGLMVFWGWYSLVIS 842
             ARFLVSYQHWSFYPVMAVARINLFTQSFLLLLS R +  RYLEL GLMVFWGWYSL++S
Sbjct: 240  AARFLVSYQHWSFYPVMAVARINLFTQSFLLLLSSRPMQDRYLELLGLMVFWGWYSLLVS 299

Query: 841  CLPNWGERALFVAVSFAVSGIQHVQFCLNHFSAHTYIGPPRANDWFEKQTKGSIDISCST 662
            CLPNWGERA+FVAVSFAVSGIQHVQFCLNHFSAHTY+GPPRANDWFEKQTKGSIDISCST
Sbjct: 300  CLPNWGERAMFVAVSFAVSGIQHVQFCLNHFSAHTYVGPPRANDWFEKQTKGSIDISCST 359

Query: 661  WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANKLTIATL 482
            WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANK+TIATL
Sbjct: 360  WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANKMTIATL 419

Query: 481  RNAALQARDLTNPIPKNLLWEAVNTHG 401
            RNAALQARDLTNPIPKNL+WEA NTHG
Sbjct: 420  RNAALQARDLTNPIPKNLVWEAFNTHG 446


>gb|AAN03619.1|AF406816_1 sphingolipid long chain base delta 8 desaturase [Aquilegia vulgaris]
          Length = 446

 Score =  810 bits (2093), Expect = 0.0
 Identities = 373/447 (83%), Positives = 407/447 (91%)
 Frame = -2

Query: 1741 MTEKSRREITSSELKNHNKKGDLWISIQGKVYDVTNWIKDHPGGESPLMNLAGQDVTDAF 1562
            MTEK RREITS ELK HNK GD+WISIQGK+YDV+ WIKDHPGGE+PL+NLAGQDVTDAF
Sbjct: 1    MTEK-RREITSEELKKHNKHGDIWISIQGKIYDVSEWIKDHPGGEAPLLNLAGQDVTDAF 59

Query: 1561 VAFHPGSAWKYLDKFFIGYLEDYKVSEVSKDYRKLVTEFSKAGLFEKKGHWICISLVTSA 1382
            VAFHPGSAWKYLDKFFIGYL+DY +SEVSKDYRKLV EFSKAGL++KKGH I  SL    
Sbjct: 60   VAFHPGSAWKYLDKFFIGYLKDYTISEVSKDYRKLVAEFSKAGLYDKKGHHILFSLTFVT 119

Query: 1381 FLFTLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTG 1202
             L  +SV+GVL SDK W H+ SA ++GLLWMQIGFVGHDSGHYNIM+TPKLNRFMQIFTG
Sbjct: 120  ILMAISVMGVLCSDKTWAHLASAAVLGLLWMQIGFVGHDSGHYNIMLTPKLNRFMQIFTG 179

Query: 1201 NCVTGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDS 1022
            NC+TGISIGWWKW H AHHIAVNSLD+DPDLQHIPFLAVSSDIF SL S FYGRKMTFD 
Sbjct: 180  NCITGISIGWWKWNHNAHHIAVNSLDYDPDLQHIPFLAVSSDIFSSLTSKFYGRKMTFDP 239

Query: 1021 VARFLVSYQHWSFYPVMAVARINLFTQSFLLLLSKRQVPHRYLELAGLMVFWGWYSLVIS 842
            +ARFL+S+QHW+FYPVMA+ARINLF QSF+LLLSKR V  R LEL GLMVFW WYSL+++
Sbjct: 240  IARFLISFQHWTFYPVMAIARINLFAQSFILLLSKRPVTDRALELLGLMVFWCWYSLLLA 299

Query: 841  CLPNWGERALFVAVSFAVSGIQHVQFCLNHFSAHTYIGPPRANDWFEKQTKGSIDISCST 662
            CLPNWGERA++VA+SFAVSG QH+QFCLNHFSAHTY+GPP  NDWFEKQTKG  DISCST
Sbjct: 300  CLPNWGERAMYVAMSFAVSGYQHIQFCLNHFSAHTYVGPPPCNDWFEKQTKGPFDISCST 359

Query: 661  WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANKLTIATL 482
            WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPY+SVSFFEANK+TIATL
Sbjct: 360  WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYISVSFFEANKMTIATL 419

Query: 481  RNAALQARDLTNPIPKNLLWEAVNTHG 401
            RNAALQARDLTNPIPKNL+WEAVNTHG
Sbjct: 420  RNAALQARDLTNPIPKNLVWEAVNTHG 446


>gb|AAQ10731.1| delta-6-fatty acid desaturase [Anemone leveillei]
          Length = 446

 Score =  702 bits (1813), Expect = 0.0
 Identities = 324/447 (72%), Positives = 385/447 (86%)
 Frame = -2

Query: 1741 MTEKSRREITSSELKNHNKKGDLWISIQGKVYDVTNWIKDHPGGESPLMNLAGQDVTDAF 1562
            M EK RR I+S +L++HNK GD+WISIQGK+YDVT W KDHPGGE PL+NLAGQDVTDAF
Sbjct: 1    MAEK-RRSISSDDLRSHNKPGDVWISIQGKIYDVTEWGKDHPGGEGPLLNLAGQDVTDAF 59

