BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00030308
         (1021 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002510140.1| cytochrome P450, putative [Ricinus communis]...   313   4e-83
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]...   311   2e-82
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   311   2e-82
ref|XP_002285061.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   306   4e-81
emb|CAN60309.1| hypothetical protein VITISV_015004 [Vitis vinifera]   306   7e-81

>ref|XP_002510140.1| cytochrome P450, putative [Ricinus communis]
            gi|223550841|gb|EEF52327.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 504

 Score =  313 bits (802), Expect = 4e-83
 Identities = 165/338 (48%), Positives = 223/338 (65%)
 Frame = -3

Query: 1019 NRPHLLMGKHLNYNYTTITFVPYGDLWRNLRRLATLEILSSTRLQVSSNIRKEEVSVVVR 840
            +RP    GK+L+Y+YTTI    YGDLWRNLRRLAT+EILS+ RL +   IR+EEV ++V+
Sbjct: 98   DRPRRQAGKYLHYDYTTIGAANYGDLWRNLRRLATVEILSTNRLNMFHGIRQEEVRMLVK 157

Query: 839  EMFRGYNGNFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQEKAKEKLMDLKSTFC 660
             +F+       KVE++S    L FN+IMR++  + +    E+   E+A +    ++ TF 
Sbjct: 158  NLFQSAGQVSAKVEMKSRLVGLSFNIIMRMVAGKRYFGS-EVKDVEEATQFHDVIRETFV 216

Query: 659  TTGIFGLGDFFPFLKWLDSWRVEKIMIKLFKKRDKFLHAVIDDCRTRRSNSSLDASTATR 480
             +G   LGDFFP ++WLD   +EK ++   K  D     +ID+ R +R +          
Sbjct: 217  LSGAANLGDFFPLIRWLDYRGIEKRLVSARKNMDLLFQRLIDEHRHKRGSCL-------- 268

Query: 479  EYRKEEKDNKSIIDVLLSLQETQPAYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLN 300
                E+K  K++IDV+LSLQE QP YY+D+IIKG+I  ML AGT+ S  T EWAMSLLLN
Sbjct: 269  ----EDKSCKTMIDVVLSLQEFQPEYYSDEIIKGLIMAMLTAGTDTSAVTIEWAMSLLLN 324

Query: 299  HPEVLNKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCT 120
            HP+ L KAR E++ +VG DRL++E DL KL YL  IINETLRL+   P   PH+S+DDC 
Sbjct: 325  HPKALTKARAELDIHVGQDRLVDEQDLPKLQYLHCIINETLRLFPAAPLLVPHKSSDDCK 384

Query: 119  IGGYNIPKGTLLFVNILALHRDPKAWSEPMMFKPERFE 6
            IGG++IP+GT+L VN  ALHRDPK W +P  F+PERFE
Sbjct: 385  IGGFDIPQGTVLSVNAWALHRDPKIWEDPNSFRPERFE 422


>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
            gi|223550831|gb|EEF52317.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 500

 Score =  311 bits (797), Expect = 2e-82
 Identities = 160/339 (47%), Positives = 230/339 (67%), Gaps = 1/339 (0%)
 Frame = -3

Query: 1019 NRPHLLMGKHLNYNYTTITFVPYGDLWRNLRRLATLEILSSTRLQVSSNIRKEEVSVVVR 840
            NRP LL GKH+ YNYT++ + PYGDLWRNLR+++ LEILSS RLQ+ S+IR++EV ++++
Sbjct: 93   NRPRLLAGKHIGYNYTSLAWAPYGDLWRNLRKVSALEILSSHRLQLLSSIRRDEVKLLIQ 152

Query: 839  EMFRGYNGNFTKVELRSIFCKLIFNMIMRIIGMQPFLRED-EMVGQEKAKEKLMDLKSTF 663
             +FR       KV+++S F +L+ N++MR+I  + +  E+ E V  E+AK     ++ TF
Sbjct: 153  RLFRNNKDCREKVDMKSAFFELMLNVMMRMIAGKRYYGENVEQV--EEAKRFREIVRETF 210

Query: 662  CTTGIFGLGDFFPFLKWLDSWRVEKIMIKLFKKRDKFLHAVIDDCRTRRSNSSLDASTAT 483
               G   +GDF P +  +     EK + +L K+RD F+  +ID+ R R +  S       
Sbjct: 211  LAAGTSNMGDFLPLVAVVGGQ--EKRLKELGKRRDGFIQDLIDEHRKRMAACS------- 261

Query: 482  REYRKEEKDNKSIIDVLLSLQETQPAYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLL 303
                  E+ NK++I+VLLSLQE++P YYTD+ IK ++ V+L AGT+ S  T EWAMSLL+
Sbjct: 262  -----SEERNKTMIEVLLSLQESEPEYYTDETIKSLMLVLLAAGTDTSAATMEWAMSLLV 316

Query: 302  NHPEVLNKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDC 123
            N+PE L KA+TEI+  +G+DRL+NE+D  KLPYL  IINE +R+Y  GP   PHES+++C
Sbjct: 317  NNPEALKKAQTEIDSVIGHDRLINESDTSKLPYLNCIINEVMRMYPAGPLLVPHESSEEC 376

