BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis23_contig00000049
         (3536 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002269566.1| PREDICTED: E3 ubiquitin-protein ligase HERC2...   450   e-123
emb|CBI32027.3| unnamed protein product [Vitis vinifera]              446   e-122
ref|XP_003519776.1| PREDICTED: probable E3 ubiquitin-protein lig...   428   e-117
ref|XP_003517766.1| PREDICTED: probable E3 ubiquitin-protein lig...   420   e-114
ref|XP_002321756.1| predicted protein [Populus trichocarpa] gi|2...   414   e-112

>ref|XP_002269566.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Vitis vinifera]
          Length = 400

 Score =  450 bits (1158), Expect = e-123
 Identities = 233/377 (61%), Positives = 277/377 (73%), Gaps = 1/377 (0%)
 Frame = -2

Query: 1813 VWSWGAGTEGQLGTTKLQDENHPQLIHSLSFTPKH-VSYLACGGAHVIALSREDGKVLSW 1637
            +WSWGAGT+GQLGTT+L+DE+ PQL+    F+    +S LACGGAHV+AL+  DGKV++W
Sbjct: 14   IWSWGAGTDGQLGTTRLEDEHLPQLLDLPRFSSAGPISLLACGGAHVLALTT-DGKVVTW 72

Query: 1636 GRGTSGQLGHGDMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVFTCGDGTFGQL 1457
            GRGTSGQLGHGDM + L P  VK LESFV+++VSAGW+HSGFVS+TG +FTCGDG+FGQL
Sbjct: 73   GRGTSGQLGHGDMVSSLQPKHVKLLESFVMTHVSAGWSHSGFVSDTGCLFTCGDGSFGQL 132

Query: 1456 GHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMD 1277
            GHGD +S C P++V YF S+HV+QI CGMRH+LVLLK   GD +YGFGSGKRGQLG SMD
Sbjct: 133  GHGDCKSHCSPVKVSYFDSKHVDQIACGMRHSLVLLK---GDQVYGFGSGKRGQLGVSMD 189

Query: 1276 MITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGMSDACLPLLPSS 1097
              T+S+++PQ   G + V I  I ANGD SAAL+ +GHLYTWG+GF   SD   P    S
Sbjct: 190  N-TRSISVPQATVGLEGVEIISIDANGDHSAALSANGHLYTWGRGFGSTSDVHCPRCLPS 248

Query: 1096 ALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDC 917
            +L FT+VALGWNH L+LT  G VFMLGGN HG+L  PQK       S       G E+  
Sbjct: 249  SLCFTQVALGWNHMLVLTGGGEVFMLGGNRHGVLGDPQKGSQVKHLSVIEVFLHGPEES- 307

Query: 916  RLSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMIWGWGEHGQLGLGNEEDQIAPQIV 737
            R   LEKV GLDG+  V IAAGAEHSALVTEN  I  WGWGEHGQLGLGN  DQI+PQ V
Sbjct: 308  REENLEKVQGLDGLNVVQIAAGAEHSALVTENRVIKTWGWGEHGQLGLGNTYDQISPQAV 367

Query: 736  SLDAKLLKIQTLFKVYC 686
             L  +L    T+ KVYC
Sbjct: 368  RLGHRLPGQHTMIKVYC 384


>emb|CBI32027.3| unnamed protein product [Vitis vinifera]
          Length = 405

 Score =  446 bits (1147), Expect = e-122
 Identities = 230/380 (60%), Positives = 279/380 (73%), Gaps = 4/380 (1%)
 Frame = -2

Query: 1813 VWSWGAGTEGQLGTTKLQDENHPQLIHSLSFTPKH-VSYLACGGAHVIALSREDGKVLSW 1637
            +WSWGAGT+GQLGTT+L+DE+ PQL+    F+    +S LACGGAHV+AL+  DGKV++W
Sbjct: 22   IWSWGAGTDGQLGTTRLEDEHLPQLLDLPRFSSAGPISLLACGGAHVLALTT-DGKVVTW 80

Query: 1636 GRGTSGQLGHGDMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVFTCGDGTFGQL 1457
            GRGTSGQLGHGDM + L P  VK LESFV+++VSAGW+HSGFVS+TG +FTCGDG+FGQL
Sbjct: 81   GRGTSGQLGHGDMVSSLQPKHVKLLESFVMTHVSAGWSHSGFVSDTGCLFTCGDGSFGQL 140

