BLASTX nr result
ID: Coptis23_contig00000831
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00000831
(1688 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002325856.1| vitamin-b12 independent methionine synthase,... 553 e-155
sp|Q42699.1|METE_CATRO RecName: Full=5-methyltetrahydropteroyltr... 550 e-154
gb|AEO86798.1| methionine synthase [Camellia sinensis] 548 e-153
gb|ABS01352.1| methionine synthase [Carica papaya] 548 e-153
ref|XP_003561625.1| PREDICTED: 5-methyltetrahydropteroyltrigluta... 546 e-153
>ref|XP_002325856.1| vitamin-b12 independent methionine synthase,
5-methyltetrahydropteroyltriglutamate-homocysteine
[Populus trichocarpa] gi|222862731|gb|EEF00238.1|
vitamin-b12 independent methionine synthase,
5-methyltetrahydropteroyltriglutamate-homocysteine
[Populus trichocarpa]
Length = 765
Score = 553 bits (1424), Expect = e-155
Identities = 281/328 (85%), Positives = 298/328 (90%)
Frame = +2
Query: 2 DTNYHFIVPELGPETKFSYASHKAVTEYKEAKQLGIDTVPVLVGPVSYLLLSKPAKGVEK 181
DTNYHFIVPELGP+ KFSYASHKAVTEYKEAK LG+DTVPVL+GPVSYLLLSKPAKGVEK
Sbjct: 114 DTNYHFIVPELGPDVKFSYASHKAVTEYKEAKALGVDTVPVLIGPVSYLLLSKPAKGVEK 173
Query: 182 SFSLLSLIGKILPIYKEVVAELKAAGASWIQFDEPTLVKDLEPHQLQAFTSTYAELESSL 361
SFSLLSL+GKILPIYKEV++ELK AGASWIQFDEPTLV DLE H+LQAFT Y+ LES+L
Sbjct: 174 SFSLLSLLGKILPIYKEVISELKEAGASWIQFDEPTLVMDLESHKLQAFTGAYSALESTL 233
Query: 362 TGLNVLIETYFADIPAETYKTLTALKGVTGFGFDFIRGSKTLDLIKGGFPSGKYLFAGLV 541
+GLNVLIETYFADIPAE YKTLT+LKGVT FGFD +RG+KTLDLIKG FP GKYLFAG+V
Sbjct: 234 SGLNVLIETYFADIPAEQYKTLTSLKGVTAFGFDLVRGTKTLDLIKGDFPKGKYLFAGVV 293
Query: 542 DGRNIWANDLTASLSTLQALEGIVGKDKLVVSTSTSLLHTAVDLVNETKLDDEIKSWLAF 721
DGRNIWANDL AS STL+ LEGIVGKDKLVVSTS SLLHTAVDLVNETKLD EIKSWLAF
Sbjct: 294 DGRNIWANDLAASFSTLETLEGIVGKDKLVVSTSCSLLHTAVDLVNETKLDKEIKSWLAF 353
Query: 722 AAQKVVEVNALAKALAGQKDEXXXXXXXXXXXSRKSSPRVTNEAVQKASAALQGSDHRRA 901
AAQKVVEVNALAKALAGQKDE SRKSSPRV NEAVQKA+AAL+GSDHRRA
Sbjct: 354 AAQKVVEVNALAKALAGQKDEEIFSANAAALASRKSSPRVNNEAVQKAAAALKGSDHRRA 413
Query: 902 TNVSARLDAQQKKLNLPILPTTTIGSFP 985
TNVSARLDAQQKKLNLPILPTTTIGSFP
Sbjct: 414 TNVSARLDAQQKKLNLPILPTTTIGSFP 441
Score = 376 bits (966), Expect = e-102
Identities = 179/192 (93%), Positives = 187/192 (97%)
Frame = +1
Query: 1111 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 1290
VKLQEE DIDVLVHGEPERNDMVEYFGEQLSGFAF+ NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 475 VKLQEEFDIDVLVHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIYGDVSR 534
