BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00000611
         (2029 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   674   0.0  
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        278   5e-72
ref|XP_004145402.1| PREDICTED: RING-H2 finger protein ATL65-like...   262   3e-67
ref|XP_002281140.2| PREDICTED: RING-H2 finger protein ATL65-like...   261   7e-67
gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papave...   256   2e-65

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT; AltName:
            Full=Tetrahydrocolumbamine 2-O-methyltransferase
            gi|24210466|dbj|BAC22084.1| columbamine
            O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  674 bits (1739), Expect = 0.0
 Identities = 335/351 (95%), Positives = 344/351 (98%)
 Frame = +3

Query: 57   MDTPNTFQNDDEIKAQAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNGPVTLSQLVTHLP 236
            MDTPNTFQNDDEIKAQA+VWKHM+GFA+TIMLRST+SLGIPDIIHNNGPVTLSQLVTHLP
Sbjct: 1    MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 60

Query: 237  LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 416
            LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT
Sbjct: 61   LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 120

Query: 417  HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEMNEILNDAMTSHSKFMLPALVS 596
            HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPE+NEILNDAMTSHS FMLPALVS
Sbjct: 121  HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVS 180

Query: 597  GLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIESVIKDPKLDYVAG 776
            GLMKENVLDGVASIVDVGGNSGVVAK IVDAFP VKCSVMDLNHVIE VIK+PKLDYVAG
Sbjct: 181  GLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 240

Query: 777  DMFTSVPNADAILLKSTLHNYEDDDCIKILNKAKEALPSTGGKVILVEIVVDTENLPLFT 956
            DMFTS+PNADAILLKSTLHNYEDDDCIKILN AKEALPSTGGKVILVEIVVDTENLPLFT
Sbjct: 241  DMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFT 300

Query: 957  SARLSMDVDMMLMSGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAYS 1109
            SARLSM +DMMLMSGKERTKKEWEDLLRKA+FT HQVIPIMAIESIIVAYS
Sbjct: 301  SARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAYS 351


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  278 bits (710), Expect = 5e-72
 Identities = 137/338 (40%), Positives = 221/338 (65%), Gaps = 3/338 (0%)
 Frame = +3

Query: 102  QAEVWKHMYGFAKTIMLRSTISLGIPDIIHNNG-PVTLSQLVTHLPLKSTSIDRFHHFMR 278
            QA++WKH+YGFA++++L+S + L I D IHN+G P+TLS+L + LP++   +DR +  MR
Sbjct: 14   QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73

Query: 279  YLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVWSHVIN- 455
            YLVHM+LF    D+  +  KY L P +K L+ G +KS+   ++L    +  S W H+   
Sbjct: 74   YLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKEG 133

Query: 456  VLDGKKPYWESNDTSMYEKTEGDPEMNEILNDAMTSHSKFMLPALVSGLMKENVLDGVAS 635
            +++    + ++   S+++    +P+ N++ N+ M   S+ +  ALV+     N+  G+ +
Sbjct: 134  LVNNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNEC--GNIFQGIQT 191

Query: 636  IVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIESVIKDPKLDYVAGDMFTSVPNADAIL 815
            +VDVGG +G   KA+  AFP +KCS+ DL HVI      P +  + G+MF S+P+ADAIL
Sbjct: 192  LVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSADAIL 251

Query: 816  LKSTLHNYEDDDCIKILNKAKEALPSTGGKVILVEIVVDTENLPLFTSARLSMDVDMML- 992
            +K  LH++ DD+CI+IL K KEA+P  GGKVI+V+IV+D E+   ++  RL++D+DMML 
Sbjct: 252  MKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMMLN 311

Query: 993  MSGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 1106
              GKERT++EW+ L+  A F  +++  I A++S+I A+
Sbjct: 312  TEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAF 349


>ref|XP_004145402.1| PREDICTED: RING-H2 finger protein ATL65-like [Cucumis sativus]
            gi|449472703|ref|XP_004153673.1| PREDICTED: RING-H2
            finger protein ATL65-like [Cucumis sativus]
            gi|449532533|ref|XP_004173235.1| PREDICTED: RING-H2
            finger protein ATL65-like [Cucumis sativus]
          Length = 421

 Score =  262 bits (669), Expect = 3e-67
 Identities = 159/261 (60%), Positives = 191/261 (73%), Gaps = 29/261 (11%)
 Frame = -1

Query: 2008 DCAVCLVEFEENDYVRTLPICSHAFHVECIDIWLTTHANCPLCRAGIFRLES----LMAS 1841
            +CAVCL+EFE++DYVRTLPICSHAFHV+CID+WL +HANCPLCRA +FR ES    +MA+
Sbjct: 164  ECAVCLLEFEDDDYVRTLPICSHAFHVDCIDVWLRSHANCPLCRAVVFRPESPFTPVMAA 223

Query: 1840 RIRPSLDG-ILESFLSETRSV-----------IQETEIS---DQVSSQRITETEERSRNN 1706
            RIRPSLD  IL S + E  +              E+EI+   D+  +    E +   R+ 
Sbjct: 224  RIRPSLDDTILRSIILEPLAEGPPIPNRDSYHATESEITPCIDEPRNSNSVEDQMNGRDF 283