Query: 1456 GHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGN--SGDALYGFGSGKRGQLGTS 1283
            GHGD +S C P++V YF S+HV+QI CGMRH+LVLLKG   SGD +YGFGSGKRGQLG S
Sbjct: 141  GHGDCKSHCSPVKVSYFDSKHVDQIACGMRHSLVLLKGTGLSGDQVYGFGSGKRGQLGVS 200

Query: 1282 MDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGMSDACLPLLP 1103
            MD  T+S+++PQ   G + V I  I ANGD SAAL+ +GHLYTWG+GF   SD   P   
Sbjct: 201  MDN-TRSISVPQATVGLEGVEIISIDANGDHSAALSANGHLYTWGRGFGSTSDVHCPRCL 259

Query: 1102 SSALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKLRLKTCSSNQSTGCQGSED 923
             S+L FT+VALGWNH L+LT  G VFMLGGN HG+L  PQK           +  +   +
Sbjct: 260  PSSLCFTQVALGWNHMLVLTGGGEVFMLGGNRHGVLGDPQK----------GSQVKHLSE 309

Query: 922  DCRLSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIM-IWGWGEHGQLGLGNEEDQIAP 746
            + R   LEKV GLDG+  V IAAGAEHSALVT N  ++  WGWGEHGQLGLGN  DQI+P
Sbjct: 310  ESREENLEKVQGLDGLNVVQIAAGAEHSALVTGNILVIKTWGWGEHGQLGLGNTYDQISP 369

Query: 745  QIVSLDAKLLKIQTLFKVYC 686
            Q V L  +L    T+ KVYC
Sbjct: 370  QAVRLGHRLPGQHTMIKVYC 389


>ref|XP_003519776.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Glycine
            max]
          Length = 388

 Score =  428 bits (1100), Expect = e-117
 Identities = 222/377 (58%), Positives = 270/377 (71%), Gaps = 1/377 (0%)
 Frame = -2

Query: 1813 VWSWGAGTEGQLGTTKLQDENHPQLIHSLSFTPKHVSYLACGGAHVIALSREDGKVLSWG 1634
            VWSWGAGTEGQLGT  LQDE+ PQL+H  S +   +S LACGGAHVIAL+   GK LSWG
Sbjct: 20   VWSWGAGTEGQLGTKILQDEHFPQLLHQFSLSS--ISSLACGGAHVIALTSA-GKALSWG 76

Query: 1633 RGTSGQLGHGDM-KNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVFTCGDGTFGQL 1457
            RG SGQLGHG++  N LYP  V  L+ + +++VSAGW+HSGFVS+TG VFTCGDG+FGQL
Sbjct: 77   RGNSGQLGHGEVVSNTLYPKAVTSLDGYFITHVSAGWSHSGFVSDTGFVFTCGDGSFGQL 136

Query: 1456 GHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMD 1277
            GHGD  S C P++V  F   HV Q+ CGMRH+LVLLKGN    +YGFGSGKRGQLG S D
Sbjct: 137  GHGDNASHCSPVKVSCFVDLHVAQVACGMRHSLVLLKGNQ---VYGFGSGKRGQLGVSND 193

Query: 1276 MITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGMSDACLPLLPSS 1097
             + KS+N+P+V+ GF+ V I    ANGD SAA++VDGH+YTWG+GF G  DA +P   +S
Sbjct: 194  RV-KSVNVPKVVSGFEGVEIVGTAANGDHSAAVSVDGHVYTWGRGFKGFEDAHVPQCLNS 252

Query: 1096 ALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDC 917
            +L FTKVALGWNHAL ++  G V+MLGGNH G+LS  Q +           G      D 
Sbjct: 253  SLNFTKVALGWNHALAMSGEGEVYMLGGNHLGVLSDLQNI-----------GPAKHFPDL 301

Query: 916  RLSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMIWGWGEHGQLGLGNEEDQIAPQIV 737
            R   LEKV GLDG K  DIAAGAEHS +VTE+G+I  WGWGEHGQLGLG+  DQ +P  V
Sbjct: 302  REFNLEKVPGLDGTKITDIAAGAEHSVIVTEHGEIKTWGWGEHGQLGLGDTRDQTSPVTV 361

Query: 736  SLDAKLLKIQTLFKVYC 686
            SLD  L +  ++ +V+C
Sbjct: 362  SLDYDLNEAASI-RVFC 377



 Score = 72.8 bits (177), Expect = 6e-10
 Identities = 68/256 (26%), Positives = 108/256 (42%), Gaps = 43/256 (16%)
 Frame = -2