Query: 1291 PKGMTVFWSSMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 1470
PK MTVFWS++AQSMT+RPMKGMLTGPVTILNWSFVRNDQPR+ETCYQIALAIKDEVEDL
Sbjct: 535 PKSMTVFWSTLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYETCYQIALAIKDEVEDL 594
Query: 1471 EKAGITVIQIDEAALREGLPLRKSEEAFYLEWAVHSFRITNCGVEDTTQIHTHMCYSNFN 1650
EK GITVIQIDEAALREGLPLRKSE+AFYL+WAVHSFRITNCGV+DTTQIHTHMCYSNFN
Sbjct: 595 EKNGITVIQIDEAALREGLPLRKSEQAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFN 654
Query: 1651 DIIHSIIDMDAD 1686
DII SIIDMDAD
Sbjct: 655 DIIQSIIDMDAD 666
>sp|Q42699.1|METE_CATRO RecName: Full=5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase; AltName: Full=Cobalamin-independent
methionine synthase isozyme; AltName:
Full=Vitamin-B12-independent methionine synthase isozyme
gi|886471|emb|CAA58474.1| methionine synthase
[Catharanthus roseus]
Length = 765
Score = 550 bits (1416), Expect = e-154
Identities = 276/328 (84%), Positives = 300/328 (91%)
Frame = +2
Query: 2 DTNYHFIVPELGPETKFSYASHKAVTEYKEAKQLGIDTVPVLVGPVSYLLLSKPAKGVEK 181
DTNYH+IVPELGPE FSYASHKAV EYKEAK+LG+DTVPVLVGPV++LLLSKPAKGVEK
Sbjct: 114 DTNYHYIVPELGPEVNFSYASHKAVNEYKEAKELGVDTVPVLVGPVTFLLLSKPAKGVEK 173
Query: 182 SFSLLSLIGKILPIYKEVVAELKAAGASWIQFDEPTLVKDLEPHQLQAFTSTYAELESSL 361
+F LLSL+ KILP+YKEV+ ELKAAGASWIQFDEPTLV DLE HQL+AFT Y+ELES+L
Sbjct: 174 TFPLLSLLDKILPVYKEVIGELKAAGASWIQFDEPTLVLDLESHQLEAFTKAYSELESTL 233
Query: 362 TGLNVLIETYFADIPAETYKTLTALKGVTGFGFDFIRGSKTLDLIKGGFPSGKYLFAGLV 541
+GLNV++ETYFADIPAETYK LTALKGVTGFGFD +RG+KTLDLIKGGFPSGKYLFAG+V
Sbjct: 234 SGLNVIVETYFADIPAETYKILTALKGVTGFGFDLVRGAKTLDLIKGGFPSGKYLFAGVV 293
Query: 542 DGRNIWANDLTASLSTLQALEGIVGKDKLVVSTSTSLLHTAVDLVNETKLDDEIKSWLAF 721
DGRNIWANDL ASLSTLQ+LEGIVGKDKLVVSTS SLLHTAVDLVNE KLD EIKSWLAF
Sbjct: 294 DGRNIWANDLAASLSTLQSLEGIVGKDKLVVSTSCSLLHTAVDLVNEPKLDKEIKSWLAF 353
Query: 722 AAQKVVEVNALAKALAGQKDEXXXXXXXXXXXSRKSSPRVTNEAVQKASAALQGSDHRRA 901
AAQKVVEVNALAKALAG+KDE SRKSSPRVTN+AVQKA+AAL+GSDHRRA
Sbjct: 354 AAQKVVEVNALAKALAGEKDEAFFSENAAAQASRKSSPRVTNQAVQKAAAALRGSDHRRA 413
Query: 902 TNVSARLDAQQKKLNLPILPTTTIGSFP 985
T VSARLDAQQKKLNLP+LPTTTIGSFP
Sbjct: 414 TTVSARLDAQQKKLNLPVLPTTTIGSFP 441
Score = 369 bits (948), Expect = 1e-99
Identities = 178/192 (92%), Positives = 182/192 (94%)
Frame = +1
Query: 1111 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 1290
VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 475 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSR 534
