BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis21_contig00025899
         (1270 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   548   e-153
ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|2...   543   e-152
ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago...   534   e-149
ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   531   e-148
gb|AFK34722.1| unknown [Lotus japonicus]                              521   e-145

>ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial [Vitis
            vinifera] gi|297741744|emb|CBI32876.3| unnamed protein
            product [Vitis vinifera]
          Length = 424

 Score =  548 bits (1411), Expect = e-153
 Identities = 277/402 (68%), Positives = 319/402 (79%), Gaps = 3/402 (0%)
 Frame = -2

Query: 1197 MLRHTLRRTLKSCTSSVWIQSRKLTMLL---ETIPKENVDCXXXXXXXXXXXXARDLALK 1027
            MLRH +R    S     WI S+  T  +     + +E+VDC            AR+LALK
Sbjct: 1    MLRHAIRGLRNSRR---WISSKNFTSRVGVERAVARESVDCVVIGAGVVGIAVARELALK 57

Query: 1026 GRDVLVIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCSRGRKLLYQYCANHGVPHK 847
            GR+V+VIE A TFG+GTSSRNSEVIHAGIYYP NSLKAIFC+RGR+LLY+YC+ H VPHK
Sbjct: 58   GREVVVIEFASTFGTGTSSRNSEVIHAGIYYPRNSLKAIFCARGRELLYKYCSEHEVPHK 117

Query: 846  QIGKLIVATGSSEIQKLIVLLSRGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGI 667
            QIGKLIVAT SSE+ KL  L+ RG ENGV+GLRM+EGFEAM MEPEL+C++ALLSP SGI
Sbjct: 118  QIGKLIVATRSSEVPKLNDLMIRGNENGVDGLRMLEGFEAMEMEPELQCVRALLSPASGI 177

Query: 666  VDTHALMLSLVGEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQPEL 487
            VDTH+LMLSLVGEAENH  TF YNT V+GGHLEGD I +HIS+SK++E      P  PE+
Sbjct: 178  VDTHSLMLSLVGEAENHRTTFCYNTAVIGGHLEGDHICLHISESKDLENWDRRFPLHPEV 237

Query: 486  ILVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYARGCYFTLSNTKTPPFEHLIYPIPE 307
            IL+  LVVNSAGLS+P+LAK F GL+S VIP S+YARGCYF LS  KTPPF+HLIYPIPE
Sbjct: 238  ILMAKLVVNSAGLSAPALAKRFDGLNSTVIPASHYARGCYFVLSKMKTPPFKHLIYPIPE 297

Query: 306  DGGLGVHVTLDLDGHVKFGPDVEWIGELDCISSFLNKFDYSVSSDHVQKFYPDIRRYYPN 127
            DGGLGVHVTLDLDG +KFGPDVEWI   D ISSFLNKFDYSV +  V++FYP+IR+YYPN
Sbjct: 298  DGGLGVHVTLDLDGQIKFGPDVEWINGADDISSFLNKFDYSVCAKRVERFYPEIRKYYPN 357

Query: 126  LKEGSLEPGYAGIRPKLSGPGQPPVDFVIQGEDIHGVRGLIN 1
            LK+ SLEPGYAGIRPKLSG  Q  VDFVIQGEDIHGV GL+N
Sbjct: 358  LKDDSLEPGYAGIRPKLSGAQQSAVDFVIQGEDIHGVPGLVN 399


>ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|222854820|gb|EEE92367.1|
            predicted protein [Populus trichocarpa]
          Length = 430

 Score =  543 bits (1399), Expect = e-152
 Identities = 266/401 (66%), Positives = 325/401 (81%), Gaps = 3/401 (0%)
 Frame = -2

Query: 1194 LRHTLRRTLKSCTSSVWIQSRKLTMLL--ETIPKENVDCXXXXXXXXXXXXARDLALKGR 1021
            L+ TL+   ++ T S     + L+  +  E +P E +DC            AR+LA KGR
Sbjct: 5    LKQTLQSLKRTATISKAQAIKNLSTQIKKEDVPTEKLDCVVIGAGVVGIAIARELASKGR 64

Query: 1020 DVLVIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCSRGRKLLYQYCANHGVPHKQI 841
            +VLV++SA TFG+GTSSRNSEVIHAGIYYPP+SLKA+FC RGR+LLY+YC  HGVP KQI
Sbjct: 65   EVLVLDSASTFGTGTSSRNSEVIHAGIYYPPDSLKALFCVRGRELLYRYCYEHGVPCKQI 124

Query: 840  GKLIVATGSSEIQKLIVLLSRGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGIVD 661
            GKLIVATG  EI KL  L+ RG +NGV+ L+M+EGFEAM+MEPEL+C KALLSP SGIVD
Sbjct: 125  GKLIVATGPLEISKLNELMDRGTQNGVDNLKMLEGFEAMKMEPELQCKKALLSPVSGIVD 184

Query: 660  THALMLSLVGEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQPELIL 481
            +H+LMLSLVGEAEN+G TFSYN+TV+ GHLEG+ + ++I +SK++E   G  P  PEL+L
Sbjct: 185  SHSLMLSLVGEAENNGTTFSYNSTVISGHLEGNCLHLYIVESKDLENWNGDYPLHPELVL 244

