BLASTX nr result
ID: Coptis23_contig00000624
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00000624
(2387 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta] 359 0.0
emb|CBI40977.3| unnamed protein product [Vitis vinifera] 228 e-138
ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif... 228 e-138
ref|XP_002282035.1| PREDICTED: cytochrome P450 82A3-like [Vitis ... 223 e-136
ref|XP_002282051.2| PREDICTED: cytochrome P450 82A3-like [Vitis ... 224 e-135
>dbj|BAF98469.1| cytochrome P450 [Coptis japonica var. dissecta]
Length = 511
Score = 359 bits (922), Expect(3) = 0.0
Identities = 177/192 (92%), Positives = 178/192 (92%)
Frame = -2
Query: 2221 FISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI 2042
FISLYFLILWR SSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI
Sbjct: 2 FISLYFLILWRTRSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMADKYGPI 61
Query: 2041 FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE 1862
FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE
Sbjct: 62 FKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYGQYWVE 121
Query: 1861 LRKITMXXXXXXXXXXXLKHVRDSEIDTSIQELYKVWKNHDKAKGPVLVDMKQWFGDLTL 1682
LRKITM LKHVRDSEID SIQ+LYKVWKNHDKAKGPVLVDMKQWFGDLTL
Sbjct: 122 LRKITMSELLSNRRLELLKHVRDSEIDASIQDLYKVWKNHDKAKGPVLVDMKQWFGDLTL 181
Query: 1681 NVILRMIAGKRY 1646
NVILRMIAGKRY
Sbjct: 182 NVILRMIAGKRY 193
Score = 255 bits (652), Expect(3) = 0.0
Identities = 123/125 (98%), Positives = 125/125 (100%)
Frame = -2
Query: 994 VAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGR 815
VAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGR
Sbjct: 387 VAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGR 446
Query: 814 RACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHP 635
R+CPGMSFTLQVVPLVLARFLHEF+SKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHP
Sbjct: 447 RSCPGMSFTLQVVPLVLARFLHEFDSKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHP 506
Query: 634 EIYGL 620
EIYGL
Sbjct: 507 EIYGL 511
Score = 236 bits (603), Expect(3) = 0.0
Identities = 120/139 (86%), Positives = 121/139 (87%)
Frame = -3
Query: 1524 IEDALPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSI 1345
IEDALPYLSW DLQGYKKEMKNTAKELDSVFQRWLEEH RMRQTGELNREQDFMDVLMSI
Sbjct: 224 IEDALPYLSWFDLQGYKKEMKNTAKELDSVFQRWLEEHNRMRQTGELNREQDFMDVLMSI 283
Query: 1344 LEGTKISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVG 1165
LE T+ISEYDNDTIIKSTCLSIVTGG DTTMVTLTWI AQDELDSHVG
Sbjct: 284 LEDTRISEYDNDTIIKSTCLSIVTGGGDTTMVTLTWILSLLLNNKHALKKAQDELDSHVG 343
Query: 1164 KDRHVEESDIKNLVYLQAI 1108
KDR VEESDIKNLVYLQAI
Sbjct: 344 KDRQVEESDIKNLVYLQAI 362
>emb|CBI40977.3| unnamed protein product [Vitis vinifera]
Length = 543
Score = 228 bits (581), Expect(3) = e-138
Identities = 112/197 (56%), Positives = 143/197 (72%)
