BLASTX nr result
ID: Coptis24_contig00001149
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00001149
(1542 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 811 0.0
emb|CBI20743.3| unnamed protein product [Vitis vinifera] 771 0.0
ref|XP_003603241.1| 3beta-hydroxysteroid-dehydrogenase/decarboxy... 743 0.0
ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylas... 738 0.0
ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 737 0.0
>ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Vitis vinifera]
Length = 563
Score = 811 bits (2096), Expect = 0.0
Identities = 398/511 (77%), Positives = 459/511 (89%)
Frame = +3
Query: 6 MTGEEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSG 185
M GE+K CVVTGGRGFAARHLVEMLI ++ V+IADLGPSI+LEP EE+ +L +AL+SG
Sbjct: 1 MEGEQKWCVVTGGRGFAARHLVEMLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSG 60
Query: 186 AAKYVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECK 365
A+YVSADLR K+Q++KA +GA+ VFHMAAPDSSINN QLH+SVNVQGTKNVIDAC+E K
Sbjct: 61 RAEYVSADLRDKAQVLKACQGAEAVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQK 120
Query: 366 VKRLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTC 545
VKRLIYTSSPSVVFDGVHGIFNGDESL YP KHNDSYSATKAEGEALVIKSNG NGLLTC
Sbjct: 121 VKRLIYTSSPSVVFDGVHGIFNGDESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTC 180
Query: 546 CIRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSS 725
CIRPSSIFGPGD+LLVPSLV AARAGKSKFIIGDGNN+YDFTYVENVAHAH+CAE AL+S
Sbjct: 181 CIRPSSIFGPGDKLLVPSLVNAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALAS 240
Query: 726 EGEVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYK 905
EG++A++AAGQAYFITNMEPIKFWEFVS ILEGLGYERP IKIP VMMPIAH+VEWTYK
Sbjct: 241 EGKIAEQAAGQAYFITNMEPIKFWEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYK 300
Query: 906 VLAPYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAE 1085
+LAPYGM+VPQLTPSRIRLLS SRTFSCSKA+D+L Y PIVSLQ+GL+RTI+SY LRAE
Sbjct: 301 MLAPYGMKVPQLTPSRIRLLSCSRTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAE 360
Query: 1086 QKQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQI 1265
+R GPSKAS YLG G++A+TLLWKDKK LT ++VL+AFY FIASGYTI+TAVS++
Sbjct: 361 HLPKREGPSKASRYLGSGRVADTLLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKL 420
Query: 1266 LLMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLC 1445
L+A++FLF+HG LP K+LGYT+EKIP SNFHF E+ SH+ ALS S+WN AVNVLKSLC
Sbjct: 421 FLVASIFLFIHGNLPEKILGYTVEKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLC 480
Query: 1446 KGEDFVLFVKVVISLLICSFLGATSIRSIFM 1538
KG+D++LF+KVV+SLLI SFLGA S++S+F+
Sbjct: 481 KGDDWMLFLKVVLSLLILSFLGAISLQSLFV 511
>emb|CBI20743.3| unnamed protein product [Vitis vinifera]
Length = 540
Score = 771 bits (1991), Expect = 0.0
Identities = 378/488 (77%), Positives = 438/488 (89%)
Frame = +3
Query: 75 MLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGAAKYVSADLRHKSQLIKAFEGAD 254
MLI ++ V+IADLGPSI+LEP EE+ +L +AL+SG A+YVSADLR K+Q++KA +GA+
Sbjct: 1 MLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAE 60
Query: 255 VVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKRLIYTSSPSVVFDGVHGIFNG 434
VFHMAAPDSSINN QLH+SVNVQGTKNVIDAC+E KVKRLIYTSSPSVVFDGVHGIFNG
Sbjct: 61 AVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNG 120
Query: 435 DESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCCIRPSSIFGPGDRLLVPSLVTAA 614
DESL YP KHNDSYSATKAEGEALVIKSNG NGLLTCCIRPSSIFGPGD+LLVPSLV AA
Sbjct: 121 DESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAA 180
Query: 615 RAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGEVAQRAAGQAYFITNMEPIKF 794
RAGKSKFIIGDGNN+YDFTYVENVAHAH+CAE AL+SEG++A++AAGQAYFITNMEPIKF
Sbjct: 181 RAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKF 240
Query: 795 WEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLAPYGMRVPQLTPSRIRLLSRS 974