Query: 480  VPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYARGCYFTLSNTKTPPFEHLIYPIPEDG 301
            VP LVVNSAGLSS +LAK F GLD+ +IPP ++ARGCYFTLS+TK PPFEHLIYPIPEDG
Sbjct: 245  VPKLVVNSAGLSSLALAKRFHGLDNGIIPPGFFARGCYFTLSSTKVPPFEHLIYPIPEDG 304

Query: 300  GLGVHVTLDLDGHVKFGPDVEWIGELDCISSFLNKFDYSVSSDHVQKFYPDIRRYYPNLK 121
            GLGVHVTLDLDGH+KFGPDVEWI  +D +SSFLNK+DYSVS+   ++FYP+IR+YYPNLK
Sbjct: 305  GLGVHVTLDLDGHLKFGPDVEWIDGIDDVSSFLNKYDYSVSASRAERFYPEIRKYYPNLK 364

Query: 120  EGSLEPGYAGIRPKLSGPGQPPVDFVI-QGEDIHGVRGLIN 1
            +GSL+PGY+GIRPK+SGP Q P+DFVI QGEDIHGV GL+N
Sbjct: 365  DGSLQPGYSGIRPKISGPRQSPIDFVIQQGEDIHGVPGLVN 405


>ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago truncatula]
            gi|355493920|gb|AES75123.1| L-2-hydroxyglutarate
            dehydrogenase [Medicago truncatula]
          Length = 483

 Score =  534 bits (1376), Expect = e-149
 Identities = 260/395 (65%), Positives = 318/395 (80%), Gaps = 25/395 (6%)
 Frame = -2

Query: 1110 TIPKENVDCXXXXXXXXXXXXARDLALKGRDVLVIESAPTFGSGTSSRNSEVIHAGIYYP 931
            ++P+E VDC            AR LALKGR+V+VIESAP+FG+GTSSRNSEV+HAGIYYP
Sbjct: 65   SVPRERVDCVVIGAGVVGIAVARALALKGREVIVIESAPSFGTGTSSRNSEVVHAGIYYP 124

Query: 930  PNSLKAIFCSRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKLIVLLSRGRENGVEGL 751
             +SLKAIFC +GR++LY+YCA H +PH+Q GKLIVAT SSEI KL V+L+ G +NGV+GL
Sbjct: 125  HHSLKAIFCVKGREMLYEYCAKHDIPHEQTGKLIVATRSSEIPKLSVILNHGIQNGVDGL 184

Query: 750  RMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLV--------------------- 634
            +MM+G +AM+MEPEL+C+KA+LSP SGIVD+H+LMLSLV                     
Sbjct: 185  KMMDGVDAMKMEPELQCVKAILSPLSGIVDSHSLMLSLVVCISSFYYHVVKLDLVLNINP 244

Query: 633  ----GEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLV 466
                GEAENHGATF+YN+TV+GGH+EG+ I +H+S++K++++  G    QPEL+L+P LV
Sbjct: 245  MLVQGEAENHGATFTYNSTVIGGHMEGNEICLHVSETKSLKEWNGKSSLQPELVLIPKLV 304

Query: 465  VNSAGLSSPSLAKSFKGLDSRVIPPSYYARGCYFTLSNTKTPPFEHLIYPIPEDGGLGVH 286
            VNSAGLS+ +LAK F GL+++VIPP+YYARGCYFTLSNTK  PF HLIYPIPEDGGLGVH
Sbjct: 305  VNSAGLSALALAKRFTGLENKVIPPAYYARGCYFTLSNTKASPFRHLIYPIPEDGGLGVH 364

Query: 285  VTLDLDGHVKFGPDVEWIGELDCISSFLNKFDYSVSSDHVQKFYPDIRRYYPNLKEGSLE 106
            VTLDL+G VKFGPDVEWI  +D ISSF NKFDYSV ++  +KFYP+IR+YYPNLK+GSLE
Sbjct: 365  VTLDLNGQVKFGPDVEWIDGVDDISSFQNKFDYSVQANRAEKFYPEIRKYYPNLKDGSLE 424

Query: 105  PGYAGIRPKLSGPGQPPVDFVIQGEDIHGVRGLIN 1
            PGY+GIRPKLSGP Q PVDFVIQGEDIHGV GLIN
Sbjct: 425  PGYSGIRPKLSGPCQSPVDFVIQGEDIHGVPGLIN 459


>ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial-like
            [Glycine max]
          Length = 410

 Score =  531 bits (1367), Expect = e-148
 Identities = 257/385 (66%), Positives = 313/385 (81%)
 Frame = -2

Query: 1155 SSVWIQSRKLTMLLETIPKENVDCXXXXXXXXXXXXARDLALKGRDVLVIESAPTFGSGT 976
            SS  IQS       +++PKE V+C            AR LALKGR+VLV+ESAPTFG+GT
Sbjct: 2    SSSTIQSNDNRTTWDSVPKERVECVVIGAGVVGIAVARALALKGREVLVVESAPTFGTGT 61