Query: 122  TIGGYNIPKGTLLFVNILALHRDPKAWSEPMMFKPERFE 6
             IGGY +P GT+L VN+ ++  DP+ W EP  FKPERFE
Sbjct: 377  FIGGYRVPAGTMLLVNLWSIQNDPRVWEEPRNFKPERFE 415


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  311 bits (796), Expect = 2e-82
 Identities = 160/338 (47%), Positives = 228/338 (67%)
 Frame = -3

Query: 1019 NRPHLLMGKHLNYNYTTITFVPYGDLWRNLRRLATLEILSSTRLQVSSNIRKEEVSVVVR 840
            NRP L++GKH   NYTT+ +  YG  WRNLRR++ LE+ S  R+Q+ S IR +EV  +VR
Sbjct: 96   NRPRLMIGKHSGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVR 155

Query: 839  EMFRGYNGNFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQEKAKEKLMDLKSTFC 660
             +  GY   + KVE++S+F +L  N++MR+I  + +  E  +   E+A++    ++ TF 
Sbjct: 156  RLVAGYE--YHKVEMKSVFFELTLNIMMRMIAGKRYYGE-HVADLEQARKFKEIVEETFE 212

Query: 659  TTGIFGLGDFFPFLKWLDSWRVEKIMIKLFKKRDKFLHAVIDDCRTRRSNSSLDASTATR 480
             +G   +GDF P L+W+    +EK +I+L  KRD+FL  +I + R  R NS        +
Sbjct: 213  LSGASNVGDFLPLLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVNS--------K 264

Query: 479  EYRKEEKDNKSIIDVLLSLQETQPAYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLN 300
             +  EEK  K++IDVLLSLQET+P YYTD++I+G+I ++  AGT+ S  T EWAMSLLLN
Sbjct: 265  SFPSEEK-KKTLIDVLLSLQETEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLN 323

Query: 299  HPEVLNKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCT 120
            +P+++ KA++EI+  +   R ++E+DL+KLPYL  IINETLR+Y  GP   PHES++DC 
Sbjct: 324  NPDLIKKAQSEIDLQIEPGRPIDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCE 383

Query: 119  IGGYNIPKGTLLFVNILALHRDPKAWSEPMMFKPERFE 6
            IGGYNIP GT+L VN+ A+  DP  W EP  FKPERFE
Sbjct: 384  IGGYNIPCGTMLLVNLWAIQNDPNLWKEPRKFKPERFE 421


>ref|XP_002285061.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 508

 Score =  306 bits (785), Expect = 4e-81
 Identities = 153/338 (45%), Positives = 217/338 (64%)
 Frame = -3

Query: 1019 NRPHLLMGKHLNYNYTTITFVPYGDLWRNLRRLATLEILSSTRLQVSSNIRKEEVSVVVR 840
            NRP LL GKHL+Y+YTT+   PYG  WRNLRRL TLEI S+ RL +   IR++EV  ++R
Sbjct: 100  NRPRLLAGKHLHYDYTTLGVAPYGQHWRNLRRLTTLEIFSTNRLNMFLGIRQDEVKFLLR 159

Query: 839  EMFRGYNGNFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQEKAKEKLMDLKSTFC 660
             +F+     F +VE++S   +L FN+I RI+  + +   D    +E ++ + + +K    
Sbjct: 160  NLFQRSGQGFARVEMKSRLSELSFNIITRIVAGKRYFGTDVEDFEEASRFRDI-IKEILE 218

Query: 659  TTGIFGLGDFFPFLKWLDSWRVEKIMIKLFKKRDKFLHAVIDDCRTRRSNSSLDASTATR 480
            T+G    GDF PFL+W D   ++K ++ L ++ D FL  +ID+ R   ++          
Sbjct: 219  TSGASNAGDFLPFLQWFDFQGLKKRVLALQRRTDAFLQGLIDEGRNHNNS---------- 268

Query: 479  EYRKEEKDNKSIIDVLLSLQETQPAYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLN 300
             YR+E    K+ +D +L+LQ ++P YY D IIKG+I  +L AGT+ S  T EWAMSLLLN
Sbjct: 269  -YRQERGKTKTFVDSMLALQNSEPEYYADHIIKGMILTLLTAGTDTSAVTMEWAMSLLLN 327

Query: 299  HPEVLNKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCT 120
            HP VL+K +TE++C +G+ RL+ E DL  LPYL  I+NETLRL+   P    HES+DDC+
Sbjct: 328  HPTVLDKVKTELDCKIGHQRLVEEPDLSDLPYLRAIVNETLRLFPAAPLLVAHESSDDCS 387

Query: 119  IGGYNIPKGTLLFVNILALHRDPKAWSEPMMFKPERFE 6
            IGGY++  GT+L VN  A+HRD K W +P  F+PERFE
Sbjct: 388  IGGYDVRGGTMLLVNAWAIHRDAKVWEDPTSFRPERFE 425


>emb|CAN60309.1| hypothetical protein VITISV_015004 [Vitis vinifera]
          Length = 990

