BLASTX nr result
ID: Coptis23_contig00014073
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00014073
(2376 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002272711.1| PREDICTED: protein kinase PVPK-1-like [Vitis... 781 0.0
ref|XP_004141198.1| PREDICTED: protein kinase PVPK-1-like [Cucum... 751 0.0
ref|XP_004158354.1| PREDICTED: protein kinase PVPK-1-like [Cucum... 749 0.0
emb|CAA62476.1| stpk1 protein kinase [Solanum tuberosum] 748 0.0
ref|XP_002298543.1| predicted protein [Populus trichocarpa] gi|2... 738 0.0
>ref|XP_002272711.1| PREDICTED: protein kinase PVPK-1-like [Vitis vinifera]
Length = 678
Score = 781 bits (2016), Expect = 0.0
Identities = 424/676 (62%), Positives = 485/676 (71%), Gaps = 25/676 (3%)
Frame = -3
Query: 2095 GGNTSSIGLKSSSRPVR--------SQSAKNLLSEEVIDNGMKNNGYRADSVVNQKHLNN 1940
G N SSI +SRP R + A + V D+GMK + DSV+N+KH +
Sbjct: 22 GYNLSSIS--GTSRPTRLPPPKQSRNDGASSASYATVPDHGMKTVLHAVDSVINEKHSHK 79
Query: 1939 SVNEQLQKKDVGSVKKSCR-------TVRGNSVLENSLDNMASLKQALEE---------D 1808
+ + + +++ + K V N VL+ + N + + + E D
Sbjct: 80 TAQDHMHLEELHTSSKQLYKSNKDQLVVGPNDVLDKMVKNSSQKQVSAVETKSCIKHPVD 139
Query: 1807 VTEDERXXXXXXXXXXXXXXXXGCETGNDCRLDQCLVVEEQPIGGLASDIPTNKYKSPPD 1628
V+E+ C+ G VVE P L + + SP +
Sbjct: 140 VSEN---CNSSGLIGPENFVPGPCKGG---------VVE--PNSQLTAQSAISFCASPQN 185
Query: 1627 SCWNGSQYMEAKDSFGTTGVSESAS-VDKSSGSTKVSNSCDFVESTKTSTCRGSTGXXXX 1451
S ++ +QY EAK SF T VSE AS V+KS S ++SNSCDF ES KTS RGSTG
Sbjct: 186 SLYSATQYTEAKQSFTNTEVSECASSVEKSGESAEISNSCDFAESRKTSIYRGSTGSDVS 245
Query: 1450 XXXXXXXXXXTVNKPHKGNDTRWDAIQAVRSRDGVLGINHFRLLKRLGCGDIGSVYLSEL 1271
T+ KPHK NDTRW+AIQAVRSRDGVLG+NHFRLLK+LGCGDIG+VYLSEL
Sbjct: 246 DESSSSSFSSTLYKPHKANDTRWEAIQAVRSRDGVLGLNHFRLLKKLGCGDIGTVYLSEL 305
Query: 1270 SRTNTYFALKVMDKVALASRKKLPRAQTEKEILQCLDHPFLPTLYSHFETEKFSCLVMEF 1091
S T +YFA+KVMDK ALASRKKL RAQTE+EILQ LDHPFLPTLY+HFETEKFSCLVMEF
Sbjct: 306 SCTRSYFAMKVMDKGALASRKKLLRAQTEREILQSLDHPFLPTLYTHFETEKFSCLVMEF 365
Query: 1090 CPGGDLHALRQRQPGKHFSENAARFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHI 911
CPGGDLHALRQRQPGK+FSE+AARFYVAEVLLA+EYLHMLGIIYRDLKPENVLVR+DGHI
