BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00000051
         (1218 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransf...   674   0.0  
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        275   1e-71
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   260   4e-67
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   259   1e-66
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   254   4e-65

>sp|Q8H9A8.1|COOMT_COPJA RecName: Full=Columbamine O-methyltransferase; Short=CoOMT; AltName:
            Full=Tetrahydrocolumbamine 2-O-methyltransferase
            gi|24210466|dbj|BAC22084.1| columbamine
            O-methyltransferase [Coptis japonica]
          Length = 351

 Score =  674 bits (1740), Expect = 0.0
 Identities = 334/351 (95%), Positives = 342/351 (97%)
 Frame = -3

Query: 1150 MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 971
            MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP
Sbjct: 1    MDTPNTFQNDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGPVTLSQLVTHLP 60

Query: 970  LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLGPYVMLQT 791
            LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSL PYVMLQT
Sbjct: 61   LKSTSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLAPYVMLQT 120

Query: 790  HPEEFSVWSHVINVLDGKKPYWESNDTSMYAKTEGDPEMNELLNDAMTSHSTFMLPALVS 611
            HPEEFSVWSHVINVLDGKKPYWESNDTSMY KTEGDPE+NE+LNDAMTSHSTFMLPALVS
Sbjct: 121  HPEEFSVWSHVINVLDGKKPYWESNDTSMYEKTEGDPEINEILNDAMTSHSTFMLPALVS 180

Query: 610  GLMKENVLDGVASIVDVGGNSGIVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 431
            GLMKENVLDGVASIVDVGGNSG+VAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG
Sbjct: 181  GLMKENVLDGVASIVDVGGNSGVVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 240

Query: 430  DMFTSVPNADAILLKSTLHNYEDDDCIKILRKAKEAIPSSGGKVILVEIVVDIENLPQFT 251
            DMFTS+PNADAILLKSTLHNYEDDDCIKIL  AKEA+PS+GGKVILVEIVVD ENLP FT
Sbjct: 241  DMFTSIPNADAILLKSTLHNYEDDDCIKILNIAKEALPSTGGKVILVEIVVDTENLPLFT 300

Query: 250  SARLSMEVDMMLMGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAYS 98
            SARLSM +DMMLM GKERTKKEWEDLLRKA+FT HQVIPIMAIESIIVAYS
Sbjct: 301  SARLSMGMDMMLMSGKERTKKEWEDLLRKANFTSHQVIPIMAIESIIVAYS 351


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  275 bits (704), Expect = 1e-71
 Identities = 137/338 (40%), Positives = 221/338 (65%), Gaps = 3/338 (0%)
 Frame = -3

Query: 1105 QAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKSTSIDRFHHFMR 929
            QA++WKH++GFAE+++L+S V L I D IHN+G P+TLS+L + LP++   +DR +  MR
Sbjct: 14   QAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDRLYRVMR 73

Query: 928  YLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLGPYVMLQTHPEEFSVWSHVIN- 752
            YLVHM+LF    D+  +  KY L P +K L+ G +KS+   ++L    +  S W H+   
Sbjct: 74   YLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPWHHLKEG 133

Query: 751  VLDGKKPYWESNDTSMYAKTEGDPEMNELLNDAMTSHSTFMLPALVSGLMKENVLDGVAS 572
            +++    + ++   S++     +P+ N++ N+ M   S  +  ALV+     N+  G+ +
Sbjct: 134  LVNNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNEC--GNIFQGIQT 191

Query: 571  IVDVGGNSGIVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMFTSVPNADAIL 392
            +VDVGG +G   K +  AFPH+KCS+ DL HVI      P +  + G+MF S+P+ADAIL
Sbjct: 192  LVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPSADAIL 251

Query: 391  LKSTLHNYEDDDCIKILRKAKEAIPSSGGKVILVEIVVDIENLPQFTSARLSMEVDMML- 215
            +K  LH++ DD+CI+IL+K KEA+P  GGKVI+V+IV+D+E+   ++  RL++++DMML 
Sbjct: 252  MKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDLDMMLN 311

Query: 214  MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 101
              GKERT++EW+ L+  A F  +++  I A++S+I A+
Sbjct: 312  TEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAF 349


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  260 bits (665), Expect = 4e-67
 Identities = 135/344 (39%), Positives = 224/344 (65%), Gaps = 4/344 (1%)
 Frame = -3

Query: 1120 DEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNGP-VTLSQLVTHLPLKSTSIDRF 944
            D + +QA++W  ++GFAE+++L+  V L + +IIHN+G  +TLS+L + LP +  + D  
Sbjct: 6    DNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNEDAL 65

Query: 943  HHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLGPYVMLQTHPEEFSVWS 764
            +  MRYLVHM+LFT ++  I  E +Y L P +K LV G  K +   ++  T  +  + W 
Sbjct: 66   YRVMRYLVHMKLFTKAS--IDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWH 123