Frame = -2
Query: 2236 SLLVFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMAD 2057
SLLV IS+ F L + ++S C AP A GAWPIIGHLHL G +L HKTLGAMAD
Sbjct: 43 SLLVVVISICFYRLKIK-AASNAKRCT-APRAGGAWPIIGHLHLFGAQQLTHKTLGAMAD 100
Query: 2056 KYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYG 1877
KYGP+F IR+G+N LV+S+S++A+ECFTT+D+ F++RP+ TASKILGYD+ MFG APYG
Sbjct: 101 KYGPVFTIRLGLNEILVLSSSEMARECFTTHDRVFSTRPSVTASKILGYDFAMFGFAPYG 160
Query: 1876 QYWVELRKITMXXXXXXXXXXXLKHVRDSEIDTSIQELYKVWKNHDKAKGPVLVDMKQWF 1697
YW E+RKI LKH+R SE+ TSI+ELY++W + G V VDMK+WF
Sbjct: 161 SYWREMRKIVTIELLSNHRLDMLKHIRASEVGTSIRELYEMWVSERGTDGRVFVDMKRWF 220
Query: 1696 GDLTLNVILRMIAGKRY 1646
GDLTLN+ +R++ GKRY
Sbjct: 221 GDLTLNLAVRLVGGKRY 237
Score = 159 bits (402), Expect(3) = e-138
Identities = 80/140 (57%), Positives = 99/140 (70%), Gaps = 1/140 (0%)
Frame = -3
Query: 1524 IEDALPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTG-ELNREQDFMDVLMS 1348
+ DA+P+LSWLDL+GYKK MK TAKELDS+F WL+EHK R G E +QDFMDV+++
Sbjct: 254 LSDAIPFLSWLDLKGYKKAMKRTAKELDSLFGGWLQEHKEKRLLGGEGKDDQDFMDVMLT 313
Query: 1347 ILEGTKISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHV 1168
+LE S +D DT+ K+TCL+++ GSDTT VTLTW AQ ELD V
Sbjct: 314 VLEDVNFSGFDADTVNKATCLNLILAGSDTTKVTLTWALSLLLNHPHVLKKAQAELDIQV 373
Query: 1167 GKDRHVEESDIKNLVYLQAI 1108
GKDR V+ESD+KNLVYLQAI
Sbjct: 374 GKDRQVDESDVKNLVYLQAI 393
Score = 156 bits (394), Expect(3) = e-138
Identities = 72/122 (59%), Positives = 92/122 (75%)
Frame = -2
Query: 991 AGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGRR 812
AGY++ AGT+++VN +KI RD VW P EF+PERF+TSH + DV+G H+ELIPFG+GRR
Sbjct: 420 AGYNISAGTQIMVNAWKIHRDERVWCNPKEFQPERFMTSHKDTDVRGQHFELIPFGSGRR 479
Query: 811 ACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHPE 632
+CPG+S LQVV LA LH +E D VDMTE+ GLTN KATPLEV+++PRL E
Sbjct: 480 SCPGISLALQVVHFALASLLHSYEVTKPSDGDVDMTESLGLTNLKATPLEVLLSPRLKAE 539
Query: 631 IY 626
+Y
Sbjct: 540 LY 541
>ref|XP_002268915.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
gi|147794787|emb|CAN66846.1| hypothetical protein
VITISV_002367 [Vitis vinifera]
Length = 528
Score = 228 bits (581), Expect(3) = e-138
Identities = 112/197 (56%), Positives = 143/197 (72%)
Frame = -2
Query: 2236 SLLVFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELRHKTLGAMAD 2057
SLLV IS+ F L + ++S C AP A GAWPIIGHLHL G +L HKTLGAMAD
Sbjct: 14 SLLVVVISICFYRLKIK-AASNAKRCT-APRAGGAWPIIGHLHLFGAQQLTHKTLGAMAD 71
Query: 2056 KYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDYVMFGMAPYG 1877
KYGP+F IR+G+N LV+S+S++A+ECFTT+D+ F++RP+ TASKILGYD+ MFG APYG
Sbjct: 72 KYGPVFTIRLGLNEILVLSSSEMARECFTTHDRVFSTRPSVTASKILGYDFAMFGFAPYG 131
Query: 1876 QYWVELRKITMXXXXXXXXXXXLKHVRDSEIDTSIQELYKVWKNHDKAKGPVLVDMKQWF 1697
YW E+RKI LKH+R SE+ TSI+ELY++W + G V VDMK+WF