Query: 1561 VAFHPGSAWKYLDKFFIGYLEDYKVSEVSKDYRKLVTEFSKAGLFEKKGHWICISLVTSA 1382
            VAFHPGSAWK LDKF IGYL+DY VS+VSKDYRKLV+EFSKAGL+EKKGH   I L+  +
Sbjct: 60   VAFHPGSAWKNLDKFHIGYLQDYVVSDVSKDYRKLVSEFSKAGLYEKKGHGHLIRLLVMS 119

Query: 1381 FLFTLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTG 1202
             +F  SV GV+ SDK  VH+ SA L+ ++WMQ GF+GHDSGHYNIM +P+LNR+MQIF+ 
Sbjct: 120  LVFIASVSGVVLSDKTSVHVGSAVLLAVIWMQFGFIGHDSGHYNIMTSPELNRYMQIFSV 179

Query: 1201 NCVTGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDS 1022
            N V+G+S+GWWK  H AHHIAVNSL++DPDLQ++PFL VS+ IF SL S FY +KMTFD+
Sbjct: 180  NVVSGVSVGWWKRYHNAHHIAVNSLEYDPDLQYVPFLVVSTAIFDSLTSHFYRKKMTFDA 239

Query: 1021 VARFLVSYQHWSFYPVMAVARINLFTQSFLLLLSKRQVPHRYLELAGLMVFWGWYSLVIS 842
            VARFLVS+QHW+FYP+MA+ R++   QS  +LLSK+ +P R+LE  GL+VFW WYSL+IS
Sbjct: 240  VARFLVSFQHWTFYPLMAIGRVSFLAQSIGVLLSKKPLPDRHLEWFGLVVFWAWYSLLIS 299

Query: 841  CLPNWGERALFVAVSFAVSGIQHVQFCLNHFSAHTYIGPPRANDWFEKQTKGSIDISCST 662
            CLPNW ER +F+AV+FAV+GIQHVQFCLNH+SA TYIG P ANDWFEKQTKGSIDISCS 
Sbjct: 300  CLPNWWERVIFIAVNFAVTGIQHVQFCLNHYSAQTYIGAPCANDWFEKQTKGSIDISCSP 359

Query: 661  WMDWFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANKLTIATL 482
            W DWFHGGLQFQ+EHHLFPR+PRC+LRKISPFVKELCRKHNL Y SVSFFE N+ T+ATL
Sbjct: 360  WTDWFHGGLQFQIEHHLFPRMPRCNLRKISPFVKELCRKHNLVYTSVSFFEGNRRTLATL 419

Query: 481  RNAALQARDLTNPIPKNLLWEAVNTHG 401
            +NAAL+ARDLT+PIPKNL+WEAV+THG
Sbjct: 420  KNAALKARDLTSPIPKNLVWEAVHTHG 446


>gb|ABP01349.1| D8-sphingolipid desaturase [Jatropha curcas]
          Length = 447

 Score =  695 bits (1794), Expect = 0.0
 Identities = 312/444 (70%), Positives = 373/444 (84%), Gaps = 1/444 (0%)
 Frame = -2

Query: 1729 SRREITSSELKNHNKKGDLWISIQGKVYDVTNWIKDHPGGESPLMNLAGQDVTDAFVAFH 1550
            S++ I+  ELK H K GDLWISIQGKVY+VT W KDHPGGE PL++L+GQDVTDAFVA+H
Sbjct: 4    SKKYISEEELKKHTKSGDLWISIQGKVYNVTGWTKDHPGGELPLLHLSGQDVTDAFVAYH 63

Query: 1549 PGSAWKYLDKFFIGY-LEDYKVSEVSKDYRKLVTEFSKAGLFEKKGHWICISLVTSAFLF 1373
            PG+AW+YLDKFF GY L+D+ VSEVSKDYRKL+ EF+K GLFEKKGH   ++L   A L 
Sbjct: 64   PGTAWQYLDKFFTGYYLKDHSVSEVSKDYRKLLAEFTKMGLFEKKGHVTLVTLCVVAMLL 123

Query: 1372 TLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTGNCV 1193
            + S+ G+L SD  WVH++S  L+G LW+Q G++GHDSGHY +M +PK+NR +QI TGNC+
Sbjct: 124  SFSIYGILCSDSTWVHLLSGGLIGFLWIQSGWIGHDSGHYQVMTSPKINRLVQILTGNCL 183

Query: 1192 TGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDSVAR 1013
             GISI WWKW H  HHIA NSL+FDPDLQH+PF AVSS +F SL+S FY RKM FDSV R
Sbjct: 184  AGISIAWWKWNHNTHHIACNSLEFDPDLQHMPFFAVSSKLFSSLKSYFYERKMNFDSVCR 243

Query: 1012 FLVSYQHWSFYPVMAVARINLFTQSFLLLLSKRQVPHRYLELAGLMVFWGWYSLVISCLP 833
            FLVSYQHW+FYPVM  AR+NLF QSFLLLLSKR+VP+R  E+ G++VFW WY L++SCLP
Sbjct: 244  FLVSYQHWTFYPVMCFARLNLFAQSFLLLLSKRRVPNRGQEILGVLVFWIWYPLLVSCLP 303