WEFVS ILEGLGYERP IKIP VMMPIAH+VEWTYK+LAPYGM+VPQLTPSRIRLLS S
Sbjct: 241 WEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCS 300
Query: 975 RTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQKQRRVGPSKASIYLGRGKIANT 1154
RTFSCSKA+D+L Y PIVSLQ+GL+RTI+SY LRAE +R GPSKAS YLG G++A+T
Sbjct: 301 RTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAEHLPKREGPSKASRYLGSGRVADT 360
Query: 1155 LLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQILLMATLFLFVHGILPAKVLGYTI 1334
LLWKDKK LT ++VL+AFY FIASGYTI+TAVS++ L+A++FLF+HG LP K+LGYT+
Sbjct: 361 LLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTV 420
Query: 1335 EKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCKGEDFVLFVKVVISLLICSFLGA 1514
EKIP SNFHF E+ SH+ ALS S+WN AVNVLKSLCKG+D++LF+KVV+SLLI SFLGA
Sbjct: 421 EKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFLKVVLSLLILSFLGA 480
Query: 1515 TSIRSIFM 1538
S++S+F+
Sbjct: 481 ISLQSLFV 488
>ref|XP_003603241.1| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform [Medicago
truncatula] gi|355492289|gb|AES73492.1|
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
[Medicago truncatula]
Length = 579
Score = 743 bits (1917), Expect = 0.0
Identities = 361/511 (70%), Positives = 435/511 (85%), Gaps = 2/511 (0%)
Frame = +3
Query: 15 EEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGAAK 194
EE CVVTGGRGFAARHLVEMLI + V+IADLG +I+LEP E+ +L QAL SG A+
Sbjct: 5 EEGWCVVTGGRGFAARHLVEMLIQLNTYCVRIADLGSTIELEPSEQLGLLGQALHSGRAQ 64
Query: 195 YVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKR 374
YVS DLR+K QL+KAF+G +VVFHMAAP+SSINN QLH+SVNV+GTKNVIDACVE KVKR
Sbjct: 65 YVSVDLRNKPQLLKAFDGVEVVFHMAAPNSSINNYQLHHSVNVEGTKNVIDACVELKVKR 124
Query: 375 LIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCCIR 554
LIYTSSPSVVFDG+HGI NG ESL YP HND YSATKAEGE LVIK+NG +GLLTCCIR
Sbjct: 125 LIYTSSPSVVFDGIHGIHNGSESLPYPPSHNDHYSATKAEGEGLVIKANGTSGLLTCCIR 184
Query: 555 PSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGE 734
PSSIFGPGD+LLVPSLV AA+AGKSKFI+GDGNN+YDFTYVENVAHAH+CA+ AL+SEG
Sbjct: 185 PSSIFGPGDKLLVPSLVDAAKAGKSKFIVGDGNNVYDFTYVENVAHAHICADRALASEGT 244
Query: 735 VAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLA 914
V+++AAG+AYFITNMEPIKFWEF+S ILEGLGY+RPSIKIPV V+MPIAH+VEW Y++L
Sbjct: 245 VSEKAAGEAYFITNMEPIKFWEFMSLILEGLGYQRPSIKIPVFVIMPIAHLVEWIYRLLG 304
Query: 915 PYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQ-- 1088
PYGM+VPQLTPSRIRL S +R+F CSKA+DRL Y PI+ LQ+G++RTIESY HLRAE
Sbjct: 305 PYGMKVPQLTPSRIRLTSCTRSFDCSKAKDRLDYAPIIPLQEGIRRTIESYPHLRAENQL 364
Query: 1089 KQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQIL 1268
K +R GPSKAS+YLG G++A+TLLWKDKKQT T ++V IA Y FIAS T ITA++++L
Sbjct: 365 KSKREGPSKASVYLGSGRVADTLLWKDKKQTFTTLLVFIAIYVNFIASENTFITALTKLL 424
Query: 1269 LMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCK 1448
L +++ LF+HGILPAK+LGYT+EK+P S FH E S+Q+A S S+WN+AVN LKSL +
Sbjct: 425 LYSSIGLFIHGILPAKILGYTVEKMPTSWFHLSEDISNQIAFSVASSWNFAVNALKSLAE 484
Query: 1449 GEDFVLFVKVVISLLICSFLGATSIRSIFMI 1541
G +V+F KVV +LL+ SFLGA S+++++ I
Sbjct: 485 GNSWVMFFKVVFTLLVLSFLGAFSLKNLYTI 515
>ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
[Arabidopsis thaliana]
gi|229890388|sp|Q67ZE1.2|HSDD2_ARATH RecName:
Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 2; Short=At3BETAHSD/D2; AltName:
Full=4alpha-carboxysterol-C3-dehydrogenase/C4-
decarboxylase isoform 1-2; AltName: Full=Reticulon-like
protein B19; Short=AtRTNLB19; AltName:
Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 2,
decarboxylating gi|330252720|gb|AEC07814.1|
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2 [Arabidopsis thaliana]
Length = 564
Score = 738 bits (1906), Expect = 0.0
Identities = 361/511 (70%), Positives = 430/511 (84%)
Frame = +3
Query: 9 TGEEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGA 188