Sbjct: 132 SYWREMRKIVTIELLSNHRLDMLKHIRASEVGTSIRELYEMWVSERGTDGRVFVDMKRWF 191
Query: 1696 GDLTLNVILRMIAGKRY 1646
GDLTLN+ +R++ GKRY
Sbjct: 192 GDLTLNLAVRLVGGKRY 208
Score = 159 bits (402), Expect(3) = e-138
Identities = 80/140 (57%), Positives = 99/140 (70%), Gaps = 1/140 (0%)
Frame = -3
Query: 1524 IEDALPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTG-ELNREQDFMDVLMS 1348
+ DA+P+LSWLDL+GYKK MK TAKELDS+F WL+EHK R G E +QDFMDV+++
Sbjct: 239 LSDAIPFLSWLDLKGYKKAMKRTAKELDSLFGGWLQEHKEKRLLGGEGKDDQDFMDVMLT 298
Query: 1347 ILEGTKISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHV 1168
+LE S +D DT+ K+TCL+++ GSDTT VTLTW AQ ELD V
Sbjct: 299 VLEDVNFSGFDADTVNKATCLNLILAGSDTTKVTLTWALSLLLNHPHVLKKAQAELDIQV 358
Query: 1167 GKDRHVEESDIKNLVYLQAI 1108
GKDR V+ESD+KNLVYLQAI
Sbjct: 359 GKDRQVDESDVKNLVYLQAI 378
Score = 156 bits (394), Expect(3) = e-138
Identities = 72/122 (59%), Positives = 92/122 (75%)
Frame = -2
Query: 991 AGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGRR 812
AGY++ AGT+++VN +KI RD VW P EF+PERF+TSH + DV+G H+ELIPFG+GRR
Sbjct: 405 AGYNISAGTQIMVNAWKIHRDERVWCNPKEFQPERFMTSHKDTDVRGQHFELIPFGSGRR 464
Query: 811 ACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRLHPE 632
+CPG+S LQVV LA LH +E D VDMTE+ GLTN KATPLEV+++PRL E
Sbjct: 465 SCPGISLALQVVHFALASLLHSYEVTKPSDGDVDMTESLGLTNLKATPLEVLLSPRLKAE 524
Query: 631 IY 626
+Y
Sbjct: 525 LY 526
>ref|XP_002282035.1| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 528
Score = 223 bits (569), Expect(3) = e-136
Identities = 116/206 (56%), Positives = 142/206 (68%), Gaps = 1/206 (0%)
Frame = -2
Query: 2260 QYLPTPMVSLL-VFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELR 2084
QYL + +L + F+S Y L+ RR S+ + APEA GAWPIIGHLHLLGGS+L
Sbjct: 11 QYLNITTIGVLGILFLSYYLLV--RR---SRAGKRRIAPEAAGAWPIIGHLHLLGGSQLP 65
Query: 2083 HKTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDY 1904
H TLG MADKYGPIF IR+GV+ ALVVS+ ++AKECFTTND A + RP A + LGY+Y
Sbjct: 66 HVTLGTMADKYGPIFTIRLGVHRALVVSSREVAKECFTTNDSAVSGRPKLVAPEHLGYNY 125
Query: 1903 VMFGMAPYGQYWVELRKITMXXXXXXXXXXXLKHVRDSEIDTSIQELYKVWKNHDKAKGP 1724
MF +PY YW E+RKI LK VR SE++TSI+ELYK+W G
Sbjct: 126 AMFAFSPYDAYWREVRKIVNTELLSNRRLELLKDVRASEVETSIKELYKLWAEKKNELGH 185
Query: 1723 VLVDMKQWFGDLTLNVILRMIAGKRY 1646
VLV+MKQWFGDL++NVILRM+ GKRY
Sbjct: 186 VLVEMKQWFGDLSMNVILRMVVGKRY 211
Score = 160 bits (404), Expect(3) = e-136
Identities = 70/118 (59%), Positives = 93/118 (78%)
Frame = -2
Query: 994 VAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGR 815
+ GYHVP GTRL++N K+ RDP VW +P EF+PERFLT+H ++D +G H++L+PFGAGR
Sbjct: 405 IGGYHVPKGTRLVLNLSKLHRDPSVWLDPEEFQPERFLTTHRDVDARGQHFQLLPFGAGR 464
Query: 814 RACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRL 641
R+CPG++F LQ++ L LA FLH FE T + PVDM+E GLTN K+TPLE++I PRL
