BLASTX nr result
ID: Coptis23_contig00000508
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis23_contig00000508
(1717 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002511827.1| Glutathione-regulated potassium-efflux syste... 379 e-102
ref|XP_002269352.1| PREDICTED: K(+) efflux antiporter 2, chlorop... 372 e-100
ref|XP_002320782.1| predicted protein [Populus trichocarpa] gi|2... 368 3e-99
ref|XP_004134330.1| PREDICTED: K(+) efflux antiporter 2, chlorop... 358 2e-96
ref|XP_003529954.1| PREDICTED: K(+) efflux antiporter 2, chlorop... 326 1e-86
>ref|XP_002511827.1| Glutathione-regulated potassium-efflux system protein kefB, putative
[Ricinus communis] gi|223549007|gb|EEF50496.1|
Glutathione-regulated potassium-efflux system protein
kefB, putative [Ricinus communis]
Length = 1228
Score = 379 bits (972), Expect = e-102
Identities = 261/593 (44%), Positives = 333/593 (56%), Gaps = 59/593 (9%)
Frame = +2
Query: 116 MDISCCLQQPNLFNYGRSNYLNSQLRFSGRILGYSFRKESKSTSLVWRALSNRK------ 277
MD++C +QQPN F+ R SFR S+V + S++K
Sbjct: 1 MDLACSIQQPNAFHGSEVTCYRVPGRLYSSSRYRSFRYNVVDPSIVLKDRSSKKRSKILA 60
Query: 278 ---------LGFSR------------------------------RLCCQGNDSLAYIDEG 340
L F R +L CQGNDSLAY++ G
Sbjct: 61 YNGSCLNSSLVFGRGFQSHLSCAHSNISSFYCSLGGGFNVLKGAKLHCQGNDSLAYVN-G 119
Query: 341 NGRNSQFRENLDEGA---VSDG----RSGSNSDNESSGLVEVDSSSLENLKGLLSSALKE 499
N RN +F E E + DG R G N + V ++SSL+ LK LL AL+E
Sbjct: 120 NDRNVEFVEGSAESSRVGSEDGVELIRLGENEGEQKE--VVAEASSLDELKELLQKALRE 177
Query: 500 LEVAHINSTMFEDKAQKISEAAIAMQDAATNAWVDVNFVLGNIQDVINEEGVAKEAVQKA 679
LE+A +NSTMFE+KAQ+ISE AIA++D A NAW +VN L IQ V+NEE VAKEA+Q A
Sbjct: 178 LEIARLNSTMFEEKAQRISETAIALKDEAANAWDNVNSTLDTIQGVVNEEAVAKEAIQNA 237
Query: 680 TMAFSMAEARLQFAVESLEAAK-ET----GISVDDSVEGDTENGKINGAXXXXXXXXXXX 844
TMA S+AEARL+ AVES+++AK ET G V D V+ + +
Sbjct: 238 TMALSLAEARLRVAVESIDSAKGETDSPHGSGVSDVVKDIRKEDEA-----------LSD 286
Query: 845 AQDEIVNCRNNLEKCQSELSRLQSKKEEMQKEVDRLSEVAERAQMNALKAEEDVANIMHL 1024
AQDEI+ C+ NL C++EL RLQSKKEE+QKEVDRL+EVAE+AQM+ALKAEEDVAN+M L
Sbjct: 287 AQDEIIECQMNLGNCEAELRRLQSKKEELQKEVDRLNEVAEKAQMDALKAEEDVANVMLL 346
Query: 1025 AEQAVAFEVEATKRVNDAEIALERAEKLLSTSNHDKSDALKSSQVPSTQGYIVKDESLFE 1204