Query: 1291 PKGMTVFWSSMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 1470
P MTVFWS AQSMT RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL
Sbjct: 535 PNPMTVFWSQTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 594
Query: 1471 EKAGITVIQIDEAALREGLPLRKSEEAFYLEWAVHSFRITNCGVEDTTQIHTHMCYSNFN 1650
EKAGI VIQIDEAALREGLPLRK+E AFYL+WAVHSFRITN ++DTTQIHTHMCYSNFN
Sbjct: 595 EKAGINVIQIDEAALREGLPLRKAEHAFYLDWAVHSFRITNLPLQDTTQIHTHMCYSNFN 654
Query: 1651 DIIHSIIDMDAD 1686
DIIHSIIDMDAD
Sbjct: 655 DIIHSIIDMDAD 666
>gb|AEO86798.1| methionine synthase [Camellia sinensis]
Length = 765
Score = 548 bits (1413), Expect = e-153
Identities = 279/328 (85%), Positives = 299/328 (91%)
Frame = +2
Query: 2 DTNYHFIVPELGPETKFSYASHKAVTEYKEAKQLGIDTVPVLVGPVSYLLLSKPAKGVEK 181
DTNYHFIVPELGPE KFSYASHKAV EYKEAK LG+DTVPVLVGPVSYLLLSKPAKGVEK
Sbjct: 114 DTNYHFIVPELGPEIKFSYASHKAVDEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEK 173
Query: 182 SFSLLSLIGKILPIYKEVVAELKAAGASWIQFDEPTLVKDLEPHQLQAFTSTYAELESSL 361
SFSLLSL+ KILPIYKEVVAELKAAGASWIQFDEPTLVKDLE HQLQAFT+ Y+ELES+L
Sbjct: 174 SFSLLSLLDKILPIYKEVVAELKAAGASWIQFDEPTLVKDLESHQLQAFTAAYSELESAL 233
Query: 362 TGLNVLIETYFADIPAETYKTLTALKGVTGFGFDFIRGSKTLDLIKGGFPSGKYLFAGLV 541
+GLNV++ETYFAD+PAE +KTLT+LK VTGFGFD +RG++TLDLI GFPSG+YLFAG+V
Sbjct: 234 SGLNVIVETYFADVPAEAFKTLTSLKCVTGFGFDLVRGAQTLDLITSGFPSGEYLFAGVV 293
Query: 542 DGRNIWANDLTASLSTLQALEGIVGKDKLVVSTSTSLLHTAVDLVNETKLDDEIKSWLAF 721
DGRNIWANDL ASLS LQ LEGIVGKDKLVVSTS SLLHTAVDLVNETK D E+KSWLAF
Sbjct: 294 DGRNIWANDLAASLSLLQTLEGIVGKDKLVVSTSCSLLHTAVDLVNETKFDKELKSWLAF 353
Query: 722 AAQKVVEVNALAKALAGQKDEXXXXXXXXXXXSRKSSPRVTNEAVQKASAALQGSDHRRA 901
AAQKVVEVN+LAKALAG KDE SRKSSPRVTNEAVQKA+AAL+GSDHRRA
Sbjct: 354 AAQKVVEVNSLAKALAGHKDEAFFSENAAAQASRKSSPRVTNEAVQKAAAALRGSDHRRA 413
Query: 902 TNVSARLDAQQKKLNLPILPTTTIGSFP 985
TNVSARLDAQQKKLNLPILPTTTIGSFP
Sbjct: 414 TNVSARLDAQQKKLNLPILPTTTIGSFP 441
Score = 381 bits (978), Expect = e-103
Identities = 182/192 (94%), Positives = 188/192 (97%)
Frame = +1
Query: 1111 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 1290
VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAF+ NGWVQSYGSRCVKPPII+GDVSR
Sbjct: 475 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFSANGWVQSYGSRCVKPPIIFGDVSR 534
Query: 1291 PKGMTVFWSSMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 1470
PK MTVFWSSMAQSMT+RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL
Sbjct: 535 PKAMTVFWSSMAQSMTTRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 594
Query: 1471 EKAGITVIQIDEAALREGLPLRKSEEAFYLEWAVHSFRITNCGVEDTTQIHTHMCYSNFN 1650
EKAGI VIQ+DEAALREGLPLRKSE+AFYL+WAVHSFRITNCGV DTTQIHTHMCYSNFN
Sbjct: 595 EKAGINVIQMDEAALREGLPLRKSEQAFYLDWAVHSFRITNCGVTDTTQIHTHMCYSNFN 654
Query: 1651 DIIHSIIDMDAD 1686
DIIHSIIDMDAD
Sbjct: 655 DIIHSIIDMDAD 666
>gb|ABS01352.1| methionine synthase [Carica papaya]
Length = 765
Score = 548 bits (1411), Expect = e-153
Identities = 275/328 (83%), Positives = 300/328 (91%)
Frame = +2
Query: 2 DTNYHFIVPELGPETKFSYASHKAVTEYKEAKQLGIDTVPVLVGPVSYLLLSKPAKGVEK 181
DTNYHFIVPELGP+ FSYASHKAVTEYKEAK LG+DTVPVLVGPVSYLLLSKPAKGVEK
Sbjct: 114 DTNYHFIVPELGPDVTFSYASHKAVTEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEK 173
Query: 182 SFSLLSLIGKILPIYKEVVAELKAAGASWIQFDEPTLVKDLEPHQLQAFTSTYAELESSL 361
+FSLLSL+ KILP+YKEV++ELKAAGASWIQFDEPTLV DL+ H+LQAFT Y+ELES+L
Sbjct: 174 TFSLLSLLNKILPVYKEVISELKAAGASWIQFDEPTLVLDLDAHKLQAFTDAYSELESTL 233
Query: 362 TGLNVLIETYFADIPAETYKTLTALKGVTGFGFDFIRGSKTLDLIKGGFPSGKYLFAGLV 541
+GL+VLIETYFAD+PAE YKTLT LKGVTG+GFD +RG+KTLDLIKGGFP GKYLF+G+V
Sbjct: 234 SGLSVLIETYFADVPAEAYKTLTGLKGVTGYGFDLVRGTKTLDLIKGGFPKGKYLFSGVV 293
Query: 542 DGRNIWANDLTASLSTLQALEGIVGKDKLVVSTSTSLLHTAVDLVNETKLDDEIKSWLAF 721
DGRNIWANDL +SLSTLQ LEGIVGK+ LVVSTS SLLHTAVDLVNETKLD EIKSWLAF
Sbjct: 294 DGRNIWANDLASSLSTLQELEGIVGKENLVVSTSCSLLHTAVDLVNETKLDKEIKSWLAF 353
Query: 722 AAQKVVEVNALAKALAGQKDEXXXXXXXXXXXSRKSSPRVTNEAVQKASAALQGSDHRRA 901
AAQKVVEVNALA+ALAGQKDE SRKSSPRVTNEAVQKA+AAL+GSDHRRA
Sbjct: 354 AAQKVVEVNALAQALAGQKDEAFFSANAAAQASRKSSPRVTNEAVQKAAAALKGSDHRRA 413
Query: 902 TNVSARLDAQQKKLNLPILPTTTIGSFP 985
TNVSARLDAQQKKLNLP+LPTTTIGSFP
Sbjct: 414 TNVSARLDAQQKKLNLPVLPTTTIGSFP 441
Score = 375 bits (963), Expect = e-101
Identities = 182/192 (94%), Positives = 185/192 (96%)
Frame = +1
Query: 1111 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 1290
V LQE+LDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 475 VALQEDLDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 534
Query: 1291 PKGMTVFWSSMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 1470
P MTVFWSS AQSMTSRPMKGMLTGPVTILNWSFVRNDQPR ETCYQIALAIKDEVEDL
Sbjct: 535 PNPMTVFWSSTAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDL 594
Query: 1471 EKAGITVIQIDEAALREGLPLRKSEEAFYLEWAVHSFRITNCGVEDTTQIHTHMCYSNFN 1650
EKAGI VIQIDEAALREGLPLRKSE+AFYLEWAVHSFRITN GV+DTTQIHTHMCYSNFN
Sbjct: 595 EKAGINVIQIDEAALREGLPLRKSEQAFYLEWAVHSFRITNVGVQDTTQIHTHMCYSNFN 654