Query: 1348 KGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVD 1169
            +  S   ++ +G+G  GQLGT    I +  + PQ++  F   +I+ +   G    ALT  
Sbjct: 13   RDESEQRVWSWGAGTEGQLGTK---ILQDEHFPQLLHQFSLSSISSLACGGAHVIALTSA 69

Query: 1168 GHLYTWGKGFHG-------MSDACLPLLPSS--ALKFTKVALGWNHALLLTDCGTVFMLG 1016
            G   +WG+G  G       +S+   P   +S      T V+ GW+H+  ++D G VF  G
Sbjct: 70   GKALSWGRGNSGQLGHGEVVSNTLYPKAVTSLDGYFITHVSAGWSHSGFVSDTGFVFTCG 129

Query: 1015 GNHHGMLS--------SPQKL-----------------RLKTCSSNQ----STGCQG--- 932
                G L         SP K+                  L     NQ     +G +G   
Sbjct: 130  DGSFGQLGHGDNASHCSPVKVSCFVDLHVAQVACGMRHSLVLLKGNQVYGFGSGKRGQLG 189

Query: 931  -SEDDCR-LSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMIWGWGEHGQLGLGNEED 758
             S D  + +++ + VSG +G++ V  AA  +HSA V+ +G +  WG G  G       ED
Sbjct: 190  VSNDRVKSVNVPKVVSGFEGVEIVGTAANGDHSAAVSVDGHVYTWGRGFKG------FED 243

Query: 757  QIAPQIVSLDAKLLKI 710
               PQ ++      K+
Sbjct: 244  AHVPQCLNSSLNFTKV 259


>ref|XP_003517766.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2-like [Glycine
            max]
          Length = 388

 Score =  420 bits (1080), Expect = e-114
 Identities = 221/377 (58%), Positives = 268/377 (71%), Gaps = 1/377 (0%)
 Frame = -2

Query: 1813 VWSWGAGTEGQLGTTKLQDENHPQLIHSLSFTPKHVSYLACGGAHVIALSREDGKVLSWG 1634
            VWSWGAGTEGQLGT  LQDE+ PQL+H  S +   +S LACGGAHVIAL+   GKVLSWG
Sbjct: 20   VWSWGAGTEGQLGTKILQDEHFPQLLHQPSLSS--ISSLACGGAHVIALTSA-GKVLSWG 76

Query: 1633 RGTSGQLGHG-DMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVFTCGDGTFGQL 1457
            RG SGQLGHG  + N LYP  V  L+ + +++VSAGW HSGFVS+ G VFTCGDG+FGQL
Sbjct: 77   RGNSGQLGHGLVVSNSLYPKAVTSLDGYFITHVSAGWGHSGFVSDNGCVFTCGDGSFGQL 136

Query: 1456 GHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSMD 1277
            GHGD  S C P++V  F  +HVEQ+ CGMRH+LVLLKGN    +YGFGSGKRGQLG S D
Sbjct: 137  GHGDHASHCSPVKVSCFVDQHVEQVACGMRHSLVLLKGNQ---VYGFGSGKRGQLGVSND 193

Query: 1276 MITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGMSDACLPLLPSS 1097
             + KS+N+P+V+ GF+ V IA I ANGD SAA++VDGH+YTWG+GF G  DA +P   +S
Sbjct: 194  RV-KSVNVPKVVSGFEGVEIAGIAANGDHSAAVSVDGHVYTWGRGFKGFEDARVPQCLNS 252

Query: 1096 ALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDDC 917
            +L FTKVALGWNHAL ++  G V MLGGNH G+LS  Q +       +            
Sbjct: 253  SLNFTKVALGWNHALAMSGEGEVCMLGGNHLGVLSDLQNISPAKHLPH-----------L 301

Query: 916  RLSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMIWGWGEHGQLGLGNEEDQIAPQIV 737
            R   LEKV GLDG K  DIA GAEHS +VTE+G+I  WGWGEHGQLGLG+  D+I+P  V
Sbjct: 302  REVNLEKVPGLDGTKITDIATGAEHSVIVTEHGEIKTWGWGEHGQLGLGDTRDRISPVTV 361

Query: 736  SLDAKLLKIQTLFKVYC 686
            SL   L +  ++  V+C
Sbjct: 362  SLGYDLNEAASVI-VFC 377



 Score = 70.9 bits (172), Expect = 2e-09
 Identities = 68/256 (26%), Positives = 108/256 (42%), Gaps = 43/256 (16%)
 Frame = -2