AEQAVAFE+EAT+RVNDAEIAL+RAEKLLS+S+ DK +TQGY+ DE++ E
Sbjct: 347 AEQAVAFELEATQRVNDAEIALQRAEKLLSSSSVDKE---------TTQGYVSGDEAVRE 397
Query: 1205 EEGSQEIPGTVSNEVEGEIATGIDSVPIELSLESQSDVSYLGVGEPNLLDDSLVRENGKL 1384
EE E T +E E + + D + E S++ D + E DDS ENGKL
Sbjct: 398 EEKWSE-GRTADDEKERDASIDADLLVGEPSIDGLLDKASQSSKELYHSDDSSDCENGKL 456
Query: 1385 SIVSFKEAEAEVDKSKNTFQTKKQETQKDRTKDS--SPLSVPKALLNKXXXXXXXXXXXX 1558
++ S KE E E +KSK+ Q KKQE QKD T++S SP + PKALL K
Sbjct: 457 NLDSLKEVEVEAEKSKSGVQPKKQEMQKDITRESSASPTNSPKALLKKSSRFFSASFFSF 516
Query: 1559 XGDGTEFTVASVLHSLITTARMQLPKXXXXXXXXXXXXXXXMNRVERSSQLLQ 1717
DGTE T ASV LI +A+ Q+PK NR ERS+Q+LQ
Sbjct: 517 TVDGTELTPASVFQGLIQSAKQQMPKLILGLVLFGAGVAFYSNRAERSTQMLQ 569
>ref|XP_002269352.1| PREDICTED: K(+) efflux antiporter 2, chloroplastic-like [Vitis
vinifera]
Length = 1207
Score = 372 bits (956), Expect = e-100
Identities = 258/577 (44%), Positives = 327/577 (56%), Gaps = 44/577 (7%)
Frame = +2
Query: 116 MDISCCLQQPNLFNYGRSNYLNSQLRFSGRILGYS--FRKES-KSTSLVWRALSNRKL-- 280
MD +C +QPN+F G + RF R S F S + L+ RA N+K+
Sbjct: 1 MDFACSFRQPNVFLNGEGTSYKTLDRFYSRFRFRSPGFNNNSIGNPKLISRAYPNKKMKK 60
Query: 281 -----GFSR----------------------------RLCCQGNDSLAYIDEGNGRNSQF 361
GF+ R CQ NDSLAYID GNGRN +F
Sbjct: 61 MIAFSGFNMTRVFKQEFEGKNLRRSLIYDFNIALSCSRAKCQSNDSLAYID-GNGRNVEF 119
Query: 362 RENLDEGAVSDGRSGSNSDNESSGLVE---VDSSSLENLKGLLSSALKELEVAHINSTMF 532
E+ DE +++ G + G E V++ SL+ L+ +L A+KELEVA +NSTMF
Sbjct: 120 LESHDESSIAGPDDGDQLNRLGEGEGEGEVVEALSLDELREVLQKAIKELEVASLNSTMF 179
Query: 533 EDKAQKISEAAIAMQDAATNAWVDVNFVLGNIQDVINEEGVAKEAVQKATMAFSMAEARL 712
EDKAQKISEAAIA+QD A AW DVN VL IQ+++NEE +AKEAVQKATMA S+AEARL
Sbjct: 180 EDKAQKISEAAIALQDEAAIAWNDVNSVLNTIQEIVNEECIAKEAVQKATMALSLAEARL 239
Query: 713 QFAVESLEAAKETGISVDDSVEGDTEN-GKING-AXXXXXXXXXXXAQDEIVNCRNNLEK 886
Q A ESLEAAK IS + S E D+E+ ++ G + AQ++I +C+ L
Sbjct: 240 QVAKESLEAAKIVSISPESSRESDSEDESRMEGFSSLRKEEEAFLVAQEDIRHCKATLLS 299
Query: 887 CQSELSRLQSKKEEMQKEVDRLSEVAERAQMNALKAEEDVANIMHLAEQAVAFEVEATKR 1066
C++EL RLQ +KEE+QKEVD+L+E AE+ QM+ALKAEE+VANIM LAEQAVAFE+EAT+
Sbjct: 300 CEAELKRLQCRKEELQKEVDKLNEKAEKTQMDALKAEEEVANIMLLAEQAVAFELEATQH 359
Query: 1067 VNDAEIALERAEKLLSTSNHDKSDALKSSQVPSTQGYIVKDESLFEEE-GSQEIPGTVSN 1243