Query: 1705 VLKRSYSFGFERSLATTDRIVLEAVTATPWRYRRGNHLWTKRHPSPF--LGKGSRVFSFR 1532
            +LKRSYSFGFERSLA ++R++LE  T +PWRYRRG+  W+KR PSPF  L K +RVFSFR
Sbjct: 284  LLKRSYSFGFERSLA-SERMILEPATTSPWRYRRGS-FWSKR-PSPFGSLPK-ARVFSFR 339

Query: 1531 --RGMKSPFIFRRRHGFFPLSESNARYHG--GGGSSRRTKSMTMASPMFMRST----GGN 1376
              RGMKSPF FRRR GFFPLSES+ R+    GGGSSRR+KSMT  SPMF+RS+    GG 
Sbjct: 340  YYRGMKSPF-FRRRGGFFPLSESSWRFSNGVGGGSSRRSKSMT--SPMFLRSSVTGGGGA 396

Query: 1375 AAFSSSRLRCGDLEALLSPER 1313
            AAFSSSRLRCGD EALLSPER
Sbjct: 397  AAFSSSRLRCGDPEALLSPER 417


>ref|XP_002281140.2| PREDICTED: RING-H2 finger protein ATL65-like [Vitis vinifera]
          Length = 396

 Score =  261 bits (666), Expect = 7e-67
 Identities = 159/251 (63%), Positives = 188/251 (74%), Gaps = 17/251 (6%)
 Frame = -1

Query: 2014 SHDCAVCLVEFEENDYVRTLPICSHAFHVECIDIWLTTHANCPLCRAGIFRLES----LM 1847
            S DCAVCL+EFE+ND VR LP+CSHAFHV+CIDIWL +HANCPLCRAGIFR ES    +M
Sbjct: 152  SRDCAVCLLEFEDNDCVRVLPVCSHAFHVDCIDIWLRSHANCPLCRAGIFRHESPFIPVM 211

Query: 1846 ASRIRPSL-DGILESFLSE------TRSVIQETEISDQVSSQRITETEER--SRNNVLKR 1694
            A+RIRPSL D ILES L E        S    TEI     S R  ++E+R  +R+ +LKR
Sbjct: 212  AARIRPSLDDAILESILLEPLSEAAPESETTVTEIVPAEPSPRRNQSEDRFNARDFMLKR 271

Query: 1693 SYSFGFERSLATTDRIVLEAVTATPWRYRRGNHLWTKRHPSPF--LGKGSRVFSFR--RG 1526
            SYSFGFER+   ++R+V+E  TA+PWRYRRG+  W+KR PSPF  L K SRVFSFR  RG
Sbjct: 272  SYSFGFERNF-PSERLVMEPATASPWRYRRGS-FWSKR-PSPFGSLTK-SRVFSFRYYRG 327

Query: 1525 MKSPFIFRRRHGFFPLSESNARYHGGGGSSRRTKSMTMASPMFMRSTGGNAAFSSSRLRC 1346
            MKSPF FRRR  FFPLSES+AR+  GGGSSRRTKS+T  SP+F+RS+    A +S+RLRC
Sbjct: 328  MKSPF-FRRRGSFFPLSESHARF-SGGGSSRRTKSLT--SPIFIRSSA--TASASTRLRC 381

Query: 1345 GDLEALLSPER 1313
             DLEALLSPER
Sbjct: 382  RDLEALLSPER 392


>gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papaver somniferum]
          Length = 346

 Score =  256 bits (654), Expect = 2e-65
 Identities = 139/339 (41%), Positives = 215/339 (63%), Gaps = 4/339 (1%)
 Frame = +3

Query: 102  QAEVWKHMYGFAKTIMLRSTISLGIPDIIHNN-GPVTLSQLVTHLPL-KSTSIDRFHHFM 275
            QA++WK +YGFA++++L+  + L I + +HNN  P++LS+L + LP+ +  + DR    M
Sbjct: 12   QAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVNEDRLFRIM 71

Query: 276  RYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQTHPEEFSVWSHVIN 455
            RYLVHM+LF I  D  T+  KY L P +K L+ G +KS+   ++     +  + W H+ +
Sbjct: 72   RYLVHMELFKI--DATTQ--KYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWHHLGD 127

Query: 456  VLDGKKPYWESN-DTSMYEKTEGDPEMNEILNDAMTSHSKFMLPALVSGLMKENVLDGVA 632
             L G    +E     S++     +PE N++ N AM   ++ +  AL +   K    DG++
Sbjct: 128  GLTGNCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANEC-KSIFSDGIS 186

Query: 633  SIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIESVIKDPKLDYVAGDMFTSVPNADAI 812
            ++VDVGG +G   KAI  AFPD+KC++ DL HVI    + P +  ++GDMF S+P+ADAI
Sbjct: 187  TLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSIPSADAI 246

Query: 813  LLKSTLHNYEDDDCIKILNKAKEALPSTGGKVILVEIVVDTENLPLFTSARLSMDVDMML 992
             +K  LH++ DD+CI+IL + KEALP  GGKVI+V++V+D ++   +   RL++D+DMML
Sbjct: 247  FMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDSTHPYAKIRLTLDLDMML 305

Query: 993  -MSGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 1106
               GKERTK+EW+ L   A F  H+V  I A++S+I AY
Sbjct: 306  NTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAY 344