T E+ CVVTGGRGFAARHLVEML+ + + V+IADL P+I L+P E +L + LRSG
Sbjct: 6 TETERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGR 65
Query: 189 AKYVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKV 368
+Y+SADLR KSQ++KAF+GA+VVFHMAAPDSSINN QL YSVNVQGT+NVIDACV+ V
Sbjct: 66 VQYISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGV 125
Query: 369 KRLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCC 548
KRLIYTSSPSVVFDGVHGI NG ES+AYP KHNDSYSATKAEGE L++K+NGRNGLLTCC
Sbjct: 126 KRLIYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCC 185
Query: 549 IRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSE 728
IRPSSIFGPGDRLLVPSLV AARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAE AL+S
Sbjct: 186 IRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASG 245
Query: 729 GEVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKV 908
G+V+ +AAGQAYFITNMEPIKFWEF+S++L+GLGYERPSIKIP +MMPIAH+VE TYKV
Sbjct: 246 GDVSTKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKV 305
Query: 909 LAPYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQ 1088
L PYGM VPQLTPSR+RLLS SRTF +KA+DRLGY P+V LQ+G++RTI+S+SHL A
Sbjct: 306 LGPYGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGS 365
Query: 1089 KQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQIL 1268
+ +R GPSKAS LG GK+A+TLLWKD KQTL A+ +LI+ Y F+A+G T++TA+S+ L
Sbjct: 366 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 425
Query: 1269 LMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCK 1448
L+A++FLF+HGILP K+ GYT+EKIPAS FH + +SH ++LS S+WN V L+SLC+
Sbjct: 426 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 485
Query: 1449 GEDFVLFVKVVISLLICSFLGATSIRSIFMI 1541
G D+ F KVV LL S GA S+ SIF+I
Sbjct: 486 GNDWSFFFKVVFVLLALSLAGAISLHSIFVI 516
>ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Glycine max]
Length = 563
Score = 737 bits (1903), Expect = 0.0
Identities = 367/511 (71%), Positives = 432/511 (84%), Gaps = 2/511 (0%)
Frame = +3
Query: 15 EEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGAAK 194
E+K CVVTGGRGFAARHLVEMLI ++ V+IADL +I LEP E+ +L QAL SG A+
Sbjct: 5 EDKWCVVTGGRGFAARHLVEMLIRHNEYCVRIADLEANIVLEPAEQLGLLGQALHSGRAQ 64
Query: 195 YVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKR 374
YVS DLR+K+QL+KA EG +VVFHMAAP+SSINN QLH+SVNVQGTKNVIDACVE VKR
Sbjct: 65 YVSLDLRNKAQLLKALEGVEVVFHMAAPNSSINNYQLHHSVNVQGTKNVIDACVELNVKR 124
Query: 375 LIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCCIR 554
L+YTSSPSVVFDGVHGI NG+E++ Y ND YSATKAEGEALVIK+NG NGLLTCCIR
Sbjct: 125 LVYTSSPSVVFDGVHGIHNGNETMPYAHSPNDHYSATKAEGEALVIKANGTNGLLTCCIR 184
Query: 555 PSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGE 734
PSSIFGPGDRLLVPSLV AAR GKSKF+IGDGNN+YDFTYVENVAHAH+CA+ AL SE
Sbjct: 185 PSSIFGPGDRLLVPSLVDAARKGKSKFLIGDGNNVYDFTYVENVAHAHICADRALVSEAP 244
Query: 735 VAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLA 914
V+++AAG+AYFITNMEP+KFWEFVS ++EGLGYE P IKIP V+MP AH+VEW Y++L
Sbjct: 245 VSEKAAGEAYFITNMEPMKFWEFVSVVVEGLGYEGPRIKIPTFVIMPFAHLVEWIYRLLG 304
Query: 915 PYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQ-- 1088
PYGM+VPQLTPSRIRL S SRTF CSKA+DRLGY PIV+LQ+GL+RTIESY+HL+AE
Sbjct: 305 PYGMKVPQLTPSRIRLTSCSRTFDCSKAKDRLGYAPIVTLQEGLRRTIESYTHLKAENQP 364
Query: 1089 KQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQIL 1268
K +R G SKASIYLG G++A+TLLWKDKKQT A++VLIA Y FIAS TII+A++++L
Sbjct: 365 KTKREGSSKASIYLGSGRVADTLLWKDKKQTFIALLVLIAIYFNFIASENTIISALTKLL 424
Query: 1269 LMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCK 1448
L A++FLF+HGILPAK+LGYT+EKIP S FH E SHQ+ALS S+WN AVNVLKSL
Sbjct: 425 LFASIFLFIHGILPAKILGYTLEKIPKSWFHLSEDMSHQIALSVASSWNIAVNVLKSLAD 484
Query: 1449 GEDFVLFVKVVISLLICSFLGATSIRSIFMI 1541
G D+VLF K V+SL I SFLGA S++S++ I
Sbjct: 485 GNDWVLFFKAVLSLFILSFLGAFSLQSLYTI 515