Sbjct: 366 CPGGDLHALRQRQPGKYFSEHAARFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHI 425
Query: 910 MLSDFDLSLRCVVSPTLVKSSNTELGLSKKNSSYCVQPACIEPTCVIQPACMQPTCFGPR 731
MLSDFDLSLRC V PTLVK SN+ L KNS YCVQPACIEPTCVIQP+C+QPTCF PR
Sbjct: 426 MLSDFDLSLRCAVCPTLVKFSNS--SLESKNSGYCVQPACIEPTCVIQPSCIQPTCFAPR 483
Query: 730 FFXXXXXXXXXXXXKNETFTNVSHLPELLAEPTSARSMSFVGTHEYLAPEIIKGEGHGSA 551
F KN+ + VS LPEL+AEPT+ARSMSFVGTHEYLAPEIIKGEGHGSA
Sbjct: 484 FL-TRAKKEKKAKPKNDIYHQVSPLPELIAEPTTARSMSFVGTHEYLAPEIIKGEGHGSA 542
Query: 550 VDWWTFGIFLYELLYGKTPFKGSGNRATLFNVVGQPLRFPETPTVSFAARDLIRGLLVKE 371
VDWWTFGIFLYELL+GKTPFKG+GNRATLFNVVGQPLRFPE+P VSFAARDLIRGLLVKE
Sbjct: 543 VDWWTFGIFLYELLFGKTPFKGAGNRATLFNVVGQPLRFPESPAVSFAARDLIRGLLVKE 602
Query: 370 PQHRLAYRRGATEIKQHPFFQSVNWALIRCTSPPDVPKPQNMDFSAPNETPAASKTENGS 191
PQHRLAYRRGATE+KQHPFFQSVNWALIRCT+PPD+PKP +DFSA ++T A
Sbjct: 603 PQHRLAYRRGATEVKQHPFFQSVNWALIRCTNPPDMPKPFMIDFSARSDTTKAPTAGKVP 662
Query: 190 GVDVKPSGNYLEIDFF 143
GVDVKPSGNYLEIDFF
Sbjct: 663 GVDVKPSGNYLEIDFF 678
>ref|XP_004141198.1| PREDICTED: protein kinase PVPK-1-like [Cucumis sativus]
Length = 820
Score = 751 bits (1940), Expect = 0.0
Identities = 414/674 (61%), Positives = 474/674 (70%), Gaps = 14/674 (2%)
Frame = -3
Query: 2122 MPAVNGSRNGGNTSSIGLKSSSRPVRSQS---AKNLLSEEVIDNGMKN-----NGYRADS 1967
M ++ ++N +TS L SS +P QS A + S + N K NG S
Sbjct: 157 MSSLLKTQNPASTSR-NLVSSRQPSPKQSRNEATSSTSYAMSQNCAKKPLRHPNG---SS 212
Query: 1966 VVNQKHLNNSVNEQLQKKDVGSVKKSCRTV-RGNSVLENSLDNMASLKQALEEDVTEDER 1790
V N+KH N +Q + + V K +G S L N D+ ++ + E
Sbjct: 213 VSNKKH---PTNLNVQNEGLTDVTKHTHVSNKGKSELVNLTDSSNKSTESSFQKKVSSEG 269
Query: 1789 XXXXXXXXXXXXXXXXGCETGNDCRLDQCLVVEE----QPIGGLASDIPTNKYKSPPDSC 1622
C +G+D +Q L + Q I S SP +S
Sbjct: 270 GGAEPLVKHIIDDSKDCCSSGSDESGNQVLNASKGASTQIIDQRKSQPAEISCPSPQNSL 329
Query: 1621 WNGSQYMEAKDSFGTTGVSESAS-VDKSSGSTKVSNSCDFVESTKTSTCRGSTGXXXXXX 1445
++ + Y EAK SF T VSE AS ++KSS S ++NS D +S KTS RGSTG
Sbjct: 330 YSTTLYAEAKQSFSNTEVSECASSIEKSSESVDITNSLDLDKSRKTSVYRGSTGSDISDE 389
Query: 1444 XXXXXXXXTVNKPHKGNDTRWDAIQAVRSRDGVLGINHFRLLKRLGCGDIGSVYLSELSR 1265