Query: 763  HVINVLDGKK--PYWESNDTSMYAKTEGDPEMNELLNDAMTSHSTFMLPALVSGLMKENV 590
            ++ + L G+    + ++  T+++      PE N+L N+AM + S  ++ ALV      N+
Sbjct: 124  YLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKEC--GNI 181

Query: 589  LDGVASIVDVGGNSGIVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMFTSVP 410
             +G+ ++VDVGG +G   + I +AFPH+KC+V DL HVI       ++  VAGDMF  +P
Sbjct: 182  FNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIP 241

Query: 409  NADAILLKSTLHNYEDDDCIKILRKAKEAIPSSGGKVILVEIVVDIENLPQFTSARLSME 230
             ADAI++K  LH+++D +CI+IL++ KEA+P  GGKVI+V+IV+++++   +T  RL+++
Sbjct: 242  KADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLD 301

Query: 229  VDMML-MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 101
            +DMML  GGKERT++EW+ L+  A +  H++  I A++S+I AY
Sbjct: 302  LDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAY 345


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  259 bits (661), Expect = 1e-66
 Identities = 131/348 (37%), Positives = 224/348 (64%), Gaps = 6/348 (1%)
 Frame = -3

Query: 1126 NDDEIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLKSTSID 950
            N + + +QA++W  ++GFA++++L+S V L + +IIHN+G P+TLS+L  HLP +  + D
Sbjct: 5    NKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQD 64

Query: 949  RFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLGPYVMLQTHPEEFSV 770
              +  +RYLVHM+LFT S+  I  E +Y L P +K LV G  K +   ++  T  +  + 
Sbjct: 65   ALYRVLRYLVHMKLFTKSS--IDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAP 122

Query: 769  WSH----VINVLDGKKPYWESNDTSMYAKTEGDPEMNELLNDAMTSHSTFMLPALVSGLM 602
            W +    ++N       + ++  T+++      PE N+L N+ M + +  ++ ALV    
Sbjct: 123  WHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKEC- 181

Query: 601  KENVLDGVASIVDVGGNSGIVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAGDMF 422
              ++ DG+ +IVDVGG +G   + I  AFPH+KC+V DL HVI       +++ + GDMF
Sbjct: 182  -SSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMF 240

Query: 421  TSVPNADAILLKSTLHNYEDDDCIKILRKAKEAIPSSGGKVILVEIVVDIENLPQFTSAR 242
              +PNADAI++K  LH+++D +CI+IL++ K+A+P  GGKVI+++I++D+++   +T  R
Sbjct: 241  KYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMR 300

Query: 241  LSMEVDMML-MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 101
            L++++DMML  GGKERT++EW+ L+  A +  +++  I A++S+I AY
Sbjct: 301  LTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAY 348


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
            flavum subsp. glaucum]
          Length = 348

 Score =  254 bits (648), Expect = 4e-65
 Identities = 132/351 (37%), Positives = 222/351 (63%), Gaps = 7/351 (1%)
 Frame = -3

Query: 1132 FQNDD---EIKAQAQVWKHMFGFAETIMLRSTVSLGIPDIIHNNG-PVTLSQLVTHLPLK 965
            FQ  D   +IKAQA VWK ++GFA++++LR  V LGI DII NN  P+ L+ L + LP+ 
Sbjct: 4    FQGKDASVDIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPIS 63

Query: 964  STSIDRFHHFMRYLVHMQLFTISTDQITKEDKYELTPASKLLVHGHQKSLGPYVMLQTHP 785
            + ++D     +RYLV M+L + + D     DKY L P +KL++   ++S+ P V+  T  
Sbjct: 64   NVNLDNLFRILRYLVKMELLSYAAD-----DKYALEPIAKLVLRNEKRSMVPMVLGMTQK 118

Query: 784  EEFSVWSHVINVL--DGKKPYWESNDTSMYAKTEGDPEMNELLNDAMTSHSTFMLPALVS 611
            +  + W  + + L  +G   + ++   +++   EG P+ ++L N+ M   +  +  +L+S
Sbjct: 119  DFMTPWHSMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 178

Query: 610  GLMKENVLDGVASIVDVGGNSGIVAKGIVDAFPHVKCSVMDLNHVIERVIKNPKLDYVAG 431
            G   +++   + S+VDVGG +G   K I  AFPH+KC++ DL HVI     +P ++ + G
Sbjct: 179  G--SKDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGG 236

Query: 430  DMFTSVPNADAILLKSTLHNYEDDDCIKILRKAKEAIPSSGGKVILVEIVVDIENLPQFT 251
            DMF  +P+A AILLK  LH++ D+D IKIL++ ++A+P  GGKVI+V++ +D ++  + +
Sbjct: 237  DMFKGMPSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELS 296

Query: 250  SARLSMEVDMML-MGGKERTKKEWEDLLRKASFTYHQVIPIMAIESIIVAY 101
            S RL +++DM++  GGKERTK++WE L++ A F+  ++  I AI+S+I  +
Sbjct: 297  STRLILDIDMLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVF 347