VNDAEIA+++ EK LS S + + +TQG + DE+L EEE SQ I G VS
Sbjct: 360 VNDAEIAIQKVEKSLSNSQVETPE--------TTQGPVFSDETLVEEEKASQGISGDVSV 411
Query: 1244 EVEGEIATGIDSVPIELSLESQSDVSYLGVGEPNLLDDSLVRENGKLSIVSFKEAEAEVD 1423
E E ++ T S E +SQ E DD +ENGKLS+ S KE EAE +
Sbjct: 412 ERERDMPTEGVSFLSESLSDSQP------FEELKQYDDLSDQENGKLSLESPKEPEAETE 465
Query: 1424 KSKNTFQTKKQETQKDRTKDSSPLSVPKALLNKXXXXXXXXXXXXXGDGTEFTVASVLHS 1603
KSK QTKKQETQKD T+DSS L+ PK LL K DGT+
Sbjct: 466 KSKTGVQTKKQETQKDLTRDSSMLNAPKILLKKSSRFFSASFFSFTVDGTD--------- 516
Query: 1604 LITTARMQLPKXXXXXXXXXXXXXXXMNRVERSSQLL 1714
L+ +AR Q PK NR ERSS +L
Sbjct: 517 LMESARRQFPKLVVGMLLLGAGVTFYSNRAERSSLVL 553
>ref|XP_002320782.1| predicted protein [Populus trichocarpa] gi|222861555|gb|EEE99097.1|
predicted protein [Populus trichocarpa]
Length = 552
Score = 368 bits (944), Expect = 3e-99
Identities = 244/505 (48%), Positives = 311/505 (61%), Gaps = 12/505 (2%)
Frame = +2
Query: 158 YGRSNYLNSQLRFSGRILGYSFRKESKSTSLV--WRALSNRKLGFSRRLCCQGNDSLAYI 331
YG ++S LR G + E + + ++ L + KLG CQGNDSLAYI
Sbjct: 62 YGGGRGIHSHLRVGG------YSSEPLFCNFIDGFKGLRSVKLG------CQGNDSLAYI 109
Query: 332 DEGNGRNSQFRENLDEGAVSDGRSG-----SNSDNESSGLVEVDSSSLENLKGLLSSALK 496
D GNGRN + E DE + SG + E V V++ +L+ LK LL A +
Sbjct: 110 D-GNGRNVENGEGNDESLRAGSNSGFGEGDGRGEKEVETGVVVEALNLDELKELLHKATR 168
Query: 497 ELEVAHINSTMFEDKAQKISEAAIAMQDAATNAWVDVNFVLGNIQDVINEEGVAKEAVQK 676
+LEVA +NSTMFE+KAQ ISE AIA+QD A +AW DVN L IQD++NEEGVAKEA QK
Sbjct: 169 DLEVAQLNSTMFEEKAQSISETAIALQDEAESAWNDVNSTLDLIQDIVNEEGVAKEAAQK 228
Query: 677 ATMAFSMAEARLQFAVESLEAAKETGISVDDSVEGDTEN-GKINGAXXXXXXXXXXXAQD 853
ATMA S+AEARL+ AVES++A KE S++ S E D EN GK AQ+
Sbjct: 229 ATMALSLAEARLKVAVESIKAMKERVDSLEGSGESDAENDGK-------EDYETILAAQN 281
Query: 854 EIVNCRNNLEKCQSELSRLQSKKEEMQKEVDRLSEVAERAQMNALKAEEDVANIMHLAEQ 1033
+I +C+ NL C++EL RLQSKKE +Q EV L+E AE+AQMNALKAEEDVANIM LAEQ
Sbjct: 282 DIRDCQANLANCEAELRRLQSKKEALQNEVSVLNEKAEKAQMNALKAEEDVANIMLLAEQ 341
Query: 1034 AVAFEVEATKRVNDAEIALERAEKLLSTSNHDKSDALKSSQVPSTQGYIVKDESLFEEEG 1213
AVAFE+EAT+RVNDAEIAL++AEK L++S D + + +GY+ DE++ EE
Sbjct: 342 AVAFELEATQRVNDAEIALKKAEKSLASSRVDIQE--------TARGYVSGDEAVIEE-- 391
Query: 1214 SQEIPGTVSNEVEGE--IATGIDSVPIELSLESQSDVSYLGVGEPNLLDDSLVRENGKLS 1387