+ KPHK NDTRW+A+Q VRS DG+LG+NHFRLL+RLGCGDIGSVYLSEL+
Sbjct: 390 SNSSSLSNAMYKPHKANDTRWEAMQVVRSHDGMLGLNHFRLLRRLGCGDIGSVYLSELTG 449
Query: 1264 TNTYFALKVMDKVALASRKKLPRAQTEKEILQCLDHPFLPTLYSHFETEKFSCLVMEFCP 1085
T TYFA+KVMDK ALASRKKL RAQTE+EILQ LDHPFLPTLY+HFETEKFSCLVMEFCP
Sbjct: 450 TKTYFAMKVMDKAALASRKKLLRAQTEREILQSLDHPFLPTLYTHFETEKFSCLVMEFCP 509
Query: 1084 GGDLHALRQRQPGKHFSENAARFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHIML 905
GGDLHALRQRQPGK F E+AARFYVAEVLLA+EYLHMLGIIYRDLKPENVLVRDDGHIML
Sbjct: 510 GGDLHALRQRQPGKFFPEHAARFYVAEVLLALEYLHMLGIIYRDLKPENVLVRDDGHIML 569
Query: 904 SDFDLSLRCVVSPTLVKSSNTELGLSKKNSSYCVQPACIEPTCVIQPACMQPTCFGPRFF 725
SDFDLSLRC VSPTLVKSSN+ GL K+S YCVQPACIEPTC++QP C+QP CF PRF
Sbjct: 570 SDFDLSLRCAVSPTLVKSSNS--GLEAKSSGYCVQPACIEPTCIMQPDCIQPACFTPRFL 627
Query: 724 XXXXXXXXXXXXKNETFTNVSHLPELLAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVD 545
K+E + VS LPEL+AEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVD
Sbjct: 628 -SRHRKEKKSKPKSEVYHQVSPLPELIAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVD 686
Query: 544 WWTFGIFLYELLYGKTPFKGSGNRATLFNVVGQPLRFPETPTVSFAARDLIRGLLVKEPQ 365
WWTFGIFLYELL+G+TPFKG+GNRATLFNVVGQPLRFPE+P+VSFAARDLIRGLLVKEPQ
Sbjct: 687 WWTFGIFLYELLFGRTPFKGAGNRATLFNVVGQPLRFPESPSVSFAARDLIRGLLVKEPQ 746
Query: 364 HRLAYRRGATEIKQHPFFQSVNWALIRCTSPPDVPKPQNMDFSAPNETPAASKTENGSGV 185
HRLAYRRGATEIKQHPFFQSVNWALIRC +PP+VP+ +DFS+ ETP E GV
Sbjct: 747 HRLAYRRGATEIKQHPFFQSVNWALIRCANPPEVPRVDVIDFSSRMETPHTPAGEKMPGV 806
Query: 184 DVKPSGNYLEIDFF 143
DVKPSGNYLEIDFF
Sbjct: 807 DVKPSGNYLEIDFF 820
>ref|XP_004158354.1| PREDICTED: protein kinase PVPK-1-like [Cucumis sativus]
Length = 820
Score = 749 bits (1935), Expect = 0.0
Identities = 413/674 (61%), Positives = 474/674 (70%), Gaps = 14/674 (2%)
Frame = -3
Query: 2122 MPAVNGSRNGGNTSSIGLKSSSRPVRSQS---AKNLLSEEVIDNGMKN-----NGYRADS 1967
M ++ ++N +TS L SS +P QS A + S + N K NG S
Sbjct: 157 MSSLLKTQNPASTSR-NLVSSRQPSPKQSRNEATSSTSYAMSQNCAKKPLRHPNG---SS 212
Query: 1966 VVNQKHLNNSVNEQLQKKDVGSVKKSCRTV-RGNSVLENSLDNMASLKQALEEDVTEDER 1790