 Score =  306 bits (783), Expect = 7e-81
 Identities = 154/338 (45%), Positives = 220/338 (65%)
 Frame = -3

Query: 1019 NRPHLLMGKHLNYNYTTITFVPYGDLWRNLRRLATLEILSSTRLQVSSNIRKEEVSVVVR 840
            NRP  +MGK++ Y+YT ++  PYGD WRNLRRL+ +EI +S RL +   IR++E+  ++ 
Sbjct: 581  NRPKFVMGKYIGYDYTVVSLAPYGDHWRNLRRLSAVEIFASNRLNLFLGIRRDEIKQLLL 640

Query: 839  EMFRGYNGNFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQEKAKEKLMDLKSTFC 660
             + R    NF KVEL+S+F +L+ N+ MR++  + F   D M   E+A+E     K    
Sbjct: 641  RLSRNSVENFAKVELKSMFSELLLNITMRMVAGKRFYG-DNMKDVEEAREFREISKEILE 699

Query: 659  TTGIFGLGDFFPFLKWLDSWRVEKIMIKLFKKRDKFLHAVIDDCRTRRSNSSLDASTATR 480
              G    GDF P L+W+D     K  ++L KK D FL  ++D+CR+ +            
Sbjct: 700  FXGTSNPGDFXPILQWIDYQGYNKRALRLGKKMDVFLQGLLDECRSNK------------ 747

Query: 479  EYRKEEKDNKSIIDVLLSLQETQPAYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLN 300
              R + ++  ++ID LLSLQE++P YYTD+IIKG+I  M + G + +  T EWAMSLLLN
Sbjct: 748  --RSDLENRNTMIDHLLSLQESEPEYYTDEIIKGLIVAMQVGGADTTAVTIEWAMSLLLN 805

Query: 299  HPEVLNKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCT 120
            HPEVL KAR E++ ++G+D L++ETDL KL YL +II+E+LRL+   P   PH ST+DC 
Sbjct: 806  HPEVLKKARDELDTHIGHDCLIDETDLPKLQYLQSIISESLRLFPSTPLLVPHFSTEDCK 865

Query: 119  IGGYNIPKGTLLFVNILALHRDPKAWSEPMMFKPERFE 6
            +GG+++P GT+L VN  ALHRDPK W++P  FKPERFE
Sbjct: 866  LGGFDVPGGTMLLVNAWALHRDPKLWNDPTSFKPERFE 903



 Score =  284 bits (727), Expect = 2e-74
 Identities = 153/338 (45%), Positives = 208/338 (61%)
 Frame = -3

Query: 1019 NRPHLLMGKHLNYNYTTITFVPYGDLWRNLRRLATLEILSSTRLQVSSNIRKEEVSVVVR 840
            NRP L++ ++L Y YT+I   PYG+ WRNLRRL  LEI SS RL +   IRK+E+  ++R
Sbjct: 98   NRPRLMVSEYLGYKYTSIVSSPYGEHWRNLRRLCALEIFSSNRLNMFLGIRKDEIKHLLR 157

Query: 839  EMFRGYNGNFTKVELRSIFCKLIFNMIMRIIGMQPFLREDEMVGQEKAKEKLMDLKSTFC 660
             +      NF KVEL+S+F +L FN+I R++  + +  E      E+AK     ++ +F 
Sbjct: 158  RLGGDSRDNFAKVELKSLFSELTFNIITRMVAGKRYYGEGSDF--EEAKHFREIIRKSFL 215

Query: 659  TTGIFGLGDFFPFLKWLDSWRVEKIMIKLFKKRDKFLHAVIDDCRTRRSNSSLDASTATR 480
             +     GDF P L+W+D    EK M K  ++ D  L  +ID+    RSNS         
Sbjct: 216  LSAASNPGDFLPILRWMDYGGYEKKMAKNSRELDVILQGLIDE---HRSNS--------- 263

Query: 479  EYRKEEKDNKSIIDVLLSLQETQPAYYTDDIIKGVIQVMLIAGTEASFTTAEWAMSLLLN 300
              +K    N ++ID LLSLQ+++P YYTD IIKGV   ++ AGT+ +  T EWAMSLLLN
Sbjct: 264  --KKGLMGNNTMIDHLLSLQKSEPEYYTDQIIKGVTMNLVFAGTDTAAVTMEWAMSLLLN 321

Query: 299  HPEVLNKARTEIECNVGYDRLLNETDLDKLPYLLNIINETLRLYNLGPFGAPHESTDDCT 120
            HP+VL KA+ E++  VG +RLL E DL KL YL NII+ET RL    P   PH S+ +C 
Sbjct: 322  HPDVLKKAKVELDTXVGQERLLEEADLPKLHYLQNIISETFRLCPPAPLWLPHMSSANCQ 381

Query: 119  IGGYNIPKGTLLFVNILALHRDPKAWSEPMMFKPERFE 6
            +GG++IP+  +L VN   LHRDPK W +P  FKPERFE
Sbjct: 382  LGGFDIPRDXMLLVNSWTLHRDPKLWDDPTSFKPERFE 419