Query: 1348 KGNSGDALYGFGSGKRGQLGTSMDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVD 1169
            K  S   ++ +G+G  GQLGT    I +  + PQ++      +I+ +   G    ALT  
Sbjct: 13   KDESEQRVWSWGAGTEGQLGTK---ILQDEHFPQLLHQPSLSSISSLACGGAHVIALTSA 69

Query: 1168 GHLYTWGKGFHG-------MSDACLPLLPSS--ALKFTKVALGWNHALLLTDCGTVFMLG 1016
            G + +WG+G  G       +S++  P   +S      T V+ GW H+  ++D G VF  G
Sbjct: 70   GKVLSWGRGNSGQLGHGLVVSNSLYPKAVTSLDGYFITHVSAGWGHSGFVSDNGCVFTCG 129

Query: 1015 GNHHGMLS--------SPQKL-----------------RLKTCSSNQ----STGCQG--- 932
                G L         SP K+                  L     NQ     +G +G   
Sbjct: 130  DGSFGQLGHGDHASHCSPVKVSCFVDQHVEQVACGMRHSLVLLKGNQVYGFGSGKRGQLG 189

Query: 931  -SEDDCR-LSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMIWGWGEHGQLGLGNEED 758
             S D  + +++ + VSG +G++   IAA  +HSA V+ +G +  WG G  G       ED
Sbjct: 190  VSNDRVKSVNVPKVVSGFEGVEIAGIAANGDHSAAVSVDGHVYTWGRGFKG------FED 243

Query: 757  QIAPQIVSLDAKLLKI 710
               PQ ++      K+
Sbjct: 244  ARVPQCLNSSLNFTKV 259


>ref|XP_002321756.1| predicted protein [Populus trichocarpa] gi|222868752|gb|EEF05883.1|
            predicted protein [Populus trichocarpa]
          Length = 390

 Score =  414 bits (1063), Expect = e-112
 Identities = 216/378 (57%), Positives = 262/378 (69%), Gaps = 2/378 (0%)
 Frame = -2

Query: 1813 VWSWGAGTEGQLGTTKLQDENHPQLIHSLSF--TPKHVSYLACGGAHVIALSREDGKVLS 1640
            +WSWGAGTEGQLGT KL+DE  PQL+H L F  + + +S LACGGAHVIAL+   G+V +
Sbjct: 16   IWSWGAGTEGQLGTGKLEDEYLPQLLH-LPFLSSAESISTLACGGAHVIALT-SGGRVFT 73

Query: 1639 WGRGTSGQLGHGDMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVFTCGDGTFGQ 1460
            WGRGT+GQLGHG+M N L+P PV  L+S V+++VSAGW+HSGFVS+ G +FTCGDG+FGQ
Sbjct: 74   WGRGTTGQLGHGEMLNSLHPKPVSSLQSCVITHVSAGWSHSGFVSDIGLLFTCGDGSFGQ 133

Query: 1459 LGHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSM 1280
            LG G+++SQ  P++V YF +  V QI CGMRH+LVLL GN    +YGFGSGKRGQLG S 
Sbjct: 134  LGQGNYRSQSTPVKVDYFANNFVNQIACGMRHSLVLLNGNQ---VYGFGSGKRGQLGISR 190

Query: 1279 DMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGMSDACLPLLPS 1100
            D  TKS+NLPQV  G + V I  I ANGD SAA++ DGHLYTWG+GF G SDA  P L  
Sbjct: 191  DK-TKSINLPQVTCGLEDVQIVSISANGDHSAAISADGHLYTWGRGFAGASDANFPQLSL 249

Query: 1099 SALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKLRLKTCSSNQSTGCQGSEDD 920
            S+L+ TK ALGWNH LLLT    V MLGGN+HG+L          C   + +  +   +D
Sbjct: 250  SSLRCTKAALGWNHGLLLTGDEEVLMLGGNYHGVL----------CDLEKMSAVKHRPED 299

Query: 919  CRLSILEKVSGLDGIKAVDIAAGAEHSALVTENGKIMIWGWGEHGQLGLGNEEDQIAPQI 740
               + L +V GLDG+K V IAAGAEHSAL         WGWGEHGQLGLGN  DQ  PQ 
Sbjct: 300  SPGTALNEVIGLDGVKVVKIAAGAEHSALT--------WGWGEHGQLGLGNTNDQTIPQT 351

Query: 739  VSLDAKLLKIQTLFKVYC 686
            VSL   +   +    VYC
Sbjct: 352  VSLIPDIQNKEASLNVYC 369