V ++KH N +Q + + V K +G S L N D+ ++ + E
Sbjct: 213 VSSKKH---PTNLNVQNEGLTDVTKHTHVSNKGKSELVNLTDSSNKSTESSFQKKVSSEG 269
Query: 1789 XXXXXXXXXXXXXXXXGCETGNDCRLDQCLVVEE----QPIGGLASDIPTNKYKSPPDSC 1622
C +G+D +Q L + Q I S SP +S
Sbjct: 270 GGAEPLVKHIIDDSKDCCSSGSDESGNQVLNASKGASTQIIDQRKSQPAEISCPSPQNSL 329
Query: 1621 WNGSQYMEAKDSFGTTGVSESAS-VDKSSGSTKVSNSCDFVESTKTSTCRGSTGXXXXXX 1445
++ + Y EAK SF T VSE AS ++KSS S ++NS D +S KTS RGSTG
Sbjct: 330 YSTTLYAEAKQSFSNTEVSECASSIEKSSESVDITNSLDLDKSRKTSVYRGSTGSDISDE 389
Query: 1444 XXXXXXXXTVNKPHKGNDTRWDAIQAVRSRDGVLGINHFRLLKRLGCGDIGSVYLSELSR 1265
+ KPHK NDTRW+A+Q VRS DG+LG+NHFRLL+RLGCGDIGSVYLSEL+
Sbjct: 390 SNSSSLSNAMYKPHKANDTRWEAMQVVRSHDGMLGLNHFRLLRRLGCGDIGSVYLSELTG 449
Query: 1264 TNTYFALKVMDKVALASRKKLPRAQTEKEILQCLDHPFLPTLYSHFETEKFSCLVMEFCP 1085
T TYFA+KVMDK ALASRKKL RAQTE+EILQ LDHPFLPTLY+HFETEKFSCLVMEFCP
Sbjct: 450 TKTYFAMKVMDKAALASRKKLLRAQTEREILQSLDHPFLPTLYTHFETEKFSCLVMEFCP 509
Query: 1084 GGDLHALRQRQPGKHFSENAARFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHIML 905
GGDLHALRQRQPGK F E+AARFYVAEVLLA+EYLHMLGIIYRDLKPENVLVRDDGHIML
Sbjct: 510 GGDLHALRQRQPGKFFPEHAARFYVAEVLLALEYLHMLGIIYRDLKPENVLVRDDGHIML 569
Query: 904 SDFDLSLRCVVSPTLVKSSNTELGLSKKNSSYCVQPACIEPTCVIQPACMQPTCFGPRFF 725
SDFDLSLRC VSPTLVKSSN+ GL K+S YCVQPACIEPTC++QP C+QP CF PRF
Sbjct: 570 SDFDLSLRCAVSPTLVKSSNS--GLEAKSSGYCVQPACIEPTCIMQPDCIQPACFTPRFL 627
Query: 724 XXXXXXXXXXXXKNETFTNVSHLPELLAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVD 545
K+E + VS LPEL+AEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVD
Sbjct: 628 -SRHRKEKKSKPKSEVYHQVSPLPELIAEPTSARSMSFVGTHEYLAPEIIKGEGHGSAVD 686
Query: 544 WWTFGIFLYELLYGKTPFKGSGNRATLFNVVGQPLRFPETPTVSFAARDLIRGLLVKEPQ 365
WWTFGIFLYELL+G+TPFKG+GNRATLFNVVGQPLRFPE+P+VSFAARDLIRGLLVKEPQ
Sbjct: 687 WWTFGIFLYELLFGRTPFKGAGNRATLFNVVGQPLRFPESPSVSFAARDLIRGLLVKEPQ 746
Query: 364 HRLAYRRGATEIKQHPFFQSVNWALIRCTSPPDVPKPQNMDFSAPNETPAASKTENGSGV 185
HRLAYRRGATEIKQHPFFQSVNWALIRC +PP+VP+ +DFS+ ETP E GV
Sbjct: 747 HRLAYRRGATEIKQHPFFQSVNWALIRCANPPEVPRVDVIDFSSRMETPHTPAGEKMPGV 806
Query: 184 DVKPSGNYLEIDFF 143
DVKPSGNYLEIDFF