Q++ G +++VE E + D + E S++ SD + E L DDS ENGKLS
Sbjct: 392 -QKMGGGSASDVEKERDMTVNGDVLVGEPSIDRLSDKTSQSSEELYLSDDSSDHENGKLS 450
Query: 1388 IVSFKEAEAEVDKSKNTFQTKKQETQKDRT--KDSSPLSVPKALLNKXXXXXXXXXXXXX 1561
+ S K+ EAE +KSK+ QTKKQE QKD T SSPLS PKALL K
Sbjct: 451 LDSNKDTEAEAEKSKSGDQTKKQEIQKDLTWESSSSPLSAPKALLMKSSRFFSASFFSFS 510
Query: 1562 GDGTEFTVASVLHSLITTARMQLPK 1636
GD TE T ASV L+ +AR QLP+
Sbjct: 511 GDETEVTAASVFQGLMESARKQLPQ 535
>ref|XP_004134330.1| PREDICTED: K(+) efflux antiporter 2, chloroplastic-like [Cucumis
sativus] gi|449480375|ref|XP_004155876.1| PREDICTED: K(+)
efflux antiporter 2, chloroplastic-like [Cucumis sativus]
Length = 1212
Score = 358 bits (919), Expect = 2e-96
Identities = 231/495 (46%), Positives = 301/495 (60%), Gaps = 11/495 (2%)
Frame = +2
Query: 263 LSNRKLGFSRRLCCQGNDSLAYIDEGNGRNSQFRENLDEGAVSD-----GRSGSNSDNES 427
+S R +G L CQ NDSLA+ID GNGRN ++ + DEG+ S G +GS
Sbjct: 95 ISGRGMGMVH-LECQNNDSLAFID-GNGRNIEYVNSGDEGSSSGPTDGVGSAGSREVGGE 152
Query: 428 SGLVEVDSSSLENLKGLLSSALKELEVAHINSTMFEDKAQKISEAAIAMQDAATNAWVDV 607
+ VE + +++ L+ LL A+KELEVA +NSTMFE++AQKISEAAIA+QD AT AW DV
Sbjct: 153 AETVETNIPTVDELRELLQKAMKELEVARLNSTMFEERAQKISEAAIALQDEATIAWNDV 212
Query: 608 NFVLGNIQDVINEEGVAKEAVQKATMAFSMAEARLQFAVESLEAAK------ETGISVDD 769
N L ++Q ++NEE AKEAVQKATMA S+AEARLQ A+ESLE A+ ET + +D
Sbjct: 213 NSTLDSVQLIVNEEYAAKEAVQKATMALSLAEARLQVAIESLELARRGSDFPETSMDIDG 272
Query: 770 SVEGDTENGKINGAXXXXXXXXXXXAQDEIVNCRNNLEKCQSELSRLQSKKEEMQKEVDR 949
+ + ++ AQ++I CR NLE C +EL RLQSKKEE+QKEVD+
Sbjct: 273 NEDQES----------------LLVAQEDITECRANLEICNAELKRLQSKKEELQKEVDK 316
Query: 950 LSEVAERAQMNALKAEEDVANIMHLAEQAVAFEVEATKRVNDAEIALERAEKLLSTSNHD 1129
L+E+AE+AQ+NALKAEEDVANIM LAEQAVAFE+EA +RVNDAE AL++ EK LS+S D
Sbjct: 317 LNELAEKAQLNALKAEEDVANIMLLAEQAVAFELEAAQRVNDAERALQKMEKSLSSSFVD 376
Query: 1130 KSDALKSSQVPSTQGYIVKDESLFEEEGSQEIPGTVSNEVEGEIATGIDSVPIELSLESQ 1309
D + S V +++ + + E G +S E++ E+ DS+ I+ S
Sbjct: 377 TPDTTQGSNV-------IEEVENEDNKAVLEFSGDISVEMDRELPLNGDSLSIKSLPGSL 429
Query: 1310 SDVSYLGVGEPNLLDDSLVRENGKLSIVSFKEAEAEVDKSKNTFQTKKQETQKDRTKDSS 1489
SD G +P L DS E GKLS S KE E+ +KS QTKKQETQKD T++ S
Sbjct: 430 SDSE--GSDQPYYLSDS---EIGKLSSDSAKEVESGAEKS-IVSQTKKQETQKDLTREGS 483
