BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00000052
         (461 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   279   2e-73
ref|XP_003633039.1| PREDICTED: isoflavone-7-O-methyltransferase ...   114   6e-24
emb|CAN77288.1| hypothetical protein VITISV_004161 [Vitis vinifera]   114   6e-24
emb|CAN75971.1| hypothetical protein VITISV_009882 [Vitis vinifera]   114   6e-24
gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   114   8e-24

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT;
           AltName: Full=Tetrahydrocolumbamine
           2-O-methyltransferase gi|24210466|dbj|BAC22084.1|
           columbamine O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  279 bits (713), Expect = 2e-73
 Identities = 139/153 (90%), Positives = 144/153 (94%)
 Frame = -1

Query: 461 SLAPYVMLQTHPEGFSIWSHVIDVLDGKKPYWESTYGISMYQKTEGDPEINEILNDAMTS 282
           SLAPYVMLQTHPE FS+WSHVI+VLDGKKPYWES    SMY+KTEGDPEINEILNDAMTS
Sbjct: 111 SLAPYVMLQTHPEEFSVWSHVINVLDGKKPYWESN-DTSMYEKTEGDPEINEILNDAMTS 169

Query: 281 HSKFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIESV 102
           HS FMLPALVSGLMKENVLDGVASIVDVGGNSGVVAK IVDAFP VKCSVMDLNHVIE V
Sbjct: 170 HSTFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERV 229

Query: 101 IKDPKLDYVAGDMFTSVPNADAILLKSTLHNYE 3
           IK+PKLDYVAGDMFTS+PNADAILLKSTLHNYE
Sbjct: 230 IKNPKLDYVAGDMFTSIPNADAILLKSTLHNYE 262


>ref|XP_003633039.1| PREDICTED: isoflavone-7-O-methyltransferase 9 [Vitis vinifera]
          Length = 358

 Score =  114 bits (286), Expect = 6e-24
 Identities = 60/153 (39%), Positives = 98/153 (64%), Gaps = 1/153 (0%)
 Frame = -1

Query: 461 SLAPYVMLQTHPEGFSIWSHVIDVLDGKK-PYWESTYGISMYQKTEGDPEINEILNDAMT 285
           +L+P+V+   HP   + W    D L G     +E+ +G+S +     +PEI  + N+ M 
Sbjct: 114 NLSPFVLTVLHPALVTPWHFFGDWLRGDDLTAFETAHGVSFWDHGSHNPEIFNLFNEGMA 173

Query: 284 SHSKFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIES 105
           S S+ M  ++V+    + V +G++S+VD+GG +G++A+ I +AFP +KC+V DL HV+ +
Sbjct: 174 SDSQMM--SVVNFRELKPVFEGLSSLVDLGGGTGLLARIISEAFPQLKCTVFDLPHVVAN 231

Query: 104 VIKDPKLDYVAGDMFTSVPNADAILLKSTLHNY 6
           + +   L+YV GDMF SVP+ADAILLK  LH++
Sbjct: 232 LPESRNLEYVGGDMFQSVPSADAILLKCVLHDW 264


>emb|CAN77288.1| hypothetical protein VITISV_004161 [Vitis vinifera]
          Length = 297

 Score =  114 bits (286), Expect = 6e-24
 Identities = 60/153 (39%), Positives = 98/153 (64%), Gaps = 1/153 (0%)
 Frame = -1

Query: 461 SLAPYVMLQTHPEGFSIWSHVIDVLDGKK-PYWESTYGISMYQKTEGDPEINEILNDAMT 285
           +L+P+V+   HP   + W    D L G     +E+ +G+S +     +PEI  + N+ M 
Sbjct: 114 NLSPFVLTVLHPXLVTPWHFFGDWLRGDDLTAFETAHGVSFWDHGSHNPEIFNLFNEGMA 173

Query: 284 SHSKFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIES 105
           S S+ M  ++V+    + V +G++S+VD+GG +G++A+ I +AFP +KC+V DL HV+ +
Sbjct: 174 SDSQMM--SVVNFRELKPVFEGLSSLVDLGGGTGLLARIISEAFPQLKCTVFDLPHVVAN 231

Query: 104 VIKDPKLDYVAGDMFTSVPNADAILLKSTLHNY 6
           + +   L+YV GDMF SVP+ADAILLK  LH++
Sbjct: 232 LPESRNLEYVGGDMFQSVPSADAILLKCVLHDW 264


>emb|CAN75971.1| hypothetical protein VITISV_009882 [Vitis vinifera]
          Length = 329

 Score =  114 bits (286), Expect = 6e-24
 Identities = 60/153 (39%), Positives = 98/153 (64%), Gaps = 1/153 (0%)
 Frame = -1

Query: 461 SLAPYVMLQTHPEGFSIWSHVIDVLDGKK-PYWESTYGISMYQKTEGDPEINEILNDAMT 285
           +L+P+V+   HP   + W    D L G     +E+ +G+S +     +PEI  + N+ M 
Sbjct: 85  NLSPFVLTVLHPALVTPWHFFGDWLRGDDLTAFETAHGVSFWDHGSHNPEIFNLFNEGMA 144

Query: 284 SHSKFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIES 105
           S S+ M  ++V+    + V +G++S+VD+GG +G++A+ I +AFP +KC+V DL HV+ +
Sbjct: 145 SDSQMM--SVVNFRELKPVFEGLSSLVDLGGGTGLLARIISEAFPQLKCTVFDLPHVVAN 202

Query: 104 VIKDPKLDYVAGDMFTSVPNADAILLKSTLHNY 6
           + +   L+YV GDMF SVP+ADAILLK  LH++
Sbjct: 203 LPESRNLEYVGGDMFQSVPSADAILLKCVLHDW 235


>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  114 bits (285), Expect = 8e-24
 Identities = 54/152 (35%), Positives = 91/152 (59%)
 Frame = -1

Query: 461 SLAPYVMLQTHPEGFSIWSHVIDVLDGKKPYWESTYGISMYQKTEGDPEINEILNDAMTS 282
           S+ P ++  T  +  + W  + D L      +E   G+++++  EG P+ +++ N+ M  
Sbjct: 110 SMVPMILGMTQKDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAG 169

Query: 281 HSKFMLPALVSGLMKENVLDGVASIVDVGGNSGVVAKAIVDAFPDVKCSVMDLNHVIESV 102
            ++ +  +L+SG    ++  G+ S+VDVGG +G   KAI DAFP +KC++ DL HVI + 
Sbjct: 170 ETRLLTSSLISG--SRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANS 227

Query: 101 IKDPKLDYVAGDMFTSVPNADAILLKSTLHNY 6
              P ++ + GDMF SVP+A AILLK  LH++
Sbjct: 228 YDHPNIERIGGDMFKSVPSAQAILLKLILHDW 259