Sbjct: 807 DVKPSGNYLEIDFF 820
>emb|CAA62476.1| stpk1 protein kinase [Solanum tuberosum]
Length = 631
Score = 748 bits (1932), Expect = 0.0
Identities = 386/515 (74%), Positives = 421/515 (81%), Gaps = 4/515 (0%)
Frame = -3
Query: 1675 GLASDIPTNKYKSPPDSCWNGSQYMEAKDSFGTTGVSESAS--VDKSSGSTKVSNSCDFV 1502
GL S+ SP +S ++ +QY EAK SF T VSE AS VDKS S VSNSCD V
Sbjct: 124 GLPSESGITYCPSPQNSFYSATQYTEAKQSFSNTEVSECASSTVDKSGESGDVSNSCDLV 183
Query: 1501 ESTKTSTCRGSTGXXXXXXXXXXXXXXT-VNKPHKGNDTRWDAIQAVRSRDGVLGINHFR 1325
ES KTS RGSTG T V KPHK NDTRWDAIQ +R+R+G LG NHFR
Sbjct: 184 ESRKTSFYRGSTGSDVSDESSSSSFNSTTVYKPHKANDTRWDAIQVIRAREGTLGFNHFR 243
Query: 1324 LLKRLGCGDIGSVYLSELSRTNTYFALKVMDKVALASRKKLPRAQTEKEILQCLDHPFLP 1145
LLKRLGCGDIGSV+L+EL T +FA+KVMDK AL SRKKL RAQTE+EILQ LDHPFLP
Sbjct: 244 LLKRLGCGDIGSVFLAELIGTRCFFAMKVMDKAALESRKKLVRAQTEREILQSLDHPFLP 303
Query: 1144 TLYSHFETEKFSCLVMEFCPGGDLHALRQRQPGKHFSENAARFYVAEVLLAMEYLHMLGI 965
TLYSHFET+KFSCLVMEFCPGGDLHALRQ+QPGK F E+AARFYVAEVLLA+EYLHMLGI
Sbjct: 304 TLYSHFETDKFSCLVMEFCPGGDLHALRQKQPGKFFPEHAARFYVAEVLLALEYLHMLGI 363
Query: 964 IYRDLKPENVLVRDDGHIMLSDFDLSLRCVVSPTLVKSSNTELGLSKKNSSYCVQPACIE 785
IYRDLKPENVLVR+DGHIMLSDFDLSLRC VSPTLV+SSN+ L K+SSYCVQPACIE
Sbjct: 364 IYRDLKPENVLVREDGHIMLSDFDLSLRCAVSPTLVRSSNS--SLESKSSSYCVQPACIE 421
Query: 784 PTCVIQPACMQPTCFGPRFFXXXXXXXXXXXXKNETFTNVSH-LPELLAEPTSARSMSFV 608
P+CVIQPAC+QP+CF PRF K ET+ V+ LPELLAEPTSARSMSFV
Sbjct: 422 PSCVIQPACIQPSCFTPRFL-SKTKKEKKSKQKTETYNQVNRPLPELLAEPTSARSMSFV 480
Query: 607 GTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLYGKTPFKGSGNRATLFNVVGQPLRFPE 428
GTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELL+G+TPFKG+GNRATLFNVVGQPL+FPE
Sbjct: 481 GTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLFGQTPFKGAGNRATLFNVVGQPLKFPE 540
Query: 427 TPTVSFAARDLIRGLLVKEPQHRLAYRRGATEIKQHPFFQSVNWALIRCTSPPDVPKPQN 248
TP+VSFAARDLIRGLLVKEPQHRLAYRRGATEIKQHPFFQ+VNWALIRCTSPPDVPKP +
Sbjct: 541 TPSVSFAARDLIRGLLVKEPQHRLAYRRGATEIKQHPFFQNVNWALIRCTSPPDVPKPSS 600
Query: 247 MDFSAPNETPAASKTENGSGVDVKPSGNYLEIDFF 143
M ++ P TP A KT GVDVKPSGNY EIDFF