Query: 975  SSRNSEVIHAGIYYPPNSLKAIFCSRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKL 796
            SSRNSEVIHAGIYYP NS KAIFC RGR++LY+YC+ H +PHKQIGKLIVAT SSEI KL
Sbjct: 62   SSRNSEVIHAGIYYPLNSFKAIFCVRGREMLYEYCSKHDIPHKQIGKLIVATRSSEIPKL 121

Query: 795  IVLLSRGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLVGEAENH 616
              +L+ G +NGV+GL++++G EAM+MEPEL+C+KA+LSP +GIVD+H+LMLSLVGEAEN 
Sbjct: 122  NDILNCGIQNGVDGLKIVDGVEAMKMEPELQCVKAILSPLTGIVDSHSLMLSLVGEAENQ 181

Query: 615  GATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPS 436
            G TF+YN+TV+GGHLEG  I +H++++  + +  G    QPEL+L+P LVVNS GLSSP 
Sbjct: 182  GTTFTYNSTVIGGHLEGSEICLHVTETDRLTEWKGTSILQPELLLIPKLVVNSTGLSSPV 241

Query: 435  LAKSFKGLDSRVIPPSYYARGCYFTLSNTKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVK 256
            LAK F G  S V+PP+YYARGCYFTLSNTK  PF  LIYPIPEDGG+GVHVT+DL+G VK
Sbjct: 242  LAKRFNGPKSGVVPPAYYARGCYFTLSNTKNSPFRRLIYPIPEDGGIGVHVTIDLNGQVK 301

Query: 255  FGPDVEWIGELDCISSFLNKFDYSVSSDHVQKFYPDIRRYYPNLKEGSLEPGYAGIRPKL 76
            FGP+VEWI  +D ISSF NK+DYSV+++  ++FYP+IR+YYPNLK+GSLEPGY+GIRPKL
Sbjct: 302  FGPNVEWIDSVDDISSFQNKYDYSVNANRAERFYPEIRKYYPNLKDGSLEPGYSGIRPKL 361

Query: 75   SGPGQPPVDFVIQGEDIHGVRGLIN 1
            SGP QPP DFVIQGEDIHGV GLIN
Sbjct: 362  SGPLQPPSDFVIQGEDIHGVPGLIN 386


>gb|AFK34722.1| unknown [Lotus japonicus]
          Length = 418

 Score =  521 bits (1341), Expect = e-145
 Identities = 253/373 (67%), Positives = 304/373 (81%)
 Frame = -2

Query: 1119 LLETIPKENVDCXXXXXXXXXXXXARDLALKGRDVLVIESAPTFGSGTSSRNSEVIHAGI 940
            +++++P+E VDC            AR LALKGR+VLVIES  TFG+GTSSRNSEVIHAGI
Sbjct: 21   IMDSVPREKVDCVVIGAGVVGIAVARALALKGREVLVIESGSTFGTGTSSRNSEVIHAGI 80

Query: 939  YYPPNSLKAIFCSRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKLIVLLSRGRENGV 760
            YYP +SLKAIFC RGR++LY+YC+ H +PHKQ+GKLIVAT SSEI KL  +L+ G +NGV
Sbjct: 81   YYPRDSLKAIFCVRGREMLYEYCSMHDIPHKQVGKLIVATRSSEIPKLNDILNHGIQNGV 140

Query: 759  EGLRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLVGEAENHGATFSYNTTVVG 580
            + L M+ G +AM+MEPEL+C+KA+LSP SGIVD+H+LMLSLVGEAENH  TF+YN+TV+G
Sbjct: 141  DSLEMINGAKAMKMEPELQCVKAILSPLSGIVDSHSLMLSLVGEAENHRTTFTYNSTVIG 200

Query: 579  GHLEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRV 400
            GHLEG  I +HI ++K +++  G     PELILVP L+VNS GLS+P+LAK F  L S V
Sbjct: 201  GHLEGKEICLHILETKCLKEWDGTSILHPELILVPKLLVNSTGLSAPALAKRFTSLQSEV 260

Query: 399  IPPSYYARGCYFTLSNTKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELD 220
            IPPSYYARGCYFTLS+TK  PF HLIYPIPEDGGLGVHVTLDL+G +KFGPDVEWI  +D
Sbjct: 261  IPPSYYARGCYFTLSDTKASPFRHLIYPIPEDGGLGVHVTLDLNGQIKFGPDVEWIDGVD 320

Query: 219  CISSFLNKFDYSVSSDHVQKFYPDIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFVI 40
             ISSF NKFDYSV ++  ++FYP IR YYPNLK+ +LEPGYAGIRPKLS PGQ PVDFVI
Sbjct: 321  DISSFQNKFDYSVHANRAEQFYPAIRSYYPNLKDRALEPGYAGIRPKLSRPGQSPVDFVI 380

Query: 39   QGEDIHGVRGLIN 1
            QGEDIHG+ GL+N
Sbjct: 381  QGEDIHGIPGLVN 393