Query: 832  NWGERALFVAVSFAVSGIQHVQFCLNHFSAHTYIGPPRANDWFEKQTKGSIDISCSTWMD 653
            NWGER +FV  SFAV+GIQHVQFCLNHFS++ Y+G P  NDWFEKQTKG+++I+CS+WMD
Sbjct: 304  NWGERVMFVLASFAVTGIQHVQFCLNHFSSNVYVGLPSGNDWFEKQTKGTLNINCSSWMD 363

Query: 652  WFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANKLTIATLRNA 473
            WFHGGLQFQ+EHHLFPRLPRC LRKISPFVKELC+KH+LPY S SF++AN +TI TL  A
Sbjct: 364  WFHGGLQFQIEHHLFPRLPRCQLRKISPFVKELCKKHHLPYDSASFWKANVMTIRTLSAA 423

Query: 472  ALQARDLTNPIPKNLLWEAVNTHG 401
            ALQARDL NP+PKNL+WEAVNTHG
Sbjct: 424  ALQARDLANPVPKNLVWEAVNTHG 447


>gb|ABO31111.1| sphingolipid delta-8 desaturase [Nicotiana tabacum]
          Length = 447

 Score =  690 bits (1781), Expect = 0.0
 Identities = 312/444 (70%), Positives = 369/444 (83%), Gaps = 1/444 (0%)
 Frame = -2

Query: 1729 SRREITSSELKNHNKKGDLWISIQGKVYDVTNWIKDHPGGESPLMNLAGQDVTDAFVAFH 1550
            SR+ ITS ELKNHNK GDLWISIQGKVYDV++WIK+HPGG+ PL+NLAGQDVTDAFVA+H
Sbjct: 4    SRKYITSEELKNHNKPGDLWISIQGKVYDVSDWIKEHPGGDFPLLNLAGQDVTDAFVAYH 63

Query: 1549 PGSAWKYLDKFFIG-YLEDYKVSEVSKDYRKLVTEFSKAGLFEKKGHWICISLVTSAFLF 1373
            P +AW+YLDKFF G YL+DY  SEVSKDYR+LV+EF+K GLFEKKGH    ++ + A LF
Sbjct: 64   PATAWQYLDKFFNGFYLKDYSASEVSKDYRRLVSEFTKLGLFEKKGHVCFYTMCSIAMLF 123

Query: 1372 TLSVLGVLWSDKIWVHIISATLMGLLWMQIGFVGHDSGHYNIMMTPKLNRFMQIFTGNCV 1193
             +SV G++ S+ + VH++S  LMG LW+Q G++GHDSGHY +M T  LNRF Q+ TGNC+
Sbjct: 124  AVSVYGIMCSESVLVHLMSGGLMGFLWIQSGWIGHDSGHYQVMSTRGLNRFAQVLTGNCL 183

Query: 1192 TGISIGWWKWTHTAHHIAVNSLDFDPDLQHIPFLAVSSDIFKSLESVFYGRKMTFDSVAR 1013
             GISI WWKW H AHHIAVNSL++DPDLQH+PF  VSS  F SL S FY RKMTFDS  R
Sbjct: 184  AGISIAWWKWNHNAHHIAVNSLEYDPDLQHMPFFVVSSKFFNSLTSYFYDRKMTFDSFTR 243

Query: 1012 FLVSYQHWSFYPVMAVARINLFTQSFLLLLSKRQVPHRYLELAGLMVFWGWYSLVISCLP 833
            FLVS QHW+FYPVM +ARINLF QSF+LLLSK+ VP+R  EL G++ FW WY L++SCLP
Sbjct: 244  FLVSNQHWTFYPVMCLARINLFAQSFILLLSKKNVPYRVQELLGVVAFWIWYPLLVSCLP 303

Query: 832  NWGERALFVAVSFAVSGIQHVQFCLNHFSAHTYIGPPRANDWFEKQTKGSIDISCSTWMD 653
            NWGER +FV  SF V+GIQHVQFCLNHFS+  Y+ PP+ NDWFEKQT GS+DISC +WMD
Sbjct: 304  NWGERIMFVLASFTVTGIQHVQFCLNHFSSDIYVAPPKGNDWFEKQTGGSLDISCPSWMD 363

Query: 652  WFHGGLQFQVEHHLFPRLPRCHLRKISPFVKELCRKHNLPYVSVSFFEANKLTIATLRNA 473
            WF+GGLQFQ+EHHLFPRLPRC LRK+SPFVK+LC+KH LPY   SF+ AN LTI+TLR A
Sbjct: 364  WFYGGLQFQIEHHLFPRLPRCQLRKVSPFVKDLCKKHGLPYNCASFYMANALTISTLRTA 423

Query: 472  ALQARDLTNPIPKNLLWEAVNTHG 401
            ALQARDLT P+PKNL+WEAVNTHG
Sbjct: 424  ALQARDLTKPVPKNLVWEAVNTHG 447