Sbjct: 601 MTYNMP-RTPPAGKT---PGVDVKPSGNYFEIDFF 631
>ref|XP_002298543.1| predicted protein [Populus trichocarpa] gi|222845801|gb|EEE83348.1|
predicted protein [Populus trichocarpa]
Length = 646
Score = 738 bits (1904), Expect = 0.0
Identities = 377/500 (75%), Positives = 407/500 (81%), Gaps = 1/500 (0%)
Frame = -3
Query: 1639 SPPDSCWNGSQYMEAKDSFGTTGVSESAS-VDKSSGSTKVSNSCDFVESTKTSTCRGSTG 1463
SP +S + + Y EAK SF T VSE AS V+KS S +VSNS D ES K S RGST
Sbjct: 150 SPSNSIYTATLYAEAKQSFTNTEVSEEASIVEKSVESGEVSNSYDN-ESRKMSLYRGSTD 208
Query: 1462 XXXXXXXXXXXXXXTVNKPHKGNDTRWDAIQAVRSRDGVLGINHFRLLKRLGCGDIGSVY 1283
+ KPHK ND RW+AIQAVRS G LG NHF+LLKRLGCGDIGSVY
Sbjct: 209 SDISDESSSSSLSSALYKPHKANDIRWEAIQAVRSHAGALGFNHFKLLKRLGCGDIGSVY 268
Query: 1282 LSELSRTNTYFALKVMDKVALASRKKLPRAQTEKEILQCLDHPFLPTLYSHFETEKFSCL 1103
LSELS T T FA+KVMDK ALA+RKKL RAQTE+EILQ LDHPFLPTLYSHFETEKFSCL
Sbjct: 269 LSELSGTRTCFAMKVMDKAALAARKKLLRAQTEREILQSLDHPFLPTLYSHFETEKFSCL 328
Query: 1102 VMEFCPGGDLHALRQRQPGKHFSENAARFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRD 923
+MEFCPGGDLHALRQRQPGK+F E+AARFYVAEVLLA+EYLHMLGIIYRDLKPENVLVR+
Sbjct: 329 LMEFCPGGDLHALRQRQPGKYFPEHAARFYVAEVLLALEYLHMLGIIYRDLKPENVLVRE 388
Query: 922 DGHIMLSDFDLSLRCVVSPTLVKSSNTELGLSKKNSSYCVQPACIEPTCVIQPACMQPTC 743
DGHIMLSDFDLSLRC V PTLVKSSNT KNS+YCVQPACIEPTCVIQP C+QP C
Sbjct: 389 DGHIMLSDFDLSLRCAVCPTLVKSSNTSS--ESKNSAYCVQPACIEPTCVIQPDCIQPAC 446
Query: 742 FGPRFFXXXXXXXXXXXXKNETFTNVSHLPELLAEPTSARSMSFVGTHEYLAPEIIKGEG 563
FGPRFF KNE V+ LPEL+AEPT+ARSMSFVGTHEYLAPEIIKGEG
Sbjct: 447 FGPRFFSTKAKKGKKSKSKNEMNHQVNPLPELMAEPTNARSMSFVGTHEYLAPEIIKGEG 506
Query: 562 HGSAVDWWTFGIFLYELLYGKTPFKGSGNRATLFNVVGQPLRFPETPTVSFAARDLIRGL 383
HGSAVDWWTFGIFLYELL+GKTPFKG+GNRATLFNVVGQPLRFPE+PTVSFAARDLIRGL
Sbjct: 507 HGSAVDWWTFGIFLYELLFGKTPFKGAGNRATLFNVVGQPLRFPESPTVSFAARDLIRGL 566
Query: 382 LVKEPQHRLAYRRGATEIKQHPFFQSVNWALIRCTSPPDVPKPQNMDFSAPNETPAASKT 203
LVKEPQHRLAYRRGATEIKQHPFFQS+NWALIRCT+PP+VPK M+F ++P A
Sbjct: 567 LVKEPQHRLAYRRGATEIKQHPFFQSINWALIRCTNPPEVPKQAIMEFLMRADSPKAPTD 626
Query: 202 ENGSGVDVKPSGNYLEIDFF 143
N GVDVKPSGNYLEIDFF
Sbjct: 627 VNVPGVDVKPSGNYLEIDFF 646