Query: 1651 DIIHSIIDMDAD 1686
DIIHSIIDMDAD
Sbjct: 655 DIIHSIIDMDAD 666
>ref|XP_003561625.1| PREDICTED: 5-methyltetrahydropteroyltriglutamate--homocysteine
methyltransferase-like [Brachypodium distachyon]
Length = 766
Score = 546 bits (1407), Expect = e-153
Identities = 277/329 (84%), Positives = 299/329 (90%), Gaps = 1/329 (0%)
Frame = +2
Query: 2 DTNYHFIVPELGPETKFSYASHKAVTEYKEAKQLGIDTVPVLVGPVSYLLLSKPAKGVEK 181
DTNYHFIVPELGPETKFSYASHKAV+EYKEAK LG+DTVPVLVGPVSYLLLSKPAKGVEK
Sbjct: 114 DTNYHFIVPELGPETKFSYASHKAVSEYKEAKALGVDTVPVLVGPVSYLLLSKPAKGVEK 173
Query: 182 SFSLLSLIGKILPIYKEVVAELKAAGASWIQFDEPTLVKDLEPHQLQAFTSTYAELESSL 361
SFSLLSL+G ILPIYKEV+AELKAAGASWIQFDEPTLVKDL+ HQL AFTS Y +LESSL
Sbjct: 174 SFSLLSLVGSILPIYKEVIAELKAAGASWIQFDEPTLVKDLDAHQLAAFTSAYTDLESSL 233
Query: 362 TGLNVLIETYFADIPAETYKTLTALKGVTGFGFDFIRGSKTLDLIKG-GFPSGKYLFAGL 538
+GLNVLIETYFAD+PAE+YKTLT+L GVT +GFD +RG+KTLDL+K GFPSGKYLFAG+
Sbjct: 234 SGLNVLIETYFADVPAESYKTLTSLSGVTAYGFDLVRGTKTLDLVKSVGFPSGKYLFAGV 293
Query: 539 VDGRNIWANDLTASLSTLQALEGIVGKDKLVVSTSTSLLHTAVDLVNETKLDDEIKSWLA 718
VDGRNIWA+DL ASLSTLQ+LE +VGKDKLVVSTS SL+HTAVDLVNETKLD EIKSWLA
Sbjct: 294 VDGRNIWADDLAASLSTLQSLEAVVGKDKLVVSTSCSLMHTAVDLVNETKLDSEIKSWLA 353
Query: 719 FAAQKVVEVNALAKALAGQKDEXXXXXXXXXXXSRKSSPRVTNEAVQKASAALQGSDHRR 898
FAAQKVVEVNAL KALAGQKDE SRKSSPRV NE VQKA+AAL+GSDHRR
Sbjct: 354 FAAQKVVEVNALGKALAGQKDEAYFAANAAAQASRKSSPRVNNEEVQKAAAALKGSDHRR 413
Query: 899 ATNVSARLDAQQKKLNLPILPTTTIGSFP 985
AT VSARLDAQQKKLNLP+LPTTTIGSFP
Sbjct: 414 ATTVSARLDAQQKKLNLPVLPTTTIGSFP 442
Score = 370 bits (949), Expect = e-100
Identities = 177/192 (92%), Positives = 182/192 (94%)
Frame = +1
Query: 1111 VKLQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTVNGWVQSYGSRCVKPPIIYGDVSR 1290
VK+QEELDIDVLVHGEPERNDMVEYFGEQLSGFAFT NGWVQSYGSRCVKPPIIYGDVSR
Sbjct: 476 VKIQEELDIDVLVHGEPERNDMVEYFGEQLSGFAFTANGWVQSYGSRCVKPPIIYGDVSR 535
Query: 1291 PKGMTVFWSSMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDL 1470
P MTVFWS +AQSMT RPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIK EVEDL
Sbjct: 536 PNPMTVFWSKIAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKKEVEDL 595
Query: 1471 EKAGITVIQIDEAALREGLPLRKSEEAFYLEWAVHSFRITNCGVEDTTQIHTHMCYSNFN 1650
E GI VIQIDEAALREGLPLRKSE AFYL+WAVHSFRITNCGV+DTTQIHTHMCYSNFN
Sbjct: 596 EAGGIQVIQIDEAALREGLPLRKSEHAFYLDWAVHSFRITNCGVQDTTQIHTHMCYSNFN 655
Query: 1651 DIIHSIIDMDAD 1686
DIIHSII+MDAD
Sbjct: 656 DIIHSIINMDAD 667