Sbjct: 465 RSCPGITFALQMLHLALASFLHGFEVSTPSNAPVDMSEIPGLTNIKSTPLEILIAPRL 522
Score = 151 bits (381), Expect(3) = e-136
Identities = 73/139 (52%), Positives = 97/139 (69%)
Frame = -3
Query: 1524 IEDALPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSI 1345
++D +P L WLDL G++K MK TAKE+DS+ Q WLEEH+R + GE N DFMDVL+S+
Sbjct: 242 VKDGIPSLGWLDLGGHEKAMKKTAKEIDSIAQEWLEEHRRRKDWGEDNGMHDFMDVLLSV 301
Query: 1344 LEGTKISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVG 1165
L+G + EYD DTI K+T +++++GG+DT VTLTW A++ELD+HVG
Sbjct: 302 LDGKALPEYDADTINKATSMALISGGTDTMTVTLTWALSLILNNRETLKKAKEELDTHVG 361
Query: 1164 KDRHVEESDIKNLVYLQAI 1108
K+R V SDI LVYLQAI
Sbjct: 362 KERLVNASDISKLVYLQAI 380
>ref|XP_002282051.2| PREDICTED: cytochrome P450 82A3-like [Vitis vinifera]
Length = 525
Score = 224 bits (571), Expect(3) = e-135
Identities = 118/206 (57%), Positives = 144/206 (69%), Gaps = 1/206 (0%)
Frame = -2
Query: 2260 QYLPTPMVSLL-VFFISLYFLILWRRWSSSKTNTCKEAPEAPGAWPIIGHLHLLGGSELR 2084
QYL + +L + F+S Y L+ RR S+ + PEA GAWPIIGHLHLLGGS+L
Sbjct: 11 QYLNVTTIGVLGILFLSYYLLV--RR---SRAGKRRIGPEAAGAWPIIGHLHLLGGSQLP 65
Query: 2083 HKTLGAMADKYGPIFKIRIGVNHALVVSNSDIAKECFTTNDKAFASRPTSTASKILGYDY 1904
H TLG MAD YGP+F IR+GV+ ALVVS+ ++AKEC TTND+A +SRP ASK LGY+Y
Sbjct: 66 HVTLGTMADTYGPVFTIRLGVHRALVVSSWEMAKECLTTNDQAASSRPELLASKHLGYNY 125
Query: 1903 VMFGMAPYGQYWVELRKITMXXXXXXXXXXXLKHVRDSEIDTSIQELYKVWKNHDKAKGP 1724
MFG +PYG YW E+RKI LK VR SE+ TSI+ELY++W+ G
Sbjct: 126 SMFGFSPYGSYWREVRKIISLELLSNRRLELLKDVRASEVVTSIKELYELWEEKKNESGL 185
Query: 1723 VLVDMKQWFGDLTLNVILRMIAGKRY 1646
V V+MKQWFGDLTLNVILRM+AGKRY
Sbjct: 186 VSVEMKQWFGDLTLNVILRMVAGKRY 211
Score = 158 bits (399), Expect(3) = e-135
Identities = 73/139 (52%), Positives = 99/139 (71%)
Frame = -3
Query: 1524 IEDALPYLSWLDLQGYKKEMKNTAKELDSVFQRWLEEHKRMRQTGELNREQDFMDVLMSI 1345
+ DA+P+L WLDL ++K +K TAKE+DS+ Q WLEEH+R + +GE+N QDFMDV++S+
Sbjct: 242 VADAIPFLGWLDLGRHEKTLKKTAKEMDSIAQEWLEEHRRRKDSGEVNSTQDFMDVMLSV 301
Query: 1344 LEGTKISEYDNDTIIKSTCLSIVTGGSDTTMVTLTWIXXXXXXXXXXXXXAQDELDSHVG 1165
L+ + +YD DT+ K+TCL+++ GGSDTT+VTLTW AQ+ELD VG
Sbjct: 302 LDSKNLGDYDADTVNKATCLALIVGGSDTTVVTLTWALSLLLNNRDTLKKAQEELDIQVG 361
Query: 1164 KDRHVEESDIKNLVYLQAI 1108
K+R V E DI LVYLQAI
Sbjct: 362 KERLVNEQDISKLVYLQAI 380
Score = 149 bits (377), Expect(3) = e-135
Identities = 68/118 (57%), Positives = 90/118 (76%)
Frame = -2
Query: 994 VAGYHVPAGTRLIVNTYKIQRDPLVWSEPSEFRPERFLTSHVNMDVKGLHYELIPFGAGR 815
+ GYHV GTRLI+N KIQ+DP +W P+EF+PERFLT+H ++D + H+E IPFGAGR
Sbjct: 405 LGGYHVSKGTRLILNLSKIQKDPRIWMSPTEFQPERFLTTHKDLDPREKHFEFIPFGAGR 464
Query: 814 RACPGMSFTLQVVPLVLARFLHEFESKTEMDMPVDMTETAGLTNAKATPLEVVITPRL 641
RACPG++F LQ++ L LA FL F+ T + VDM E+ GLTN K+TPLEV+I+PR+
Sbjct: 465 RACPGIAFALQMLHLTLANFLQAFDFSTPSNAQVDMCESLGLTNMKSTPLEVLISPRM 522