Query: 1490 PLSVPKALLNKXXXXXXXXXXXXXGDGTEFTVASVLHSLITTARMQLPKXXXXXXXXXXX 1669
PL+ PKALL K DGTEFT A V L+ + + QLPK
Sbjct: 484 PLNSPKALLKKSSRFFSASFFSFTVDGTEFTPALVFQGLLDSTKKQLPKLIVGAVLLGAG 543
Query: 1670 XXXXMNRVERSSQLL 1714
NR +RSSQ++
Sbjct: 544 IAIFANRPDRSSQMI 558
>ref|XP_003529954.1| PREDICTED: K(+) efflux antiporter 2, chloroplastic-like [Glycine max]
Length = 1206
Score = 326 bits (836), Expect = 1e-86
Identities = 214/485 (44%), Positives = 290/485 (59%), Gaps = 13/485 (2%)
Frame = +2
Query: 302 CQGNDSLAYIDEGNGRNSQFRENLDEGAVSDGR---------SGSNSDNESSGLVEVDSS 454
CQGNDSL+Y++ GNGRN E DE + S G E G VE++
Sbjct: 91 CQGNDSLSYVN-GNGRNVGRVEGADEDSDSSAELSEPLGEEEKGQGGRKEDGGGVEIEVQ 149
Query: 455 SLENLKGLLSSALKELEVAHINSTMFEDKAQKISEAAIAMQDAATNAWVDVNFVLGNIQD 634
+++ LK LL A+K LE A +NS +FE+K +KISE AI +QD A +AW +V L IQD
Sbjct: 150 NVDELKELLQKAMKALEAARVNSIIFEEKVKKISETAIFLQDEAASAWNNVTSTLDVIQD 209
Query: 635 VINEEGVAKEAVQKATMAFSMAEARLQFAVESLEAAKETGISVDDSVEGDTENGKING-A 811
++++E VAKEAVQKATMA S+AEARLQ A++SLE KE V D+ +G + K NG
Sbjct: 210 IVSQEFVAKEAVQKATMALSLAEARLQVAIDSLEVTKE----VYDTPQG---SNKSNGDK 262
Query: 812 XXXXXXXXXXXAQDEIVNCRNNLEKCQSELSRLQSKKEEMQKEVDRLSEVAERAQMNALK 991
AQ++I C+ +L C++EL LQ +KEE+Q EV++L E+AE+AQ+ A K
Sbjct: 263 DIIQEEKELLVAQEDIRECQTDLANCENELRCLQCRKEELQNEVNKLHEIAEQAQLKAAK 322
Query: 992 AEEDVANIMHLAEQAVAFEVEATKRVNDAEIALERAEKLLSTSNHDKSDALKSSQVPSTQ 1171
AEEDVANIM LAEQAVA E+EA + +NDAEIAL++A+K S+SN D +D L+ V
Sbjct: 323 AEEDVANIMLLAEQAVAAELEAAQCMNDAEIALQKADKSSSSSNADTADTLQVQDV---- 378
Query: 1172 GYIVKDESLFEEEGSQEIPGTVSNEVEGEIATGIDSVPIELSL---ESQSDVSYLGVGEP 1342
++ EEE Q + G ++ + EI ID P+ L++ E+QS+ + + +
Sbjct: 379 ------VAIPEEEVVQGLSGDDAD--KREIDYLIDGEPL-LAMQLPETQSNNTSKSLEDM 429
Query: 1343 NLLDDSLVRENGKLSIVSFKEAEAEVDKSKNTFQTKKQETQKDRTKDSSPLSVPKALLNK 1522
D ENG+LS+ S KEAE E++KSKN QTKKQETQKD +D+SPL+ PKA L K
Sbjct: 430 VQSDYLRDHENGQLSLDSPKEAEVEIEKSKNVVQTKKQETQKDSARDNSPLA-PKASLKK 488
Query: 1523 XXXXXXXXXXXXXGDGTEFTVASVLHSLITTARMQLPKXXXXXXXXXXXXXXXMNRVERS 1702
D T++T ASV H L+ +A+ QLPK NR ERS
Sbjct: 489 SSRFFPASFFSFTADETDYTPASVFHGLVESAQKQLPKLIVGLLLIGAGLVFYTNRTERS 548
Query: 1703 SQLLQ 1717
+QLLQ
Sbjct: 549 AQLLQ 553